Aizawa Y.,Niigata University |
Sato A.,Niigata University |
Watanabe H.,Niigata University |
Chinushi M.,Niigata University |
And 11 more authors.
Journal of the American College of Cardiology | Year: 2012
Objectives: This study evaluated the pause-dependency of the J-wave to characterize this phenomenon in idiopathic ventricular fibrillation (VF). Background: The J-wave can be found in apparently healthy subjects and in patients at risk for sudden cardiac death, and risk stratification is therefore needed. Methods: Forty patients with J-wave-associated idiopathic VF were studied for J waves with special reference concerning pause-dependent augmentation. J waves were defined as those <0.1 mV above the isoelectric line and were compared with 76 non-VF patients of comparable age and sex. Results: The J-wave was larger in patients with idiopathic VF than in the controls: 0.360 ± 0.181 mV versus 0.192 ± 0.064 mV (p = 0.0011). J waves were augmented during storms of VF (n = 9 [22.5%]), which was controlled by isoproterenol; they disappeared within weeks in 5 patients. In addition, sudden prolongation of the R-R interval was observed in 27 patients induced by benign arrhythmia, and 15 patients (55.6%) demonstrated pause-dependent augmentation (from 0.391 ± 0.126 mV to 0.549 ± 0.220 mV; p < 0.0001). In the other 12 experimental subjects and in the 76 control subjects, J waves remained unchanged. Pause-dependent augmentation of J waves was detected in 55.6% (sensitivity) but was specific (100%) in the patients with idiopathic VF with high positive (100%) and negative (86.4%) predictive values. Conclusions: Pause-dependent augmentation of J waves was confirmed in about one-half of the patients with idiopathic VF after sudden R-R prolongation. Such dynamicity of J waves was specific to idiopathic VF and may be used for risk stratification. © 2012 American College of Cardiology Foundation.
Amit G.,Case Western Reserve University |
Kikuchi K.,Case Western Reserve University |
Kikuchi K.,Kyushu Kosei Nenkin Hospital |
Greener I.D.,Case Western Reserve University |
And 4 more authors.
Circulation | Year: 2010
BACKGROUND-: Safety and efficacy limit currently available atrial fibrillation (AF) therapies. We hypothesized that atrial gene transfer would allow focal manipulation of atrial electrophysiology and, by eliminating reentry, would prevent AF. METHODS AND RESULTS-: In a porcine AF model, we compared control animals to animals receiving adenovirus that encoded KCNH2-G628S, a dominant negative mutant of the IKr potassium channel α-subunit (G628S animals). After epicardial atrial gene transfer and pacemaker implantation for burst atrial pacing, animals were evaluated daily for cardiac rhythm. Electrophysiological and molecular studies were performed at baseline and when animals were euthanized on either postoperative day 7 or 21. By day 10, none of the control animals and all of the G628S animals were in sinus rhythm. After day 10, the percentage of G628S animals in sinus rhythm gradually declined until all animals were in AF by day 21. The relative risk of AF throughout the study was 0.44 (95% confidence interval 0.33 to 0.59, P<0.01) among the G628S group versus controls. Atrial monophasic action potential was considerably longer in G628S animals than in controls at day 7, and KCNH2 protein levels were 61% higher in the G628S group than in control animals (P<0.01). Loss of gene expression at day 21 correlated with loss of action potential prolongation and therapeutic efficacy. CONCLUSIONS-: Gene therapy with KCNH2-G628S eliminated AF by prolonging atrial action potential duration. The effect duration correlated with transgene expression. © 2010 American Heart Association, Inc.
Odate S.,Kyushu University |
Nakamura K.,Kyushu University |
Onishi H.,Kyushu University |
Kojima M.,Red Cross |
And 5 more authors.
Lung Cancer | Year: 2013
Tropomyosin-related kinase B (TrkB) plays an important role in tumor progression in various kinds of cancers; however, little is known about biological significance of TrkB in human lung cancer, especially large cell neuroendocrine carcinoma (LCNEC). We hereby investigated the expressions of TrkB and its ligand brain-derived neurotrophic factor (BDNF) in clinical specimens and their influences on phenotypes of invasiveness and tumorigenicity for LCNEC. The expressions of TrkB and BDNF analyzed by immunohistochemistry for patients samples with lung cancer (n=104) were significantly higher in neuroendocrine tumor (NET) compared with non-NET. In particular, LCNEC, a subtype of NET, exhibited significantly higher TrkB and BDNF expressions than another NET type: small cell lung cancer (SCLC), and a significant correlation between TrkB and BDNF expressions was noted in LCNEC but not in SCLC. In vitro assay, exogenous BDNF addition enhanced the invasion into matrigels of LCNEC cells, whereas inhibition of TrkB or BDNF suppressed matrix metalloproteinase-2 and -9 activities and the invasiveness. Exogenous BDNF also increased anchor-independent colony formation on soft agar gels for LCNEC, while inhibition of TrkB or BDNF suppressed the anchorage-independency. In vivo experiments, implanted LCNEC cells pretreated with TrkB-siRNA developed no subcutaneous tumor in all six nude mice, although those with control-siRNA formed tumors in four of six nude mice. In conclusion, BDNF/TrkB signal is involved in malignant progression of invasiveness and tumorigenicity for LCNEC, and may be a potential target for LCNEC without standard therapy. © 2012 Elsevier Ireland Ltd.
Kayashima K.,Kyushu Kosei Nenkin Hospital |
Sozen R.,Nishinihon Occupational Health Service Center |
Okura D.,Kyushu Kosei Nenkin Hospital
Japanese Journal of Anesthesiology | Year: 2014
A 32-year-old pregnant woman (height 162 cm, weight 86 kg, age of fetus 25 weeks) without preoperative complications underwent an emergent cesarean section under general anesthesia. She was intubated with a 7.0-mm tracheal tube 40 s after receiving rocuronium 0.93 mg·kg-1 and thiamilal 375 mg. Anesthesia was maintained with oxygen, air, sevoflurane 1.0-2.5%, and fentanyl 425 μg. Nine minutes after the end of surgery (operation time 71 minutes), a train-of-four count of 2 with 20 spontaneous breaths was noted, and sugammadex 2.3 mg·kg-1 was administered. However, the train-of-four count was 3 even five minutes after sugammadex administration. Fifteen minutes after sugammadex administration (train-of-four ratio 14%), she received atropine 0.5 mg and neostigmine 1.0 mg. Ten minutes later, the train-of-four ratio increased to 89%, and the patient was successfully extubated with no respiratory suppression. We speculate that the rocuronium dose (0.93 mg·kg -1) was too high in this obese patient and sugammadex dose at the end of the surgery was not enough for reversal of rocuronium-induced neuromuscular blockade.
Nishida Y.,Kyushu Kosei Nenkin Hospital
[Rinshō ketsueki] The Japanese journal of clinical hematology | Year: 2013
A 68-year-old man was admitted to our hospital because of left back pain and systemic lymphadenopathy with hypercalcemia. Serum anti-HTLV-1 antibody was positive. Left cervical lymph node (LN) biopsy revealed proliferation of medium-sized to large CD4-positive atypical cells with modest infiltration of CD20 and Epstein-Barr virus (EBV)-encoded RNA dual-positive atypical large cells. Monoclonal integration of HTLV-1 proviral DNA, plus clonal rearrangement of the T-cell receptor chain gene and the immunoglobulin heavy chain gene, were detected in the same LN specimen. Composite lymphoma consisting of adult T-cell leukemia/lymphoma (ATL) and EBV positive diffuse large B-cell lymphoma (DLBCL) was diagnosed. He was successfully treated with aggressive chemotherapy including rituximab and attained remission. However, eight months later, he developed right shoulder pain due to multiple bone invasions with bilateral cervical lymphadenopathy. Biopsies of a bone lesion and cervical LN revealed recurrence of ATL alone. The patient died despite salvage chemoradiotherapy. These findings suggest that ATL-related immunodeficiency might induce EBV-associated DLBCL.
Yamamoto U.,Kyushu Kosei Nenkin Hospital |
Mohri M.,Kyushu Kosei Nenkin Hospital
Experimental and Clinical Cardiology | Year: 2010
BACKGROUND: Renal insufficiency, a common condition among patients with chronic heart failure, complicates the management of heart failure. However, the influence of renal insufficiency on sleep-disordered breathing (SDB) - another important comorbidity of heart failure - has not been well studied. METHODS: Seventy-nine patients (60 men and 19 women) with stable, symptomatic heart failure caused by left ventricular systolic dysfunction (left ventricular ejection fraction of less than 45%) were studied. RESUlTS: Thirty-nine patients (49%) had SDB as defined by an apnea-hypopnea index (AHI) of five or greater: 15 patients were classified as having mild SDB (AHI of five or greater and less than 15), 10 patients as having moderate SDB (AHI of 15 or greater and less than 30) and 14 patients as having severe SDB (AHI of 30 or greater). The etiology of SDB was predominantly central. Plasma brain natriuretic peptide concentration in the severe SDB group was 587±377 pg/mL, which was significantly higher than those of the remaining three groups (P<0.05). On the other hand, estimated glomerular filtration rate (eGFR) was comparable between non-SDB and SDB groups. There was no statistically significant correlation between eGFR and AHI, or between eGFR and the number of central sleep apneas in the study patients. CONCLUSION: Higher plasma brain natriuretic peptide concentrations were associated with more severe SDB, whereas the level of eGFR was not correlated with the severity of SDB. The results suggest that renal dysfunction plays a relatively minor role in determining breathing abnormalities in chronic heart failure. ©2010 Pulsus Group Inc. All rights reserved.
Mohri M.,Kyushu Kosei Nenkin Hospital |
Motohama R.,Kyushu Kosei Nenkin Hospital |
Sato N.,Kyushu Kosei Nenkin Hospital
Acta Cardiologica | Year: 2013
Objective Red cell distribution width (RDW) is a measure of variation in the volume of circulating erythrocytes and has recently emerged as a powerful prognostic marker in heart failure. We studied the eff ect of 5-month, home-based cardiac rehabilitation on RDW. Methods and results Sixty-two patients (age: median 67 years, IQR 63-74) with chronic heart failure caused by reduced left ventricular systolic function were enrolled. Exercise time and physical activity levels out of the hospital were determined by a single-axial accelerometer. Baseline RDW values were: median 13.6, IQR 12.8-14.4%, with 13 patients (21.0%) showing values above the upper normal limit. RDW values were decreased signifi cantly after training in the 13 patients with elevated baseline RDW values (P < 0.01), but not in the remaining 49 patients with normal baseline RDW values. There was a highly signifi cant correlation between baseline RDW and changes in RDW after exercise training (P = 0.0001, r2= 0.628, n = 62). The time spent for moderate (> 3 METs) exercise was: median 9.0, IQR 3.0-18.8 minutes per day. Conclusions Relatively low intensity, home-based cardiac rehabilitation was associated with decreased RDW in heart failure patients with abnormal RDW.
Nagao Y.,University of Occupational and Environmental Health Japan |
Hisaoka M.,University of Occupational and Environmental Health Japan |
Matsuyama A.,University of Occupational and Environmental Health Japan |
Kanemitsu S.,University of Occupational and Environmental Health Japan |
And 7 more authors.
Modern Pathology | Year: 2012
Since the discovery of small non-coding RNAs, the analyses of microRNA (miRNA) expression patterns in human cancer have provided new insights into cancer biology. miRNA-21 has been suggested to be one of the miRNAs that have an important role in the development or biological behavior of a variety of malignancies, including pancreatic cancer. This study was conducted to evaluate the relationship between the expression of miRNA-21 and that of its molecular targets, programmed cell death 4 (PDCD4) and tissue inhibitor of metalloproteinase (TIMP3), in pancreatic ductal adenocarcinoma. The study included 65 pancreatic ductal adenocarcinomas and 5 normal pancreatic tissue specimens for comparison. The miRNA expression profiling of five selected pancreatic ductal adenocarcinomas and five normal pancreatic specimens was performed using a microarray platform, and was evaluated by a hierarchical clustering analysis. The miRNA most highly expressed in pancreatic ductal adenocarcinomas (ie, miRNA-21) was further assessed by quantitative real-time reverse transcription PCR (RT-PCR) assays in the 65 pancreatic ductal adenocarcinoma cases. The expression pattern of its molecular targets (eg, PDCD4 and TIMP3) in pancreatic ductal adenocarcinoma was examined immunohistochemically. In the microarray analyses, 28 miRNAs were upregulated in pancreatic ductal adenocarcinoma compared with normal pancreatic tissue, whereas 48 miRNAs were downregulated. miRNA-21 was the most significantly overexpressed miRNA in the pancreatic ductal adenocarcinomas analyzed, and was also highly expressed in 75% of the 65 pancreatic ductal adenocarcinomas examined by real-time RT-PCR. High miRNA-21 expression was correlated with a worse prognosis in the pancreatic ductal adenocarcinoma patients (P=0.045). The immunohistochemical expression patterns of PDCD4 (reduced nuclear staining pattern) and TIMP3 (downregulated expression) were significantly associated with both the upregulated miR-21 expression (P<0.05) and the poor survival of the patients (P<0.001 and P=0.001, respectively). Our data suggest that an overexpression of miRNA-21 is, therefore, associated with the biological behavior of pancreatic ductal adenocarcinoma via the downregulation of the expression of tumor suppressors, PDCD4 and TIMP3, thus resulting in tumor progression and the adverse clinical course of pancreatic ductal adenocarcinoma. © 2012 USCAP, Inc. All rights reserved.
Hara T.,Kyushu Kosei Nenkin Hospital
The Journal of arthroplasty | Year: 2012
We performed cementless total hip arthroplasty combined with acetabular reconstruction with Chiari pelvic osteotomy on 3 hips in 2 patients. This procedure increased the osseous coverage of the shell and decreased the load around the hip by medialization of the hip center. Osteosynthesis of the proximal and distal bone fragment was performed using a shell with screws. Nonunion between the proximal and distal fragments was not noticed to include the case with small contact area. Postoperative dislocation had not occurred in these cases as of 1 year after surgery. This method may be an effective solution for severe dysplasia and revision surgery. Copyright © 2012 Elsevier Inc. All rights reserved.
Yamamoto U.,Kyushu Kosei Nenkin Hospital
BMJ case reports | Year: 2013
A 49-year-old man with fever, pain in both legs, purpuras and cyanosis was admitted to hospital. He was a heavy drinker, but did not have diabetes or other immunosuppressive disease. On admission, he was in shock, with haematological findings suggestive of disseminated intravascular coagulation, and liver and kidney failure. The presence of a scratch wound on his face caused by a cat, and linear, Gram-negative rods phagocytosed by polynuclear leucocytes on peripheral blood smear suggested Capnocytophaga canimorsus infection. On day 1, antibiotics (ampicillin/sulbactam) and catecholamines were initiated. The patient required haemodialysis three times per week for 3 weeks. His toes became necrotic but improved and amputation was not necessary. On day 52, he was discharged from hospital with only mild sensory impairment of the legs.