Kwang Dong Pharmaceutical Co.

Gwangju, South Korea

Kwang Dong Pharmaceutical Co.

Gwangju, South Korea
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Kim T.-H.,Ajou University | Lee K.M.,Kwang Dong Pharmaceutical Co. | Hong N.D.,Kwang Dong Pharmaceutical Co. | Jung Y.-S.,Ajou University
Journal of Ethnopharmacology | Year: 2016

Ethnopharmacological relevance Kyung-Ok-Ko (KOK), a traditional herbal prescription, contains six main ingredients; Rehmannia glutinosa var. purpurae, Lycium chinense, Aquillaria agallocha, Poria cocos, Panax ginseng, and honey. KOK has been widely taken as a traditional oriental medicine for improving blood circulation or age-related symptoms, such as dementia and stroke. However, the effect of KOK on platelet activity has not been clarified. Materials and methods To evaluate the effect of KOK on platelet function, we evaluated its effect on functional markers of platelet activation such as aggregation and shape change. As a mechanism study for the effect of KOK, we examined its effect on granule secretion, intracellular Ca2+ increase, and PLCγ and Akt activation. To investigate the effect of orally administered KOK (0.5, 1, 2 g/kg), we examined its ex vivo effect on platelet aggregation in rat, and its in vivo anti-thrombotic effect in mice thromboembolism model. Furthermore, the effect of KOK on bleeding time was examined to estimate its potential side effect. Results KOK (0.3, 1, 3, 10 mg/ml) inhibited collagen-induced platelet aggregation and shape change in rat platelets in a concentration-dependent manner. The mechanism for the anti-platelet effect of KOK seems to involve the inhibition of ATP release, intracellular Ca2+ elevation, and the phosphorylation of PLCγ and Akt. In rat ex vivo study, KOK (2 g/kg, p.o. for 1 day, and 0.5, 1, 2 g/kg, p.o. for 7 days) also had significant inhibitory effects on collagen-induced platelet aggregation. In addition, KOK showed a significant protective effect against thrombosis attack in mice. The prolongation of bleeding time by KOK was much less than that by ASA, suggesting a beneficial potential of KOK than ASA in view of side effect. Conclusions These findings suggest that KOK elicits remarkable anti-platelet and anti-thrombotic effects with less side effect of bleeding, and therefore, it may have a therapeutic potential for the prevention of platelet-associated cardiovascular diseases. © 2015 Published by Elsevier Ireland Ltd.

Choi R.-Y.,Sunchon National University | Woo M.-J.,Kwang Dong Pharmaceutical Co. | Ham J.R.,Sunchon National University | Lee M.-K.,Sunchon National University
Biomedicine and Pharmacotherapy | Year: 2017

The anti-steatotic and anti-inflammatory effects of fruit water extract (FW) and seed ethanol extract (SE) of Hovenia dulcis Thunb. in chronic alcohol-fed rats were investigated. Rats were fed a liquid diet containing 36% calories from alcohol and orally administered FW or SE (300 and 500 mg/kg/day). Both FW and SE reduced hepatic lipid contents and droplets, serum lipid concentration and inflammatory markers (hs-CRP, TNF-α and IL-6) levels compared with the alcohol control group. Alcohol led to significant decreases in the hepatic fatty acid oxidative gene (Ppargc1a, Cpt1a and Acsl1) levels, while it significantly increased the Myd88 and Tnfa gene levels. However, FW or SE supplementation significantly up-regulated gene expression of Ppargc1a, Ppara, Cpt1a and Acsl1, and down-regulated gene expression of Myd88, Tnfa and Crp compared with the alcohol control group. FW or SE supplementation also significantly decreased hepatic activities of fatty acid synthase and phosphatidate phosphohydrolase in chronic alcohol-fed rats. Plasma alcohol and acetaldehyde levels, hepatic enzyme activity and protein expression of CYP2E1 were lowered by FW or SE supplementation. These results indicate that both FW and SE play an important role in improvement of alcoholic hepatic steatosis and inflammation via regulation of lipid and inflammation metabolism. © 2017 Elsevier Masson SAS

Cai M.,Kyung Hee University | Shin B.Y.,Kyung Hee University | Kim D.H.,Kyung Hee University | Kim J.M.,Kyung Hee University | And 7 more authors.
Journal of Ethnopharmacology | Year: 2011

Aim of the study: Kyung-Ok-Ko (KOK), a traditional herbal prescription composed of Rehmannia glutinosa var. purpurae, Panax ginseng, Poria cocos, Lycium chinense, Aquillaria agallocha and honey, has been used to treat age-related symptoms, such as amnesia or dementia, and has been shown to ameliorate scopolamine-induced memory impairment in mice. However, the effects of KOK on transient cerebral global ischemia-induced brain damage are unclear. Materials and methods: Transient cerebral global ischemia was induced by occluding the bilateral common carotid artery for 5 min followed by reperfusion for 7 days. KOK (0.25, 0.5, 1, or 2 g/kg) was administered orally immediately after reperfusion and once a day over the next 7 days. Y-maze or novel object recognition tasks were to analyze learning and memory capabilities at 4 or 5 days after reperfusion, respectively. Histochemistry and immunohistochemistry were used for evaluation of the effect of KOK on neuronal degeneration. Results: Histochemical studies showed that KOK increased the number of viable cells detected by Nissl staining and decreased the number of degenerated neuronal cells detected by Fluoro-Jade B staining in the hippocampal CA1 region. In the immunohistochemical study, the sub-chronic KOK administration attenuated the ischemia-induced activation of microglia and astrocytes and the increase of cytokine IL-1β (P < 0.05). In addition, KOK administration significantly attenuated the ischemia-induced cognitive impairments observed in the Y-maze and novel object recognition tasks (P < 0.05). Conclusion: These findings suggest that the neuroprotective effects of KOK may be mediated by its anti-inflammatory activities, resulting in the attenuation of memory impairment. © 2011 Elsevier Ireland Ltd. All rights reserved.

Lee H.-S.,Korea University | Kim M.K.,Korea University | Kim Y.-K.,Korea University | Jung E.Y.,Korea University | And 5 more authors.
Journal of Ethnopharmacology | Year: 2011

Ethnopharmacological relevance: For thousands of years antlers have been used in Asian countries to promote rapid healing, treat weight loss, slow growth in children, strengthen weak bones, and alleviate cold hands and feet. Aim of the study: The present study was performed to examine the effect of fermentation on the ability of antler to act as a stimulator of bone growth. Materials and methods: This study used pre-osteoblast MC3T3-E1 cells to examine factors related to bone growth, such as cell proliferation, production of alkaline phosphatase (ALP), and deposition of extracellular matrix proteins (e.g.; collagens, osteonectin, bone sialoprotein (BSP)), via the treatment of non-fermented and fermented antler. Results: Antler fermentation using Cordyceps militaris was carried out at 25 °C for seven days. The total content of sugar, sialic acid, and protein increased with fermentation time. Cell proliferation was greater in the fermented antler- (FA-) treated groups than in the NFA- (non-fermented antler-) treated groups, in which proliferation increased significantly up to 137% of the basal value. Significant increases in mRNA expression and ALP activity were found at FA concentrations of 50-100 μg/ml; at 100 μg/ml the activity had increased 119% compared to the control activity. For NFA and FA the expression levels of type I collagen mRNA significantly increased in a dose-dependent manner at all treatment doses. However, significant differences between the antler groups were not observed. Mineralization significantly increased by NFA and FA treatment to 183% and 241%, respectively, when compared to colostrum, as a positive control (165%). Conclusions: Antler treatment increased the proliferation of osteoblasts and bone matrix proteins, such as type I collagen and BSP. Antler fermented with Cordyceps militaris showed enhanced activity, and its stimulatory effects on cell proliferation and ALP production were greater than those of NFA. We surmise that these increases in activity were related to increased sialic acid content. Therefore, the results of this study suggest that the physiological effects of antler, including bone growth, may be increased through the fermentation process. © 2010 Elsevier Ireland Ltd. All rights reserved.

Kwang Dong Pharmaceutical Co. and C.L. Pharm Corporation | Date: 2012-12-10

A method and apparatus for manufacturing an edible film are disclosed. The method includes a plurality of applying operations of applying solution for forming the edible film onto lower wrapping paper in a thin film shape. The solution-applying operations include a first solution-applying and a second solution-applying operation. Therefore, in the case of an edible medicine film product, particularly, when a tiny dosage of medicine must be loaded onto an edible medicine film, a loss of medicine can be reduced as much as possible. A variety of kinds of films can be manufactured through a multi-solution-applying operation, when different kinds of medicines, for example, those which are respectively absorbed in the mouth mucous membrane, the stomach and the intestine, are required.

Lee S.H.,Kwang Dong Pharmaceutical Co. | Suh H.J.,Korea University | Lee H.-S.,Korea University | Park Y.,Korea University | And 2 more authors.
Journal of Medicinal Food | Year: 2012

This study examined the effect of fermentation on the ability of antler to act as a stimulator of hematopoietic activity. Hemolytic anemia was induced by phenylhydrazine (PHZ) in female Sprague-Dawley rats. The vehicle or antler extract (nonfermented or fermented) mixed in drinking water was administered from Days 2 to 15 after PHZ injection. On Day 15, red blood cell counts in the fermented antler group (6.33×106/μL) were significantly higher than those in the nonfermented antler group (5.90×10 6/μL) (P<.05), and rats treated with fermented antler extract tended to have higher hemoglobin compared with rats treated with nonfermented antler extract, but not significantly. In addition, rats treated with fermented antler extract had slightly lower serum erythropoietin levels compared with nonfermented antler extract, which were not statistically different from serum erythropoietin levels of nonanemic rats. We conclude therefore that the hematopoietic activity of antler might be increased by the fermentation process. © Copyright 2012, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2012.

Kim S.-M.,Yeungnam University | Lim S.-M.,Yeungnam University | Yoo J.-A.,Yeungnam University | Woo M.-J.,Kwang Dong Pharmaceutical Co. | Cho K.-H.,Yeungnam University
Food and Function | Year: 2015

Background Although the health effects of vitamin C are well known, its physiological effect on serum lipoproteins and microRNA still remain to be investigated, especially daily consumption of a high dosage. Objectives To investigate the physiological effect of vitamin C on serum lipoprotein metabolism in terms of its anti-oxidant and anti-glycation activities, and gene expression via microRNA regulation. Methods We analyzed blood parameters and lipoprotein parameters in young subjects (n = 46, 22 ± 2 years old) including smokers who consumed a high dose of vitamin C (1250 mg) daily for 8 weeks. Results Antioxidant activity of serum was enhanced with the elevation of Vit C content in plasma during 8 weeks consumption. In the LDL fraction, the apo-B48 band disappeared at 8 weeks post-consumption in all subjects. In the HDL fraction, apoA-I expression was enhanced by 20% at 8 weeks, especially in male smokers. In the lipoprotein fraction, all subjects showed significantly reduced contents of advanced glycated end products and reactive oxygen species (ROS). Triglyceride (TG) contents in each LDL and HDL fraction were significantly reduced in all groups following the Vit C consumption, suggesting that the lipoprotein was changed to be more anti-inflammatory and atherogenic properties. Phagocytosis of LDL, which was purified from each individual, into macrophages was significantly reduced at 8-weeks post-consumption of vitamin C. Anti-inflammatory and anti-senescence effects of HDL from all subjects were enhanced after the 8-weeks consumption. The expression level of microRNA 155 in HDL3 was reduced by 49% and 75% in non-smokers and smokers, respectively. Conclusion The daily consumption of a high dose of vitamin C for 8 weeks resulted in enhanced anti-senescence and anti-atherosclerotic effects via an improvement of lipoprotein parameters and microRNA expression through anti-oxidation and anti-glycation, especially in smokers. © The Royal Society of Chemistry.

Won Y.-H.,Kwang Dong Pharmaceutical Co. | Park M.-S.,Duksung Womens University
Archives of Pharmacal Research | Year: 2010

A new series of 3-allylthio-6-(mono or disubstituted) aminopyridazines was synthesized by reacting 3-allylthio-6-chloropyridazine with several amines to develop new anticancer agents. These new compounds showed antiproliferative activities against lung cancer (A549), hepatoblastoma (Hep3b), prostate cancer (PC3), colon cancer (SW480) and cervical cancer (HeLa) cells in MTT assays, and could be promising candidates for chemotherapy of carcinomas. Compound 5 (3-allylthio-6-homopiperidinylaminopyridazine) showed higher potencies than 5-FU for inhibiting the growth of these cell lines. This suggests the potential anticancer activity of compound 5. © 2010 The Pharmaceutical Society of Korea and Springer Netherlands.

PubMed | Kwang dong Pharmaceutical Co. and Kyung Hee University
Type: Journal Article | Journal: Phytotherapy research : PTR | Year: 2016

Hovenia dulcis Thunb. (HDT) was known to have anti-fatigue, anti-diabetes, neuroprotective, and hepatoprotective effects. In the present study, the anti-fatty liver mechanism of HDT was elucidated in oleic acid (OA)-treated Hep G2 cells and acute hyperlipidemia mouse model using Triton WR-1339. Here, HDT activated p-AMP-activated protein kinase (p-AMPK), proliferator activated receptor-, carnitine palmitoyltransferase and also inhibited the expression of lipogenesis and cholesterol synthesis proteins, such as 3-hydroxy-3-methylglutaryl-CoA reductase, sterol regulatory element binding protein-1c, SREBP-2, and fatty acid synthase in OA-treated Hep G2 cells. Conversely, AMPK inhibitor compound C blocked the anti-fatty liver effect of HDT to induce AMPK phosphorylation and decrease 3-hydroxy-3-methylglutaryl-CoA reductase and lipid accumulation by oil red O staining in OA-treated Hep G2 cells. Additionally, HDT pretreatment protected against the increase of serum total cholesterol, triglyceride, low-density lipoprotein cholesterol and phospholipid in an acute hyperlipidemia mouse model with enhancement of glutathione reductase, glutathione peroxidase, superoxide dismutase, and catalase activities. Taken together, HDT inhibits OA-induced hepatic lipid accumulation via activation of AMPK and proliferator activated receptor-/carnitine palmitoyltransferase signaling and enhancement of antioxidant activity as a potent candidate for nonalcoholic fatty liver disease and hyperlipidemia. Copyright 2016 John Wiley & Sons, Ltd.

Kwang Dong Pharmaceutical Co. | Date: 2015-04-15

Food for babies; lacteal flour for babies; medicines for sensory organs; oral contraceptive pills; anti-cough drops; protein dietetic supplements; pharmaceutical preparations for treating diabetes; pharmaceutical agents affecting metabolism; veterinary preparations for the treatment of neurodegenerative and inflammatory diseases of domestic animals and livestock; dietetic supplements for animals; pharmaceutical preparations acting on the peripheral nervous system; mineral food-supplements; pharmaceuticals for the treatment of erectile dysfunction; pharmaceutical preparations for urogenital organs; vitamin preparations; crude medicines for the relief of pain; anti-inflammatory and antipyretic preparations; pharmaceutical preparations acting on the digestive organs; cardiovascular agents for medical purposes; ophthalmic preparations; antiallergic agents for medical purposes; nutritive supplement agents for epidermis; disinfectants for sanitary purposes; mouthwashes for medical purposes; dietetic foods, namely, food supplements and nutritional supplements adapted for medical purposes; dietetic beverages, namely, beverage supplements and nutritional supplements adapted for medical purposes; medicinal herb extracts; chemical preparations for the diagnosis of pregnancy; medical nutrients, tonics and alternative preparations for the treatment of osteoporosis; cord blood for medical purposes; medical agents for tumor treatment; medical agents affecting central nervous system; medical preparations for slimming purposes; medicines for dental purposes; contact lens cleaning preparations; pharmaceutical preparations for use in dermatology; chemical contraceptives; antibiotic preparations; antipyretic analgesics; pharmaceutical preparations for treating respiratory diseases; deodorizer for fiber; microorganisms for medical purposes; first aid kits; anti-rheumatism bracelets; court plaster; sanitary pads; sanitary panties; diapers for medical purposes; diapers for babies; adhesive bands for medical purposes; herb teas for medical purposes; herb teas for medicinal purposes; diagnostic preparations for medical purposes. Germinated cereals; flour for food; processed cereals; high-protein cereal bars; cereal preparations, namely, cereal bars and high-protein cereal bars; cereal based snack food; farinaceous foods, namely, farinaceous food pastes for human consumption; ra-myun; cereal bars; hamburger sandwiches; yeast for food; confectionery, namely, candies, chocolate snack foods and chocolate; frozen yogurt confectionery ices; bread; ice cream; chocolates; instant pudding mixes; chewing gum; candies; cakes; cookies; crackers; popcorn; puddings; Korean traditional sweets and cookies; royal jelly for food purposes; natural sweetener; propolis for food purposes; rice cakes; soy sauce; compound chemical seasoning; sauces; aromatic preparations for food, namely, food flavorings and spices; salt for preserving foodstuff; tea; processed tea leaves as tea substitutes; tea substitutes; green tea; iced tea; black tea; prepared coffee; milk chocolates; coffee beverages with milk; cocoa beverages with milk; chocolate-based beverages; coffee; coffee-based beverages; cocoa; cocoa-based beverages; tea-based beverages; edible ice. Beers; water for beverages; mineral water for beverages; spring water for beverages; mineral water; vegetable juice beverages; fruit drinks and fruit juices; fruit juices; non-alcoholic beverages containing fruit juices; frozen fruit beverages; lemonades; syrups for lemonade; pastilles for effervescing fruit-based beverages; powders for effervescing fruit-based beverages; non-alcoholic fruit extracts used in the preparation of beverages; non-alcoholic fruit juice beverages; non-alcoholic beverages, namely, carbonated beverages, soft drinks, energy drinks and syrups; non-alcoholic cocktail mixes; seltzer water; soda water; soda pops; smoothies; isotonic beverages; sujeonggwa; non-alcoholic fruit extracts for beverages; fruit syrups for beverages; fruit powders for beverages; sorbets for beverages; ginseng juices; prepared beverages of vegetable and fruit; soft drinks; aerated water.

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