Kurashiki, Japan

Kurashiki Sakuyo University

Kurashiki, Japan

Kurashiki Sakuyo University is a private university in Kurashiki, Okayama, Japan. The predecessor of the school was founded in 1930, and it was chartered as a junior college in 1951. In 1966 it became a four-year college. Wikipedia.

Time filter
Source Type

Nakamoto K.,Kobe Gakuin University | Nishinaka T.,Kobe Gakuin University | Sato N.,Kobe Gakuin University | Aizawa F.,Kobe Gakuin University | And 5 more authors.
British Journal of Pharmacology | Year: 2015

Background and Purpose The ω-3 polyunsaturated fatty acids exert antinociceptive effects in inflammatory and neuropathic pain; however, the underlying mechanisms remain unclear. Docosahexaenoic acid-induced antinociception may be mediated by the orphan GPR40, now identified as the free fatty acid receptor 1 (FFA1 receptor). Here, we examined the involvement of supraspinal FFA1 receptor signalling in the regulation of inhibitory pain control systems consisting of serotonergic and noradrenergic neurons. Experimental Approach Formalin-induced pain behaviours were measured in mice. Antinociception induced by FFA1 receptor agonists was examined by intrathecal injections of a catecholaminergic toxin, 5-HT lowering drug or these antagonists. The expression of FFA1 receptor protein and c-Fos was estimated by immunohistochemistry, and the levels of noradrenaline and 5-HT in the spinal cord were measured by LC-MS/MS. Key Results FFA1 receptors colocalized with NeuN (a neuron marker) in the medulla oblongata and with tryptophan hydroxylase (TPH; a serotonergic neuron marker) and dopamine β-hydroxylase (DBH; a noradrenergic neuron marker). A single i.c.v. injection of GW9508, a FFA1 receptor agonist, increased the number of c-Fos-positive cells and the number of neurons double-labelled for c-Fos and TPH and/or DBH. It decreased formalin-induced pain behaviour. This effect was inhibited by pretreatment with 6-hydroxydopamine, DL-p-chlorophenylalanine, yohimbine or WAY100635. Furthermore, GW9508 facilitated the release of noradrenaline and 5-HT in the spinal cord. In addition, GW1100, a FFA1 receptor antagonist, significantly increased formalin-induced pain-related behaviour. Conclusion and Implications Activation of the FFA1 receptor signalling pathway may play an important role in the regulation of the descending pain control system. © 2014 The British Pharmacological Society.

Takayama F.,Okayama University | Nakamoto K.,Okayama University | Totani N.,Kobe Gakuin University | Yamanushi T.,Kagawa Prefectural College of Health Sciences | And 4 more authors.
Journal of Oleo Science | Year: 2010

Docosahexaenoic acid (DHA) regulates the lipid metabolism and inflammation that is closely associated with oxidative stress. The present study investigated the effects of DHA on the development of nonalcoholic steatohepatitis (NASH). To induce fatty liver, rats were fed choline-deficient high-fat diets (CDHF). The rats were then divided into 4 groups treated over the subsequent 6 weeks as follows: control, CDHF, CDHF+oxidative stress (NASH), and NASH+DHA (1.0 g/kg, p.o.). Rats of the control group were fed MF chow diet only. NASH rats showed severe steatohepatitis and liver fibrosis. Treatment with DHA significantly decreased the n-6/n-3 ratio in the livers and increased plasma SOD like activity compared with NASH rats. In addition, DHA attenuated the liver fibrosis during NASH development. Therefore, a higher DHA ratio in the liver of NASH rats might regulate the inflammatory response through a low n-6 ratio and diminished oxidative stress, effectively inhibiting liver fibrosis during NASH progression. These results suggested that DHA is a novel functional food for the prevention of NASH. © 2010 by Japan Oil Chemists' Society.

Kirino A.,Kurashiki Sakuyo University | Takasuka Y.,Okayama Prefectural University | Nishi A.,Okayama Prefectural University | Yamashita H.,Okayama Prefectural University | And 3 more authors.
Journal of Nutritional Science and Vitaminology | Year: 2012

Polygonum cuspidatum has been broadly utilized as a herbal medicine in Asia, but the outline of the polyphenol compounds in the plant has not been characterized well. In the present study, the major polyphenolic components were isolated from the roots of P. cuspidatum, and identified as resveratrol and its glucosides, resveratroloside and polydatin. On the other hand, chlorogenic acid was found to be one of the polyphenolic components in the leaves and stems of the plant. Next, we elucidated that resveratrol derivatives and chlorogenic acid exhibit α-glucosidase inhibitory activities, and resveratroloside exhibits the same inhibitory activity as quercetin does. Furthermore, DPPH radical scavenging activities of antioxidants including resveratrol derivatives and chlorogenic acid derivatives were examined by initial rate analyses of their reactions. Subsequently, it was revealed that resveratrol derivatives have slow-acting effects on the radical scavenging activity and that chlorogenic acid derivatives exhibit very fast-acting effects.

Shirai N.,Japan National Agriculture and Food Research Organization | Higuchi T.,Kurashiki Sakuyo University | Suzuki H.,Kagawa Nutrition University
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2015

The effects of simultaneous intake of green tea extracts and fish oil for 6 months on cognitive function, assessed using the revised Hasegawa's dementia scale, and plasma lipid levels in the elderly was investigated. Simultaneous intake of green tea extracts and fish oil significantly improved cognitive function compared with the placebo group at 6 months. Plasma docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) levels of both groups were increased at 3 and 6 months compared with initial levels. Further, the plasma DHA and EPA levels of the green tea extracts and fish oil group were significantly higher than the placebo group at 3 months. The plasma triacylglycerol (TG) concentration was significantly lower in the green tea extracts and fish oil group than in the placebo group at 6 months. These results indicate that the simultaneous intake of green tea extracts and fish oil may improve cognitive function and lower plasma TG concentrations in the elderly. Copyright © 2015, Japanese Society for Food Science and Technology.

Kanzaki K.,Kurashiki Sakuyo University | Kuratani M.,Hiroshima University | Matsunaga S.,University of Miyazaki | Yanaka N.,Hiroshima University | Wada M.,Hiroshima University
Journal of Muscle Research and Cell Motility | Year: 2014

The present study investigated changes in autolysis of three calpain isoforms in skeletal muscles undergoing eccentric contractions (ECC), leading to prolonged force deficits. Rat extensor digitorum longus and tibialis anterior muscles were exposed to 200-repeated ECC in situ, excised immediately after or 3 or 6 days after cessation of ECC, and used for measures of force output and for biochemical analyses. Full restoration of tetanic force in ECC-treated muscles was not attained until 6 days of recovery. Maximal calpain activity determined by a fluorogenic substrate was unaltered immediately after ECC, but increased to 313 and 450 % after 3 and 6 days, respectively. Increases in the amount of autolyzed calpain-3 were apparent immediately and developed progressively with recovery time, whereas elevations of autolyzed μ- and m-calpain occurred after 3 and 6 days, respectively. The protein content was augmented only in m-calpain. It is suggested that the three calpain isoforms may be involved in the dismantling, repair, remodeling and/or regeneration processes in ECC-treated muscles. © 2014 Springer International Publishing Switzerland.

Maeda M.,Okayama University | Maeda M.,Kawasaki Medical School | Kimura M.,Kurashiki Sakuyo University | Kimura Y.,Okayama University
Journal of Biochemistry | Year: 2010

As a part of the study to reveal the biological significance of de-N-glycosylation in plants, we analysed the structural features of free N-glycans (FNGs) accumulated inside cells and secreted to the extracellular space using a rice cell culture system. The structural analysis of FNGs obtained from the intracellular fraction revealed that the high-mannose type N-glycans with one GlcNAc residue (GN1-type) occurred at a concentration of ∼10 nmol/g, while the truncated complex type N-glycans with a N, N′-diacetylchitobiosyl unit (GN2-type) occurred at a concentration of ∼1 nmol/g. This result suggested that two kinds of glycoenzymes, cytosolic endo-β-N-acetylglucosaminidase (ENGase) and intracellular acidic peptide:N-glycanse (PNGase), are involved in the production of FNGs in rice cell as well as in other plant cells. On the other hand, in the culture medium, Lewis a epitope-containing complex and high-mannose type FNGs with the N, N′-diacetylchitobiosyl unit were found, suggesting extracellular acidic PNGase to be involved in the release of N-glycans from folded/processed glycoproteins in extracellular space. Furthermore, in the culture medium, we found unusual GN1-FNGs that have a biantennary complex type structure harbouring the Lewis a epitope, suggesting cytosolic ENGase and golgi N-glycan-processing enzymes to be involved in the production of these plant complex type FNGs. © The Authors 2010. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Saito M.,Kagawa Nutrition University | Higuchi T.,Kurashiki Sakuyo University | Uchida N.,Nihon University
Fisheries Science | Year: 2014

Many bony fish type I collagens have a characteristic third chain designated as α3(I). However, much less is known about the primary structure and distinction of the proα(I) chains. Their cDNAs were cloned by RT-PCR from the muscle tissue of Japanese eel, Anguilla japonica. Three cDNAs coding for the triple-helical domain of fibrillar collagen were identified as proα1(I), proα2(I) and proα3(I) chains by sequencing selected tryptic peptides isolated from eel type I collagen subunit chains, α1(I), α2(I) and α3(I). Eel proα3(I) had high amino acid sequence identity (81 %) to its proα1(I). The distribution of seven Cys residues in the C-propeptide of proα3(I) was identical to that of proα1(I). There was a third Cys residue at the 1,268th position from the N-terminus in proα1(I), though a supposed Cys residue at the 1,264th position in proα3(I) was replaced by a Ser residue. Similar replacement has been observed in the proα3(I) chains of trout and zebrafish. These combined results suggest that replacement of the Cys residue allows for the identification of fish collagen proα(I) previously not identified as proα3(I). © 2014, Japanese Society of Fisheries Science.

Ishisaka A.,Kurashiki Sakuyo University | Kawabata K.,Fukui Prefectural University | Miki S.,Tokushima University | Shiba Y.,Tokushima University | And 5 more authors.
PLoS ONE | Year: 2013

Dietary flavonoids, such as quercetin, have long been recognized to protect blood vessels from atherogenic inflammation by yet unknown mechanisms. We have previously discovered the specific localization of quercetin-3-O-glucuronide (Q3GA), a phase II metabolite of quercetin, in macrophage cells in the human atherosclerotic lesions, but the biological significance is poorly understood. We have now demonstrated the molecular basis of the interaction between quercetin glucuronides and macrophages, leading to deconjugation of the glucuronides into the active aglycone. In vitro experiments showed that Q3GA was bound to the cell surface proteins of macrophages through anion binding and was readily deconjugated into the aglycone. It is of interest that the macrophage-mediated deconjugation of Q3GA was significantly enhanced upon inflammatory activation by lipopolysaccharide (LPS). Zymography and immunoblotting analysis revealed that β-glucuronidase is the major enzyme responsible for the deglucuronidation, whereas the secretion rate was not affected after LPS treatment. We found that extracellular acidification, which is required for the activity of β-glucuronidase, was significantly induced upon LPS treatment and was due to the increased lactate secretion associated with mitochondrial dysfunction. In addition, the β-glucuronidase secretion, which is triggered by intracellular calcium ions, was also induced by mitochondria dysfunction characterized using antimycin-A (a mitochondrial inhibitor) and siRNA-knockdown of Atg7 (an essential gene for autophagy). The deconjugated aglycone, quercetin, acts as an anti-inflammatory agent in the stimulated macrophages by inhibiting the c-Jun N-terminal kinase activation, whereas Q3GA acts only in the presence of extracellular β-glucuronidase activity. Finally, we demonstrated the deconjugation of quercetin glucuronides including the sulfoglucuronides in vivo in the spleen of mice challenged with LPS. These results showed that mitochondrial dysfunction plays a crucial role in the deconjugation of quercetin glucuronides in macrophages. Collectively, this study contributes to clarifying the mechanism responsible for the anti-inflammatory activity of dietary flavonoids within the inflammation sites. © 2013 Ishisaka et al.

Kimura Y.,Okayama University | Nagai H.,Okayama University | Miyamoto M.,Okayama University | Kimura M.,Kurashiki Sakuyo University | Yonekura M.,Ibaraki University
Bioscience, Biotechnology and Biochemistry | Year: 2010

In this study, we identified a royal jelly glycoprotein (RJG) that carries a unique complex-type N-glycans harboring the T-antigen (Galβ1-3GalNAc) unit. The amino acid sequence of the tryptic glycopeptide harboring the T-antigen unit was G-E-S-L-X-K (X might be glycosylated Asn), confirmed in the major royal jelly glycoprotein 1 (MRJP1), which is also expressed in the mushroom body of the honeybee brain.

Kimura M.,Kurashiki Sakuyo University | Hara T.,Okayama University | Kimura Y.,Okayama University
Bioscience, Biotechnology and Biochemistry | Year: 2011

In this study, we analyzed structural features of the N-glycans linked to glycoproteins expressed in various edible beans to identify excellent sources of useful N-glycans and N-glycopeptides. Structural analysis of N-glycans of the glycopeptides prepared from the pepsin digests of bean powder that the useful high-mannose type N-glycans occur predominantly in Phaseolus and Vigna beans; tora bean for Man9GlcNAc2-Asn, dainagon bean for Man 8GlcNAc2-Asn, and azuki bean for Man 7GlcNAc2-Asn.

Loading Kurashiki Sakuyo University collaborators
Loading Kurashiki Sakuyo University collaborators