Hssaine A.,Laboratoire Aliments |
Gharbi J.,University of Monastir |
Harrath R.,University of Monastir |
Harrak R.,Laboratoire Regional Du Diagnostic Epidemiologique Et Of Lhygiene Du Milieu |
And 3 more authors.
African Journal of Microbiology Research | Year: 2011
Enteroviruses are among the most common viruses infecting the human intestine; they are very widespread in nature and resistant to external agents. They are eliminated in the faeces and contaminate water and food. These viruses cause various clinical syndromes and constitute a big public health problem. The aim of our study was to search for enteroviruses in the water samples. For this, a total of 225 L of tap water coming from the national network office for drinking water (ONEP) and 18 samples of waste water originating from Marrakech city were studied. These samples were concentrated, treated with polyethylene glycol 6000 and then analyzed by RT-PCR. Only two samples were found to be positive for enteroviruses by RT-PCR among the 18 waste water samples analyzed, which gave a rate of 11.11%. On the other hand, no positive samples were found in the tap water. This study made it possible on the one hand to apply for the first time RT-PCR for the detection of enteroviruses in water samples originating from Marrakech city, and on the other hand to show that tap water of this city does not present any risk of contamination by this type of germ. © 2011 Academic Journals. Source
Bellete B.,Laboratoire Of Parasitologie Et Mycologie |
Raberin H.,Laboratoire Of Parasitologie Et Mycologie |
Flori P.,Laboratoire Of Parasitologie Et Mycologie |
El Akssi S.,Laboratoire Aliments |
And 4 more authors.
Natural Product Research | Year: 2012
The aim of the study was to determine the antifungal effects of the essential oil of Thymus broussonetii Boiss (EOT), an endemic plant in Morocco against Candida albicans, Aspergillus fumigatus and the dermatophytes. EOT was extracted by steam distillation. A suspension of up to 500μL of C. albicans at a concentration of 108CFUmL-1 and A. fumigatus at a concentration of 1010sporesmL-1 were inhibited by 20μL of EOT incorporated in tubes containing 4mL of Sabouraud broth. In Sabouraud-chloramphenicol agar slants containing different concentrations of essential oil, 5104 A. fumigatus spores were inhibited by 6L (0.0015mLmL-1) of the EOT. It has shown good anti-C. albicans and anti-A. fumigatus activity. All the dermatophytes tested were inhibited by 3L (0.00075mLmL1) of EOT; the latter has the potential to be a good alternative to the conventional antifungal drugs which are usually expensive and with high toxicity. © 2012 Copyright Taylor and Francis Group, LLC. Source
Dahbi A.,Laboratoire Aliments |
Bellete B.,Groupe Immunite des Muqueuses et Agents Pathogenes GIMAP |
Flori P.,Groupe Immunite des Muqueuses et Agents Pathogenes GIMAP |
Hssaine A.,Laboratoire Aliments |
And 5 more authors.
Parasitology Research | Year: 2010
In this study, we have evaluated the effect of essential oils of Thymus broussonetii Boiss, an endemic plant of Morocco in experimental transmission of Toxoplasma gondii cysts in mice. These oils were obtained by hydrodistillation and were administered to mice at 20 μg/animal orally at the time of infection and for several days thereafter. This resulted in total absence of intracerebral cysts in mice who received the essential oils signifying that these essential oils of thyme have a blocking effect on the appearance of the cysts. In addition, no abnormality was observed in the control mice who received the essential oils of thyme. © 2010 Springer-Verlag. Source