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Zhou X.,Taizhou University | Zhou X.,Shanghai JiaoTong University | Zhang C.,Kunhua Hospital of Kunming Medical College | Wang X.,Shanghai JiaoTong University | And 3 more authors.
Journal of Surgical Research | Year: 2012

Background: Wear particle-induced osteolysis could lead to the aseptic loosening of implants. Studies have suggested that endotoxins, such as lipopolysaccharides (LPS), may be the primary causes of wear particle-mediated osteolysis, and that osteolysis may originate from subclinical levels of bacterial infection. However, effective therapies against wear particles and gram-negative bacterial or LPS-induced bone resorption are limited. Materials and Methods: In the current study, the effect of berberine on LPS- and polyethylene (PE) particle-induced osteolysis in vivo was investigated using a mouse calvarial model. Osteoclast number per bone perimeter and eroded surface per bone surface were measured. Results: Berberine (10 mg/kg), injected either simultaneously with LPS or 3 d after LPS (25 mg/kg) treatment, blocked LPS-induced osteoclast recruitment and bone resorption in the mouse calvarial model. A daily single-dose of berberine (10 mg/kg), injected either simultaneously with PE particles or 4 d after treatment with PE particles, blocked PE particle-induced osteoclast recruitment and bone resorption. Berberine treatment markedly decreased LPS and PE particle-induced osteoclast recruitment and bone resorption in the murine calvarial model. Conclusion: These results suggest that berberine may have therapeutic effect for osteolysis induced by wear particles and LPS in gram-negative bacteria. © 2012 Elsevier Inc. All rights reserved.


Zhou X.,Shanghai JiaoTong University | Zhou X.,Taizhou University | Zhang P.,Chinese University of Hong Kong | Zhang C.,Kunhua Hospital of Kunming Medical College | And 2 more authors.
Calcified Tissue International | Year: 2010

An experiment was designed to investigate whether systemic administration of tetracyclines (TCs) as bone fluorochrome labels could interfere with bone modeling in vivo and inhibit osteoclast formation and activity in vitro. Cell cultures of rat bone marrow macrophages revealed that TC and oxytetracycline inhibited osteoclastogenesis and bone resorption and stimulated apoptosis. Forty rats in five groups were treated with saline, calcein green, alizarin red S, TC, or oxytetracycline. Their tibias were used for histomorphometric analysis, including bone static, dynamic, and resorption parameters in the tibial proximal metaphysis. No significant differences in bone volume per tissue volume, trabecular number, trabecular thickness, trabecular separation, bone formation rate per bone surface, mineralizing surface, or mineral apposition rate were observed. TC or oxytetracycline decreased eroded surface, number of osteoclasts per bone perimeter, and osteoclast surface per bone surface by about 50%. The results demonstrated that TC and oxytetracycline inhibit rat osteoclast formation and activity in vitro, and histomorphometric parameters involved in bone turnover may be affected by the use of oxytetracycline and TC as fluorescent bone labels in vivo. © 2009 Springer Science+Business Media, LLC.


Zhou X.,Shanghai JiaoTong University | Zhou X.,Taizhou University | Zhang P.,Chinese University of Hong Kong | Zhang C.,Kunhua Hospital of Kunming Medical College | Zhu Z.,Shanghai JiaoTong University
Journal of Orthopaedic Research | Year: 2010

Diabetic patients have an increased risk of prosthesis failure requiring revision surgery. Furthermore, skeletal defects are observed in conjunction with type 1 diabetes. Using a titanium particle-induced calvarial osteolysis model in diabetic mice, we investigated the effect of diabetes on the osteolytic process and the role of naringin in its prevention. Three groups each of nondiabetic or diabetic mice were treated with vehicle only, with particles only, or with particles then naringin for 10 days. Alteration of bone indices near the midline suture were then analyzed by microcomputed tomography scanningand histology.Serumlevels of osteocalcin(OCN)and cross-linked N-telopeptide of type I collagen (NTx) were measured by enzyme-linked immunosorbent assay. The decreases in new bone formation (p<0.05), calvaria thickness (p<0.05), bone volume (p<0.05), midline suture area (p<0.05), and OCN concentration (p<0.05) found in diabetic mice were normalized with naringin treatment. Diabetic state promoted particle-induced osteolysis. Naringin, an osteoanabolic agent, improved bone indices apparently by stimulating bone formation. Therefore, naringin may be beneficial in preventing and treating debris-mediated periprosthetic osteolysis after total joint replacement, especially in diabetics. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.


Tang H.,Institute of Basic Medicine | Zhang C.,Kunhua Hospital of Kunming Medical College | Li L.,Kunhua Hospital of Kunming Medical College | Yan X.-M.,Institute of Basic Medicine | Guo Q.,Kunhua Hospital of Kunming Medical College
World Chinese Journal of Digestology | Year: 2010

AIM: To detect the expression of CXC chemokine receptor 4 (CXCR4) and CC chemokine receptor 7 (CCR7) in normal esophageal mucosa, Barrett' s esophagus (BE), esophageal adenocarcinoma (EADC), and esophageal squamous cell carcinoma (ESCC), and to investigate their clinical significance. METHODS: The expression of CXCR4 and CCR7 in 56 normal esophageal mucosal specimens, 80 BE specimens (including 22 cases of BE with multifocal atypical hyperplasia), 25 EADC specimens, and 48 ESCC specimens was examined by immunohistochemistry. The expression levels of CXCR4 and CCR7 were then quantified and analyzed statistically. RESULTS: The positive expression rates of CXCR4 and CCR7 in BE, EADC and ESCC were significantly higher than those in normal esophageal mucosa (CXCR4: 78.75%, 68.00%, 83.33% vs 39.29%; CCR7: 60.00%, 60.00%, 58.33% vs 30.36%; all P < 0.01). However, there were no significant differences in the positive expression rates of CXCR4 and CCR7 among BE, EADC and ESCC. The positive expression rates of CXCR4 were significantly higher than those of CCR7 in both BE and ESCC (P < 0.05 and 0.01). CXCR4 and CCR7 expression was not associated with gender, age, and lesion site in BE, EADC and ESCC, but correlated with tumor differentiation and lymph node metastasis in EADC (both P < 0.05) and TNM stage, lymph node metastasis, and differentiation in ESCC (all P < 0.05). A significant correlation was noted between CXCR4 and CCR7 expression in BE and EADC (r = 0.262, 0.490), but not in ESCC (r = 0.076). CONCLUSION: Combined detection of CXCR4 and CCR7 expression may contribute to more accurate diagnosis of BE, EADC and ESCC. High expression levels of CXCR4 and CCR7 can be used as important parameters for evaluating tumor invasion and metastasis in both EADC and ESCC.


Chen Z.-M.,Kunhua Hospital of Kunming Medical College | Lv L.,Kunhua Hospital of Kunming Medical College | Li W.-J.,Kunhua Hospital of Kunming Medical College | Zhang J.-C.,Kunhua Hospital of Kunming Medical College | Gong H.-F.,Kunhua Hospital of Kunming Medical College
Chinese Journal of Medical Imaging Technology | Year: 2011

Objective: To observe the value of dual-source CT (DSCT) angiography in the follow-up of patients after transjugular intrahepatic portosystemic shunt (TIPS). Methods: Totally 28 patients underwent TIPS for liver cirrhosis, portal hypertension with upper gastrointestinal hemorrhage or mass ascites. All patients underwent DSCT examination within 1 month or 3 months, and 6 months, 12 months, 18 months, and 24 months after TIPS. MIP and VR were performed to determine the position and patency of the shunt between the hepatic vein and portal vein. Results: Thrombosis was detected inside stent in 3 cases (10.71%), MIP showed low density lesions, contrast agent flowed from stenosis, VR displayed good position and angle of stent. After balloon expansion, patent shunt was obtained again in the above 3 patients. The stent was lengthy and angular in 1 patient (3.57%), who then underwent the secondary stenting. No thrombosis nor stenosis was found in 24 patients (85.71%), among them MIP showed the patent stent and contrast agent, VR displayed the satisfied position and angle of the stents. Conclusion: DSCT angiography is safe, non-invasive and accurate, able to display the parenchyma of liver, therefore can be regarded as the first choice for the follow-up of patients after TIPS.

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