Gupta V.K.,Indian Institute of Technology Roorkee |
Gupta V.K.,Dr adh University Faizabad |
Yola M.L.,Dr adh University Faizabad |
Yola M.L.,Sinop University |
And 6 more authors.
Sensors and Actuators, B: Chemical | Year: 2013
A highly sensitive method for detection of DNA hybridization was developed. This method was based on the modification of glassy carbon electrode with gold nanoparticles (AuNPs) involving p-aminothiophenol (ATP) functionalized graphene oxide (GO). This GO was used as a platform for impedimetric genosensing using 5′-TA GGG CCA CTT GGA CCT-(CH2)3-SH-3′ single-stranded probe (ss-DNA), 5′-AGG TCC AAG TGG CCC TA-3′ (target DNA), 5′-SH-C6-TAG GGC CA-3′ (non-complementary-1) and 5′-SH-C6-TGC CCG TTA CG 3-′ (non-complementary-2) oligonucleotide sequences. The film exhibited excellent properties for immobilizing DNA probes and sensing DNA hybridization. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), and found that the charge transfer resistance (Rct) of the electrode increased with the concentration of the target DNA hybridized with the ss-DNA. The linear detection range was from 1.0 × 10-13 M to 1.0 × 10-7 M and the detection limit was 1.10 × 10-14 M (n = 6). Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability, and high sensitivity. © 2013 Elsevier B.V.
Akcayir M.,Kirikkale University |
Dundar H.,Kyrgyzstan-Turkey Manas University |
Akcayir G.,Gazi University
Computers in Human Behavior | Year: 2016
The purpose of this study is to determine levels of technological proficiency among university students to see if they should be characterized as "digital natives." For this purpose, rather than using types and frequency of technology use to characterize a digital native, the Digital Native Assessment Scale (DNAS) developed by Teo (2013) was employed. This scale has an established reliability and validity measure. It measures several factors (e.g., grew up with technology, comfortable with multi-tasking, reliant on graphics for communication, thrive on instant gratifications and rewards, etc.) relating to accepted characteristics of a digital native. The participants in the study were 560 university students, 278 of whom were from Turkey and 282 from Kyrgyzstan. The findings indicate that these university students actively used computers and the Internet. The statistical results show that digital natives can be characterized by including their academic year (Freshman, Sophomore, etc.), national culture, and experiences with technology (computers, tablet PCs, and the Internet). The results also indicate that there are no significant differences in the participants' perceptions of themselves as digital natives due to their gender or academic disciplines. © 2016 Elsevier Ltd. All rights reserved.
Yola M.L.,Sinop University |
Yola M.L.,Hacettepe University |
Atar N.,Dumlupinar University |
Qureshi M.S.,Ankara University |
And 3 more authors.
Sensors and Actuators, B: Chemical | Year: 2012
This study reports the electrochemical modification of glassy carbon (GC) surface with etodolac (ETO), the characterization of this ETO modified glassy carbon (ETO/GC) electrode and its electroanalytical application for the determination of Pb(II) ions. Binding type of the etodolac with the glassy carbon surface was investigated and found that it was through the etheric linkage revealed from the reflection-absorption infrared spectroscopy (RAIRS). The ETO/GC electrode was characterized by cyclic voltammetry (CV), reflection-adsorption IR spectroscopy, electrochemical impedance spectroscopy (EIS), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The ETO/GC electrode was found to be suitable for selective determination of Pb(II) by adsorptive stripping voltammetry in the solutions of the mixture of heavy metal ions, such as Cu(II) and Cd(II) and showed high stability and reproducibility. The stability and the potential range of the ETO/GC electrode were also studied. The developed method was validated according to the ICH guideline and found to be linear, sensitive, selective, precise and accurate. The linearity range of Pb(II) was 5.0 × 10 -10 to 1.0 × 10 -8 M with the detection limit (S/N = 3) of 1.67 × 10 -10 M. The validated method was applied successfully for the determination of Pb(II) in real samples. © 2012 Elsevier B.V. All rights reserved.
Caglayan T.,Kyrgyzstan-Turkey Manas University |
Kirikci K.,Selcuk University |
Aygun A.,Selcuk University
Journal of Applied Poultry Research | Year: 2014
The current research was conducted to determine the comparison of hatchability and some egg quality characteristics in spotted and unspotted partridge (Alectoris chukar) eggs. The research was carried out on 51 male and 102 female chukar partridges (Alectoris chukar) aged 50 wk, which were raised at the Research and Application Farm of the Faculty of Veterinary Medicine of Selcuk University. In total, 244 spotted and 261 unspotted eggs were used in the research. The hatching eggs were stored at 75% RH and 13°C for 14 d. Thirty spotted and 30 unspotted eggs laid on the same day were used for the evaluation of egg quality characteristics. Egg weight, chick weight, egg weight loss during the hatching period, shape index, hatchability, and some egg quality characteristics were evaluated at the end of the research. Spotted eggs exhibited a higher fertility and hatchability, as well as a lower percentage egg weight loss and embryonic mortality when compared with unspotted eggs. No significant differences were observed between spotted and unspotted eggs for egg weight, chick weight, shape index, specific gravity, albumen height, Haugh unit, yolk color green-red chromaticity (a*), albumen pH, and yolk pH. Spotted eggs had higher eggshell color a*, eggshell color blue-yellow chromaticity (b*), and eggshell strength, as well as a lower eggshell color light-dark chromaticity (L*), yolk color L*, and yolk color b* scores when compared with unspotted eggs. It was demonstrated that spots on the eggshells of chukar partridges (Alectoris chukar) improved their hatchability by affecting various quality characteristics of their eggs. It was concluded that spotted eggs are more suitable for hatching. © 2014 Poultry Science Association Inc.
Cetintas H.,Kyrgyzstan-Turkey Manas University |
Cetintas H.,Balikesir UniversityBalikesir
Energy Sources, Part B: Economics, Planning and Policy | Year: 2016
This study investigates the causality relationship between energy consumption and economic growth in 17 transition countries, which are Albania, Armenia, Belarus, Bulgaria, Czech Republic, Estonia, Hungary, Kazakhstan, Kyrgyz Republic, Latvia, Moldova, Poland, Romania, Russian Federation, Slovenia, Ukraine, and Georgia. Empirical findings indicate that there is unidirectional causality from economic growth to energy consumption in the long run. The results support for conservation hypothesis suggests that energy conservation policies have no effect on economic growth. They can simultaneously achieve policy goals concerning growth and energy. © 2016 Taylor & Francis Group, LLC.
Ilicali C.,Kyrgyzstan-Turkey Manas University |
Icier F.,Ege University
Journal of Food Engineering | Year: 2010
Dehydrofreezing which is the drying of foods to intermediate moisture content and subsequent freezing has the advantages of lowering the transportation costs due to reduced weight and improved texture. The available empirical equations for freezing time prediction and the experimental data on thermo-physical properties are for fresh produce. Some of these empirical equations were used to predict the freezing times of papaya puree infinite cylinders with initial moisture contents ∼52% to ∼91%. The accuracies of these methods to predict the freezing times for final center temperatures of-10 °C and-18 °C were discussed. The most accurate methods for fresh and partially dries papaya puree were suggested. © 2010 Elsevier B.V. All rights reserved.
Ertas N.,Erciyes University |
Gonulalan Z.,Kyrgyzstan-Turkey Manas University |
Yildirim Y.,Erciyes University |
Karadal F.,Erciyes University
Food Control | Year: 2011
The aim of this study was to examine the presence of aflatoxin M1 (AFM1) in dairy product samples by enzyme-linked immunosorbent assay (ELISA). Aflatoxin M1 were found in 135 (64%) out of 210 analyzed samples. Only 7% of dairy product samples were contaminated at levels above the legal limits. AFM1 was detected in 43 (86%) of milk samples (ranging from 1 to 30 ng/l), in 38 (63%) of cheese (ranging from 12 to 378 ng/kg), in 28 (56%) of yoghurt (ranging from 2.5 to 78 ng/kg) and in 26 (52%) of dairy dessert samples (ranging from 1.5 to 80 ng/kg). None of the raw milk samples contained levels above the Turkish legal limit. The AFM1 levels of 7(14%) yoghurt, 5 (10%) dairy dessert and 3 (5%) cheese samples were above the limits of the Turkish Food Codex. It is emphasized that the presence of AFM1 in dairy products may be regarded as a potential hazard for human health. © 2011 Elsevier Ltd.
Atmaca H.T.,Kirikkale University |
Gazyagci A.N.,Kirikkale University |
Canpolat S.,Kirikkale University |
Kul O.,Kirikkale University |
Kul O.,Kyrgyzstan-Turkey Manas University
Parasites and Vectors | Year: 2013
Background: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. Methods. Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. The mice were sacrificed in groups of 5 at 2, 4, and 6 days after inoculation. Another group of 5 mice were used as the controls. Anti-glial fibrillary acidic protein (GFAP) and anti-T. gondii antibodies were used to compare GFAP-immunoreactive cells and anti-T. gondii-immunopositive areas in the liver between the T. gondii-infected groups and the healthy controls, respectively. Results: There was a significant correlation between the numbers of GFAP-positive hepatic stellate cells (HSCs) when they were compared with T. gondii antigen immunostaining (p < 0.05). The amount of T. gondii immunostaining increased significantly with the increase in the number of HSCs. Conclusions: There is a significant relationship between the number of HSCs and T. gondii antigens, which may represent an active role of HSCs in liver pathology and the pathobiology of T. gondii-related hepatitis. © 2013 Atmaca et al.; licensee BioMed Central Ltd.
Donmez N.,Kyrgyzstan-Turkey Manas University
TheScientificWorldJournal | Year: 2012
The objective of the present study was to evaluate the toxic effects of aflatoxin on some hematological parameters and to determine the preventive effectiveness of added glucomannan. In the study, 32 Merino rams were used, and the rams were separated equally to four groups as control (C), glucomannan (G), glucomannan + aflatoxin (AG), and aflatoxin (A). Erythrocyte, leukocyte count, hemoglobin, and hematocrit levels were decreased in A group compared with the other groups, and there was a reduction in similar parameters in AG group compared to control values. On the other hand, these parameters were tended to increase in AG group compared to A group values. Aflatoxicosis caused the lymphocytopenia and monocytopenia but increased percentage of neutrophil counts. In conclusion, the results determined in the study might be important to demonstrate the effects of aflatoxicosis and glucomannan on some haematological parameters before the clinical symptoms appear.
Canbulat S.,Kyrgyzstan-Turkey Manas University |
Canbulat S.,Kastamonu University
Turkish Journal of Zoology | Year: 2015
An updated checklist of the species of Turkish Raphidioptera and further analysis concerning their zoogeographical distribution are presented in this study. The present checklist has been based on studies from the literature and includes records from extensive material that I have identified over the last few years. The Turkish Raphidioptera presently comprises 40 species in 8 genera and 2 families. There are 38 species of Raphidiidae, while there are only 2 species of Inocelliidae in Turkey. Among these species, 19 species are recorded as endemic and the endemism rate is 47.5%. Distributions of these species within Turkey are given according to data provided from 81 provinces of the country. Three species have been known only from the type locality, whereas the other species have been recorded from various parts of the country. Analyses of the species of Turkish snakeflies were based on their origins. The faunal assemblage is represented by 28 taxa from Mediterranean, 8 species from Pontocaspian, 2 species from Syrian, 1 species from Cyprio-Syrian, and 1 species from Euro-Siberian elements in the Turkish fauna. Mediterranean subregion species are the most dominant constituents representing characteristic features of the fauna (70%), and 8 species are widely distributed in the Pontocaspian subregion (20%). The elements of the Mediterranean subregion of Turkey’s fauna are represented by 4 groups: 20 taxa from Anatolopontomediterranean, 6 species from Balkanopontomediterranean, and 2 species from Pontomediterranean faunal elements. Taxa are arranged hierarchically under the categories of family, genus, subgenus, and species. The addition of examined material turns this manuscript into an original contribution on the Turkish fauna, with new records of species of Raphidioptera in some provinces. The investigated specimens, of 40 in total, belong to 15 species. First records are 16, concerning 9 species and 11 provinces. © TÜBİTAK.