He Y.,Kunming Medical University
Journal of Occupational and Environmental Medicine | Year: 2017
OBJECTIVES:: This study investigated whether variations in GSTT1 and GSTM1 gene are associated with the DNA damage level of p53 gene. METHODS:: We quantified urinary 1-hydroxypyrene using high-performance liquid chromatography, and examined the DNA damage level of p53 gene by real-time quantitative PCR in 756 coke-oven workers. Multiplex PCR was used to detect the presence or absence of genes. RESULTS:: DNA damage levels of p53 gene in the high exposure group and intermediate exposure group were significantly higher than that of p53 gene in the low exposure group (P?0.01). In coke-oven workers, the DNA damage levels of subjects with non-null genotype in GSTT1 or GSTM1 gene were significantly higher than that of those with the null genotype (P?0.01). CONCLUSIONS:: GSTT1 and GSTM1 may modulate DNA damage levels of p53 gene when exposed to polycyclic aromatic hydrocarbons. Copyright © 2017 by the American College of Occupational and Environmental Medicine
Wang Y.,Kunming Medical University
Spine | Year: 2017
STUDY DESIGN.: Retrospective study. OBJECTIVE.: To analyze the intraoperative neuromonitoring (IOM) changes in posterior vertebral column resection (PVCR) for severe rigid deformity patients, and describe our stepwise responding strategies. SUMMARY OF BACKGROUND DATA.: Obvious neurological deficit risk accompanied with PVCR correction has been emphasized repeatedly. METHODS.: The records of 46 PVCR patients achieved IOM were reviewed. IOM alerts triggered responding protocols: 1) exchange the convex corrective rod to concave stabilizing rod, 2) appropriate compression for spinal shortening, 3) reversed in-situ rod bending, 4) translation technique and uni-segmental derotation, 5) adjacent segmental resection. RESULTS.: The overall scoliotic correction rate was 65.4% (from 112?±?28.6 to 39?±?13.4) and segmental kyphotic 64.2% (from 101?±?37.3 to 36?±?19.2). During correction step, SSEP warning (3) and SSEP/TcMEP warning (8) were detected in 11 patients (23.9%). Probable cause identification including rule out IOM technical factors, residual impingement, and if there was unstable spinal column (1), spinal cord excessive tension on concave side (3), and the excessive opposite spinal displacement between two aspects of resected area (7). After rod change (1), compression (2), bending (3), derotation (3) and adjacent resection (2), all IOM changes went to under warning criteria. All 11 patients revealed neurologically intact postoperatively. There was no difference of correction rate between IOMs alert or not. However, adult, extremity severe or sharp angular curves trend to more common in IOM alert patients. CONCLUSION.: As 3-dimensional spinal column divided and re-linked in PVCR, and the correction maneuvers were restricted on single dimension, inevitably resulted in spinal cord tension changes and spinal column opposite displacement. To timely identify them and prompt interventions should be performed, and even enlarge the resected area to reduce the abrupt turning tendency of the spinal cord.Level of Evidence: 5 Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.
Zhang M.,Michigan State University |
Zhang M.,Kunming Medical University |
Wang H.,Michigan State University
Learning and Memory | Year: 2013
There is significant interest in understanding the contribution of intracellular signaling and synaptic substrates to memory flexibility, which involves new learning and suppression of obsolete memory. Here, we report that enhancement of Ca2+-stimulated cAMP signaling by overexpressing type 1 adenylyl cyclase (AC1) facilitated long-term potentiation (LTP) but impaired long-term depression (LTD) at the hippocampal Shaffer collateral-CA1 synapses. However, following the induction of LTP, low-frequency stimulation caused comparable synaptic depotentiation in both wild type and AC1 transgenic (AC1 TG) mice. Although previous studies have suggested the function of LTD in spatial memory flexibility, AC1 TG mice showed not only better initial learning in the Morris water maze, but also faster acquisition and increased ratio of new memory formation to old memory retention during the reversal platform training. In the memory extinction test, which requires suppression of old memory without involving the acquisition of the new platform information, AC1 TG and wild type mice showed comparable performance. Our results demonstrate new functions of Ca2+-stimulated AC1, and also suggest that certain aspects of hippocampus-dependent behavioral flexibility may not require intact LTD.© 2013, Published by Cold Spring Harbor Laboratory Press.
Zhong J.-H.,Guangxi Medical University |
Ke Y.,Guangxi Medical University |
Ke Y.,Kunming Medical University |
Wang Y.-Y.,Guangxi Medical University |
Li L.-Q.,Guangxi Medical University
Gut | Year: 2015
Sir, We read with great interest the leading article by Bruix et al1 published in Gut. This article recommended palliative treatments for patients with hepatocellular carcinoma (HCC) involving macrovascular invasion, multiple tumours, or portal hypertension. With better patient selection and improvement of perioperative care, liver resection (LR) offers the most consistent and clinically meaningful long-term survival in HCC over the past 20 years, which has been documented by both Eastern and Western centres.23 However, Western official guidelines do not recommend LR for treating intermediate and advanced stage HCC.45 Here, we systematically searched PubMed database for studies investigating the safety and efficacy of LR for treating patients with HCC involving macrovascular invasion, multiple tumours (≥2) or portal hypertension. We only included studies which were published in English on or after January 2000. In the case of multiple studies based on the same patients, we selected the study with the largest sample size.
Yang Z.,Kunming Medical University
Cell Death and Differentiation | Year: 2016
Necroptosis is a caspase-independent form of regulated cell death executed by the receptor-interacting protein kinase 1 (RIP1), RIP3, and mixed lineage kinase domain-like protein (MLKL). Recently, necroptosis-based cancer therapy has been proposed to be a novel strategy for antitumor treatment. However, a big controversy exists on whether this type of therapy is feasible or just a conceptual model. Proponents believe that because necroptosis and apoptosis use distinct molecular pathways, triggering necroptosis could be an alternative way to eradicate apoptosis-resistant cancer cells. This hypothesis has been preliminarily validated by recent studies. However, some skeptics doubt this strategy because of the intrinsic or acquired defects of necroptotic machinery observed in many cancer cells. Moreover, two other concerns are whether or not necroptosis inducers are selective in killing cancer cells without disturbing the normal cells and whether it will lead to inflammatory diseases. In this review, we summarize current studies surrounding this controversy on necroptosis-based antitumor research and discuss the advantages, potential issues, and countermeasures of this novel therapy.Cell Death and Differentiation advance online publication, 26 February 2016; doi:10.1038/cdd.2016.8. © 2016 Macmillan Publishers Limited
Zhong H.,Kunming Medical University
Investigative ophthalmology & visual science | Year: 2012
We evaluated the prevalence of glaucoma in adults of the Bai Nationality populations in rural China. A population-based survey of Chinese Bai Nationality aged ≥50 years from randomly selected block groups in southwestern China was conducted. Eligible persons were invited to local examination sites for a complete ophthalmic examination. Glaucoma was diagnosed using the International Society of Geographical and Epidemiological Ophthalmology Classification scheme. In the study, 2133 subjects (77.8% participation rate) were examined, with a crude prevalence of all glaucoma of 2.2% (95% confidence interval [CI] 1.6%-2.9%). Primary open angle glaucoma (POAG) was found in 1.0% of cases (95% CI 0.6%-1.6%) and primary angle-closure glaucoma (PACG) in 0.9% (95% CI 0.6%-1.4%). The prevalence of all glaucoma was significantly higher in older people and women. Mean intraocular pressure (IOP) was 16.17 ± 3.74 mm Hg (97.5th and 99.5th percentiles, 24 mm Hg and 30 mm Hg, respectively). The mean vertical cup-to-disc ratio (VCDR) was 0.43 ± 0.17 (97.5th and 99.5th percentiles 0.7 and 0.8, respectively). Unilateral blindness was found in 80% of PACG, compared to only 36.3% of POAG cases. Prevalence of POAG is similar to PACG in the ethnic Bai population living in rural southwestern China. PACG has a worse visual impairment and prognosis compared to POAG.
Wang Y.,Kunming Medical University
Journal of Spinal Disorders and Techniques | Year: 2015
STUDY DESIGN:: A retrospective study.OBJECTIVE:: To investigate whether PJK or obvious proximal junctional angle (PJA) changes in the sagittal plane develops following short fusion in children younger than 10 years of age with congenital scoliosis, and to investigate the possible risk factors.SUMMARY OF BACKGROUND DATA:: Proximal junctional kyphosis (PJK) following long spinal fusion in adolescents and adults is a serious postoperative complication. Although the same problem may occur patients with early onset scoliosis (EOS) that have undergone spine fusion, few studies have been reported any relationship between PJK and spinal fusion in young children with congenital scoliosis.METHODS:: Thirty-seven children treated in a single institution between 1998 and 2010 were reviewed retrospectively. The inclusion criteria included (1) <10 years of age at the time of operation; (2) simple congenital deformity; (3) hemivertebra treated by posterior hemivertebrectomy with short fusion at a maximum of 5 motion segments; (4) minimum follow-up two years. The PJA from the caudal endplate of the upper instrumentation vertebral (UIV) to the cephalad endplate of the vertebra adjacent to the UIV, thoracic kyphosis (T5-T12), lumbar lordosis (T12-S1), global sagittal balance, and magnitude of scoliosis of the major curves and upper compensated curves were measured on lateral radiographs. PJK was defined by a PJA greater than 10° during the follow-up and at least 10° greater than the preoperative or early postoperative measurement. Wilcoxon tests were performed for statistical analysis.RESULTS:: PJK occurred in 7 out of 37 patients (18.9%), during an average of 4.5±3.2 years follow-up (2-12 y). The UIV level of children with PJK was on T9 in four patients, and T11, T12, and L1 in one. Screw malposition at UIV was confirmed by postoperative CT images in six patients. Only one patient with a screw deviation did not develop PJK during the follow-up period. None of the patients with PJK was symptomatic, and no patients required revision surgery because of PJK. PJK occurred and progressed during the first six months after surgery followed by almost no progression or slight improvement in patients that could be followed up beyond 6 months postoperatively; in association with an increase of the lumbar lordosis.CONCLUSIONS:: PJK occurred in pediatric patients with simple congenital deformities following hemivertebrectomy and short fusion. PJK was more common in patients with (1) greater immediately postoperative segmental kyphosis and proximal junctional angle, (2) screw malposition on the UIV, and (3) hemivertebra located on the lower thoracic or the thoracolumbar region. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
Xiong P.,Kunming Medical University
The Journal of clinical psychiatry | Year: 2014
It is difficult for clinicians to diagnose schizophrenia solely based on interviews. We explored the diagnostic efficiency and predictive capability of serum biomarkers for schizophrenia. Levels of β nerve growth factor (β-NGF), brain-derived neurotrophic factor (BDNF), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), interferon γ (IFN-γ), calcium binding protein S100β, myelin basic protein (MBP), and glial fibrillary acidic protein (GFAP) were measured in the sera of 278 schizophrenia patients, 240 depression and bipolar disorder patients, and 260 healthy controls. DSM-IV-TR criteria were used as the diagnostic criteria for schizophrenia and depressive and bipolar disorders. The diagnostic efficiency was high in patients with schizophrenia compared with the healthy controls. Receiver operating characteristic (ROC) curve analysis was used to ascertain the diagnostic efficiency of the 8 proteins. Data were collected between July 2010 and December 2012. One-way analysis of variance significantly demonstrated lower serum BDNF, MBP, and GFAP levels (F = 16.504, P < .001; F = 207.209, P < .001; F = 33.668, P < .001, respectively) but higher serum IL-6 and S100β concentrations (F = 15.250, P < .001; F = 12.751, P < .001, respectively) among patients with schizophrenia. ROC analysis of the discriminant scores of the serum β-NGF, BDNF, IL-6, S100β, MBP, and GFAP levels resulted in significant discrimination between the schizophrenia and control groups (AUC = 0.922) and the depressive/bipolar disorder and control groups (AUC = 0.762). Serum levels of 6 proteins (but not TNF-α and IFN-γ) contribute most to the diagnosis of schizophrenia. These proteins may prove to be useful adjuncts for the clinical assessment of this disease. © Copyright 2014 Physicians Postgraduate Press, Inc.
Yang C.-R.,Kunming Medical University
Neuropsychopharmacology | Year: 2016
Chronic exposure to stressful environment is a key risk factor contributing to the development of depression. However, the mechanisms involved in this process are still unclear. Brain-derived neurotropic factor (BDNF) has long been investigated for its positive role in regulation of mood, although the role of its precursor, proBDNF, in regulation of mood is not known. In this study, using an unpredictable chronic mild stress (UCMS) paradigm we found that the protein levels of proBDNF were increased in the neocortex and hippocampus of stressed mice and this UCMS-induced upregulation of proBDNF was abolished by chronic administration of fluoxetine. We then established a rat model of UCMS and found that the expression of proBDNF/p75NTR/sortilin was upregulated, whereas the expression of mature BDNF and TrkB was downregulated in both neocortex and hippocampus of chronically stressed rats. Finally, we found that the injection of anti-proBDNF antibody via intracerebroventricular (i.c.v.) and intraperitoneal (i.p.) approaches into the UCMS rats significantly reversed the stress-induced depression-like behavior and restored the exploratory activity and spine growth. Although intramuscular injection of AAV-proBDNF did not exacerbate the UCMS-elicited rat mood-related behavioral or pathological abnormalities, i.c.v. injection of AAV-proBDNF increased the depression-like behavior in naive rats. Our findings suggest that proBDNF plays a role in the development of chronic stress-induced mood disturbances in rodents. Central (i.c.v.) or peripheral (i.p.) inhibition of proBDNF by injecting specific anti-proBDNF antibodies may provide a novel therapeutic approach for the treatment of stress-related mood disorders.Neuropsychopharmacology advance online publication, 13 July 2016; doi:10.1038/npp.2016.100. © 2016 American College of Neuropsychopharmacology