Honolulu, HI, United States

Kuehnle AgroSystems Corporation

www.kashawaii.com
Honolulu, HI, United States

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Patent
Kuehnle AgroSystems Corporation | Date: 2011-03-31

The present invention is directed to Gene Positioning technology for biosynthesis of one or more products of interest via plastid transformation of plants or algae, such as for example tobacco, Lemna, Rhodomonas and Cryptomonas, with pseudogene vectors containing polynucleotides encoding one or more products of interest. The present invention is also directed to transgenic plants or algae, containing pseudogene vectors integrated into a desired locus in the plastid genome, allowing simultaneous expression of multiple transgenes.


Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.2), (f) mevalonate diphosphate decarboxylase (EC 4.1.1.33), (g) isopentenyl diphosphate (IPP) isomerase (EC 5.3.3.2), and (h) phytoene synthase (EC 2.5.1.32).


Grant
Agency: Department of Agriculture | Branch: | Program: SBIR | Phase: Phase II | Award Amount: 349.98K | Year: 2009

Microalgae have long been employed in basic research of plant genetics and physiology. Thus, continued research and development are essential to attain commercial viability of microalgae as a renewable source of industrial product feedstocks. This project addresses genetic solutions for algae as feedstock biofactories.


Patent
Kuehnle AgroSystems Corporation | Date: 2015-08-31

The present invention relates to improved wastewater treatment systems and methods. In one embodiment, the present invention utilizes microbial consortia and the final effluent of the treated wastewater is remarkably clean, and has low levels of hazardous chemicals, unwanted nutrient values and/or total solids. Advantageously, the wastewater treatment system and method of the present invention reduces the use of chemical compounds in the treatment process. Further, valuable biomasses can be produced from the wastewater using the treatment process of the present invention.


Patent
Kuehnle AgroSystems Corporation | Date: 2014-03-13

The present invention relates to improved wastewater treatment systems and methods. In one embodiment, the present invention utilizes microbial consortia and the final effluent of the treated wastewater is remarkably clean, and has low levels of hazardous chemicals, unwanted nutrient values and/or total solids. Advantageously, the wastewater treatment system and method of the present invention reduces the use of chemical compounds in the treatment process. Further, valuable biomasses can be produced from the wastewater using the treatment process of the present invention.


The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.


Grant
Agency: Department of Defense | Branch: Navy | Program: STTR | Phase: Phase II | Award Amount: 493.00K | Year: 2010

The challenge has been set to meet Navy’s renewable fuel standard using advanced biofuels. Microalgae, along with other future fuel crops, must be aggressively developed now, not later. Recent dramatic demonstrations with algae based biodiesel and biojet show that it is no longer a question of algae being suitable as a natural source of oils that can be refined to produce biofuels. Rather, it is a question of being sustainable with long-term economic viability as algae farming becomes established at large scales. As an elite algae seedstock breeder/manufacturer, Kuehnle AgroSystems’s business is focused upon providing the biomass farmer with high performing, industrially relevant algae species to produce feedstock that meets price points for crude algae oil. In Phase I this project successfully completed a feasibility analysis to produce cost-effective biofuel from Kuehnle AgroSystems algae feedstock using a uniquely scaleable continuous supercritical methanol fuel conversion. In Phase II this project will expand fuel conversion capacity onto an industrial site next to a jetfuel refinery in Hawaii to experimentally demonstrate end-to-end process concepts to produce biofuels and co-products for Navy testing.


Grant
Agency: Department of Agriculture | Branch: | Program: SBIR | Phase: Phase II | Award Amount: 400.00K | Year: 2010

The scope of this SBIR project is to develop the Kuehnle AgroSystem's technical innovation of live algae preservation to provide room temperature storage options for microalgae that are preferred for specific aquaculture and biofuels applications. A major bottleneck in developing algae biomass for the aquaculture and cleantech industry is absence of quality bulk live algae product. Live algae concentrates are highly perishable and simply unavailable in the trade. High-performance algae are stabilized in a matrix at ambient temperatures as a viable cell concentrate for inventory storage and global shipping purposes. This novel solution permits a reliable route to seeding of aquaria, hatcheries, and algae biomass farms. This Phase II research project seeks to expand the number of algae species for preservation, scale-up to commercial volumes, define metrics for quality assurance for seedstock, and apply the technologies in aquaculture to build a strong business foundation with cross-over to biofuels. This project will satisfy the USDA's Strategic Goals to enhance rural and farm economics, and to protect and enhance the nation's food supply, natural resource base and environment.


Patent
Kuehnle AgroSystems Corporation | Date: 2014-03-13

The present invention relates to improved wastewater treatment systems and methods. In one embodiment, the present invention utilizes biologics and the final effluent of the treated wastewater is remarkably clean, and has low levels of hazardous chemicals, unwanted nutrient values and/or total solids. Advantageously, the wastewater treatment system and method of the present invention reduces the use of chemical compounds in the treatment process. Further, valuable biomasses can be produced or metals recovered from the wastewater using the treatment process of the present invention.


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