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La Tour-du-Pin, France

Bouali M.,French Atomic Energy Commission | Bouali M.,Laboratoire dAnalyses | Pelletier E.,French Atomic Energy Commission | Chaussonnerie S.,French Atomic Energy Commission | And 4 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Performance of biological wastewater treatment systems may be related to the composition and activity of microbial populations they contain. However, little information is known regarding microbial community inhabiting these ecosystems. The purpose of this study was to investigate archaeal and bacterial diversity, using cultivation-independent molecular techniques, in a constructed wetland receiving domestic wastewater. Two 16S rRNA gene libraries were constructed using total genomic DNA and amplified by PCR using primers specific for archaeal and bacterial domains. A high microbial diversity was detected. The Proteobacteria phylum is the most abundant and diversified phylogenetic group representing 31.3 % of the OTUs, followed by the Bacteroidetes (14.8 %), Planctomycetales (13.8 %), Actinobacteria(12 %), and Chloroflexi (8.2 %). Sequences affiliated with minor phylogenetic divisions such as the TM7, Nitrospira, OP10, and BRC1 are represented by <6 % of total OTUs. The Archaea domain was represented by the Thaumarchaeota phylum dominated by the Candidatus Nitrososphaera genus. © 2012 Springer-Verlag.

Merrien A.,Laboratoire dAnalyses | Krouti M.,Laboratoire dAnalyses | Diaz J.,Laboratoire dAnalyses
OCL - Oleagineux Corps Gras Lipides | Year: 2010

The traceability requirements and allotment bring collectors or oilseed industry to seek for rapid, easy to implement, methods to determine the quality of productions. From this point of view, infrared methods offer possibilities. It's necessary however to be aware of both its advantages (fast, simple, multi-criteria non-destructive), and also limits: this indirect method requires constant validation by reference methods, and wide ranges ofcontinuous and referenced values according to standards, effects "matrix" (eg Sunflower). Beyond the apprehension of some criteria on delivery (oil content), these methods are useful to breeders because they allow them a rapid screening of their equipment.

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