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Jokerst J.V.,University of Texas at Austin | Jacobson J.W.,Labnow Inc. | Bhagwandin B.D.,Labnow Inc. | Floriano P.N.,Rice University | And 2 more authors.
Analytical Chemistry | Year: 2010

There have been many recent advances in the nano-biochip analysis methodology with implications for a number of high-morbidity diseases including HIV, cancer, and heart disease. (To listen to a podcast about this article, please go to the Analytical Chemistry multimedia page at ancham/audio/index.html.) © 2010 American Chemical Society.

Miller C.S.,University of Kentucky | Foley J.D.,University of Kentucky | Bailey A.L.,University of Kentucky | Campell C.L.,University of Kentucky | And 10 more authors.
Biomarkers in Medicine | Year: 2010

Salivary diagnostics is an emerging field that has progressed through several important developments in the past decade, including the publication of the human salivary proteome and the infusion of federal funds to integrate nanotechnologies and microfluidic engineering concepts into developing compact point-of-care devices for rapid analysis of this secretion. In this article, we discuss some of these developments and their relevance to the prognosis, diagnosis and management of periodontitis, as an oral target, and cardiovascular disease, as a systemic example for the potential of these biodiagnostics. Our findings suggest that several biomarkers are associated with distinct biological stages of these diseases and demonstrate promise as practical biomarkers in identifying and managing periodontal disease, and acute myocardial infarction. The majority of these studies have progressed through biomarker discovery, with the identified molecules requiring more robust clinical studies to enable substantive validation for disease diagnosis. It is predicted that with continued advances in this field the use of a combination of biomarkers in multiplex panels is likely to yield accurate screening tools for these diagnoses in the near future. © 2010 Future Medicine Ltd.

Diaz M.R.,University of Miami | Jacobson J.W.,Labnow Inc. | Goodwin K.D.,National Oceanic and Atmospheric Administration | Dunbar S.A.,Luminex Corporation | Fell J.W.,University of Miami
Limnology and Oceanography: Methods | Year: 2010

Harmful algal blooms (HABs) are a serious public health risk in coastal waters. As the intensity and frequency of HABs continue to rise, new methods of detection are needed for reliable identification. Herein, we developed a high-throughput, multiplex, bead array technique for the detection of the dinoflagellates Karenia brevis and Karenia mikimotoi. The method combined the Luminex detection system with two novel technologies: locked nucleic acid-modified oligonucleotides (LNA) and Mirus Label IT® nucleic acid technology. To study the feasibility of the method, we evaluated the performance of modified and unmodified LNA probes with amplicon targets that were biotin labeled with two different strategies: direct chemical labeling (Mirus Label IT) versus enzymatic end-labeling (single biotinylated primer). The results illustrated that LNA probes hybridized to complementary single-stranded DNA with better affinity and displayed higher fluorescence intensities than unmodified oligonucleotide DNA probes. The latter effect was more pronounced when the assay was carried out at temperatures above 53°C degree. As opposed to the enzymatic 5′ terminal labeling technique, the chemicallabeling method enhanced the level of fluorescence by as much as ~83%. The detection limits of the assay, which were established with LNA probes and Mirus Label IT system, ranged from 0.05 to 46 copies of rRNA. This high-throughput method, which represents the first molecular detection strategy to integrate Luminex technology with LNA probes and Mirus Label IT, can be adapted for the detection of other HABs and is well suited for the monitoring of red tides at pre-blooming and blooming conditions. © 2010, by the American Society of Limnology and Oceanography.

Labnow Inc. | Date: 2010-03-09

Electro-mechanical system, consisting of one or more plastic encapsulated biochip cartridges and a compatible optical reader that jointly process and visually display chemical reactions between programmed reagents and organic fluid samples to identify the presence of certain target cells or properties.

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