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Nasri N.,Tunis el Manar University | Tlili N.,Tunis el Manar University | Triki S.,Tunis el Manar University | Elfalleh W.,Laboratoire dAridoculture et cultures oasiennes | And 2 more authors.
Journal of Essential Oil Research

The composition of the essential oil hydro-distilled from needles of Pinus pinea L. trees, collected from six populations coming from Northern Tunisia was determined by GC and GC/MS analysis. Fifty-seven components, accounting for 94.4-99.9% of the oil were identified. As expected, the majority of the identified metabolites were found to be monoterpenes and sesquiterpenes. Monoterpenes account about 76% with 34 of 57 compounds identified. The principal component was limonene (35.9%) followed by α-pinene (6.4%). Sesquiterpenes (ca.19%) were mainly β-cubebene (1.8%), β-caryophyllene (1.7%), α humulene (1.7%) and β-farnesene (1.7%). Diterpenes (1.4%) were present in few concentrations mainly at one population. Data projection on axes of principal component analysis from P. pinea samples showed significant geographic polymorphism between Tunisian populations. This suggests that, using an appropriate wide-range sample of P. pinea populations, terpenes could be used to study the genetic diversity of P. pinea. This species has never shown a noticeable genetic variability using microsatellites, fatty acids or allozymes. © 2011 Allured Business Media. Source

Hannachi H.,Laboratoire dAridoculture et cultures oasiennes | Elfalleh W.,Laboratoire dAridoculture et cultures oasiennes | Ennajeh I.,Laboratoire dAridoculture et cultures oasiennes | Laajel M.,Laboratoire dAridoculture et cultures oasiennes | And 4 more authors.
Journal of Medicinal Plant Research

This study reported investigations on phytochemical screening and antioxidants activities of seeds from seven Acacia species. Storage proteins and mineral contents were determined. The seed extracts of Acacia species were evaluated for their total phenols, flavonoids, carotenoids contents and total antioxidant capacity assessed by 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2- diphenyl-1- picrylhydrazyl (DPPH) assays. Total protein content ranged from 99.49 (Acacia cyclops) to 142.77 (Acacia cyanaphylla) mg/g dry weight basis (DW) according to the Acacia species, having an average of 120.41 mg/g DW. The albumin constitutes the major fraction presenting 31.11%, followed by globulin (27.36%), prolamin (22.27%) and glutelin (19.27%). The Acacia seeds were rich in minerals. The total phenolic compounds (TPC) determinate ranged from 154.47 (A. cyclops) to 1217.99 (Acacia horrida) with an average of 632.40 mg gallic acid equivalents/100 g DW. The total flavonoid contents have an average of 11.21 mg rutin equivalents/100 g DW, and the carotenoids mean is 4.23 mg/kg. The Acacia seed extracts have an antioxidant activity reflected by the ABTS and DPPH tests. The Duncan's test showed significant difference of proteinic, phenolics, flavonoid and carotenoid contents according to the Acacia species. Therefore, natural components from Acacia seeds could be incorporated as food ingredient, without causing detrimental effects to the food's palatability and the functional ingredient's efficacy. © 2011 Academic Journals. Source

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