Thapliyal S.,Hemwati Nandan Bahuguna Garhwal University |
Juyal V.,Kumoun University |
Bhandari A.,Jodhpur National University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014
The present study investigated the antioxidant and hepatoprotective activity of methanolic rhizome extract of Hedychium Spicatum (MEHS) in CCl4-induced hepatotoxicity model in rats. The hepatoprotective and in vivo antioxidant activity of methanolic rhizome extract of Hedychium Spicatum were evaluated against CCl4-induced hepatic damage in rats. The MEHS at dose of 100, 200 and 400 mg/kg were administered orally once daily for seven days. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues. Further histopathological examination of the liver sections was carried out to support the induction of hepatotoxicity and hepatoprotective efficacy. The extract revealed significant activities and substantially elevated serum enzymatic levels of AST, ALT, ALP and total bilirubin were found to be restored towards normalization significantly by the MEHS in a dose dependent manner with maximum hepatoprotection at 400 mg/kg dose level. The histopathological results also supported the biochemical evidences of hepatoprotection. Elevated level of superoxide dismutase (SOD) and decreased level of malondialdehyde (MDA) further strengthen the hepatoprotective observations. The results of the present study strongly revealed that MEHS have potent antioxidant activity and hepatoprotective activity against CCl4-induced hepatic damage in experimental animals.
Chhavi A.,Kumoun University |
Sarita Dr. S.,US Foods
Malaysian Journal of Nutrition | Year: 2012
Introduction: This study was undertaken with the objectives of formulating composite bread by utilising finger millet flour and foxtail millet flour and further to evaluate these breads for sensory, nutritional qualities, and glycemic response. Methods: Two genotypes of finger millet VL-146 and PRM-601 and one local cultivar of foxtail millet were studied. The finger millet flour (FMF) and foxtail millet flour (FTF) were individually blended in various proportions (30 to 60%) into refined wheat flour (RWF). These blends were then used in the preparation of composite breads. The refined wheat flour bread (RWF) served as the control. One bread from each millet flour blend was selected finally for further investigation on the basis of sensory scores. Results: As the 30% millet flour substitution was most preferred among the three millet samples, it was selected for further evaluation. Nutrient composition of the selected breads showed that composite bread formulated using FTF showed significantly higher crude protein, crude fat, total ash, phosphorus and insoluble dietary fibre. The composite bread formulated using FMF contained significantly higher calcium, soluble dietary fibre, tannin and phytic acid. However, the control (RWF) bread contained significantly higher carbohydrate, physiological energy and starch. The lowest value for glycemic index among the breads was observed for bread containing FMF from genotype VL- 146 (41.43), followed by bread containing FMF from the genotype PRM- 601 (43.10), bread containing FTF (49.53) and control bread (67.82). Conclusion: Millet flour incorporated breads had low glycemic indices and were acceptable and nutritious.
Sati J.,Kumoun University |
Pande V.,Kumoun University |
Sah S.,Kumoun University |
Barat A.,Kumoun University
Journal of Ecophysiology and Occupational Health | Year: 2013
Tor putitora is the most economically important for both food and game fish in the Trans Himalayan region. A number of 15 microsatellite loci from a mahseer (T. tambroides) and snow trout (Schizothorax richardonii) were used to study their utility for cross species amplification in T. putitora. Seven of them successfully optimized through PCR. Moderate levels of heterozygosity (0.931-0.000) with allele number ranged from 4 -9 and were observed for these seven loci. The present study confirmed the transferability of some heterologous microsatellite loci from one species to another. These seven set of markers would be useful to investigate a fine scale population genetic analysis in T. putitora. ©2013 The Academy of Environmental Biology,India.