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Tunis, Tunisia

Ouneissa R.,Institute Pasteur Of Tunis | Bahri O.,Institute Pasteur Of Tunis | Ben Yahia A.,Institute Pasteur Of Tunis | Touzi H.,Institute Pasteur Of Tunis | And 3 more authors.
Hepatitis Monthly | Year: 2013

Background: Hepatitis B virus (HBV) infection is a public health problem in developing countries. HBV genotypes play major role in the evolution of infection since they were involved in different clinical presentations and response to treatment. Objectives: This study was conducted to evaluate the efficiency of restriction fragment length polymorphism (RFLP) analysis for HBV genotyping. Patients and Methods: We investigated 98 samples collected from patients chronically infected with HBV. HBV genotypes were determined by analysis of patterns obtained after amplification in Pre-S region and digestion of the amplicon by two endonucleases AvaII and DpnII. Obtained results were confirmed by partial sequencing in the same region. Results: Two different HBV genotypes were detected in this study, Genotype D (in 95. 9%) and Genotype A (in 4.1%). Seventy-four samples (75.5%) were successfully genotyped with RFLP analysis and all classified as genotype D. The remaining 24 samples (24.5%) which were un-genotyped by RFLP analysis, were classified by partial sequencing of the pre-S region as HBV genotype D (20 samples, 20.4%) and genotype A (4 samples, 4.1%). Atypical profiles were significantly associated with advanced liver disease (P = 0.001) as well as older age (P < 0.05). Conclusions: Several previous studies used PCR-RFLP to genotype HBV; however, we showed the high risk to obtain atypical profiles, especially in advanced stages of chronic infection, with as results difficulties to genotype the virus. These profiles resulted from the accumulation of mutations during natural course of infection resulting in a modification in restriction sites for enzymes. So, we recommended completing the investigation by partial sequencing to confirm obtained results. © 2013, Kowsar Corp.; Published by Kowsar Corp.

Dokmak S.,Beaujon Hospital | Ben Safta Y.,Hospital la Rabta | Fteriche F.S.,Beaujon Hospital | Aussilhou B.,Beaujon Hospital | Belghiti J.,Beaujon Hospital
Annals of Surgical Oncology | Year: 2014

A 54-year-old woman with multiple telangiectatic hepatocellular adenoma without degeneration (>5 cm) underwent laparoscopic right hepatectomy with the hanging maneuver. The patient was installed in the supine position with the legs spread apart. Five trocars were used for the intervention. The liver hanging maneuver was performed up-to-down with a nasogastric tube and no prior mobilization of the right liver. Surgery lasted 270 min with 100 ml of blood loss and 15 min of hepatic pedicle clamping. The patient was discharged on postoperative day 7. This anterior approach avoids difficult mobilization of the right liver, guides the anatomical transaction plane, limits bleeding, and increases the number of patients who can benefit from the laparoscopic approach. © 2014, Society of Surgical Oncology.

Mrabet D.,Hospital la Rabta | Rekik S.,Hospital la Rabta | Sahli H.,Hospital la Rabta | Meddeb N.,Hospital la Rabta | Sellami S.,Hospital la Rabta
Rheumatology International | Year: 2012

We report a new case of polyostotic fibrous dysplasia (FD). A 26-year-old woman was referred to our department complaining of pain in her left arm. She had suffered for tow fractures in left leg and arm previously. Plain radiographs showed osteolytics lesions at the left humerus and radius. Histological examination of the surgical specimens showed FD. She has beneficed with zoledronic acid perfusion. © Springer-Verlag 2011.

Sediri Y.,Research laboratory LR99ES11 | Kallel A.,Research laboratory LR99ES11 | Feki M.,Research laboratory LR99ES11 | Mourali S.,Hospital la Rabta | And 5 more authors.
European Journal of Internal Medicine | Year: 2011

Background: Apolipoproteins AI-CIII-AIV play important roles in the metabolism of triglycerides and high-density lipoprotein cholesterol. However, whether genetic variations in the ApoAI-CIII-AIV gene cluster are associated with the risk of myocardial infarction (MI) remains uncertain. In the present study, we examined a possible association of the ApoCIII SacI polymorphism in the ApoAI-CIII-AIV gene cluster with lipid parameters and MI in a sample of the Tunisian population. Methods: A total of 326 Tunisian patients with MI and 361 controls were included in the study. Genotypes were determined by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis. Results: A significant difference in genotype distribution and allele frequency was observed between patients and controls. At the multivariate analysis after adjustment for traditional vascular risk factors, the ApoCIII SacI polymorphism was significantly associated with MI, according to co-dominant and dominant models (co-dominant model odds ratio [OR]: 1.53, 95% confidence interval [CI]: 1.0-2.35, p = 0.04; dominant model OR: 2.02, 95% CI: 1.11-3.67, p = 0.02). The MI patient group showed a significant higher frequency of the S2 allele compared to the controls (10.2% vs. 6.5%; OR: 1.64, 95% CI: 1.10-2.47, p = 0.01). There was no statistically significant association between ApoAI-CIII-AIV cluster gene polymorphism and lipid, lipoprotein, and apolipoprotein levels in both MI patients and controls. Conclusion: In the current study, a significant association between the ApoCIII SacI polymorphism (presence of S2 allele) and MI in the Tunisian population was found. © 2011 European Federation of Internal Medicine.

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