Kyoto Seibo College

Kyoto, Japan

Kyoto Seibo College

Kyoto, Japan
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Ohara K.,Kobe University | Ohara K.,Japan Society for the Promotion of Science | Kato Y.,Kobe University | Mase T.,Kobe University | And 7 more authors.
Eating and Weight Disorders | Year: 2014

Purpose: We investigated the relationship between eating behavior measured by the Dutch Eating Behaviour Questionnaire (DEBQ) and perception of body shape, examining the current physical status and ‘ideal’ physical parameters in females and males.Methods: The participants, 548 Japanese university students (age 19.2 ± 0.9 years, mean ± SD; 252 males, 296 females), completed a questionnaire which asked for their current physical status (e.g., weight and height), their ideal physical parameters, their perception of their current body shape, their ideal body shape, and their eating behaviors.Results: The ideal weight and ideal body mass index (BMI) were significantly higher than the current weight and BMI in the males, but significantly lower in the females. Among the females, the ideal body shape was smaller than their perception of current body shape. The DEBQ scores for restrained, emotional, and external eating were higher in the females than the males among the normal-weight participants, and among the underweight participants, the restrained eating and external eating scores were higher in the females than the males. Restrained eating was negatively associated with the discrepancy between the current and ideal weight, BMI, and body shape in both the males and females. Emotional eating was negatively associated with the discrepancy in current/ideal BMI and body shape only in the females.Conclusions: At least in Japanese university students, the gender differences in ideal body shape are related to eating behavior. © 2014, The Author(s).


Mase T.,Kyoto Seibo College | Ohara K.,Kobe University | Ohara K.,Japan Society for the Promotion of Science | Miyawaki C.,Heian Jogakuin St Agnes College | And 2 more authors.
International Journal of Women's Health | Year: 2015

Purpose: The present study investigated the infuence of peers’ and family members’ body shapes on the perception of body image and desire for thinness in Japanese female students. Methods: The study included 342 female, Japanese university students between the ages of 18 years and 22 years. They completed an anonymous questionnaire, which included questions related to anthropometry and body perception. Eating behavior was assessed by the Japanese version of the Eating Attitude Test-26. Results: Many students overestimated their body shape (81.2% of underweight students and 74.6% of normal students) and had a desire for thinness (41.0% of underweight students, 88.2% of normal students, and 100% of overweight students). One of the main reasons for the overes-timation of their body shape was comparison with others. Participants who were interested in a friend’s body shape were almost three times more likely to have a desire for thinness than those who were not interested in a female friend’s body shape (odds ratio: 3.06, P=0.014). Conclusion: The results indicate a possibility that a female Japanese student’s young female friends’ body shapes, infuences her desire for thinness or her perception of her own body shape. © 2015 Mase et al.


PubMed | Heian Jogakuin St Agnes College, Kyoto Seibo College, University of Shizuoka, Kobe University and Kinki University
Type: | Journal: BMC women's health | Year: 2015

Menstrual cycle-related symptoms are an important health issue for many women, and some may affect cardiac autonomic regulation. In the present study, we evaluated the cardiovascular and physiological stress response to 12-h short-term fasting in the menstrual phases of healthy young women.We performed a randomized crossover study. Subjects were seven female university students (age: 22.31.0 years). The experiments comprised four sessions: meal intake in the follicular phase, meal intake in the luteal phase, fasting in the follicular phase, and fasting in the luteal phase. All subjects participated in a total of four experimental sessions during two successive phases (follicular and luteal phase in the same menstrual cycle, or luteal phase and follicular phase in the next menstrual cycle) according to a randomized crossover design. R-R intervals were continuously recorded before and after meals, and power spectral analysis of heart rate variability was performed. Other physiological data were obtained before and 20, 40, 60, and 80 min after meal intake or after the corresponding time point of meal intake (fasting in the follicular or luteal phase).Heart rate decreased during fasting in the follicular and luteal phases. High frequency power increased during fasting in the follicular and luteal phases. In addition, salivary cortisol concentrations decreased during fasting in the luteal phase.In the present study, short-term fasting resulted in higher parasympathetic activity and lower cortisol levels in the luteal phase in these young women. These results indicate a possibility to produce an anti-stress effect in the luteal phase, which may reduce menstrual symptoms.


Tanaka M.,Kyoto Seibo College | Tanaka C.,J. F. Oberlin University | Kitagawa K.,Chukyo University
Japanese Journal of Physical Fitness and Sports Medicine | Year: 2015

This study examined the physiological response to different water depths in recreational synchronized swimming. Nine middle-aged, female, recreational swimmers carried out the same team free routines in deep water (deep-water synchronized swimming: DWS) and shallow water (shallow-water synchronized swimming: SWS). Heart rate (HR) was measured continuously during each performance, combined with estimation of metabolic equivalent (MET) values using individual linear regression equations of HR-oxygen uptake. These equations were created using the results of 12 water activities. Blood lactate concentration and systolic blood pressure were measured at the 1/3 and 2/3 stages and immediately after each performance period. HR and estimated METs during DWS (mean±SD, 152.9±7.5 beats·min-1 and 7.3±1.2) were significantly higher than those measured during SWS (131.8±11.3 beats·min-1 and 5.7±0.8). Blood lactate concentration and systolic blood pressure measured immediately after DWS were significantly higher than those measured after SWS. With DWS, blood lactate concentration at the 2/3 stage and immediately after the performance were significantly higher than those measured at the 1/3 stage, whereas in SWS no significant difference was found in these values at any time period. In conclusion, the exercise intensity of DWS was high and SWS moderate. The predominant sources of energy may be phosphocreatine stores and aerobic metabolism during these performances. It is possible that glycolysis may also play an important role in energy requirements during the 2/3 stage and immediately after a DWS performance. © 2015, Japanese Society of Physical Fitness and Sports Medicine. All rights reserved.


Tanaka C.,J. F. Oberlin University | Reilly J.J.,University of Strathclyde | Tanaka M.,Kyoto Seibo College | Tanaka S.,Japan National Institute of Health Sciences
BMC Public Health | Year: 2016

Background: The recent prevalence of obesity in Japanese children is much higher compared to 1980. The present study compared daily sedentary behavior (SB) and physical activity (PA) between the school year and summer vacation in Japanese primary school children. Methods: Participants were 98 Japanese boys (8.9 ± 1.8 years at baseline) and 111 girls (9.1 ± 1.8 years). SB and PA were measured in May (school term) and July/August (summer vacation), 2011. SB and PA were assessed using a triaxial accelerometer (Active style Pro HJA-350IT, Omron Healthcare) for 7 consecutive days. The average number of minutes spent in SB (no more than 1.5 metabolic equivalents (METs)), light intensity activity (LPA; more than 1.5 to less than 3.0 METs) and moderate-to-vigorous physical activity (MVPA; 3.0 METs or more), and step counts were calculated for each individual. Moreover, the determinants/moderators of changes in SB and PA were examined. Results: Daily SB was significantly higher in the summer vacation than in the school year for both boys and girls (p < 0.05). Ambulatory and total LPA and MVPA, non-ambulatory LPA and step counts were lower in summer vacation in both genders (p < 0.001). Moreover, non-ambulatory MVPA was significantly lower in the summer vacation than in the school year for girls (p < 0.001). The decrease in non-ambulatory MVPA in boys and increase in SB in girls were significantly lower in those who participated in sports compared to those who did not (p < 0.040 or p < 0.033). The change in SB for boys was significantly associated with having a TV in the bedroom (p < 0.022). Conclusions: These findings show that primary school children in Japan are less active in the summer vacation, as indicated by both higher SB and lower LPA and ambulatory MVPA in both genders. Moreover, the seasonal change in non-ambulatory MVPA for Japanese children was affected by gender. This study also suggests that sports participation and bedroom TV ownership may moderate seasonal changes in PA and SB. The results emphasize the need to take summer vacation into account when planning interventions aimed at decreasing SB or increasing PA in Japanese children. © 2016 The Author(s).


Asakura H.,Japan National Institute of Health Sciences | Ekawa T.,Japan National Institute of Health Sciences | Sugimoto N.,Japan National Institute of Health Sciences | Momose Y.,Japan National Institute of Health Sciences | And 4 more authors.
Biochemical and Biophysical Research Communications | Year: 2012

Salmonella enterica serovar Typhimurium is a major cause of human gastrointestinal illness worldwide. This pathogen can persist in a wide range of environments, making it of great concern to public health. Here, we report that the salmonella pathogenicity island (SPI)-1 effector protein SipB exhibits a membrane topology that confers bacterial osmotolerance. Disruption of the sipB gene or the invG gene (SPI-1 component) significantly reduced the osmotolerance of S. Typhimurium LT2. Biochemical assays showed that NaCl osmolarity increased the membrane topology of SipB, and a neutralising antibody against SipB reduced osmotolerance in the WT strain. The WT strain, but not the sipB mutant, exhibited elevated cyclopropane fatty acid C19:0 during conditions of osmotic stress, correlating with the observed levels of survival and membrane integrity. This result suggests a link between SipB and the altered fatty acid composition induced upon exposure to osmotic stress. Overall, our findings provide the first evidence that the Salmonella virulence translocon SipB affects membrane fluidity and alters bacterial osmotolerance. © 2012 Elsevier Inc.


Hayashi M.,Gifu University | Kubota-Hayashi S.,Gifu University | Natori T.,Gifu University | Mizuno T.,Gifu University | And 7 more authors.
International Journal of Food Microbiology | Year: 2013

A Food Pathogen Enrichment (FPE) broth, which supports the growth of Campylobacter without lysed blood and CO2, was developed. The FPE broth supports the growth of Campylobacter to the same degree as Bolton and Preston broths. Using the FPE broth, we developed a novel rapid protocol to detect small numbers of Campylobacter in 25g of food. The sensitivity of FPE enrichment and PCR to detect Campylobacter spp. from spiked chicken meat was determined. The detection sensitivities for non-stressed C. jejuni and C. coli from fresh meat ranged from 5.8 to 1.1×101CFU per 25g of chicken meat, and those for freeze-stressed C. jejuni and C. coli from frozen meat ranged from 9.9×101 to 2.0×102CFU. The FPE broth enrichment culture (24h) of chicken meat, followed by PCR, resulted in a significantly higher detection score (80% positive) than conventional Bolton enrichment and subsequent colony isolation using mCCDA agar plates (18% positive). Differences between our new protocol and the Bolton enrichment method were due to the overgrowth of many resistant bacteria, especially extended-spectrum beta-lactamase-producing bacteria in the Bolton enrichment broth. © 2013.


Asakura H.,Japan National Institute of Health Sciences | Kawamoto K.,Obihiro University of Agriculture and Veterinary Medicine | Murakami S.,Tokyo University of Agriculture | Tachibana M.,Japan National Institute of Health Sciences | And 4 more authors.
Research in Microbiology | Year: 2016

Campylobacter jejuni is one of the leading causes of foodborne gastrointestinal illness worldwide. Here we performed ex vivo proteomic analysis of C. jejuni 81-176 in chicken, a main reservoir for human infection. At 0, 1 and 4 weeks post-infection (p.i.) with the GFP-expressing 81-176 strain, inocula were recovered from chicken ceca by cell sorting using flow cytometry. iTRAQ-coupled 2D-LC-MS/MS analyses that detected 55 C. jejuni proteins, among which either 3 (FabG, HydB, CJJ81176_0876) or 7 (MscS, CetB, FlhF, PurH, PglJ, LpxC, Icd) proteins exhibited >1.4-fold-increased expression at 1 or 4 week(s) p.i. compared with those at 0 weeks p.i., respectively. Deletion of the fabG gene clearly decreased the proportion of bacterial unsaturated fatty acids (UFAs) and chicken colonization. The UFA proportion of the parental strain was not altered when grown at 42 °C. These findings suggest that FabG might play a pivotal role in UFA production, linked to bacterial adaptation in the poultry host. To our knowledge, this is the first example of ex vivo C. jejuni proteomics, in which fatty acid metabolism might affect bacterial adaptation to the chicken host. © 2015 Institut Pasteur.


PubMed | Tokyo University of Agriculture, Kyoto Seibo College, Obihiro University of Agriculture and Veterinary Medicine, Japan National Institute of Health Sciences and Tokai University
Type: Journal Article | Journal: Research in microbiology | Year: 2016

Campylobacter jejuni is one of the leading causes of foodborne gastrointestinal illness worldwide. Here we performed exvivo proteomic analysis of C. jejuni 81-176 in chicken, a main reservoir for human infection. At 0, 1 and 4 weeks post-infection (p.i.) with the GFP-expressing 81-176 strain, inocula were recovered from chicken ceca by cell sorting using flow cytometry. iTRAQ-coupled 2D-LC-MS/MS analyses that detected 55 C. jejuni proteins, among which either 3 (FabG, HydB, CJJ81176_0876) or 7 (MscS, CetB, FlhF, PurH, PglJ, LpxC, Icd) proteins exhibited >1.4-fold-increased expression at 1 or 4 week(s) p.i. compared with those at 0 weeks p.i., respectively. Deletion of the fabG gene clearly decreased the proportion of bacterial unsaturated fatty acids (UFAs) and chicken colonization. The UFA proportion of the parental strain was not altered when grown at 42C. These findings suggest that FabG might play a pivotal role in UFA production, linked to bacterial adaptation in the poultry host. To our knowledge, this is the first example of exvivo C. jejuni proteomics, in which fatty acid metabolism might affect bacterial adaptation to the chicken host.


PubMed | Gifu University, Kyoto Seibo College and Obihiro University of Agriculture and Veterinary Medicine
Type: | Journal: BioMed research international | Year: 2013

A quick foodborne pathogen screening method after six-hour enrichment culture with a broad-range food pathogen enrichment broth is described. Pathogenic factors of Salmonella enterica, Shigella spp., enteroinvasive Escherichia coli, and enterohemorrhagic E. coli are amplified with a cocktail primer and rapid polymerase chain reaction (PCR), which finishes amplification in 30min. The PCR amplicon was differentiated with a dipstick DNA chromatography assay in 5-10min. Starting from a four- to six-hour enrichment culture, this assay was finished within 45min. Detection sensitivity of this protocol was less than 2.5CFU/25g for S. enterica and 3.3CFU/25g for enterohemorrhagic E. coli in spiked ground meat experiments.

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