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Raghunath P.,Dr Vrk Womens Medical College Teaching Hospital And Research Center | Seshu Kumari K.,Dr Vrk Womens Medical College Teaching Hospital And Research Center | Subbannayya K.,KVG Medical College and Hospital
World Journal of Microbiology and Biotechnology

Three serum free media viz, sucrose solution, starch solution and SST broth have been formulated. The objective of the present study was to evaluate these three different serum free media for induction of germ tubes by Candida albicans and to compare their efficacy with the pooled human serum. Out of 50 C. albicans isolates 47 (94 %) and 49 (98 %) produced germ tubes in pooled human serum and SST broth, respectively. Germ tube production was positive in 40 (80 %) and 36 (72 %) isolates, respectively in sucrose solution and starch solution. This study reports SST broth as a new stable and less expensive germ tube induction medium, which requires less time for preparation and can be used without any safety concerns. SST broth is found to be more effective than pooled human serum for induction of germ tubes by C. albicans isolates. © 2014 Springer Science+Business Media Dordrecht. Source

Ravikiran S.R.,KVG Medical College and Hospital | Kumar P.M.J.,Kvg Medical College | Meundi A.D.,Kvg Medical College
Indian Journal of Pediatrics

Objective: To determine if acute pain response after administration of the BCG vaccine and the Hepatitis-B vaccine is affected by the order in which they are given. Methods: This Single-center randomized clinical trial was conducted in the Vaccination room in the Pediatrics Outpatient Department at KVG Medical College; Karnataka, India. 76 healthy term neonates undergoing routine immunization were included in this study. Newborns received either BCG vaccine or the Hepatitis-B vaccine first, followed by the other vaccine. The primary outcome was neonatal pain during vaccine injection as assessed by a validated measure, the Neonatal Infant Pain Scale (NIPS), scored by resident doctors blinded to the study purpose and hypothesis. In addition, a nurse rated pain using a 10-cm visual analog scale(VAS). Results: A total of 76 infants participated: 38 received the BCG vaccine first and 38 received the Hepatitis-B vaccine first. Demographic characteristics did not differ between the groups. Overall mean (SD) pain scores per neonate were significantly lower when BCG was administered first compared to the order when Hepatitis-B vaccine was administered first (for NIPS 5.55 [0.54] vs. 5.84 [0.29], P=.005; for VAS, 6.25 [0.80] vs. 6.58 [0.54], P=.04). When given first, the BCG vaccine caused significantly less pain (P<.001)than the Hepatitis-B, as assessed by the NIPS and VAS (P<.001). Conclusions: Pain was reduced when the BCG vaccine was administered before the Hepatitis-B in neonates undergoing routine vaccination. The authors recommend that the order of vaccine injections be the BCG vaccine followed by Hepatitis-B. © 2010 Dr. K C Chaudhuri Foundation. Source

Background: Successful outcome of endoscopic sinus surgery depends on complete visualization of the operative field and intraoperative control of bleeding. Major disadvantage of general anesthesia is the increased bleeding encountered, which can interfere with optimum visualization of the intranasal anatomy unless hypotensive methods are used. Objectives: To study the different anesthetic techniques in relation to their impact on blood loss and duration of surgery. The role of propofol was also evaluated. Design: Department of Anesthesia and ENT, Head and Neck Surgery, KVG Medical College and Hospital, Sullia, Karnataka, India. Materials and methods: 213 cases of endoscopic sinus surgeries done for nasal polyposis, sinusitis, dacryocystitis and septorhinoplasties under local and general anesthesia were evaluated between June 2009 and August 2010. Result: A good operative field was seen with propofol anesthesia compared to halothane anesthesia. The problems of fogging and frequent suctioning were lesser with propofol hypotensive anesthesia. Conclusion: Hypotensive anesthesia using propofol infusion is the anesthesia of choice for extensive nasal polyposis. Propofol when used both for induction as well as maintenance of general anesthesia in endoscopic sinus surgeries significantly reduces the blood loss, thereby improving the visibility of the endonasal structures and minimizes the chance of complications related to endoscopic sinus surgery. Source

Naik S.S.,KVG Medical College and Hospital
Clinical Rhinology

Background/Objectives: Plaster of Paris (POP), thermoplastic splints and self-adhesive padded aluminium splints are the most common splinting methods used after reduction of fractured nasal bones and rhinoplasty. All these methods have their proponents but may have one or more disadvantages in the way of being cumbersome, time-consuming, bulky, conspicuous and expensive. Design: A retrospective study at KVG Medical College and Hospital, department of ENT and head and neck surgery. Intervention: 94 cases of splinting done for nasal bone fractures and rhinoplasties were included in our study. POP and adhesive aluminium splints were used to stabilize the nasal framework. Results: Eleven cases of nondislocated nasal fractures and 10 cases of internal augmentation rhinoplasties were stabilized by aluminium nasal splints. 69 cases of displaced nasal fractures and 4 cases of external rhinoplasties were stabilized by POP splints. Conclusions: POP splints give the best stabilization for nasal bone fractures as well as for rhinoplasties but are bulky and conspicuous. Aluminium nasal splints are not bulky and conspicuous but cannot be used for fractures with lacerations and external rhinoplasties. Source

Ravikiran S.R.,KVG Medical College and Hospital | Jagadeesh Kumar P.M.,KVG Medical College and Hospital | Latha K.S.,Manipal University India
Indian Pediatrics

We assessed 513 children (2-6 y = 252; 7-12 y = 261) who visited the pediatric outpatient of a rural medical college hospital, for sleep problems using 'BEARS' tool. Sleep problems detected in the BEARS domains for preschool (2-6 years, n=252) and school children (7-12 years, n=261) were as follows: bedtime problems (33.3% vs 14.9%, P<0.001), excessive daytime sleepiness (32.5% vs 1.9%, P<0.001), awakening during night (25% vs 11.87%, P<0.001), regularity and duration of sleep (19.84% vs 4.98%, P<0.001), and sleep disordered breathing (4.8% vs 5%, P=0.1). We conclude that sleep problems are common among rural Indian children and should be routinely screened for during health visits. © 2011 Indian Academy of Pediatrics. Source

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