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Huang L.,PLA Fourth Military Medical University | Sun X.,PLA Fourth Military Medical University | Xiao Y.-H.,Kunming General Hospital of PLA | Dong Y.,PLA Fourth Military Medical University | And 5 more authors.
Journal of Biomedical Materials Research - Part B Applied Biomaterials

The antibacterial properties of resins incorporating MAE-DB and the underlying mechanisms of action were evaluated. Antibacterial effects against Streptococcus mutans were tested using the film contact method, with accumulation and membrane integrity observed by scanning electron microscopy and confocal laser scanning microscopy, respectively. Quantitative PCR was used to determine expression of the S. mutans glucosyltransferase B (gtfB) gene on the surface of resins containing 10% MAE-DB. Bacterial growth was inhibited on resin containing 10% MAE-DB as compared with the control at 1 day, 7 days, 30 days, or 180 days (p < 0.05). For the 10%-MAE-DB resin, no significant differences in bacterial viability were found regardless of the time of incubation (p > 0.05). The number of bacteria attached to resin containing 10% MAE-DB was considerably lower than the control. The proportion of bacteria with damaged cell membranes was increased in the experimental resin over controls. Expression of gtfB was reduced by 10% MAE-DB compared with the control (p < 0.05). These findings demonstrate that MAE-DB can be incorporated into resin materials at sufficient concentrations for long-term antibacterial effects against S. mutans after polymerization by attenuating gtfB expression and impairing membrane integrity. © 2012 Wiley Periodicals, Inc. Source

Deng J.-W.,No.535 Hospital of PLA | Yang Y.-T.,Heze Municipal Hospital | Zeng Y.,Kunming General Hospital of PLA | Tang Z.-M.,No.535 Hospital of PLA | And 2 more authors.
Journal of Cataract and Refractive Surgery

Nucleus extraction in manual sutureless extracapsular cataract extraction (ECCE) using the 2-hook technique is described. After capsulorhexis and hydrodissection are performed, the nucleus is moved into the anterior chamber and extracted by pulling with a Sinskey hook and pressuring the scleral bed with a Kuglen hook. In a series of 1320 eyes, 85% achieved a corrected visual acuity of 5/10 or better postoperatively. Complications were posterior capsule rupture, vitreous loss, and transient corneal edema. Manual sutureless ECCE using the 2-hook technique is safe and efficient and does not require expensive instrumentation. Financial Disclosure: No author has a financial or proprietary interest in any material or method mentioned. © 2013 ASCRS and ESCRS. Source

Gui Q.,Hunan University | Yang Z.,Hunan University | Chen X.,Hunan University | Liu J.,Hunan University | And 3 more authors.

Synthesis of phenanthridin-6(5H)-ones was achieved via copper-catalyzed cyclization of 2-phenylbenzamides using air as the oxidant and KOt-Bu as the base. It was discovered that, besides Ph3P, other ligands such as 1,10-phenanthroline, TMEDA as well as l-proline could also be used as the ligand to effect the transformation. © Georg Thieme Verlag Stuttgart. New York. Source

Sai M.,PLA Fourth Military Medical University | Le-Qun S.,PLA Fourth Military Medical University | Yu-Hong X.,Kunming General Hospital of PLA | Fang L.,PLA Fourth Military Medical University | And 3 more authors.
Brazilian Journal of Medical and Biological Research

Antibacterial monomers incorporated in dentin bonding systems may have toxic effects on the pulp. Thus, the cytotoxicity of antibacterial monomers and its underlying mechanisms must be elucidated to improve the safety of antibacterial monomer application. The influence of an antibacterial monomer, methacryloxylethyl cetyl ammonium chloride (DMAE-CB), on the vitality of L929 mouse fibroblasts was tested using MTT assay. Cell cycle progression was studied using flow cytometry. Production of intracellular reactive oxygen species (ROS) after DMAE-CB treatment was measured using 2,7-dichlorodihydrofluorescein diacetate staining and flow cytometry analysis. Loss of mitochondrial membrane potential, disturbance of Bcl-2 and Bax expression, as well as release of cytochrome C were also measured using flow cytometry analysis or Western blot to explore the possible involvement of the mitochondrial-related apoptotic pathway. DMAE-CB elicited cell death in a dose-dependent manner and more than 50% of cells were killed after treatment with 30 μM of the monomer. Both necrosis and apoptosis were observed. DMAE-CB also induced G1- and G2-phase arrest. Increased levels of intracellular ROS were observed after 1 h and this overproduction was further enhanced by 6-h treatment with the monomer. DMAE-CB may cause apoptosis by disturbing the expression of Bcl-2 and Bax, reducing the mitochondrial potential and inducing release of cytochrome C. Taken together, these findings suggest that the toxicity of the antibacterial monomer DMAE-CB is associated with ROS production, mitochondrial dysfunction, cell cycle disturbance, and cell apoptosis/necrosis. Source

Li G.-H.,Chongqing Medical University | Wei H.,Chongqing Medical University | Lv S.-Q.,Chongqing Medical University | Ji H.,Kunming General Hospital of PLA | Wang D.-L.,Chongqing Medical University
International Journal of Oncology

Glioblastoma is a highly lethal brain tumor of the human primary nervous system tumors. Previous studies demonstrated that glioblastoma stem cells were able to initiate and reform the original cancer. In this study, we found that there were expression and activation of STAT3, a key signal transduction factor and oncoprotein, in human glioblastoma stem cells (GSCs). STAT3 plays a key role in proliferation, apoptosis and differentiation in embryonic stem cells and several cancer types. To investigate the effects of STAT3 on human GSCs, the expression and activation of STAT3 were suppressed by RNAi mediated with lentivirus. We demonstrated that siRNA of STAT3 significantly suppressed STAT3 expression and activation and resulted in inhibition of cell growth in GSCs. Knockdown of STAT3 induces apoptosis and reduces significantly expression of Bcl-2 and cyclin-D in human primary GSCs, whereas no significance was achieved in BAX and caspase-3 expression. Inhibition of STAT3 expression is associated not only with decreasing of CD133+ cell proportion and increasing of GFAP and MBP exression, but also with decrease of the capacity to initiate a tumor in human primary GSCs. Together, these studies suggest that STAT3 is an important target for human GSCs in regulation of GSCs growth, apoptosis, differentiation and tumorigenic potential. Source

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