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Norlelawati A.T.,International Islamic University Malaysia | Mohd Danial G.,Kulliyyah of Science | Nora H.,Hospital Tengku Ampuan Afzan Hospital | Nadia O.,International Islamic University Malaysia | And 3 more authors.
Malaysian Journal of Pathology | Year: 2016

Background: Synovial sarcoma (SS) is a rare cancer and accounts for 5-10% of adult soft tissue sarcomas. Making an accurate diagnosis is difficult due to the overlapping histological features of SS with other types of sarcomas and the non-specific immunohistochemistry profile findings. Molecular testing is thus considered necessary to confirm the diagnosis since more than 90% of SS cases carry the transcript of t(X;18)(p11.2;q11.2). The purpose of this study is to diagnose SS at molecular level by testing for t(X;18) fusion-transcript expression through One-step reverse transcriptase real-time Polymerase Chain Reaction (PCR). Method: Formalin-fixed paraffin-embedded tissue blocks of 23 cases of soft tissue sarcomas, which included 5 and 8 cases reported as SS as the primary diagnosis and differential diagnosis respectively, were retrieved from the Department of Pathology, Tengku Ampuan Afzan Hospital, Kuantan, Pahang. RNA was purified from the tissue block sections and then subjected to One-step reverse transcriptase real-time PCR using sequence specific hydrolysis probes for simultaneous detection of either SYT-SSX1 or SYT-SSX2 fusion transcript. Results: Of the 23 cases, 4 cases were found to be positive for SYT-SSX fusion transcript in which 2 were diagnosed as SS whereas in the 2 other cases, SS was the differential diagnosis. Three cases were excluded due to failure of both amplification assays SYT-SSX and control β-2-microglobulin. The remaining 16 cases were negative for the fusion transcript. Conclusion: This study has shown that the application of One-Step reverse transcriptase real time PCR for the detection SYT-SSX transcript is feasible as an aid in confirming the diagnosis of synovial sarcoma. © 2006, Malaysian Society of Pathologists. All rights reserved.


Norlelawati A.T.,International Islamic University Malaysia | Rusmawati I.,Hospital Tengku Ampuan Afzan Hospital | Nur Nadia O.,International Islamic University Malaysia | Naznin M.,International Islamic University Malaysia | And 2 more authors.
Medical Journal of Malaysia | Year: 2014

Objective: Inherited anti-thrombin deficiency is an autosomal dominant disorder which is associated with increased risk for venous thromboembolism (VTE). This condition is very rare in Malaysia and there has been no documented report. Thus, the aim of the present study is to investigate the type of an inherited anti-thrombin deficiency mutation in a 25-year-old Malay woman who presented with deep vein thrombosis in her first pregnancy. Methods: DNA was extracted from the patient's blood sample and buccal mucosal swabs from family members. Polymerase chain reaction(PCR) assays were designed to cover all seven exons of the serpin peptidase inhibitor, clade C (antithrombin), member 1 (SERPINC1) gene; and the products were subjected to DNA sequencing. Sequences were referred to NCBI Reference Sequence: NG_012462.1. Results: A heterozygous substitution mutation at nucleotide position 13267 (CCT->ACT) was identified in the patient and two other family members, giving a possible change of codon 439 (Pro→Thr) also known as anti-thrombin Budapest 5. The genotype was absent in 90 healthy controls. Conclusion: The study revealed a heterozygous antithrombin Budapest 5 mutation in SERPINC 1 giving rise to a possible anti-thrombin deficiency in a Malay-Malaysian family.


Abdullaev F.K.,Kulliyyah of Science | Brazhnyi V.A.,University of Porto | Salerno M.,University of Salerno
Nonlinear Photonics, NP 2014 | Year: 2014

The resonant scattering of gap solitons of the periodic NLS equation with a PT-symmetric defect is investigated. For suitable parameters of the defect potential the resonant transmission of soliton through the defect becomes possible. © 2014 OSA.


Messikh A.,Kulliyah of Information and Communication Technology | Umarov B.,Kulliyyah of Science
Journal of Physics: Conference Series | Year: 2013

We use homoclinic orbits to find solutions of a dynamical system of the dipolar Bose Einstein Condensate (BEC) in a deep optical lattice. The equation of motion is transformed to a two-dimensional map and its homoclinic orbits are computed numerically. Each homoclinic orbit leads to a different solution. These different solutions lead to different types of solitons. We also analyse the stability of the solutions. © Published under licence by IOP Publishing Ltd.


Wu B.,Chinese Academy of Fishery Sciences | Xia S.,Tianjin Fisheries Research Institute | Rahman M.M.,International Islamic University Malaysia | Rajkumar M.,Kulliyyah of Science | And 3 more authors.
Aquaculture | Year: 2015

A 60-day experiment was conducted to develop a practical diet for sea cucumber (Apostichopus japonicus) substituting seaweed with corn leaf. Five experimental diets were constructed with each containing a 70% sea mud. The other 30% is composed of (A) 30% Sargassum thunbergii (seaweed) only, (B) 22.5% seaweed and 7.5% corn (Zea mays) leaves, (C) 15% seaweed and 15% corn leaves, (D) 7.5% seaweed and 22.5% corn leaves and (E) 30% corn leaves only. The experiment was conducted in 15 plastic aquaria containing seawater, each of which stocked with 20 sea cucumbers. Three replicate groups of sea cucumber were fed one of the five experimental diets. The results revealed that the apparent digestibility coefficients for dry matter (ADMD) and crude protein (ACPD) of the test diets decreased with increasing corn leaf content in diets. Corn leaf content in the sea cucumber diet did not affect the body composition of sea cucumber. 22.5% seaweed can be replaced with powdered corn leaf in the sea cucumber diet without any negative effects on weight gain and specific growth rate (SGR) of sea cucumber. Diet containing higher than 15% corn leaf significantly increased the feed conversion ratio (FCR) of sea cucumber. However, the growth performance and FCR of sea cucumber fed the mixed diet of 15% corn leaf plus 15% seaweed were better than the growth performance and FCR of sea cucumber fed other experimental diets. The regression analysis of dietary corn leaf inclusion level versus SGR revealed that the optimal corn leaf inclusion level was 11.2% for the best growth rate of sea cucumber. Therefore, under the present experimental design and condition, a diet with 70% sea mud plus 18.8% seaweed plus 11.2% corn leaf can be recommended for the best growth performance of sea cucumber. The findings of this study will encourage feed manufacturers and sea cucumber culturists to utilize corn leaf in producing low-cost aqua-feed. © 2015 Elsevier B.V.

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