Havana, Cuba
Havana, Cuba

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Gonzalez A.,Institute of Tropical Medicine | Rodriguez G.,LABIOFAM | Bruzon R.Y.,Institute of Tropical Medicine | Diaz M.,Institute of Tropical Medicine | And 3 more authors.
Journal of Vector Ecology | Year: 2013

The use of insect pathogens is a viable alternative for insect control because of their relative specificity and lower environmental impact. The search for wild strains against dipterans could have an impact on mosquito control programs. We have made an extensive screening of soil in western Cuba to find bacteria with larvicidal activity against mosquitoes. A total of 150 soil samples were collected and isolates were identifying using the API 50 CHB gallery. Phenotypic characteristics were analyzed by hierarchical ascending classification. Quantitative bioassays were conducted under laboratory conditions following the World Health Organization protocol in order to ascertain the toxicity and efficacy of isolates. The protein profiles of the crystal components were determined by SDS-PAGE. Eight hundred and eighty-one bacterial isolates were obtained, and 13 isolates with entomopathogenic activity were isolated from nine samples. Nine isolates displayed higher entomopathogenic activity against both Cx. quinquefasciatus and Ae. aegypti compared with the reference strain 266/2. All toxic isolates showed higher biological potency than the 266/2 strain. These isolates with high entomopathogenic activity displayed a protein pattern similar to the B. thuringiensis var. israelensis IPS-82 and 266/2 strains. These results are a valuable tool for the control of Diptera of medical importance. © 2013 The Society for Vector Ecology.

Suarez J.,University of Matanzas | Martin G.,University of Matanzas | Sotolongo J.,LABIOFAM | Savran V.,Direccion de Planificacion Fisica | And 2 more authors.
Cuban Journal of Agricultural Science | Year: 2014

Since 2009 the International Project BIOMAS-CUBA is being executed. This is focused on biomass use as renewable energy source, on contributing to improve living conditions and attaining the coexistence between mitigation and adaptation to climate change, feeding security and energetic sustainability in rural areas. BIOMAS-CUBA covers the production and utilization of biodiesel and biogas, biomass gasification and the production of bioproducts. The project is directed at the integrated production of foods and energy in the context of agroenergetic farms of agro-ecological basis, with the purpose of participating in local development. It promotes a local agricultural innovation model, with large involvement of producers and impact studies. Its phase 1 was executed in 87 scenarios of five Cuban provinces making possible the sowing of 109 ha of Jatropha curcas L. associated to cultures, the installation of a plant of biodiesel production (105 600 liters/year) and two biomass gasifiers for generating electricity, the construction of 69 biodigestors and the installation of 52 plants for the production of the IHplus bioproduct of wide spectrum of agricultural use -in nine provinces-, with 3.4 benefit/cost, included the investment. If phase 1 was centered on the promotion of agroenergetic farms, the II (2012-2015) is directed at the formulation and implementation of local strategies of integrated production of foods and energy in six municipalities, accompanying the governments and other actors, as well as to attain greater incidence in national, regional and local policies, associated with food security, renewable energies and environment.

Garcia-Valtanen P.,University Miguel Hernández | Del Mar Ortega-Villaizan M.,University Miguel Hernández | Martinez-Lopez A.,University Miguel Hernández | Medina-Gali R.,Labiofam | And 5 more authors.
Autophagy | Year: 2014

It has not been elucidated whether or not autophagy is induced by rhabdoviral G glycoproteins (G) in vertebrate organisms for which rhabdovirus infection is lethal. Our work provides the first evidence that both mammalian (vesicular stomatitis virus, VSV) and fish (viral hemorrhagic septicemia virus, VHSV, and spring viremia carp virus, SVCV) rhabdoviral Gs induce an autophagic antiviral program in vertebrate cell lines. The transcriptomic profiles obtained from zebrafish genetically immunized with either Gsvcv or Gvhsv suggest that autophagy is induced shortly after immunization and therefore, it may be an important component of the strong antiviral immune responses elicited by these viral proteins. Pepscan mapping of autophagy-inducing linear determinants of Gvhsv and Gvsv showed that peptides located in their fusion domains induce autophagy. Altogether these results suggest that strategies aimed at modulating autophagy could be used for the prevention and treatment of rhabdoviral infections such as rabies, which causes thousands of human deaths every year. © 2014 Landes Bioscience.

Curbelo A.,CETEX CENPALAB | Mancebo A.,CETEX CENPALAB | Molier T.,LABIOFAM | Arteaga M.E.,CETEX CENPALAB | And 12 more authors.
Toxicological and Environmental Chemistry | Year: 2011

Biological control agents have become a useful alternative for the reduction of the use of chemical insecticides. LABIOFAM (Cuba) is developing a new formulation of a biolarvicide that possesses as active biological agent Bacillus thuringiensis var israelensis serotype H14. In order to evaluate the genotoxicity of this new formulation, an in vivo battery test was used: micronucleus (MN), chromosome aberrations (CAs), and sperm morphology (SM) assays. A dose of 6.45×108 spores was administered per animal via oral administration. Bone marrow cells were collected 24 h after a two day treatment for the MN assay, and 24 h after a unique treatment for the CA assay, using cyclophosphamide as the positive control. Sperm cells were collected at 5 weeks from the first of five administrations for the SM test, using acrylamide as positive control. Bacillus thuringiensis var israelensis serotype H14 failed to show either a significative increase of micronucleated polychromatic erythrocytes, chromosomal aberrations, or sperm abnormalities. Acute oral administration of a high dose of Bacillus thuringiensis var israelensis serotype H14 did not produce mutagenic effects in bone marrow or sperm cells. © 2011 Taylor & Francis.

Vera M.A.,Institute Medicina Tropical Pedro Kouri IPK | Rodriguez A.G.,LABIOFAM | Morejon D.D.,Institute Medicina Tropical Pedro Kouri IPK | Diaz L.M.,Institute Medicina Tropical Pedro Kouri IPK | Tirado M.G.G.,Institute Medicina Tropical Pedro Kouri IPK
Revista Cubana de Medicina Tropical | Year: 2010

INTRODUCTION: The standard plaque reduction neutralization technique has been performed in BHK-21 cells for more than 20 years in Cuba to determine the neutralizing antibodies to dengue. At the end of 2007, the WHO implemented a program to harmonize this technique at all the laboratories worldwide. OBJECTIVES: the present study was aimed at standardizing the plaque-reduction neutralization technique in Vero cells from the Cultural Lab of "Pedro Kourí" Tropical Medicine Institute. METHODS: viral strains suggested by Who, strains from the Strain Bank of Arbovirus Laboratory, as well as the panel of human sera from dengue-suspected patients after 5 days of the symptoms. RESULTS: it was proved that suspension- cultured Vero cells allow higher plaque capacity and quality. Similar results were reached using Arbovirus Bank strains and WHO reference strains. CONCLUSIONS: it was possible to standardize the plaque-reduction neutralization in Vero cells, with results similar to those of BHK-21 but in longer length of time. Standardization of this technique will allow comparing the outcome of neutralization in the epide-miological studies and vaccine research with those of other laboratories worldwide.

PubMed | National Center for Laboratory Animals Breeding, LABIOFAM and University of Habana
Type: Journal Article | Journal: Regulatory toxicology and pharmacology : RTP | Year: 2014

Bacillus thuringiensis (Bt) is the best known and most widely used of all pesticidal microbes. The aim of this study was to assess the toxicity of a new formulation of Bacillus thuringiensis var israelensis SH-14 in rats through acute dermal toxicity, dermal and eye irritation experiments. The acute dermal toxicity and dermal and eye irritation studies were performed using rabbits according to the United States Environmental Protection Agency guidelines 885.3100, 870.2500 and 870.2500, respectively. The skin sensitization study was carried out in accordance to the EPA OPPTS 870.2600 using guinea pigs. There was no mortality and no evidence of treatment-related toxicity in acute dermal toxicity test. No dermal responses, including erythema/eschar or edema, were found in rabbits treated with the new formulation of Bti SH-14. Minimum response was observed after eye application of test substance. No skin sensitization reactions were observed after the challenge with the new formulation of Bti SH-14 in the Bti SH-14-treated guinea pigs. In summary, the present study demonstrated that the new formulation of Bti SH-14 is not acutely toxic via dermal route, has low eye irritation and would not cause dermal irritation or hypersensitivity to tested animals.

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