Laboratoire central

Khroub, Algeria

Laboratoire central

Khroub, Algeria

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Issolah R.,INRAA | Tahar A.,Annaba University | Alane F.,British Petroleum | Sadi S.,Laboratoire Central | And 4 more authors.
Journal of Biological Sciences | Year: 2014

As part of the evaluation and the development of plant genetic resources of fodder and pastoral interest in Algeria, ten populations of sulla (Sulla coronaria (L.) Medik. Syn. Hedysarum coronarium L.) were subject to an assessment. The growth and the chemical composition of the populations were analyzed in this framework. The field trial is a randomized complete block design with four replications. Several parameters (seeds thousand weight, emergence of seedlings, number of plants per square meter, maximum height, maximum width, bloom, cutting date, green matter, dry matter, mineral matter, organic matter, crude fiber, fat, phosphorus, calcium, total nitrogen, Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF), lignin, hemicellulose) have been determined. Two ecological factors (altitude, rainfall) were also taken into account. The variance analysis indicated a significant variation between the populations regarding eight variables linked to the growth and the chemical composition of the plant. Many significant relationships were also highlighted. The results of the correlation matrix indicated also that the total nitrogen content is negatively correlated with the number of plants per square meter and the final height. Overall, the results of principal component analysis showed a good distribution of variables, particularly, the final width, the hemicellulose and the NDF. The descriptive analysis signalized also the part of the altitude and the rainfall characterizing the natural habitats of the populations. This study permitted a better understanding of the nature of variation established within this species. Sulla can play a significant role in the socio-economic and environmental development of rural communities. © 2014 Asian Network for Scientific Information.


Issolah R.,INRAA | Tahar A.,Annaba University | Sadi S.,Laboratoire Central | Adjabi M.,Laboratoire Central | And 3 more authors.
Journal of Biological Sciences | Year: 2016

Background and Objective: As part of the valorization of plant genetic resources of fodder and pastoral interest in Algeria, ten populations of subterraneun clover (Trifolium subterraneum L.) were subject to an evaluation. The objective of this study is the characterization of the Algerian populations of subterraneun clover on quantitative and qualitative level. This would permit to increase the number of fodder species used in the different areas of the country for developing the livestock. Materials and Methods: The field trial was a randomized complete block design with four replications. Then, the chemical composition of the populations was analysed at the laboratory. In this framework, several parameters were determined (Thousand seeds weight, seedlings, height, width, flowering, cutting date, green matter, mineral matter, organic matter, crude fiber, fat, phosphorus, calcium, total nitrogen, acid detergent fiber, neutral detergent fiber, lignin and hemicellulose). Two ecological factors (Altitude and rainfall) characterizing the natural habitat of the populations were considered. The data obtained have undergone statistical treatments (ANOVA/Tukey method). Then, the correlations and the principal component analysis were used for a better interpretation of the relations established between the different populations and the variables. Results: The variance analysis indicated that there is some variation in three variables linked to the cutting date, the thousand seed weight and the yield of green matter. It seems that there is no link between the variables and the ecological factors. The principal component analysis indicated that the calcium and the vegetative development in width are the variables, the best represented. Conclusion: Overall, the variables related to the chemical composition contribute more than those related to the vegetative development (number of variables), but the probabilities corresponding to the vegetative development are the most significant. This study would contribute to select the best populations of subterraneun clover in order to introduce them in the different agro-edapho-climatic areas, specially in the Mountains of the North East of Algeria, which is the most watered zone in the country. © 2016 R. Issolah et al.


Adonis-Koffy L.,Service de pediatrie medicale | Diarrassouba G.,Service de pediatrie medicale | Mouba J.,Service de pediatrie medicale | Ake-Assi M.-H.,Service de pediatrie medicale | And 3 more authors.
Archives de Pediatrie | Year: 2010

Cryptococcal meningitidis is rare, occurring particularly in adults infected with the HIV virus. The authors report the cases of 2 immunocompetent girls who were 3 and 13. years old. The authors describe the risk factors, the clinical symptoms, and the biological characteristics of the cerebrospinal fluid. © 2010.


Bouzenoune F.,Laboratoire central | Kellab Debbih K.,Service depidemiologie et de medecine preventive | Boudersa F.,Service de medecine interne | Kouhil S.,Laboratoire central | Nezzar N.,Laboratoire central
Medecine et Maladies Infectieuses | Year: 2011

Introduction: Typhoid fever is a food- and water-borne disease, caused by Salmonella enterica serovar Typhi, responsible for high rates of morbidity and mortality in developing countries. Typhoid is also a public health problem in Algeria. Antimicrobial susceptibility surveillance must be applied to prevent the emergence of multidrug resistant strains. Patients and methods: We studied the incidence of S. enterica serovar Typhi isolated from blood cultures in the Ain M'lila public hospital (Algeria), between 2005 and 2008. Blood cultures were performed in the febrile stage of infection and positive samples were identified by biochemical and antigenic tests. Susceptibility to ampicillin, cotrimoxazole, chloramphenicol and nalidixic acid was tested by antibiogram. Results: One hundred and seventy-eight strains were isolated from blood cultures between 2005 and 2008. They were all susceptible to the antibiotics tested. Discussion: Typhoid fever incidence has decreased in Algeria. In our region, it comes by outbreaks during the summer season, with no sporadic cases between the peaks. In our study, S. enterica serovar Typhi was still susceptible to antimicrobials despite the worldwide emergence of multidrug resistant strains. Conclusion: A regular surveillance of Salmonella typhi antibiotic susceptibility is mandatory. © 2010.


Choukri M.,Laboratoire Central | Taheri H.,Service de Gynecologie obstetrique | Seddik R.,Laboratoire Central | Benkirane S.,Sina | And 4 more authors.
Annales de Biologie Clinique | Year: 2013

Recent classifications of non-Hodgkin's lymphomas based on combination of morphologic, immunophenotypic, and cytogenetic criteria have individualized mantle cell lymphoma (MCL). This clinico-biological entity which accounts for 3 to 10% of all non-Hodgkin's lymphomas, now appears to be a biological and therapeutic model for the understanding and treatment of hematologic malignancies. The present study consisting of two cases of MCL collated at laboratory of hematology of Rabat Ibn Sina hospital. The morphological appearance of MCL is characterized by diffuse or nodular lymph infiltration in the mantle zone, the osteo-medullary biopsy shows an interstitial infringement characterized by the presence of lymphocytes resembling centrocytes with cleaved and angular nuclei, dispersed chromatin, inconspicuous nucleoli and scanty cytoplasm. The flow cytometry showed immunophenotype positive for surface Ig, CD19, CD20, CD22, CD79b, CD5 and cyclin D1, and negative for CD10, CD23 and CD25. In conclusion, the methods of diagnosis and prognosis evaluation of mantle cell lymphoma are based on the nodular, medullary and blood morphology, the immunophenotypic, cytogenetic and molecular study of neoplastic cells.


Tali-Maamar H.,Institute Pasteur dAlgerie | Laliam R.,Institute Pasteur dAlgerie | Bentchouala C.,Service de microbiologie | Touati D.,Laboratoire Mere Enfant | And 8 more authors.
Vaccine | Year: 2012

Introduction: Pneumococcal infections are a major public health problem because of the virulence of this bacterium and its ability to develop resistance. Material and method: Two hundred and ninety-four strains of Streptococcus pneumoniae were isolated from sterile (56.8%) and non-sterile samples (43.2%), from January 2001 to July 2010. Results: The interpretation of antibiotic susceptibility testing, according to CLSI criteria (M100-S21 2011), yielded a 25.2% overall resistance to penicillin, with 23.5% of strains isolated from CSF (meningitis), and only 1.7% in other samples. Resistance to cefotaxime was 8.1% (including 4.4% at a high level). The most common serotypes were: 14 (19.5%), 23F (9.7%), 6B (9.3%), 19F (5.4%), and serotype 1 (5%). The percentage of these serotypes isolated from normally sterile sites in children under 5 years of age was 31.25% for 14, 10.4% for 23F, 8.3% for 19F, 6.25% for 6B, and 4.2% for serotype 1. The theoretical vaccinal coverage against invasive infections in children under 2 years of age was 61.5%, 69.2%, and 76.9% for the 7-valent, 10-valent, and 13-valent conjugate vaccines, respectively. Penicillin non-susceptible Streptococcus pneumoniae (PNSP) strains accounted for 67.1, 68.6, and 72.8% for each of these three vaccines. Conclusion: There was a variation of serotype rates compared to previous studies. The increase in pneumococcal antibiotic resistance is concerning, particularly for the treatment of pneumococcal infections in children and infants. Pneumococcal vaccination is not compulsory yet in Algeria. © 2011 Elsevier Ltd.


Diallo A.-M.,British Petroleum | Diallo M.-M.,British Petroleum | Balde N.-M.,British Petroleum | Diakite M.,Laboratoire Central | And 6 more authors.
Medecine des Maladies Metaboliques | Year: 2012

Changes in traditional habits and way of life and increasing urbanization in developing countries expose populations to an increased risk of cardiovascular diseases which has results in the metabolic syndrome concept. Within metabolic syndrome, type 2 diabetes appears as "the tip of the iceberg". In this first work carried out in Guinea in a cohort of 272 type 2 diabetic patients, metabolic syndrome prevalence was of 56%, obesity (abdominal obesity and/or body mass index [BMI] ≥30 kg/m2) and hypertension were the most frequent criteria among those of the metabolic syndrome definition (International Diabetes Federation, IDF) associated with type 2 diabetes.Thus, optimal type 2 diabetic patients' management in sub-Saharan Africa should be based on a more global approach. © 2012 - Elsevier Masson SAS - Tous droits réservés.


Tali-Maamar H.,Institute Pasteur dAlgerie | Laliam R.,Institute Pasteur dAlgerie | Bentchouala C.,Service de microbiologie | Touati D.,Laboratoire Mere Enfant | And 8 more authors.
Medecine et Maladies Infectieuses | Year: 2012

Introduction: Pneumococcal infections are a major public health problem because of the virulence of this bacterium and its ability to develop resistance. Material and method: Two hundred and ninety-four strains of Streptococcuspneumoniae were isolated from sterile (56.8%) and non-sterile samples (43.2%), from January 2001 to July 2010. Results: The interpretation of antibiotic susceptibility testing, according to CLSI criteria (M100-S21 2011), yielded a 25.2% overall resistance to penicillin, with 23.5% of strains isolated from CSF (meningitis), and only 1.7% in other samples. Resistance to cefotaxime was 8.1% (including 4.4% at a high level). The most common serotypes were: 14 (19.5%), 23F (9.7%), 6B (9.3%), 19F (5.4%), and serotype 1 (5%). The percentage of these serotypes isolated from normally sterile sites in children under 5 years of age was 31.25% for 14, 10.4% for 23F, 8.3% for 19F, 6.25% for 6B, and 4.2% for serotype 1. The theoretical vaccinal coverage against invasive infections in children under 2 years of age was 61.5%, 69.2%, and 76.9% for the 7-valent, 10-valent, and 13-valent conjugate vaccines, respectively. Penicillin non-susceptible. Streptococcuspneumoniae (PNSP) strains accounted for 67.1, 68.6, and 72.8% for each of these three vaccines. Conclusion: There was a variation of serotype rates compared to previous studies. The increase in pneumococcal antibiotic resistance is concerning, particularly for the treatment of pneumococcal infections in children and infants. Pneumococcal vaccination is not compulsory yet in Algeria. © 2011 Elsevier Masson SAS.


PubMed | Laboratoire central
Type: Journal Article | Journal: Medecine et maladies infectieuses | Year: 2011

Typhoid fever is a food- and water-borne disease, caused by Salmonella enterica serovar Typhi, responsible for high rates of morbidity and mortality in developing countries. Typhoid is also a public health problem in Algeria. Antimicrobial susceptibility surveillance must be applied to prevent the emergence of multidrug resistant strains.We studied the incidence of S.enterica serovar Typhi isolated from blood cultures in the Ain Mlila public hospital (Algeria), between 2005 and 2008. Blood cultures were performed in the febrile stage of infection and positive samples were identified by biochemical and antigenic tests. Susceptibility to ampicillin, cotrimoxazole, chloramphenicol and nalidixic acid was tested by antibiogram.One hundred and seventy-eight strains were isolated from blood cultures between 2005 and 2008. They were all susceptible to the antibiotics tested.Typhoid fever incidence has decreased in Algeria. In our region, it comes by outbreaks during the summer season, with no sporadic cases between the peaks. In our study, S.enterica serovar Typhi was still susceptible to antimicrobials despite the worldwide emergence of multidrug resistant strains.A regular surveillance of Salmonella typhi antibiotic susceptibility is mandatory.

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