Li Y.,Kunming Medical University
Cancer Research and Clinic | Year: 2014
Objective: To determine the relationship between CCDC8 gene and breast cancer. Methods: 40 cancerous breast tissue and 22 benign breast tissue were included. qRT-PCR was performed to investigate the expression level of CCDC8 in breast tissue. The correlation between CCDC8 level and the age of patients, tumor size, clinical staging, and the expression levels of estrogen and progesterone receptors, CerbB2, Ki-67, p53 and nm23 were analyzed. Results: The expression level of CCDC8 in benign breast tissue (1685±755) was significantly higher than that in cancerous tissues (502.1±223.2). Tissues obtained from patients over age 50 showed an increased level of CCDC8 (789.8±367) in comparison to those from patients age 50 or younger (452.5±170.3). The level of CCDC8 expression was negatively correlated with nm23 level (Correlation Coefficient = -0.400, P = 0.039), while no correlation was found between CCDC8 and cancer stages, estrogen and progesterone receptor, CerbB2, Ki-67and p53. Conclusion: The negative correlations between CCDC8 and age, tumor size and nm23 indicate that CCDC8 is a potential tumor suppressor, influencing the occurrence and progression in breast cancer.
Wang Y.,Kunming Medical University
Journal of Spinal Disorders and Techniques | Year: 2015
STUDY DESIGN:: A retrospective study.OBJECTIVE:: To investigate whether PJK or obvious proximal junctional angle (PJA) changes in the sagittal plane develops following short fusion in children younger than 10 years of age with congenital scoliosis, and to investigate the possible risk factors.SUMMARY OF BACKGROUND DATA:: Proximal junctional kyphosis (PJK) following long spinal fusion in adolescents and adults is a serious postoperative complication. Although the same problem may occur patients with early onset scoliosis (EOS) that have undergone spine fusion, few studies have been reported any relationship between PJK and spinal fusion in young children with congenital scoliosis.METHODS:: Thirty-seven children treated in a single institution between 1998 and 2010 were reviewed retrospectively. The inclusion criteria included (1) <10 years of age at the time of operation; (2) simple congenital deformity; (3) hemivertebra treated by posterior hemivertebrectomy with short fusion at a maximum of 5 motion segments; (4) minimum follow-up two years. The PJA from the caudal endplate of the upper instrumentation vertebral (UIV) to the cephalad endplate of the vertebra adjacent to the UIV, thoracic kyphosis (T5-T12), lumbar lordosis (T12-S1), global sagittal balance, and magnitude of scoliosis of the major curves and upper compensated curves were measured on lateral radiographs. PJK was defined by a PJA greater than 10° during the follow-up and at least 10° greater than the preoperative or early postoperative measurement. Wilcoxon tests were performed for statistical analysis.RESULTS:: PJK occurred in 7 out of 37 patients (18.9%), during an average of 4.5±3.2 years follow-up (2-12 y). The UIV level of children with PJK was on T9 in four patients, and T11, T12, and L1 in one. Screw malposition at UIV was confirmed by postoperative CT images in six patients. Only one patient with a screw deviation did not develop PJK during the follow-up period. None of the patients with PJK was symptomatic, and no patients required revision surgery because of PJK. PJK occurred and progressed during the first six months after surgery followed by almost no progression or slight improvement in patients that could be followed up beyond 6 months postoperatively; in association with an increase of the lumbar lordosis.CONCLUSIONS:: PJK occurred in pediatric patients with simple congenital deformities following hemivertebrectomy and short fusion. PJK was more common in patients with (1) greater immediately postoperative segmental kyphosis and proximal junctional angle, (2) screw malposition on the UIV, and (3) hemivertebra located on the lower thoracic or the thoracolumbar region. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
Zhong H.,Kunming Medical University
Investigative ophthalmology & visual science | Year: 2012
We evaluated the prevalence of glaucoma in adults of the Bai Nationality populations in rural China. A population-based survey of Chinese Bai Nationality aged ≥50 years from randomly selected block groups in southwestern China was conducted. Eligible persons were invited to local examination sites for a complete ophthalmic examination. Glaucoma was diagnosed using the International Society of Geographical and Epidemiological Ophthalmology Classification scheme. In the study, 2133 subjects (77.8% participation rate) were examined, with a crude prevalence of all glaucoma of 2.2% (95% confidence interval [CI] 1.6%-2.9%). Primary open angle glaucoma (POAG) was found in 1.0% of cases (95% CI 0.6%-1.6%) and primary angle-closure glaucoma (PACG) in 0.9% (95% CI 0.6%-1.4%). The prevalence of all glaucoma was significantly higher in older people and women. Mean intraocular pressure (IOP) was 16.17 ± 3.74 mm Hg (97.5th and 99.5th percentiles, 24 mm Hg and 30 mm Hg, respectively). The mean vertical cup-to-disc ratio (VCDR) was 0.43 ± 0.17 (97.5th and 99.5th percentiles 0.7 and 0.8, respectively). Unilateral blindness was found in 80% of PACG, compared to only 36.3% of POAG cases. Prevalence of POAG is similar to PACG in the ethnic Bai population living in rural southwestern China. PACG has a worse visual impairment and prognosis compared to POAG.
Tai W.L.,Kunming Medical University
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2012
To investigate the effect of intravenous bone marrow-derived mesenchymal stem cell (MSC) transplantation for early intervention of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Thirty-six mice were randomized into control group, PBS-treated ALI group, and MSC-treated ALI group. In the latter two groups, mouse models of ALI were established by intranasal instillation of LPS, and 1 h later, the 4th passage of MSCs isolated from the bone marrow of mice or PBS were administered via the tail vein. The histological findings, lung wet/dry (W/D) weight ratio, neutrophil count and protein and cytokine contents in the bronchoalveolar lavage fluid (BALF), and myeloperoxidase (MPO) level in the lung tissue were analyzed at 24 h after MSC administration. Engraftment of MSCs in the recipient lung was determined by fluorescent PKH26 staining and flow cytometry. Compared with the control group, PBS-treated ALI group showed significantly higher protein levels, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and neutrophil count in the BALF and MPO content in the lung tissue, with also severe damage of lung histology. MSCs administration significantly reduced the lung W/D weight ratio, the levels of protein, TNF-α, IL-6 and neutrophil count in the BALF and MPO content in the lung tissue, and obviously lessened the lung injury 24 h after the transplantation. MSC administration also significantly increased the level of IL-10 in the BALF. Intravenous MSC transplantation can effectively improve the lung histology, attenuate the inflammatory response, reduce pulmonary edema in the early stage of LPS-induced ALI in mice, and such effects are independent of MSC engraftment in the lungs.
Yang Z.,Kunming Medical University
Cell Death and Differentiation | Year: 2016
Necroptosis is a caspase-independent form of regulated cell death executed by the receptor-interacting protein kinase 1 (RIP1), RIP3, and mixed lineage kinase domain-like protein (MLKL). Recently, necroptosis-based cancer therapy has been proposed to be a novel strategy for antitumor treatment. However, a big controversy exists on whether this type of therapy is feasible or just a conceptual model. Proponents believe that because necroptosis and apoptosis use distinct molecular pathways, triggering necroptosis could be an alternative way to eradicate apoptosis-resistant cancer cells. This hypothesis has been preliminarily validated by recent studies. However, some skeptics doubt this strategy because of the intrinsic or acquired defects of necroptotic machinery observed in many cancer cells. Moreover, two other concerns are whether or not necroptosis inducers are selective in killing cancer cells without disturbing the normal cells and whether it will lead to inflammatory diseases. In this review, we summarize current studies surrounding this controversy on necroptosis-based antitumor research and discuss the advantages, potential issues, and countermeasures of this novel therapy.Cell Death and Differentiation advance online publication, 26 February 2016; doi:10.1038/cdd.2016.8. © 2016 Macmillan Publishers Limited