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PubMed | a Laboratoire des Plantes Extremophiles Biotechnologic Center Borj Cedria Technopark
Type: Journal Article | Journal: Natural product research | Year: 2014

The chemical composition of eight Tunisian Rosmarinus officinalis L. populations (A-H) from different bioclimatic areas has been examined by gas chromatography (GC) and GC-mass spectrometry. The essential oils are characterised by high amounts of oxygenated monoterpenes (58.2-71.7%) followed by monoterpene hydrocabons (15.1-26.7%). 1,8-Cineole, camphor, -pinene and borneol are the main representative components. The antioxidant activity was investigated by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing ability power assay and -carotene bleaching test. Samples showed antiradical activity by inhibiting DPPH radical with IC50 values ranging from 375.3 to 592.8gmL(-1) for samples F and A, respectively. Sample A also showed the most promising activity in -carotene bleaching test (IC50 of 31.9gmL(-1)). The essential oils were also screened for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity. Sample G showed the highest activity against AChE (IC50 of 64.7gmL(-1)) while sample D (IC50 of 29.5 gmL(-1)) exhibited the most potent activity against BChE.


PubMed | a Laboratoire des Plantes Extremophiles Biotechnologic Center Borj Cedria Technopark
Type: Journal Article | Journal: Natural product research | Year: 2013

This study analyses the chemical composition and in vitro antioxidant activity of both the essential oil and the 80% aqueous acetone extract of Tetraclinis articulata leaves. The GC-MS analysis of the essential oil identified 66 components that comprise 93.5% of the oil. The major constituents of the oil are: bornyl acetate (31.4%), -pinne (24.5%) and camphor (20.3%). Antioxidant activities of the samples were determined using four different test systems, namely DPPH, -carotene/linoleic acid, reducing power and metal chelating activity assay. Test results from the DPPH system showed the strongest radical scavenging activity was exhibited by the 80% aqueous acetone extract (IC=5.5gmL), which was two times higher than the positive control (BHT). The amount of the total phenolics, flavonoids and condensed tannins was very high in the 80% aqueous acetone extracts. The correlation between the antioxidant activity potential and total phenolic level of the extract was noted.

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