Laboratoire Biosante et Nanoscience EA 4203

Montpellier, France

Laboratoire Biosante et Nanoscience EA 4203

Montpellier, France
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Collart Dutilleul P.-Y.,Laboratoire Biosante et Nanoscience EA 4203 | Fonseca C.G.,Autonomous University of San Luis Potosi | Zimanyi L.,Laboratoire Biosante et Nanoscience EA 4203 | Zimanyi L.,Hungarian Academy of Sciences | And 6 more authors.
Journal of Biomedical Materials Research - Part B Applied Biomaterials | Year: 2013

A new strategy to improve silicon-based endodontic treatment tightness by dentine hydrophobization is presented in this work: root dentine was silanized to obtain a hydrophobic dentine-sealer interface that limits fluid penetration. This strategy was based on the grafting of aliphatic carbon chains on the dentine through a silanization with the silane end groups [octadecyltrichlorosilane (OTS) and octadecyltriethoxysilane]. Dentine surface was previously pretreated, applying ethylenediaminetetraacetic acid and sodium hypochlorite, to expose hydroxyl groups of collagen for the silane grafting. Collagen fibers exposure after pretreatment was visible with scanning electron microscopy, and Fourier transform infrared (FTIR) spectroscopy showed their correct exposition for the silanization (amide I and II, with 1630, 1580, and 1538 cm-1 peaks corresponding to the vibration of C=O and C-N bonds). The grafting of aliphatic carbon chains was confirmed by FTIR (peaks at 2952 and 2923 cm-1 corresponding to the stretching of C-H bonds) and by the increasing of the water contact angle. The most efficient hydrophobization was obtained with OTS in ethyl acetate, with a water contact angle turning from 51° to 109°. Gas and liquid permeability tests showed an increased seal tightness after silanization: the mean gas and water flows dropped from 2.02 × 10-8 to 1.62 × 10-8 mol s-1 and from 10.8 × 10-3 to 5.4 × 10-3 μL min -1, respectively. These results show clear evidences to turn hydrophilic dentine surface into a hydrophobic surface that may improve endodontic sealing. © 2013 Wiley Periodicals, Inc.


Collart Dutilleul P.-Y.,Laboratoire BioSante et Nanoscience EA 4203 | Thonat C.,Laboratoire BioSante et Nanoscience EA 4203 | Jacquemart P.,Laboratoire BioSante et Nanoscience EA 4203 | Cuisinier F.,Laboratoire BioSante et Nanoscience EA 4203 | And 2 more authors.
L' Orthodontie française | Year: 2012

Stem cells discovery and their potential have led to the emergence of new forms of therapy with the development of bio-engineering cell and tissue methods underlying future medicine. The availability of stem cells and their preservation thus become an issue for everyone's health. Among the different sources of stem cells, those in the dental pulp have the advantage of being pluripotent, they can be cryopreserved and stored for long periods without losing their multiplication and differentiation capacities and finally they are easily accessible. The wisdom or natal teeth extracted for medical reasons are an opportunity for everyone to preserve stem cells for an autologous use. Biobanks authorized and specialized in the preparation and storage of pulp stem cells provide access to autologous regenerative medicine of tomorrow. © EDP Sciences, SFODF, 2012.


PubMed | Laboratoire Biosante et Nanoscience EA 4203
Type: Journal Article | Journal: Journal of biomedical materials research. Part B, Applied biomaterials | Year: 2013

A new strategy to improve silicon-based endodontic treatment tightness by dentine hydrophobization is presented in this work: root dentine was silanized to obtain a hydrophobic dentine-sealer interface that limits fluid penetration. This strategy was based on the grafting of aliphatic carbon chains on the dentine through a silanization with the silane end groups [octadecyltrichlorosilane (OTS) and octadecyltriethoxysilane]. Dentine surface was previously pretreated, applying ethylenediaminetetraacetic acid and sodium hypochlorite, to expose hydroxyl groups of collagen for the silane grafting. Collagen fibers exposure after pretreatment was visible with scanning electron microscopy, and Fourier transform infrared (FTIR) spectroscopy showed their correct exposition for the silanization (amide I and II, with 1630, 1580, and 1538 cm peaks corresponding to the vibration of C=O and C--N bonds). The grafting of aliphatic carbon chains was confirmed by FTIR (peaks at 2952 and 2923 cm corresponding to the stretching of C--H bonds) and by the increasing of the water contact angle. The most efficient hydrophobization was obtained with OTS in ethyl acetate, with a water contact angle turning from 51 to 109. Gas and liquid permeability tests showed an increased seal tightness after silanization: the mean gas and water flows dropped from 2.02 10 to 1.62 10 mol s and from 10.8 10 to 5.4 10 L min, respectively. These results show clear evidences to turn hydrophilic dentine surface into a hydrophobic surface that may improve endodontic sealing.


PubMed | Laboratoire BioSante et Nanoscience EA 4203
Type: Journal Article | Journal: L' Orthodontie francaise | Year: 2012

Stem cells discovery and their potential have led to the emergence of new forms of therapy with the development of bio-engineering cell and tissue methods underlying future medicine. The availability of stem cells and their preservation thus become an issue for everyones health. Among the different sources of stem cells, those in the dental pulp have the advantage of being pluripotent, they can be cryopreserved and stored for long periods without losing their multiplication and differentiation capacities and finally they are easily accessible. The wisdom or natal teeth extracted for medical reasons are an opportunity for everyone to preserve stem cells for an autologous use. Biobanks authorized and specialized in the preparation and storage of pulp stem cells provide access to autologous regenerative medicine of tomorrow.

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