KTHM College

Nashik, India

KTHM College

Nashik, India

Time filter

Source Type

Hanamant P.S.,Kthm College
Journal of Pure and Applied Microbiology | Year: 2010

Psychrotiophic bacteria are capable of surviving in a cold environment (4°-7°C) regardless of their optimum growth temperature. They can be found in soil, deep sea water and in foods. The psychrotrophic bacteria isolated from dairy products can grow at refrigeration temperature and their number increases during cold storage and makes the food worse by producing exoenzymes such as protease, lipase etc.For searching of these psychrotrophs, milk and fermented milk products were collected from ten different dairies of Nasik region in Maharashtra and analyzed for total psychrotrophic count. Two psychrotrophic bacterial species that were present in milk and fermented milk products belongs to Genus Pseudomonas. These species of Pseudomonas were further identified as Pseudomonas putida and Pseudomonas stutzeri on the basis of morphological, biochemical and physiological features as well as 16S rRNA sequencing. The base sequences of these isolates were submitted to NCBI GenBank to assign the accession number. They were also analyzed for protease and lipase production at refrigeration temperature. Pseudomonas putida showed both proteolytic as well as lipolytic activity whereas Pseudomonas stutzeri showed only lipolytic activity. Both the enzymes were estimated by qualitative as well as by quantitative methods.


Deore M.K.,Science and Commerce College | Jain G.H.,Kthm College
International Journal of Nanoparticles | Year: 2014

Zinc oxide (ZnO) nanomaterial was synthesised by hydrothermal method. The formation of ZnO nanoparticles were confirmed by X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and UV studies. XRD analysis confirmed the powder to be ZnO with wurtzite structure, with crystallite size ranging from 5 to 25 nm. Observation from TEM images confirmed that the grains were nearly hexagonal rod type in nature with sizes from 22 to 56 nm. The thick films of nano ZnO were prepared by screen-printing technique in desired pattern. The surface morphology of the films was studied by scanning electron microscopy (SEM). The gas sensing performance of the materials have been investigated for various interfering gases such as CO, Cl2, NH3 and H2S etc at operating temperature varying from 50°C to 400°C. The results indicate that the nano ZnO material thick film showed much better gas response than the usual ZnO materials to H2S gas (100ppm) at 250°C. The nanoshaped hexagonal rod would improve the sensitivity and selectivity of the sensors. The selectivity, response and recovery time of the sensor were measured and presented. ZnO nanomaterial is excellent potential candidates for gas sensors. © 2014 Inderscience Enterprises Ltd.


Doke K.,University of Pune | Khan E.,University of Pune | Gaikwad V.,Kthm College | Gaikwad V.,University of Pune
Journal of Dispersion Science and Technology | Year: 2013

The present study investigates biosorption diffusion mechanism for the removal of toxic hexavalent chromium from aqueous solution using powdered cotton stalk an agricultural waste biomass. The effects of pH, temperature, adsorption isotherms, adsorption kinetics, and thermodynamic on chromium biosorption were investigated. The results showed that a maximum removal efficiency of 95% was achieved at pH 2. The pH at zero point charge (pHzpc) on biosorbent surface was 4.3. The adsorption kinetics showed that the pseudo-second order rate expression fitted well the biosrption process. The equilibrium isotherm was measured experimentally and results were analyzed by Langmuir, Freundlich, and Temkin isotherms using linearized correlation coefficients. The significant parameters for isotherms were determined. The Langmuir adsorption isotherm relative to two other isotherms was found to fit the equilibrium data best for chromium adsorption. Thermodynamic studies reveal that the biosorption of Cr(VI) on cotton stalk was endothermic, spontaneous and occurs with increase in disorder at solid-liquid interface. Adsorption diffusion kinetic was further analyzed and showed that biosorption mechanism was totally controlled by intraparticle diffusion mechanism. © 2013 Copyright Taylor and Francis Group, LLC.


Gaikwad N.,Indian Defence Institute of Advanced Technology | Bhanoth S.,Indian Defence Institute of Advanced Technology | More P.V.,Indian Defence Institute of Advanced Technology | Jain G.H.,Kthm College | Khanna P.K.,Indian Defence Institute of Advanced Technology
Nanoscale | Year: 2014

Simultaneous in situ reduction of hexachloroplatinic acid by the amine group in the pyrrole monomer and oxidation of pyrrole to form polypyrrole (PPy) was examined. The reactions were performed at various temperatures to understand the degree of reduction of platinum precursor as well as doping of polypyrrole with Pt(ii) chloro-complex. Spectroscopic images revealed different morphologies for the Pt/PPy nano-composite prepared at various temperatures. The as-prepared Pt/PPy nano-composite samples were tested for their ability to sense liquefied petroleum gas (LPG) which resulted in excellent sensing at relatively low temperature. The porous nature and ohmic contact between the PPy and platinum nanoparticles makes the as-prepared Pt/PPy nano-composite highly useful for sensors as well as electronic applications. © 2014 The Royal Society of Chemistry.


Bobade V.D.,P.A. College | Patil S.V.,P.A. College | Gaikwad N.D.,Kthm College
Journal of Chemical Research | Year: 2012

A series of new thiosubstituted 1,3,4-oxadiazole derivatives of coumarin were synthesised by reaction of 7-((5- mercapto-1,3,4-oxadiazol-2-yl)methoxy)-4- methyl-2H-chromen-2-one and α-haloketones by grinding, in solvent-free conditions at room temperature. The reaction was also carried out in ethanol in reflux conditions. Shorter reaction time and better yields were observed in solvent-free conditions. The structures of the compounds were characterised by IR, NMR, elemental analysis and mass spectral data. The synthesised compounds were evaluated for their in vitro antimicrobial screening on different strains of bacteria and fungi. Most of the compounds showed good to moderate activities.


Gaikwad N.D.,Kthm College | Patil S.V.,P.A. College | Bobade V.D.,P.A. College
European Journal of Medicinal Chemistry | Year: 2012

In continuation of our work on antimicrobial agents, a number of hybrid molecules 4a-y containing thiazole and triazole pharmacophores were designed and synthesized. The structure of the compounds was established by IR, NMR, MS and CHN analysis. All the synthesized compounds were tested for qualitative (Zone of inhibition) and quantitative (MIC) antimicrobial activities against four pathogenic bacteria Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa and two pathogenic fungi Candida albicans and Aspergillus niger. Of all the synthesized compounds screened, most of them show potent antimicrobial activity against Gram positive and Gram negative bacteria as well as the fungi species. © 2012 Elsevier Masson SAS. All rights reserved.


Gaikwad N.D.,Kthm College | Patil S.V.,P.A. College | Bobade V.D.,P.A. College
Bioorganic and Medicinal Chemistry Letters | Year: 2012

A series of novel hybrid molecules 4a-y containing thiazole and benzotriazole templates were designed and synthesized. The structures of the synthesized compounds were elucidated by IR, 1H NMR, 13C NMR and mass spectral data. All the synthesized compounds were tested for their antimicrobial activity (zone of inhibition) against Gram-positive, Gram-negative strains of bacteria as well as fungal strains. After that minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs) of all the synthesized compounds were determined. The investigation of antimicrobial screening data revealed that most of the tested compounds showed moderate to good microbial inhibitions. © 2012 Elsevier Ltd. All rights reserved.


Gaikwad N.D.,Kthm College | Patil S.V.,P.A. College | Bobade V.D.,P.A. College
Journal of Heterocyclic Chemistry | Year: 2013

A series of new 1-[4-(2,3,4-substituted-phenyl) thiazol-2-yl]-3-(2,3,4- substituted-phenyl)-1H-pyrazole-4-carbaldehyde (4a-m), 4-[4-(4-substituted- phenyl) thiazol-2-yl]-3-(4-substituted-phenyl)-1-phenyl-1H-pyrazole (7a-i), 4-[4-(4-substituted phenyl)thiazol-2-yl]-1-phenyl-1H-pyrazol-3-amine (10a-g) have been synthesized by using Vilsmeier Haack formylation and Hantzsch reaction in high yield. All the synthesized compounds were tested qualitative (Zone of inhibition) and quantitative antimicrobial activities (MIC). Most of the synthesized compounds showed potent antimicrobial activity against gram positive and gram negative bacteria as well as fungi species. © 2013 HeteroCorporation.


Shinde S.D.,Kthm College | Gaikwad V.B.,Kthm College | Patil G.E.,Commerce and Science College | Kajale D.D.,Commerce and Science College | Jain G.H.,Commerce and Science College
International Journal of Nanoparticles | Year: 2012

Nanostructured ZnO powder was synthesised by a simple chemical route. The material was characterised by XRD, UV, SEM and TEM techniques. XRD and TEM studies confirm that a nanostructured ZnO is obtained by using simple chemical route. The nanopowder was fired at an optimised temperature of 200°C for 1/2 h. The prepared powder had crystallite size in the range from 19 to 41 nm. Thick films of synthesised ZnO powder were prepared by screen printing technique. The gas sensing performances of these films for various gases were tested. Films showed highest response to H 2S (100 ppm) gas at 300°C temperature with poor responses to others. The quick response and fast recovery are the main features of this sensor. The effects of microstructure, operating temperature and gas concentration on the gas response, selectivity, response time and recovery time of the sensor in the presence of H 2S gas and others were studied and discussed. Copyright © 2012 Inderscience Enterprises Ltd.


Pethkar A.V.,Government Institute of Science | Bhagat A.P.,KTHM College
Materials Science Forum | Year: 2013

India is one of the largest producer and exporter of dyestuff among developing countries. Large amount of dyes is lost in industrial effluents during production and industrial use. Persistence of dyes in the environment, their non-biodegradability and toxicity necessitate urgent steps to develop environment-friendly approaches to remove them from wastewaters. The present work describes attempts to use iron-based catalytic nanoparticles (NPs) for removal and degradation of Acid Black 1, one of the notoriously toxic dyes. A rapid screening assay was developed in order to select NPs possessing reducing properties. Methylene blue (MB) dye (0.1mg/L, 250μl) was exposed to different preparations of NPs (7mg) for 30 minute in a 96-well microtiter plate and read at 630 nm using an ELISA reader. Iron sulphide nanoparticles (FeSNPs) stabilized by a plant biopolymer (BP) could remove >95% MB and were selected for further studies. The FeSNPs could remove Acid Black 1 efficiently (73.8%) with specific dye removal capacity of 19.7 mg/g NPs. FeSNPs were immobilized in alginate beads (average diameter 3.45 mm) and packed in polypropylene columns (22.5 cm long, 3 cm i.d.) having bed volume of 42 ml. Acid black 1 solution (20 mg/L) was passed through the columns at predetermined flow rates in up-flow mode using peristaltic pump. The mass transfer kinetics were favourable at a flow rate of 2.2 ml/min (mass transfer coefficient 9.38×10-5 L.mg-1.min-1), and 120 bed volumes of the solution could be treated with efficiency exceeding 90%. Analysis of column effluent by UV-visible and FTIR spectroscopy revealed that removal of dye from solution was due to reductive degradation. © (2013) Trans Tech Publications, Switzerland.

Loading KTHM College collaborators
Loading KTHM College collaborators