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Takeda S.,Kyoto Prefectural University | Takeda S.,Kyoto Prefectural Agriculture Forestry and Fisheries Technology Center | Noguchi M.,Kyoto Prefectural University | Hamamura Y.,Nagoya University | Higashiyama T.,Nagoya University
Planta | Year: 2014

In many flowering plants, flowers consist of two peripheral organs, sepals and petals, occurring in outer two whorls, and two inner reproductive organs, stamens and carpels. These organs are arranged in a concentric pattern in a floral meristem, and the organ identity is established by the combined action of floral homeotic genes expressed along the whorls. Floral organ primordia arise at fixed positions in the floral meristem within each whorl. The RABBIT EARS (RBE) gene is transcribed in the petal precursor cells and primordia, and regulates petal initiation and early growth in Arabidopsis thaliana. We investigated the spatial and temporal expression pattern of a RBE protein fused to the green fluorescent protein (GFP). Expression of the GFP:RBE fusion gene under the RBE cis-regulatory genomic fragment rescues the rbe petal defects, indicating that the fusion protein is functional. The GFP signal is located to the cells where RBE is transcribed, suggesting that RBE function is cell-autonomous. Ectopic expression of GFP:RBE under the APETALA1 promoter causes the homeotic conversion of floral organs, resulting in sterile flowers. In these plants, the class B homeotic genes APETALA3 and PISTILLATA are down-regulated, suggesting that the restriction of the RBE expression to the petal precursor cells is crucial for flower development. © Springer-Verlag Berlin Heidelberg 2013. Source

Yamamoto T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Yamada T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Kinoshita T.,Japan National Research Institute of Fisheries Science | Ueda Y.,Niigata Laboratory | And 3 more authors.
Crustaceana | Year: 2015

Growth and moulting of wild-born immature snow crabs (Chionoecetes opilio(Fabricius, 1788)) were investigated by laboratory culture experiments. Crabs with 16.2-42.9 mm carapace width caught from the Sea of Japan were cultured at a temperature of their natural habitat (approximately 1°C). The growth indices (size increments at moulting in mm and in % of premoult carapace width) and intermoult period were significantly affected by premoult carapace width, but sex did not affect these variables. Furthermore, we demonstrated that premoult carapace width and days after moulting significantly affected moulting probability and we developed a moulting probability model based on these variables. From this model, the number of days of intermoult periods when moults occurred in 50% of crabs of instars VI, VII and VIII was estimated at 234, 284 and 346 days, respectively. © Copyright 2015 by Koninklijke Brill NV, Leiden, The Netherlands. Source

Kaya H.,Tokyo University of Science | Iwano M.,Nara Institute of Science and Technology | Iwano M.,Osaka University | Takeda S.,Kyoto Prefectural University | And 7 more authors.
Plant Signaling and Behavior | Year: 2015

Reactive oxygen species (ROS) accumulate at the tip of growing pollen tubes. In Arabidopsis, NADPH oxidases RbohH and RbohJ are localized at the plasma membrane of pollen tube tip and produce ROS in a Ca2+-dependent manner. The ROS produced by Rbohs and Ca2+ presumably play a critical role in the positive feedback regulation that maintains the tip growth. Ultrastructural cytochemical analysis revealed ROS accumulation in the apoplast/cell wall of the pollen grains on the stigmatic papillae in the wild type, but not in the rbohH rbohJ double mutant, suggesting that apoplastic ROS derived from RbohH and RbohJ are involved in pollen tube elongation into the stigmatic papillae by affecting the cell wall metabolism. © 2015 Taylor & Francis Group, LLC. Source

Ueda Y.,Niigata Laboratory | Fujimoto H.,National Research Institute of Aquaculture | Yamada T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Kinoshita T.,Japan National Research Institute of Fisheries Science | And 3 more authors.
Journal of Crustacean Biology | Year: 2015

The effect of water temperature on the growth of juvenile snow crabs Chionoecetes opilio (Fabricius, 1788) was investigated in laboratory culture experiments. Laboratory-born juveniles were cultured from instar I to VIII at four temperatures (approximately 1, 3, 5 and 8°C). The growth indices (size increments at moulting in mm and in % of premoult carapace width) were significantly higher in crabs reared at 5°C than in those reared at other temperatures. The relationship between the mean temperature (T) and intermoult period (D) of each instar was described by the heat summation theory equation: D=K/(T-α). The thermal constant K is the summation of the effective temperature for development (above the threshold temperature, α) up to a selected biological end point. The thermal constant tended to increase and the threshold temperature tended to decrease with increasing mean premoult carapace width of each instar, reaching asymptotes of 1573 day-degrees and -4.7°C, respectively. Size-and temperature-dependent growth models were developed for snow crab juveniles. © The Crustacean Society, 2015. Published by Brill NV. Source

Tsuda K.,Kyoto Prefectural Agriculture Forestry and Fisheries Technology Center | Tsuji G.,Kyoto Prefectural University | Higashiyama M.,Kyoto Prefectural Agriculture Forestry and Fisheries Technology Center | Ogiyama H.,Kyoto Prefectural University | And 4 more authors.
Biological Control | Year: 2016

Bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum is one of the most important diseases of Chinese cabbage. To develop a microbial pesticide that is not only safe but also easily acceptable by consumers, we screened strains of lactic acid bacteria for biocontrol potential. One of 1166 isolates, Lactobacillus plantarum strain BY significantly reduced soft rot disease severity in Chinese cabbage in six different fields in Japan. This isolate also suppressed disease in onion, potato, and tomato. BY populations persisted on wounded Chinese cabbage leaves after spraying. Moreover, BY inhibits pathogen growth in an antibiosis assay. These results strongly suggest that BY inhibits the invasion of the pathogen at wounded sites on leaves and its proliferation in host tissues. This is the first report indicating the application of lactic acid bacteria for biological control of a plant disease under field conditions. © 2016 Elsevier Inc. Source

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