Kyono ART Clinic Takanawa

Tokyo, Japan

Kyono ART Clinic Takanawa

Tokyo, Japan
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Nakamura Y.,Kyono ART Clinic | Obata R.,Kyono ART Clinic Takanawa | Okuyama N.,Kyono ART Clinic Takanawa | Aono N.,Kyono ART Clinic | And 2 more authors.
Reproductive BioMedicine Online | Year: 2016

There have been 60 births after transplantation of cryopreserved ovarian tissue: 58 using the slow freezing method, and two using the vitrification method. DMSO and EG are widely used as cryoprotectants. However DMSO is a known epimutagen, and EG has been reported to be toxic in high concentrations. In this study, we measured residual DMSO and EG in ovarian tissue after vitrification and slow freezing. Cryoprotectants remained at a high concentration in the vitrified/warmed ovarian tissue just before transplantation (DMSO: 9.8 mg/g, EG: 9.8 mg/g). We must consider the impact of the cryoprotectants on the mother and the baby. © 2017 The Authors.


Kyono K.,Kyono ART Clinic | Hashimoto T.,Kyono ART Clinic | Kuchiki M.,Kyono ART Clinic | Sakamoto E.,Kyono ART Clinic | And 7 more authors.
Journal of Mammalian Ova Research | Year: 2016

Purpose: To highlight the imperative of informed consent in the fertility preservation of cancer patients before ovarian tissue autotransplantation. Methods: Three papers of tumor recurrence after autotransplantation of frozen-thawed ovarian tissue were compared with the main papers before tumor recurrence was reported in the cancer patients. Results: Histology was performed before autotransplantation in cases 1 and 3, but not in case 2. Histology alone is insufficient for the detection of minimal residual disease (MRD). Furthermore, transplanted ovarian tissue is different from that examined for MRD detection, and how much of the resected ovarian tissue was examined for MRD detection is unclear. The possibility that grafted tissue caused tumor recurrence cannot be ruled out in any of the three cases, because autotransplantation in cancer patients, at present, uses ovarian tissue different from that examined for MRD. Conclusions: We recommend informed consent for cancer patients, because: 1) the transplanted ovarian tissue is different from the ovarian tissue examined for MRD detection; 2) the amount of resected ovarian tissue analyzed for MRD is very small; and 3) MRD detection methods vary. In conclusion, freezing and storage of ovaries should be encouraged, transplantation must be performed carefully, and informed consent is essential. © 2016 Japan Society for Ova Research.

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