Kurabo Industries Ltd.

Ōsaka, Japan

Kurabo Industries Ltd.

Ōsaka, Japan
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Goto T.,Japan National Agriculture and Food Research Organization | Ikehata A.,Japan National Agriculture and Food Research Organization | Morisawa Y.,Kwansei Gakuin University | Higashi N.,KURABO Industries Ltd. | Ozaki Y.,Kwansei Gakuin University
Physical Chemistry Chemical Physics | Year: 2012

The first electronic transition (à ← X) of liquid water was studied from the perspective of the hydration of cations by analyzing the attenuated total reflection far-ultraviolet (ATR-FUV) spectra of the Group I, II, and XIII metal nitrate electrolyte solutions. The à ← X transition energies of 1 M electrolyte solutions are higher (Li +: 8.024 eV and Cs +: 8.013 eV) than that of pure water (8.010 eV) and linearly correlate with the Gibbs energies of hydration of the cations. The increases in the à ← X transition energies are mostly attributable to the hydrogen bond formation energies of water molecules in the ground state induced by the presence of the cations. The deviation from the linear relation was observed for the high charge density cations, H +, Li +, and Be 2+, which reflects that the electronic energies in the excited states are also perturbed. Quantum chemical calculations show that the à ← X transition energies of the water-cation complexes depend on the hydration structures of the cations. The calculated à ← X transition energies of the water molecules hydrating high charge density cations spread more widely than those of the low charge density cations. The calculated transition energy spreads of the water-cation complexes directly correlate with the widths of the à ← X transition bands measured by ATR-FUV spectroscopy. © 2012 the Owner Societies.


Morisawa Y.,Kwansei Gakuin University | Ikehata A.,Japan National Agriculture and Food Research Organization | Higashi N.,KURABO Industries Ltd. | Ozaki Y.,Kwansei Gakuin University
Journal of Physical Chemistry A | Year: 2011

Far-ultraviolet (FUV) spectra in the 8.55-6.20 eV (145-200 nm) region were measured for several kinds of ketones in the liquid phase to investigate low-n Rydberg transitions using a uniquely developed technique of attenuated total reflection (ATR) FUV spectrometry. Assignments of the transitions are attempted for absorptions in this region by comparing the spectra for the liquid phase with those for the gas phase and ab initio calculations at the equation-of-motion coupled cluster theory with single and double substitutions at the aug-cc-pVDZ level. The transition from a nonbonding electron (n) to the 3s Rydberg orbital was found at around 6.7 eV for all investigated liquid ketones. Another intense band also appeared in the higher-energy region (ca. 8.5 eV) for all the ketones. A significant shoulder was found at around 7.4 eV for branched ketones. This shoulder band near 7.4 eV was assigned to the n-3p Rydberg transition. Band broadening and higher energy shifts were observed in the spectra of the liquid phase ketones in comparison with those of the gas phase ketones. © 2011 American Chemical Society.


Goto T.,Japan National Agriculture and Food Research Organization | Morisawa Y.,Kwansei Gakuin University | Morisawa Y.,Kinki University | Higashi N.,Kurabo Industries Ltd. | And 2 more authors.
Analytical Chemistry | Year: 2013

Chemical dynamics of an ozone (O3) pulse-photolytic reaction in aqueous solutions were studied with pump-probe transient far-ultraviolet (FUV) absorption spectroscopy. With a nanosecond pulse laser of 266 nm as pump light, transient spectra of O3 aqueous solutions (78-480 μM, pH 2.5-11.3) were acquired in the time range from -50 to 50 μs in the wavelength region from 190 to 225 nm. The measured transient spectra were linearly decomposed into the molar absorption coefficients and the concentration-time profiles of constituted chemical components with a multivariate curve resolution method. From the dependences of the time-averaged concentrations for 20 μs of the constituted chemicals on the initial concentration of O3, it was found that the transient spectra involve the decomposition of O3 and the formation of hydrogen peroxide (H2O2) and a third component that is assigned to hydroxyl radical (OH) or perhydroxyl radical (HO2). Furthermore, the pH dependence of the time-averaged concentration of the third components indicates that HO2 is more probable than OH as the third component. The time-averaged concentration ratio of each chemical component to the initial O3 concentration depends on the pH conditions from -0.95 to -0.60 for O3, 0.98 to 1.2 for H 2O2, 0.002 to 0.29 for OH, and 0.012 to 0.069 for HO 2. © 2013 American Chemical Society.


Ozaki Y.,Kwansei Gakuin University | Morisawa Y.,Kwansei Gakuin University | Ikehata A.,Japan National Agriculture and Food Research Organization | Higashi N.,Kurabo Industries Ltd.
Applied Spectroscopy | Year: 2012

Ultraviolet (UV) spectroscopy has long been used together with visible (Vis) spectroscopy to investigate electronic transitions of a molecule. Most studies of the electronic structure of molecules using UV spectroscopy have been carried out in the 190-380 nm region because commercial UV-Vis spectrometers are available only for that region. The wavelength region shorter than 190 nm is also very rich in information about the electronic states and structure of a molecule, but the absorptivity is very high in this region, and thus, this region has been employed to investigate mainly the electronic states and structure of gas molecules. Because condensed-phase materials with high molecular density do not transmit much light in the shorter wavelength region of the UV, reflection spectroscopy has been used to observe spectra of solid samples in the wavelength region shorter than 190 nm. However, for liquid samples one cannot generally use either absorption spectroscopy or specular reflection spectroscopy. Accordingly, UV spectroscopy in this region for liquid samples has been a relatively undeveloped research area. To solve the above difficulties of UV spectroscopy in the wavelength region shorter than 190 nm we have recently developed a totally new UV spectrometer based on attenuated total reflection (ATR) that enables us to measure spectra of liquid and solid samples in the 140-280 nm region.We will show that spectroscopy in the wavelength region shorter than 190 nm holds considerable promise not only in basic science but also in applications such as qualitative and quantitative analysis, on-line monitoring, environmental geochemical analysis, and surface analysis. The purpose of the present review paper is to report recent progress in UV spectroscopy of solid and liquid phases in the 140-280 nm region. In this review, we refer to the 120-200 nm region to as the far-UV (FUV) region. The term "vacuum UV region" is no longer appropriate for the 120-200 nm region because most recent spectrometers used in this region are not evacuated but instead incorporate a nitrogen purge. This review consists of eight parts: (1) introduction to FUV spectroscopy, (2) brief history of FUV spectroscopy, (3) development of new FUV spectrometers, (4) FUV studies of liquid water and aqueous solutions, (5) FUV spectra of organic molecules in the liquid states, (6) band assignments by quantum chemical calculations, (7) potential applications of FUV spectroscopy in liquid and solid states; and (8) future prospects of FUV spectroscopy. © 2012 Society for Applied Spectroscopy.


Morisawa Y.,Kwansei Gakuin University | Morisawa Y.,Kinki University | Higashi N.,Kurabo Industries Ltd. | Takaba K.,Kwansei Gakuin University | And 4 more authors.
Review of Scientific Instruments | Year: 2012

A far-ultraviolet transient absorption spectrometer based on time-resolved attenuated total reflectance (ATR) has been developed and tested for aqueous solutions of phenol and tryptophan in the region 170-185 nm. In this region, a stable tunable laser was not available, and therefore, white light from a laser-driven Xe lamp source was used. The time resolution, which was determined by the time response of a continuous light detector, was 40 ns. A new ATR cell where a sample liquid is exchanged continuously by a flow system was designed to reduce efficiently the stray light from the excitation light. We have tested the performance of the instrument by using aqueous solutions of phenol and tryptophan, whose photochemistry is already well known. Phenol and tryptophan have very strong absorptions due to a π-π transition near 180 nm. Even for dilute solutions (10 -3 mol dm -3), we could observe decreases in their concentrations due to photochemistry that occurred upon their irradiation with a fourth harmonic generation laser pulse produced by an Nd:YAG laser. The sensitivity of the spectrometer was about 10 -4 abs, which corresponded to a concentration variation of 10 -3 mol dm -3 for phenol and tryptophan. © 2012 American Institute of Physics.


Goto T.,Japan National Agriculture and Food Research Organization | Ikehata A.,Japan National Agriculture and Food Research Organization | Morisawa Y.,Kwansei Gakuin University | Morisawa Y.,Kinki University | And 2 more authors.
Inorganic Chemistry | Year: 2012

The effects of the lanthanoid cations (Ln3+) on the first electronic transition (à ← X̃) of liquid water were studied from the attenuated total reflection far-ultraviolet (ATR-FUV) spectra of trivalent Ln3+ electrolyte solutions (1 M), except Pm3+. The à ← X̃ transition energies of the Ln3+ electrolyte solutions show a distinct tetrad in their dependence on the number of 4f electrons of the Ln3+ cations. For the half occupation period of the 4f electrons, the à ← X̃ transition energies decrease from La3+ (4f0, 8.0375 eV) to Nd3+ (4f3, 8.0277 eV) and increase from Sm3+ (4f5, 8.0279 eV) to Gd3+ (4f7, 8.0374 eV). For the complete occupation period, there are two local minima at Dy3+ (4f9, 8.0349 eV) and Yb3+ (4f13, 8.0355 eV). The à ← X̃ transition energies of the tetrad nodes (La3+, Gd3+, Ho3+ (4f 10), and Lu3+ (4f14)) increase slightly, as the nuclear charge increases in accordance with the hydration energies of the Ln3+ cations. The energy difference (δE) between the à ← X̃ transition energies and the line between La3+ and Lu3+ is largest at Nd3+ (80.5 cm-1) for the half occupation period and at Dy3+ (26.1 cm-1) and Yb 3+ (24.5 cm-1) for the complete occupation period. The order of magnitude of δE is comparable to the ligand field splitting (LFS) of the ground state multiplets of Ln3+ complexes. The observed tetrad trend of the à ← X̃ transition energies of the Ln 3+ electrolyte solutions across the 4f period reflects the hydration energies of the Ln3+ cations and the LFS induced by water ligands. © 2012 American Chemical Society.


Naitoh H.,Shiga University of Medical Science | Naitoh H.,Hino Memorial Hospital | Yamamoto H.,Shiga University of Medical Science | Murata S.,Shiga University of Medical Science | And 3 more authors.
Gastric Cancer | Year: 2014

Background: We conducted a multicenter phase II trial to assess the suitability of three types of chemotherapy (docetaxel plus S-1, irinotecan plus S-1, or S-1 alone) for patients with advanced gastric cancer by means of the collagen gel droplet embedded culture-drug sensitivity test (CD-DST). To our knowledge, this is the first multicenter clinical trial that has employed CD-DST to choose anticancer agents for the treatment of advanced gastric cancer.Methods: Subjects (n = 64) were patients with advanced or recurrent gastric cancer. Patients were allocated to one of the treatment regimens on the basis of CD-DST results. Outcome of the patients was compared between the groups deemed chemosensitive or chemoresistant by the CD-DST.Results: Thirty-three patients showed high sensitivity (T/C ratio <60 %) to at least one type of anticancer agent (sensitive group), and 31 showed low sensitivity (T/C ratio ≥60 %) to all agents (resistant group). Specifically, the 1-year survival rate was significantly higher in the sensitive group (78.5 %; 95 % CI, 67.2–94.7 %) than in the resistant group (54.7 %; 95 % CI, 38.7–74.3 %; P = 0.019), whereas time to progression (TTP) was significantly longer in the sensitive group (59.8 %; 95 % CI, 48.2–81.7 %) than in the resistant group (30.0 %; 95 % CI 13.6–46.4 %; P = 0.023). Median survival time was also significantly longer in the sensitive group (15.5 months; 95 % CI, 12.8–18.2) than in the resistant group (12.5 months; 95 % CI, 10.2–14.9; P = 0.038).Conclusions: CD-DST predicts the outcome of patients undergoing chemotherapy for advanced gastric cancer, presumably through evaluating chemosensitivity. © 2013, The Author(s).


PubMed | Tsukuba GeneTechnology Laboratories, Wagyu Registry Association, Kobe University, Kurabo Industries Ltd and Yamaguchi Prefectural Agriculture & Forestry General Technology Center
Type: Journal Article | Journal: Animal science journal = Nihon chikusan Gakkaiho | Year: 2016

In this study, we genotyped 117 autosomal single nucleotide polymorphisms using a DigiTag2 assay to assess the genetic diversity, structure and relationships of 16 Eurasian cattle populations, including nine cattle breeds and seven native cattle. Phylogenetic and principal component analyses showed that Bos taurus and Bos indicus populations were clearly distinguished, whereas Japanese Shorthorn and Japanese Polled clustered with European populations. Furthermore, STRUCTURE analysis demonstrated the distinct separation between Bos taurus and Bos indicus (K=2), and between European and Asian populations (K=3). In addition, Japanese Holstein exhibited an admixture pattern with Asian and European cattle (K=3-5). Mongolian (K=13-16) and Japanese Black (K=14-16) populations exhibited admixture patterns with different ancestries. Bos indicus populations exhibited a uniform genetic structure at K=2-11, thereby suggesting that there are close genetic relationships among Bos indicus populations. However, the Bhutan and Bangladesh populations formed a cluster distinct from the other Bos indicus populations at K=12-16. In conclusion, our study could sufficiently explain the genetic construction of Asian cattle populations, including: (i) the close genetic relationships among Bos indicus populations; (ii) the genetic influences of European breeds on Japanese breeds; (iii) the genetic admixture in Japanese Holstein, Mongolian and Japanese Black cattle; and (iv) the genetic subpopulations in Southeast Asia.


Tomioka T.,Kansai University | Maruoka H.,Kansai University | Maruoka H.,Kurabo Industries Ltd. | Kawa H.,Kansai University | And 4 more authors.
Neuroscience Research | Year: 2014

Histone deacetylase (HDAC) inhibitors induce histone acetylation and gene expression by changing local chromatin structures. They can thereby influence various cells to proliferate or differentiate. It has been reported that trichostatin A (TSA) or valproic acid (VPA) can induce the neuronal differentiation of mouse embryonic neural stem cells and rat cerebellar granule cells. It is unclear however which gene is responsible for the neuronal differentiation induced by HDAC inhibitors. In this study, we investigated the contribution of immediate early gene (IEG) nur77 to the neuronal differentiation induced by TSA. We report that TSA induces neurite outgrowth in PC12 cells, and C646, an inhibitor of HAT (histone acetyl transferase) (p300), prevents TSA-induced neurite formation. The acetylation of the Lys14 residue of histone H3, and mRNA and protein expression of nur77 gene were found to be stimulated after treatment with TSA, but not in the presence of C646. A knock-down of nur77 inhibits the neurite outgrowth induced by TSA. Furthermore, the ectopic expression of nur77 significantly elicits neurite formation in PC12 cells. These results suggest that the expression of nur77, which is up-regulated via the TSA-induced acetylation of Lys14 on histone H3, is essential for the neuronal differentiation in TSA-induced PC12 cells. © 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society.


Umemura K.,Kyoto University | Mihara A.,Kurabo Industries Ltd. | Kawai S.,Kyoto University
Journal of Wood Science | Year: 2010

The effects of a Maillard reaction between glucose and chitosan on the resultant chitosan films and the bonding properties of chitosans with different molecular weights were investigated. In film preparation, chitosan and glucose were dissolved in 1% acetic acid and dried in a Petri dish at 50°C. The bonding properties of the Maillard-reacted chitosan in three-ply plywood were evaluated by a tensile shear test. The weight, color, free amino groups, insoluble fraction, and thermal properties of the film changed significantly as the amount of added glucose increased. However, few differences in these properties due to the use of chitosan of different molecular weights were observed. The effect of glucose addition on the tensile strength of the film differed for chitosans of different molecular weights. Improvement of the strength by 10 wt% glucose addition was observed in low-molecular-weight chitosans. The dry- and wet-bond strengths were significantly enhanced with increasing glucose addition for low-molecular-weight chitosans. In addition, good bond strength was maintained even in 1% acetic acid solution. It was thus clarified that the bonding properties of low-molecular-weight chitosan were improved markedly by the environmentally safe method of glucose addition. © 2010 The Japan Wood Research Society.

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