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Zhou X.,Taizhou Central Hospital | Zhou X.,Shanghai JiaoTong University | Zhang C.,Kunhua Hospital of Kunming Medical College | Wang X.,Shanghai JiaoTong University | And 3 more authors.
Journal of Surgical Research | Year: 2012

Background: Wear particle-induced osteolysis could lead to the aseptic loosening of implants. Studies have suggested that endotoxins, such as lipopolysaccharides (LPS), may be the primary causes of wear particle-mediated osteolysis, and that osteolysis may originate from subclinical levels of bacterial infection. However, effective therapies against wear particles and gram-negative bacterial or LPS-induced bone resorption are limited. Materials and Methods: In the current study, the effect of berberine on LPS- and polyethylene (PE) particle-induced osteolysis in vivo was investigated using a mouse calvarial model. Osteoclast number per bone perimeter and eroded surface per bone surface were measured. Results: Berberine (10 mg/kg), injected either simultaneously with LPS or 3 d after LPS (25 mg/kg) treatment, blocked LPS-induced osteoclast recruitment and bone resorption in the mouse calvarial model. A daily single-dose of berberine (10 mg/kg), injected either simultaneously with PE particles or 4 d after treatment with PE particles, blocked PE particle-induced osteoclast recruitment and bone resorption. Berberine treatment markedly decreased LPS and PE particle-induced osteoclast recruitment and bone resorption in the murine calvarial model. Conclusion: These results suggest that berberine may have therapeutic effect for osteolysis induced by wear particles and LPS in gram-negative bacteria. © 2012 Elsevier Inc. All rights reserved.


Zhou X.,Shanghai JiaoTong University | Zhou X.,Taizhou Central Hospital | Zhang P.,Chinese University of Hong Kong | Zhang C.,Kunhua Hospital of Kunming Medical College | And 2 more authors.
Calcified Tissue International | Year: 2010

An experiment was designed to investigate whether systemic administration of tetracyclines (TCs) as bone fluorochrome labels could interfere with bone modeling in vivo and inhibit osteoclast formation and activity in vitro. Cell cultures of rat bone marrow macrophages revealed that TC and oxytetracycline inhibited osteoclastogenesis and bone resorption and stimulated apoptosis. Forty rats in five groups were treated with saline, calcein green, alizarin red S, TC, or oxytetracycline. Their tibias were used for histomorphometric analysis, including bone static, dynamic, and resorption parameters in the tibial proximal metaphysis. No significant differences in bone volume per tissue volume, trabecular number, trabecular thickness, trabecular separation, bone formation rate per bone surface, mineralizing surface, or mineral apposition rate were observed. TC or oxytetracycline decreased eroded surface, number of osteoclasts per bone perimeter, and osteoclast surface per bone surface by about 50%. The results demonstrated that TC and oxytetracycline inhibit rat osteoclast formation and activity in vitro, and histomorphometric parameters involved in bone turnover may be affected by the use of oxytetracycline and TC as fluorescent bone labels in vivo. © 2009 Springer Science+Business Media, LLC.


Zhou X.,Shanghai JiaoTong University | Zhou X.,Taizhou Central Hospital | Zhang P.,Chinese University of Hong Kong | Zhang C.,Kunhua Hospital of Kunming Medical College | Zhu Z.,Shanghai JiaoTong University
Journal of Orthopaedic Research | Year: 2010

Diabetic patients have an increased risk of prosthesis failure requiring revision surgery. Furthermore, skeletal defects are observed in conjunction with type 1 diabetes. Using a titanium particle-induced calvarial osteolysis model in diabetic mice, we investigated the effect of diabetes on the osteolytic process and the role of naringin in its prevention. Three groups each of nondiabetic or diabetic mice were treated with vehicle only, with particles only, or with particles then naringin for 10 days. Alteration of bone indices near the midline suture were then analyzed by microcomputed tomography scanningand histology.Serumlevels of osteocalcin(OCN)and cross-linked N-telopeptide of type I collagen (NTx) were measured by enzyme-linked immunosorbent assay. The decreases in new bone formation (p<0.05), calvaria thickness (p<0.05), bone volume (p<0.05), midline suture area (p<0.05), and OCN concentration (p<0.05) found in diabetic mice were normalized with naringin treatment. Diabetic state promoted particle-induced osteolysis. Naringin, an osteoanabolic agent, improved bone indices apparently by stimulating bone formation. Therefore, naringin may be beneficial in preventing and treating debris-mediated periprosthetic osteolysis after total joint replacement, especially in diabetics. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.


Chang C.,Kunhua Hospital of Kunming Medical College | Wang P.,Kunhua Hospital of Kunming Medical College | Yang H.,Kunhua Hospital of Kunming Medical College | Li L.,Kunhua Hospital of Kunming Medical College | Zhang L.-B.,Kunhua Hospital of Kunming Medical College
Journal of Sichuan University (Medical Science Edition) | Year: 2011

Objective: To investigate the expression of lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), the homeobox gene (Prox-1), in patients with non-small cell lung cancer (NSCLC), the relationship with microlymphatic vessel density, lymph node metastasis and their clinic pathological value. Methods Forty specimens of the NSCLC as experimental group and eleven pulmonary benign diseases as control group were studied. The expressions of LYVE-1, Prox-1 and CD31 protein in specimens of NSCLC and normal pulmonary tissue were studied with immunohistochemical (IHC) technique. Microlymphatic vessel density (MLVD) and microvessel density (MVD) were counted. Meanwhile, all specimens were also examined by conventional pathological method. Clinicopathological data of each patient were recorded and analyzed. Results 1 Among 40 cases of the center of NSCLC cancerous tissues, the MLVDs marked by LYVE-1 and Prox-1 were 4. 22 ± 1. 25 and 1. 99 ± 1. 49 respectively, which were significantly lower than those of the pulmonary benign diseases tissues (P = 0. 000). 2 The MLVDs marked by LYVE-1 and Prox-1 in NSCLC cancerous invasive edge were 10. 89±2. 06 and 6. 63±1. 99 respectively, which were significantly higher than those in the center of cancerous tissues and those of the pulmonary benign diseases tissues (P=0. 000). 3 The MLVDs marked by LYVE-M and Prox-1 in cancerous invasive edge were not correlated with age. gender, site and dimension of lesion, types of histological and degree of differentiation, but correlated significantly with lymph node metastasis (P=0. 000) and PTNM stage (P = 0. 000). Meanwhile, along with lymph node metastasis and increasing of PTNM stage, the expressions of LYVE-1 and Prox-1 protein and MLVDs have significantly increased, but the microvessel density marked by CD31 in cancerous invasive edge was not correlated significantly with lymph node metastasis (P = 0. 450) and PTNM stage (P=0. 377). 4 Significant correlation between LYVE-1 and Prox-1 (r=0. 529, P=0. 000) expression was observed in NSCLC, moreover, no correlations between LYVE-1 and CD31, Prox-1 and CD31 (r=0. 034, P = O. 837; r= -0. 075, P = 0. 647) were observed. Conclusion The functional microlymphatic vessels correlated with lymphatic metastasis are mainly located in the cancerous invasive edge rather than the center of cancerous tissues. LYVE-1 and Prox-1 might be acted as molecular phenotypes of lymphangioghesis in NSCLC and as important markers for evaluating lymphatic metastasis and prognosis in patients with NSCLC.

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