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Kumamoto-shi, Japan

Pawat J.,Waseda University | Pawat J.,Lublin University of Technology | Stryczewska H.D.,Lublin University of Technology | Ebihara K.,Kumamoto Techno Research Park
Journal of Advanced Oxidation Technologies | Year: 2010

The sterilizing and remediation effects of the non-equilibrium atmospheric pressure plasmas are known for decades. Low temperature atmospheric pressure plasmas are considered as a promising alternative to conventional sterilizing methods, which have numerous drawbacks. Influence of various parameters such as discharge regimes, reactor geometries, gases, etc., on formation and effectiveness of plasma were investigated by many research groups. This paper presents the review of recently developed, environmentally safe technologies applied for the agriculture and soil remediation. The results of ozone soil sterilization and ozone influence on the DNA structure of Escherichia coli are described. The results of oxidants influence on humic acid are presented. © 2010 Science and Technology Network, Inc.

Iwashita H.,Kumamoto Techno Research Park | Shiraki N.,Kumamoto University | Sakano D.,Kumamoto University | Ikegami T.,Kumamoto Techno Research Park | And 3 more authors.
PLoS ONE | Year: 2013

To date, CXCR4 and E-cadherin double-positive cells detected by flow cytometry have been used to identify the differentiation of embryonic stem (ES) cells or induced pluripotent stem (iPS) cells into definitive endoderm (DE) lineages. Quantification of DE differentiation from ES/iPS cells by using flow cytometry is a multi-step procedure including dissociation of the cells, antibody reaction, and flow cytometry analysis. To establish a quick assay method for quantification of ES/iPS cell differentiation into the DE without dissociating the cells, we examined whether secreted Cerberus1 (Cer1) protein could be used as a marker. Cer1 is a secreted protein expressed first in the anterior visceral endoderm and then in the DE. The amount of Cer1 secreted correlated with the proportion of CXCR4+/E-Cadherin+ cells that differentiated from mouse ES cells. In addition, we found that human iPS cell-derived DE also expressed the secreted CER1 and that the expression level correlated with the proportion of SOX17+/FOXA2+ cells present. Taken together, these results show that Cer1 (or CER1) serves as a good marker for quantification of DE differentiation of mouse and human ES/iPS cells. © 2013 Iwashita et al.

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