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Kurashiki, Japan

Kurashiki Sakuyo University is a private university in Kurashiki, Okayama, Japan. The predecessor of the school was founded in 1930, and it was chartered as a junior college in 1951. In 1966 it became a four-year college. Wikipedia.

Kanzaki K.,Kurashiki Sakuyo University | Kuratani M.,Hiroshima University | Matsunaga S.,University of Miyazaki | Yanaka N.,Hiroshima University | Wada M.,Hiroshima University
Journal of Muscle Research and Cell Motility | Year: 2014

The present study investigated changes in autolysis of three calpain isoforms in skeletal muscles undergoing eccentric contractions (ECC), leading to prolonged force deficits. Rat extensor digitorum longus and tibialis anterior muscles were exposed to 200-repeated ECC in situ, excised immediately after or 3 or 6 days after cessation of ECC, and used for measures of force output and for biochemical analyses. Full restoration of tetanic force in ECC-treated muscles was not attained until 6 days of recovery. Maximal calpain activity determined by a fluorogenic substrate was unaltered immediately after ECC, but increased to 313 and 450 % after 3 and 6 days, respectively. Increases in the amount of autolyzed calpain-3 were apparent immediately and developed progressively with recovery time, whereas elevations of autolyzed μ- and m-calpain occurred after 3 and 6 days, respectively. The protein content was augmented only in m-calpain. It is suggested that the three calpain isoforms may be involved in the dismantling, repair, remodeling and/or regeneration processes in ECC-treated muscles. © 2014 Springer International Publishing Switzerland. Source

Kimura Y.,Okayama University | Nagai H.,Okayama University | Miyamoto M.,Okayama University | Kimura M.,Kurashiki Sakuyo University | Yonekura M.,Ibaraki University
Bioscience, Biotechnology and Biochemistry | Year: 2010

In this study, we identified a royal jelly glycoprotein (RJG) that carries a unique complex-type N-glycans harboring the T-antigen (Galβ1-3GalNAc) unit. The amino acid sequence of the tryptic glycopeptide harboring the T-antigen unit was G-E-S-L-X-K (X might be glycosylated Asn), confirmed in the major royal jelly glycoprotein 1 (MRJP1), which is also expressed in the mushroom body of the honeybee brain. Source

Takayama F.,Okayama University | Nakamoto K.,Okayama University | Totani N.,Kobe Gakuin University | Yamanushi T.,Kagawa Prefectural College of Health Sciences | And 4 more authors.
Journal of Oleo Science | Year: 2010

Docosahexaenoic acid (DHA) regulates the lipid metabolism and inflammation that is closely associated with oxidative stress. The present study investigated the effects of DHA on the development of nonalcoholic steatohepatitis (NASH). To induce fatty liver, rats were fed choline-deficient high-fat diets (CDHF). The rats were then divided into 4 groups treated over the subsequent 6 weeks as follows: control, CDHF, CDHF+oxidative stress (NASH), and NASH+DHA (1.0 g/kg, p.o.). Rats of the control group were fed MF chow diet only. NASH rats showed severe steatohepatitis and liver fibrosis. Treatment with DHA significantly decreased the n-6/n-3 ratio in the livers and increased plasma SOD like activity compared with NASH rats. In addition, DHA attenuated the liver fibrosis during NASH development. Therefore, a higher DHA ratio in the liver of NASH rats might regulate the inflammatory response through a low n-6 ratio and diminished oxidative stress, effectively inhibiting liver fibrosis during NASH progression. These results suggested that DHA is a novel functional food for the prevention of NASH. © 2010 by Japan Oil Chemists' Society. Source

Shirai N.,Japan National Agriculture and Food Research Organization | Higuchi T.,Kurashiki Sakuyo University | Suzuki H.,Kagawa Nutrition University
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2015

The effects of simultaneous intake of green tea extracts and fish oil for 6 months on cognitive function, assessed using the revised Hasegawa's dementia scale, and plasma lipid levels in the elderly was investigated. Simultaneous intake of green tea extracts and fish oil significantly improved cognitive function compared with the placebo group at 6 months. Plasma docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) levels of both groups were increased at 3 and 6 months compared with initial levels. Further, the plasma DHA and EPA levels of the green tea extracts and fish oil group were significantly higher than the placebo group at 3 months. The plasma triacylglycerol (TG) concentration was significantly lower in the green tea extracts and fish oil group than in the placebo group at 6 months. These results indicate that the simultaneous intake of green tea extracts and fish oil may improve cognitive function and lower plasma TG concentrations in the elderly. Copyright © 2015, Japanese Society for Food Science and Technology. Source

Ishisaka A.,Kurashiki Sakuyo University | Kawabata K.,Fukui Prefectural University | Miki S.,Tokushima University | Shiba Y.,Tokushima University | And 5 more authors.
PLoS ONE | Year: 2013

Dietary flavonoids, such as quercetin, have long been recognized to protect blood vessels from atherogenic inflammation by yet unknown mechanisms. We have previously discovered the specific localization of quercetin-3-O-glucuronide (Q3GA), a phase II metabolite of quercetin, in macrophage cells in the human atherosclerotic lesions, but the biological significance is poorly understood. We have now demonstrated the molecular basis of the interaction between quercetin glucuronides and macrophages, leading to deconjugation of the glucuronides into the active aglycone. In vitro experiments showed that Q3GA was bound to the cell surface proteins of macrophages through anion binding and was readily deconjugated into the aglycone. It is of interest that the macrophage-mediated deconjugation of Q3GA was significantly enhanced upon inflammatory activation by lipopolysaccharide (LPS). Zymography and immunoblotting analysis revealed that β-glucuronidase is the major enzyme responsible for the deglucuronidation, whereas the secretion rate was not affected after LPS treatment. We found that extracellular acidification, which is required for the activity of β-glucuronidase, was significantly induced upon LPS treatment and was due to the increased lactate secretion associated with mitochondrial dysfunction. In addition, the β-glucuronidase secretion, which is triggered by intracellular calcium ions, was also induced by mitochondria dysfunction characterized using antimycin-A (a mitochondrial inhibitor) and siRNA-knockdown of Atg7 (an essential gene for autophagy). The deconjugated aglycone, quercetin, acts as an anti-inflammatory agent in the stimulated macrophages by inhibiting the c-Jun N-terminal kinase activation, whereas Q3GA acts only in the presence of extracellular β-glucuronidase activity. Finally, we demonstrated the deconjugation of quercetin glucuronides including the sulfoglucuronides in vivo in the spleen of mice challenged with LPS. These results showed that mitochondrial dysfunction plays a crucial role in the deconjugation of quercetin glucuronides in macrophages. Collectively, this study contributes to clarifying the mechanism responsible for the anti-inflammatory activity of dietary flavonoids within the inflammation sites. © 2013 Ishisaka et al. Source

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