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Kononikhin A.S.,Moscow Institute of Physics and Technology | Chagovets V.V.,Kulakov Research Center for Obstetrics | Starodubtseva N.L.,Moscow Institute of Physics and Technology | Ryndin A.Y.,Kulakov Research Center for Obstetrics | And 7 more authors.
Molecular Biology | Year: 2016

Here, the possibility of proteomic and metabolomic analysis of the composition of exhaled breath condensate of neonates with respiratory support. The developed method allows non-invasive collecting sufficient amount of the material for identification of disease-specific biomarkers. Samples were collected by using a condensing device that was incorporated into the ventilation system. The collected condensate was analyzed by liquid chromatography coupled with high resolution mass spectrometry and tandem mass spectrometry. The isolated substances were identified with a use of databases for proteins and metabolites. As a result, a number of compounds that compose the exhaled breath condensate was determined and can be considered as possible biomarkers of newborn diseases or stage of development. © 2016, Pleiades Publishing, Inc.


Savilova A.M.,Kulakov Research Center for Obstetrics | Chulkina M.M.,Kulakov Research Center for Obstetrics | Speranskaya A.S.,Moscow State University | Trofimov D.Y.,Kulakov Research Center for Obstetrics
Russian Journal of Genetics | Year: 2014

We propose a method of quantitative functional activity assessment in cells isolated via sorting on a flow cytometer. We show that cell populations vary in the mRNA expression of cytokine genes immediately after isolation and sorting, while the maintenance of homogenous populations in culture without stimulation results in an increase in these gene mRNA expression. Using the original system, it is now possible to detect mRNA cytokine genes with high sensitivity, starting from 90 cells per specimen. This approach permits genetic and immunogenetic analysis of gene expression with the goal of determining their functions in the in vitro studies. © 2014 Pleiades Publishing, Inc.


Spiridonov I.N.,Moscow State Technical University | Nekhina A.A.,Moscow State Technical University | Uvarova E.V.,Kulakov Research Center for Obstetrics | Gautier M.S.,Kulakov Research Center for Obstetrics | Degtiarev D.N.,Kulakov Research Center for Obstetrics
Biomedical Engineering | Year: 2014

Characteristics of dermatoglyphic parameters of women with abnormal development of sex organs compared with the dermatoglyphic characteristics of women in a control group were investigated. An inkless method was used for recording fingerprints of the test and control groups, and values of complex dermatoglyphic parameters were defined. This article presents the results of a study that aims to find statistically significant dermatoglyphic parameters to determine presence of congenital abnormalities of sex organs in women. © 2014, Springer Science+Business Media New York.


PubMed | Moscow Institute of Physics and Technology and Kulakov Research Center for Obstetrics
Type: Journal Article | Journal: Molekuliarnaia biologiia | Year: 2016

Here, the possibility of proteomic and metabolomic analysis of the composition of exhaled breath condensate of neonates with respiratory support. The developed method allows non-invasive collecting sufficient amount of the material for identification of disease-specific biomarkers. Samples were collected by using a condensing device that was incorporated into the ventilation system. The collected condensate was analyzed by liquid chromatography coupled with high resolution mass spectrometry and tandem mass spectrometry. The isolated substances were identified with a use of databases for proteins and metabolites. As a result, a number of compounds that compose the exhaled breath condensate was determined and can be considered as possible biomarkers of newborn diseases or stage of development.

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