Sukri N.M.,Kulliyyah of Allied Health science |
Radzi M.A.A.,Kulliyyah of Allied Health science |
Rahman R.A.,Kulliyyah of Allied Health science |
Zulkifly A.H.,Traumatology and Rehabilitation |
And 2 more authors.
Jurnal Teknologi | Year: 2015
Sox9 plays an important role as transcription factor for chondrogenesis; the formation of cartilage. This study aimed to identify the potential of the transiently overexpressed Sox9 gene in human chondrocytes differentiation and tissue engineered cartilage (TEC) formation in vitro. Articular cartilage samples were obtained from osteoarthritic patients who underwent joint replacement surgery. The isolated chondrocytes were cultured and transfected with pcDNA3-Sox9 using lipofection technique. The TEC constructs were formed by incorporating the transfected and the nontransfected cells onto poly(lactic-co-glycolic acid) (PLGA) scaffold with or without fibrin. This approach allows a comparison between four groups i.e. (1) transfected chondrocytes seeded on PLGA/fibrin PFTC], (2) nontransfected chondrocytes on PLGA/fibrin PFC], (3) transfected chondrocytes on PLGA PTC. and (4) non-transfected chondrocytes on PLGA PC]. All TEC constructs were cultured and evaluated at each time point of 1, 2 and 3 weeks in vitro. All TEC constructs were analysed for gross observation, histology, immunohistochemistry, cell proliferation activity, gene expression and sulphated glycosaminoglycan (sGAG) production assay. After 3 weeks, all PFTC and PFC showed higher cell viability, higher sGAG content, better histological features and distribution of extracellular matrix in concert with positive glycosaminoglycan (GAG) accumulation when compared to the PTC and PC. However, at week 3, the PFC and PC exhibited significantly higher sGAG production than PFTC and PTC. Chondrogenic properties of the constructs were evidenced by the expression of cartilage-specific markers; collagen II, collagen XI and aggrecan core protein. In this study, due to the nature of a new cartilage formation, the co-expression of collagen I in all constructs can be an indication of early cartilage development. Based on the outcomes, it is hoped that this study will provide a good ground for future tissue engineering application. © 2015 Penerbit UTM Press. All rights reserved.
PubMed | University of Western Ontario, University of Kuala Lumpur, Darul Iman University, Malaysia, Eastern Medical College and 4 more.
Type: | Journal: Psychology research and behavior management | Year: 2016
The use of Internet has now become indispensable, and the technology has revolutionized the medical education and practice worldwide. Currently, medical students and professionals have an enormous opportunity to keep them always updated with the exponential growth of knowledge because of potential progression of Internet throughout the world that enables them to become a lifelong learner. Internet addiction is a widespread phenomenon among students and academicians at universities in Malaysia. Students use the Internet for recreational purpose and personal and professional development. The Internet has become an integral part of day-to-day life of the university students, including medical students. The aim of the present study was to examine the Internet use and addiction among students of Universiti Sultan Zainal Abidin, Malaysia.This was a cross-sectional study in which a questionnaire, Internet Addiction Diagnostic Questionnaire, developed by the Center for Internet Addiction, USA, was used. One hundred forty-nine medical students of Universiti Sultan Zainal Abidin participated in this study. Data were analyzed using Statistical Package for the Social Sciences software.The mean scores were 44.914.05 and 41.413.05 for male and female participants, respectively, which indicated that both the genders were suffering from mild Internet addiction.This study shows almost similar level of Internet usage among medical students irrespective of their socioeconomic background, with no statistically significant (
Chahal S.,Universiti Malaysia Pahang |
Hussain F.S.J.,Universiti Malaysia Pahang |
Kumar A.,Czech Technical University |
Rasad M.S.B.A.,Kulliyyah of Allied Health science |
Yusoff M.M.,Universiti Malaysia Pahang
Chemical Engineering Science | Year: 2016
Development of novel scaffold materials that mimic the extracellular matrix, architecturally and functionally, is becoming highly important to meet the demands of the advances in bone tissue engineering. This paper reports, the fabrication of natural polymer cellulose derived hydroxyethyl cellulose (HEC) based nanostructured scaffolds with uniform fiber morphology through electrospinning. Poly (vinyl alcohol) (PVA) was used as an ionic solvent for supporting the electrospinning of HEC. Scanning electron microscopy and ImageJ analysis revealed the formation of non-woven nanofibers with well-defined porous architecture. The interactions between HEC and PVA in the electrospun nanofibers were studied by differential scanning calorimetry, X-ray diffraction, dynamic mechanical analysis thermo-gravimetric analysis; Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and tensile test. The mechanical properties of scaffolds were significantly altered with different ratios of HEC/PVA. Further, the biocompatibility of HEC/PVA scaffolds was evaluated using human osteosarcoma cells. The SEM images revealed favorable cells attachment and spreading on the nanofibrous scaffolds and MTS assay showed increased cell proliferation after different time periods. Thus, these results indicate that HEC based nanofibrous scaffolds will be a promising candidate for bone tissue engineering. © 2016 Elsevier Ltd.
Chahal S.,Universiti Malaysia Pahang |
Jahir Hussain F.S.,Universiti Malaysia Pahang |
Kumar A.,Czech Technical University |
Yusoff M.M.,Universiti Malaysia Pahang |
Bahari Abdull Rasad M.S.,Kulliyyah of Allied Health science
RSC Advances | Year: 2015
The aim of this study is to develop a facile and efficient scaffold from electrospun hydroxyethyl cellulose (HEC) functionalized with bone-like calcium phosphate (CaP). The HEC/PVA nanofibers were fabricated by electrospinning and mineralized by incubating in 10× simulated body fluid (SBF) for different period of times. After 24 h of incubation, the nanofibers were uniformly coated by a thin layer of mineral deposit. SEM, FTIR, and FESEM-EDS analyses confirm the deposition of CaP on the nanofibers. The nanostructured biomaterial maintained its fibrous and porous structure after mineralization. The XRD results suggest that the deposited mineral phase is a mixture of calcium phosphate hydrate and apatite. The mechanical properties of CaP coated scaffolds show similar tensile strength and elastic modulus with that of trabecular and proximal femoral bones. The cytocompatibility of the CaP coated HEC/PVA scaffolds were evaluated using human osteosarcoma cells. The CaP coated HEC/PVA scaffolds support cellular attachment and proliferation of osteosarcoma cells and will be a promising candidate for bone tissue engineering. © The Royal Society of Chemistry 2015.
Murad M.A.M.R.,Kulliyyah of Allied Health science |
Rahman N.A.A.,Kulliyyah of Allied Health science |
Rahman N.I.A.,Darul Iman University, Malaysia |
Haque M.,National Defence University of Malaysia
Journal of Applied Pharmaceutical Science | Year: 2016
Physical inactivity is becoming a global epidemic and lead to many diseases. This cross-sectional study tried to investigate knowledge, attitude and practice (KAP) regarding exercise among married and single person attending gymnasium and recreational parks. Furthermore, this study tried to look for factors associated with regularity of exercise and to find association between knowledge and attitude on practice of exercise. A total of 103 participants aged above 18 years old were purposely selected to answer an interview-guided questionnaire. Likert scale was used for participants to answer the questionnaire on KAP and the total score for each knowledge, attitude and practice was computed to find the associations using SPSS. The mean age of the respondent was 25.34 years (SD=5.699). Knowledge was not significantly associated with practice (r=0.071, p=0.475). There was also no significant correlation between practice and attitude score (r=0.006, p=0.510). Therefore, there is no association between knowledge and attitude on practice of exercise. Besides that, endurance exercise like jogging, cycling and walking was a respondent's choice as their exercise routine. Other than that, there are significant associations between factors of gender (p=0.014), marital status (p<0.001) and age (p=0.005) with regularity of exercise. This study found that single person is more regularly doing exercise than married people. Regularity of exercise could be influenced by motivation. Therefore, relevant policies and campaigns might be able to change and boost people towards practicing exercise regularly in Malaysia. © 2016 Mohamed Afif Asyraf bin Mohamed Roshdin Murad et al.
Roslen N.A.,Kulliyyah of Allied Health science |
Alewi N.A.M.,Kulliyyah of Allied Health science |
Ahamada H.,Kulliyyah of Allied Health science |
Rasad M.S.B.A.,Kulliyyah of Allied Health science
Asian Pacific Journal of Tropical Biomedicine | Year: 2014
Objective: To screen the cytotoxic activity of Melastoma malabathricum (M. malabathricum) against human breast cancer cell line (MCF-7) in vitro. Methods: A three steps extraction protocol using n-hexane, chloroform and methanol as the solvents systems was carried out on leaves, stems and flowers of M. malabathricum. Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations (100 μg/mL, 50 μg/mL, 25 μg/mL, 12.5 μg/mL and 6.25 μg/mL). The evaluation of cell growth was determined using methylene blue assay. Results: Methanol extract from the leaves showed significant anticancer activity against MCF-7 cell lines with the IC50 value of 7.14 μg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line with the IC50 value of 33.63 μg/μL and 45.76 μg/mL respectively after 72 h of treatment. Conclusions: The extracts from leaves and flowers of M. malabathricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50 at 7.14 μg/mL while the extracts from stems showed less growth inhibition activity. © 2014 by the Asian Pacific Journal of Tropical Biomedicine.
PubMed | Kulliyyah of Allied Health science
Type: Journal Article | Journal: Asian Pacific journal of tropical biomedicine | Year: 2014
To screen the cytotoxic activity of Melastoma malabathricum (M. malabathricum) against human breast cancer cell line (MCF-7) in vitro.A three steps extraction protocol using n-hexane, chloroform and methanol as the solvents systems was carried out on leaves, stems and flowers of M. malabathricum. Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations (100 g/mL, 50 g/mL, 25 g/mL, 12.5 g/mL and 6.25 g/mL). The evaluation of cell growth was determined using methylene blue assay.Methanol extract from the leaves showed significant anticancer activity against MCF-7 cell lines with the IC50 value of 7.14 g/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line with the IC50 value of 33.63 g/mL and 45.76 g/mL respectively after 72 h of treatment.The extracts from leaves and flowers of M. malabathricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50 at 7.14 g/mL while the extracts from stems showed less growth inhibition activity.