Kumamoto Prefectural Agriculture Research Center

Koshi, Japan

Kumamoto Prefectural Agriculture Research Center

Koshi, Japan
SEARCH FILTERS
Time filter
Source Type

Kaneda M.,Tokyo University of Agriculture and Technology | Takahashi M.,Hokkaido University | Yamanaka K.-I.,Saga University | Saito K.,Kumamoto Prefectural Agriculture Research Center | And 4 more authors.
Journal of Reproduction and Development | Year: 2017

Although more than 100 imprinted genes have already been identified in the mouse and human genomes, little is known about genomic imprinting in cattle. For a better understanding of these genes in cattle, parthenogenetically activated bovine blastocysts were transferred to recipient cows to obtain parthenotes, and fibroblasts derived from a Day 40 (Day 0 being the day of parthenogenetic activation) parthenogenetic embryo (BpEFs) were successfully obtained. Bovine embryonic fibroblasts (BEFs) were also isolated from a normal fertilized embryo obtained from an artificially inseminated cow. The expression of imprinted genes was analyzed by RT-PCR. Paternally expressed genes (PEGs) in mouse (viz., IGF2, PEG3, ZAC1, NDN, DLK1, SGCE, and PEG10) were expressed in BEFs, but not in BpEFs, suggesting that these genes are also imprinted in cattle. However, other PEGs in mouse (viz., IMPACT, MAGEL2, SNRPN, and PEG1/MEST) were expressed in both BEFs and BpEFs. These genes may not be imprinted in BEFs. The expression of seven maternally expressed genes in mouse was also analyzed, and only CDKN1C was not expressed in BpEFs. The DNA methylation patterns of repetitive elements (Satellite I, Satellite II, alpha-satellite, and Art2) were not different between the BEFs and BpEFs; however, the differentially methylated region (DMR) of paternally methylated H19 was hypomethylated, whereas those of maternally methylated PEG3 and PEG10 were hypermethylated in BpEFs, as expected. The methylation of the SNRPN DMR was not different between the BEFs and BpEFs, in accordance with the SNRPN expression levels in both cell types. The XIST gene, which is essential for X chromosome inactivation in females, was expressed in BpEFs, whereas its DMR was half-methylated, suggesting that X chromosome inactivation is normal in these cells. Microarray analysis was also applied to identify novel PEGs that should be expressed only in BEFs but not in BpEFs. More than 300 PEG candidate genes, including IGF2, PEG3, and PEG10, were obtained. These results illustrate the epigenetic characteristic of bovine parthenogenetic embryos and contribute to the identification of novel imprinted genes in cattle. © 2017 by the Society for Reproduction and Development.


Noda T.,Kumamoto Prefectural Agriculture Research Center | Iimure K.,Kumamoto Prefectural Agriculture Research Center | Okamoto S.,Takii and Company | Saito A.,Kumamoto Prefectural Agriculture Research Center
Bioscience, Biotechnology and Biochemistry | Year: 2017

Browning of plant tissue is generally considered attributable to enzymatic oxidation by polyphenol oxidase (PPO). Electrophoresis followed by activity staining has been used as an effective procedure to visually detect and isolate isozymes; however, it has not been applied for examination of various PPO isozymes in lettuce. Our study demonstrated that different lettuce PPO isozymes could be detected at different pH in active staining, and multiple isozymes were detected only under alkaline conditions. As a result, we concluded that activity staining with approximately pH 8 enabled to detect various PPO isozymes in lettuce. By expression analysis of the PPO isozymes after wounding, PPO isozymes that correlated with time-course of tissue browning were detected. The wound-induced PPO may play a key role in enzymatic browning. © 2017 Japan Society for Bioscience, Biotechnology, and Agrochemistry.


Noda T.,Kumamoto Prefectural Agriculture Research Center | Meguri T.,Daito Fertilizer Co. | Iimure K.,Kumamoto Prefectural Agriculture Research Center | Ono M.,Tokai University | Araki T.,Tokai University
Bioscience, Biotechnology and Biochemistry | Year: 2011

D-ery-Ci4-Sphingosin (Ci4-Sph) was isolated as the germination accelerating factor in Nomuraea rileyi in our previous study. This activity was expected to support fungal infection by reduction of the infection time between conidial adhesion and invasion into the insect. In this study, we estimated the effect of CM-Sph with regard to the infection time. Conidia activated by Ci4-Sph shortened the time to about half, indicating the potential of Ci4-Sph as an adjuvant for fungal pesticide of N. rileyi.


Noda T.,Kumamoto Prefectural Agriculture Research Center | Noda T.,Tokai University | Satoh T.,Kumamoto Industrial Research Institute | Iimure K.,Kumamoto Prefectural Agriculture Research Center | And 2 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2011

D-erythro-C14-Sphingosine (C14-Sph) accelerated the germination of Nomuraea rileyi in a solution containing peptone, but activity declined to a large degree in water. This suggests the presence of a co-factor in C14-Sphtriggered germination. Since the main role of peptone is to supply nitrogen constituents, we examined the effects of various nitrogen constituents. It was found that Ala and His were highly effective for C 14-Sph-triggered germination.


Noda T.,Kumamoto Prefectural Agriculture Research Center | Noda T.,Tokai University | Ono M.,Tokai University | Iimure K.,Kumamoto Prefectural Agriculture Research Center | Araki T.,Tokai University
Bioscience, Biotechnology and Biochemistry | Year: 2010

The conidium of the entomopathogenic fungus Nomuraea rileyi has been found to germinate rapidly in the presence of host insect-derived extracts. Thus the extract appears to contain an important factor involved in host recognition by N. rileyi. However, the substance responsible for such unique germination behavior has yet to be identified. Hence we attempted to purify this substance. One thousand g of dried silkworm pupae was subjected to methanol extraction, followed by methanolysis, two different solvent partitions, and three different column chromatographies. A total of 12.4mg of substance was obtained in the active fraction. The substance obtained exhibited an activity more than 46,000 times higher than that of the methanol extract. The substance was detected as a single peak on Sephadex LH20 column chromatography and as a single band on high-performance thin-layer chromatography. These data indicate that the concentrated fraction contained a high-purity substance.


Noda T.,Kumamoto Prefectural Agriculture Research Center | Noda T.,Tokai University | Ono M.,Tokai University | Iimure K.,Kumamoto Prefectural Agriculture Research Center | Araki T.,Tokai University
Bioscience, Biotechnology and Biochemistry | Year: 2010

The conidium of the entomopathogenic fungus, Nomuraea rileyi, has been found to germinate rapidly in the presence of a host insect-derived extract. This extract therefore appears to contain an important factor involved in host recognition by N. rileyi, although the substance (germination-accelerating factor, GAF) responsible for such unique germination behavior has yet to be identified. Our previous study was extended to the isolation of GAF from pupae of the silkworm, a host insect of N. rileyi. This present work subjects GAF to a structural analysis. The chemical structure of GAF is characterized as 2S-amino-tetradeca-4-ene-l, 3R-diol (D-erythro-Cl4-sphingosine) based on spectroscopic data. An examination of the structure-activity relationship shows that the activity of D-erythro-C14-sphingosine was superior to that of sphingosines with shorter and longer carbon chains. It is suggested that the molecular species with a 14-carbon chain of a sphingosine is important for host recognition.


PubMed | Kumamoto Prefectural Agriculture Research Center
Type: Journal Article | Journal: Bioscience, biotechnology, and biochemistry | Year: 2011

D-erythro-C(14)-Sphingosin (C(14)-Sph) was isolated as the germination accelerating factor in Nomuraea rileyi in our previous study. This activity was expected to support fungal infection by reduction of the infection time between conidial adhesion and invasion into the insect. In this study, we estimated the effect of C(14)-Sph with regard to the infection time. Conidia activated by C(14)-Sph shortened the time to about half, indicating the potential of C(14)-Sph as an adjuvant for fungal pesticide of N. rileyi.


PubMed | Kumamoto Prefectural Agriculture Research Center
Type: Journal Article | Journal: Bioscience, biotechnology, and biochemistry | Year: 2010

The conidium of the entomopathogenic fungus, Nomuraea rileyi, has been found to germinate rapidly in the presence of a host insect-derived extract. This extract therefore appears to contain an important factor involved in host recognition by N. rileyi, although the substance (germination-accelerating factor, GAF) responsible for such unique germination behavior has yet to be identified. Our previous study was extended to the isolation of GAF from pupae of the silkworm, a host insect of N. rileyi. This present work subjects GAF to a structural analysis. The chemical structure of GAF is characterized as 2S-amino-tetradeca-4-ene-1,3R-diol (D-erythro-C(14)-sphingosine) based on spectroscopic data. An examination of the structure-activity relationship shows that the activity of D-erythro-C(14)-sphingosine was superior to that of sphingosines with shorter and longer carbon chains. It is suggested that the molecular species with a 14-carbon chain of a sphingosine is important for host recognition.

Loading Kumamoto Prefectural Agriculture Research Center collaborators
Loading Kumamoto Prefectural Agriculture Research Center collaborators