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Raache R.,University of Science and Technology Houari Boumediene | Raache R.,Service Route | Hennachi R.,Mustapha University Hospital Center | Amroune H.,Service Route | And 12 more authors.
Journal Francais d'Ophtalmologie | Year: 2013

Background: Diabetic retinopathy (DR) is the most frequent microvascular complication of type I diabetes (T1D). Some well-controlled type I diabetics may develop DR, while other poorly-controlled diabetics do not develop DR. This might be explained by certain susceptibility genes or protective genes. The purpose of our study is to search for any association between the HLA class I and II markers and DR in the Algerian population. Patients and methods: This study was carried out in 52 T1D subjects with and without DR compared to 140 healthy controls. HLA typing was performed using the " microlymphocytotoxicity" technique. Results: The frequency of HLA-A29 and HLA-DR9 antigens is higher in T1D with DR compared to T1D without DR and to controls with frequencies of HLA-A29 (59.26% vs. 0%, OR = ∞, pc = 4.6 × 10-7), (59.26% vs. 5.66%, OR = 24.24, pc = 7.6 × 10-10) and HLA-DR9 (29.63% vs. 0%, OR = ∞, pc = 1.310-3), (29.63% vs. 4.29%, OR = 9.40, pc = 7.010-5) respectively. However, the frequency of HLA-B49 antigen is significantly lower in T1D with DR than in T1D without DR (3.7% vs. 28%, OR = 0.10, pc = 8.8 × 10-3) and compared to controls (3.7% vs. 22.64%, OR = 0.13, pc = 0.011). Conclusion: HLA-A29 and HLA-DR9 antigens are probably markers of susceptibility for DR while HLA-B49 antigen is probably associated with a protective effect in the Algerian population. © 2012 Elsevier Masson SAS. Source


Raache R.,University of Science and Technology Houari Boumediene | Raache R.,Institute Pasteur dAlgerie | Belanteur K.,Institute Pasteur dAlgerie | Amroun H.,Institute Pasteur dAlgerie | And 9 more authors.
Clinical and Vaccine Immunology | Year: 2012

Major histocompatibility complex class I chain-related gene A (MICA-129) dimorphism was investigated in 73 autoimmune diabetes patients (type 1 diabetes and latent autoimmune diabetes in adults) and 75 controls from Algeria. Only MICA-129 Val allele and MICA-129 Val/Val genotype frequencies were higher among patients than in the control group. Statistical analysis of the estimated extended HLA-DR-DQ-MICA haplotypes shown that individual effects of MICA alleles on HLA-DQ2-DR3-MICA-129 Val/Val and HLA-DQ8-DR4-MICA-129 Val/Val haplotypes were significantly higher in patients than in the control groups. These preliminary data might suggest a relevant role of MICA-129 Val/Val single nucleotide polymorphism (weak/weak binders of NKG2D receptor) in the pathogenesis of T1D and LADA. Copyright © 2012, American Society for Microbiology. All Rights Reserved. Source


Gouaref I.,University of Science and Technology Houari Boumediene | Bellahsene Z.,Laboratoire Central Of Biologie | Zekri S.,Service de Medecine Interne | Alamir B.,Center National Of Toxicologie | Koceir E.-A.,University of Science and Technology Houari Boumediene
Annales de Biologie Clinique | Year: 2016

The relationship between trace elements (TE) and essential hypertension (EH) is subtle and complex. This relationship is mediated by endothelial dysfunction, insulin resistance, oxidative stress (OS) and athero-inflammatory state. The aim of this study was to examine the TE impact; particularly selenium (Se), zinc (Zn), copper (Cu) and manganese (Mn) as predictive type 2 diabetes biomarkers in a hypertensive subject. The study was undertaken on 400 adult patients (40-60 years), who were divided in 4 groups: hypertensive (H), type 2 diabetes (T2D), hypertensive-diabetic (HD) and healthy group. Patients were phenotyped regarding their metabolic syndrome profile using the NCEP/ATPIII criteria. Hypertension was defined as systolic (SBP) and diastolic (DBP) blood pressure ≥140/90 mmHg, respectively. The SBP and DBP measurements by electronic blood pressure using Omron 705 CP® type. Insulin resistance was assessed by Homa-IR model. Metabolic and inflammatory parameters were determined by Cobas Integra®; the TE investigated by mass spectrometric atomic absorption; the OS markers evaluated by Randox kits. Serum Se concentrations are reduced in all groups, concomitantly with a marked depletion GPx activity in the HD group. However, Zn levels were decreased than in H and HD groups, but unchanged in T2D group. In contrast, Mn levels are increased in all groups; whereas the Cu levels increased only in H and HD groups, concomitantly with cytosolic SOD-Cu/Zn and mitochondrial SOD-Mn depletion. The Zn/Cu ratio decreases significantly in hypertensive group but not in diabetics groups. It appears that Zn/Cu ratio reflects the transition from hypertension phase to hypertension associated with T2D. Ultimately, TE plays an important role in the hypertension pathophysiology and can be considered as predictive T2D biomarkers in hypertensive patients. Copyright © 2016 JLE. Source


Tahiat A.,Laboratoire Central Of Biologie | Djidjik R.,Laboratoire Central Of Biologie | Djidjik R.,Laboratoire Of Recherche En Immunogenetique Et Immunopathologie Ligip | Boushaki S.,Laboratoire Central Of Biologie | And 9 more authors.
Pathologie Biologie | Year: 2014

Purpose: Common variable immunodeficiency (CVID) is the commonest symptomatic primary immunodeficiency. It is characterized by a defect of antibody production, recurrent respiratory tract infections and increased occurrence of auto-immune discords and lymphoproliferative disease. Methods: This retrospective study was conducted on 29patients fulfilling the classical CVID definition. Blood tests included immunoglobulin measurement and lymphocyte subpopulations phenotyping. Results: This study includes 29patients. The mean age at diagnosis was 23. years. Recurrent upper and lower bacterial respiratory tract infections were common in almost all patients. Five patients developed auto-immune conditions and six had lymphoproliferative disease. Decreased IgG was found in almost all patients. Low IgA and IgM levels were found in 89.6% and 65.5% of cases respectively. Abnormal T and/or B phenotype was found in 75% of cases; the most common abnormalities were decreased circulating B (54.2%) and T CD4+ (41.7%) cells and inversion of the CD4/CD8 ratio (70.8%). Patients with decreased circulating B and T CD4+ cells were significantly more likely to have auto-immune cytopenias and lymphoproliferative disease. Conclusions: Our study confirms the heterogeneity of CVID. A patient's classification is necessary to define homogeneous groups of patients and to characterize specific molecular abnormalities in each group. © 2014 Elsevier Masson SAS. Source


Tahiat A.,Laboratoire Central Of Biologie | Djidjik R.,Laboratoire Central Of Biologie | Boushaki S.,Laboratoire Central Of Biologie | Cherguelaine K.,Laboratoire Central Of Biologie | And 8 more authors.
Pathologie Biologie | Year: 2014

Purpose: Common variable immunodeficiency (CVID) is the commonest symptomatic primary immunodeficiency. It is characterized by a defect of antibody production, recurrent respiratory tract infections and increased occurrence of auto-immune discords and lymphoproliferative disease. Methods: This retrospective study was conducted on 29 patients fulfilling the classical CVID definition. Blood tests included immunoglobulin measurement and lymphocyte subpopulations phenotyping. Results: This study includes 29 patients. The mean age at diagnosis was 23 years. Recurrent upper and lower bacterial respiratory tract infections were common in almost all patients. Five patients developed auto-immune conditions and six had lymphoproliferative disease. Decreased IgG was found in almost all patients. Low IgA and IgM levels were found in 89.6 % and 65.5 % of cases respectively. Abnormal T and/or B phenotype was found in 75 % of cases; the most common abnormalities were decreased circulating B (54.2 %) and T CD4+ (41.7 %) cells and inversion of the CD4/CD8 ratio (70.8 %). Patients with decreased circulating B and T CD4+ cells were significantly more likely to have auto-immune cytopenias and lymphoproliferative disease. Conclusions: Our study confirms the heterogeneity of CVID. A patient's classification is necessary to define homogeneous groups of patients and to characterize specific molecular abnormalities in each group. © 2014 Elsevier Masson SAS. All rights reserved. Source

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