Kim N.-H.,Kyung Hee East West Pharmaceutical Research Institute |
Jeong J.-S.,Kyung Hee East West Pharmaceutical Research Institute |
Kwon H.-J.,Kyung Hee University |
Lee Y.-M.,Chungbuk National University |
And 3 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2010
We developed a simultaneous diagnostic method for phenylketonuria (PKU) and galactosemia through simultaneous determination of phenylalanine (Phe) and galactose (Gal) by high-performance liquid chromatography (HPLC) with pulsed amperometric detection (PAD). The intra- and inter-day precisions were <5.8%, with satisfactory mean recoveries (98.2-105%). For all PKU-positive samples, Phe levels were above the cut-off value (>30.0 mg/L), but Gal levels were nearly zero. For 77% of galactosemia-positive samples, Phe levels were above the cut-off value, but Gal levels were above the cut-off value (>80.0 mg/L) for all samples. Our HPLC-PAD method can reduce the false-positive rate of misdiagnosis for PKU and galactosemia. © 2010 Elsevier B.V. All rights reserved.
Jeon S.J.,Kyung Hee East West Pharmaceutical Research Institute |
Park H.J.,Kyung Hee University |
Gao Q.,Kyung Hee East West Pharmaceutical Research Institute |
Gao Q.,Kyung Hee University |
And 11 more authors.
Behavioural Brain Research | Year: 2015
Sleep loss, insomnia, is considered a sign of imbalance of physiological rhythm, which can be used as pre-clinic diagnosis of various neuropsychiatric disorders. The aim of the present study is to understand the pharmacological actions of α- or β-amyrin, natural triterpene compound, on the sleep in mice. To analyze the sleeping behavior, we used the well-known pentobarbital-induced sleeping model after single administration of either α- or β-amyrin. The sleeping onset time was remarkably decreased and duration was prolonged by β-amyrin (1, 3, or 10mg/kg) but not by α-amyrin (1, 3, or 10mg/kg). These effects were significantly blocked by GABAA receptor antagonist, bicuculline. Moreover, β-amyrin increased brain GABA level compared to the vehicle administration. Overall, the present study suggests that β-amyrin would enhance the total sleeping behavior in pentobarbital-induced sleeping model via the activation of GABAergic neurotransmitter system through GABA content in the brain. © 2015 Elsevier B.V.
Moon J.T.,Korea Basic Science Institute |
Jeon J.Y.,Korea Basic Science Institute |
Park H.A.,Korea Basic Science Institute |
Noh Y.-S.,Kyung Hee East West Pharmaceutical Research Institute |
And 4 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2010
3,4-Diphenyl-substituted 1H-furan-2,5-dione and 1H-pyrrole-2,5-dione derivatives were synthesized and evaluated for the inhibitory activities on LPS-induced PGE2 production in RAW 264.7 macrophage cells. Both 1H-furan-2,5-dione and 1H-pyrrole-2,5-dione rings as main scaffolds were easily obtained using one of three synthetic methods. Among the compounds investigated, 1H-3-(4-sulfamoylphenyl)-4-phenyl-pyrrole-2,5-dione (6l) showed a strong inhibitory activity (IC50 = 0.61 μM) of PGE2 production. © 2009 Elsevier Ltd. All rights reserved.