De Rosa V.,Laboratoire des L e Sions des Acides Nuclear e Iques |
Erkekoglu P.,Laboratoire des L e Sions des Acides Nuclear e Iques |
Erkekoglu P.,Hacettepe University |
Forestier A.,Laboratoire des L e Sions des Acides Nuclear e Iques |
And 5 more authors.
Free Radical Research | Year: 2012
Epidemiological studies have demonstrated an inverse relationship between selenium (Se) intake and cancer incidence and/or mortality. However, the molecular mechanisms underlying the cancer chemopreventive activity of Se compounds remain largely unknown. The objective of this study was to investigate the effect of low doses of Se on the stimulation of DNA repair systems in response to four different qualities of DNA damage. P53-proficient LNCaP human prostate adenocarcinoma cells were grown either untreated or in the presence of low concentrations of two Se compounds (30° nM sodium selenite, or 10μM selenomethionine) and exposed to UVA, H 2O 2, methylmethane sulfonate (MMS) or UVC. Cell viability as well as DNA damage induction and repair were evaluated by the alkaline Comet assay. Overall, Se was shown to be a very potent protector against cell toxicity and genotoxicity induced by oxidative stress (UVA or H 2O 2) but not from the agents that induce other types of deleterious lesions (MMS or UVC). Furthermore, Se-treated cells exhibited increased oxidative DNA repair activity, indicating a novel mechanism of Se action. Therefore, the benefits of Se could be explained by a combination of antioxidant activity, the reduction in DNA damage and the enhancement of oxidative DNA repair capacity. © 2012 Informa UK, Ltd. Source