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Pang R.,Sun Yat Sen University | Pang R.,Kunming general hospital of Peoples Liberation Army | Zhang Y.,Yunnan Agricultural University | Pan X.,Kunming general hospital of Peoples Liberation Army | And 8 more authors.
Cellular and Molecular Biology | Year: 2010

Embryonic-like stem cell (ELSC), expressing part of surface markers of human embryonic stem cells, may be a better candidate for cell therapy of degenerative muscular disease than mesenchymal stem cell (MSC). We isolated ELSC and MSC from bone marrow, respectively, and compared their differences in the characteristics and the capacity of myogenic differentiation. Results showed that ELSC could be isolated successfully from 3 adult bone marrow samples by using serum-free medium with 10ng/ml basic fibroblast growth factor (bFGF). At the same cell density, MSC could also be isolated from the same samples by using DMEM/F12 medium containing 10% new cattle serum. However, ELSC appeared as small, morphologically slenderer, upregulated expression of SSEA-4 and ultramicroscopically more immature than MSC derived from the same samples. Immunofluorescent staining and RT-PCR analysis showed ELSC weakly expressed Oct-4, Nanog-3 and Sox-2. Moreover, ELSC and MSC could be induced into long, multinucleated fibers expressing myogenin and myosin heavy chain (MHC) in myogenic differentiation medium, but by day 10, proportion of multinucleated fibers positive for MHC was respectively 25.0%±6.9% and 13.8%±7.6% in ELSC and MSC culture. These data suggest that bone marrow derived ELSC represent an ideal candidate for cell therapy of degenerative muscular disease. © 2010 C.M.B. Edition.


Liu Y.-S.,Kunming General Hospital of Peoples Liberation Army | Feng Y.-P.,Kunming General Hospital of Peoples Liberation Army | Xie J.-X.,Kunming General Hospital of Peoples Liberation Army | Luo Z.-J.,PLA Fourth Military Medical University | And 9 more authors.
PLoS ONE | Year: 2012

Effective immobilization and transportation are vital to the life-saving acute medical care needed when treating critically injured people. However, the most common types of stretchers used today are wrought with problems that can lead to further medical complications, difficulty in employment and rescue, and ineffective transitions to hospital treatment. Here we report a novel first aid stretcher called the "emergency carpet", which solves these problems with a unique design for spine injured patients. Polyurethane composite material, obtained by a novel process of manually mixing isocyanate and additives, can be poured into a specially designed fabric bag and allowed to harden to form a rigid human-shaped stretcher. The effectiveness of the emergency carpet was examined in the pre-hospital management of victims with spinal fractures. Additionally, it was tested on flat ground and complex terrain as well as in the sea and air. We demonstrated that the emergency carpet can be assembled and solidified on the scene in 5 minutes, providing effective immobilization to the entire injured body. With the protection of the emergency carpet, none of the 20 patients, who were finally confirmed to have spinal column fracture or dislocation, had any neurological deterioration during transportation. Furthermore, the carpet can be handled and transported by multiple means under differing conditions, without compromising immobilization. Finally, the emergency carpet allows the critically injured patient to receive multiple examinations such as X-ray, CT, and MRI without being removed from the carpet. Our results demonstrate that the emergency carpet has ideal capabilities for immobilization, extrication, and transportation of the spine injured patients. Compared with other stretchers, it allows for better mobility, effective immobilization, remarkable conformity to the body, and various means for transportation. The emergency carpet is promising for its intrinsic advantages in the pre-hospital management of accident victims. © 2012 Liu et al.


Peng L.-H.,Kunming General Hospital of Peoples Liberation Army | Hu D.-M.,Kunming General Hospital of Peoples Liberation Army | Wang S.-B.,Kunming General Hospital of Peoples Liberation Army
Journal of Leukemia and Lymphoma | Year: 2010

Objective: To retrospectively analyze the efficacy of a pediatric treatment protocol, BFM90, in adult patients with acute lymphoblastic leukemia (ALL) up to the age of 60 years. Methods: From August 2004 to October 2007, 60 adult patients (median age, 40 years; range, 18 to 60 years) with Philadelphia chromosome-negative ALL were treated with the BFM90 protocol. Clinical effect were historically compared with that of the 35 patients (median age, 42 years; range, 18 to 56 years) who were treated with Hyper-CVAD protocol. Results: At 42 months, complete remission (CR) rate, event-free survival (EFS) and overall survival (OS) rates were 93% (56 patients), 60% (36 patients) and 65% (39 patients), respectively. Age is an important prognostic factor, with 45 years of age as best cutoff. CR (P =0.02), OS (P <0.001), and EFS (P <0.001) of BFM90 were compared superiorly with that of the previous Hyper-CVAD experience. Conclusion: These results suggest that pediatric protocol superior to the outcome of adult patients with Philadelphia chromosome-negative ALL.


Zhou X.L.,Kunming General Hospital of Peoples Liberation Army
Zhonghua yi xue za zhi | Year: 2011

To summarize the clinical experiences of treating deep venous thrombosis (DVT) with retrievable vena cava filter. A total of 180 patients with DVT in unilateral lower extremity were recruited. There were 82 males and 98 females with an average age of 46 years old. After the diagnosis of vascular color Doppler ultrasound, retrievable vena cava filter was implanted through contralateral femoral vein. Simultaneously another 114 patients underwent femoral vein embolectomy. All cases received the post-operative therapies of anticoagulation, thrombolysis and vasodilation. The implantation of retrievable vena cava filter was successful in all cases. Among 142 cases of attempted extraction, 116 filters were extracted successfully at Days 12 - 24. The success rate was 81.69%. Filters captured thrombosis in 34 cases (29.31%). As a safe and efficacious method, the implantation of retrievable vena cava filter prevents the occurrence of pulmonary embolism in the patients with DVT in unilateral lower extremity. Its timely extraction may avoid the long-term complications.


PubMed | Kunming General Hospital of Peoples Liberation Army
Type: Clinical Trial | Journal: Zhonghua yi xue za zhi | Year: 2011

To summarize the clinical experiences of treating deep venous thrombosis (DVT) with retrievable vena cava filter.A total of 180 patients with DVT in unilateral lower extremity were recruited. There were 82 males and 98 females with an average age of 46 years old. After the diagnosis of vascular color Doppler ultrasound, retrievable vena cava filter was implanted through contralateral femoral vein. Simultaneously another 114 patients underwent femoral vein embolectomy. All cases received the post-operative therapies of anticoagulation, thrombolysis and vasodilation.The implantation of retrievable vena cava filter was successful in all cases. Among 142 cases of attempted extraction, 116 filters were extracted successfully at Days 12 - 24. The success rate was 81.69%. Filters captured thrombosis in 34 cases (29.31%).As a safe and efficacious method, the implantation of retrievable vena cava filter prevents the occurrence of pulmonary embolism in the patients with DVT in unilateral lower extremity. Its timely extraction may avoid the long-term complications.

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