PubMed | Istanbul Education and Research Hospital, Istanbul University, Krkkale University Krkkale and Carsamba Internal Medicine Center
Type: Journal Article | Journal: Human & experimental toxicology | Year: 2016
In this study, we investigated the alterations of matrix metalloproteinase (MMP) and tissue inhibitors of metalloproteinases (TIMPs), acute inflammation, and oxidative damage in the circulatory system and the intestine in response to mesenteric ischemia/reperfusion (I/R).Twenty-one rats were divided randomly into the following three groups (n = 7 in each group): a sham group (CG), an ischemic group (IG), and an I/R group (I/RG). MMP-9, TIMP-1, and myeloperoxidase (MPO) were measured using the enzyme-linked immunosorbent assay method, and lipid peroxidation (quantified as thiobarbituric acid reactive substances (TBARS) content), ischemia-modified albumin, the prooxidant-antioxidant balance (PAB), and ferric-reducing antioxidant power (FRAP) were measured spectrophotometrically. The degree of intestinal injury was evaluated according to the Chiu scoring system.A significant difference between the mean serum TIMP-1 and MMP-9 levels and the alanine transaminase activity was found among the groups. Compared with the I/RG group a significant difference in the mean tissue MMP-9, MPO, and TBARS levels in addition to the PAB and FRAP was found between the CG and IG groups. The level of MMP-9 also demonstrated a strong, positive, and valid correlation with the TBA-RS levels. A significant morphological change was observed in both the IG and the I/RG groups. The degree of intestinal injury was more severe in the I/R group and was characterized by either villous denudation or villous loss.These results suggest that MMP-9, TIMP-1, MPO, and oxidative stress may be important in the intestinal injury development that is induced by acute mesenteric I/R in a rat model. MMP-9 overexpression may increase the extent of intestinal villous loss, particularly when MMP-9 is upregulated by the TBARS present in the intestinal injury.
PubMed | Krkkale University Krkkale
Type: Journal Article | Journal: The Journal of sports medicine and physical fitness | Year: 2015
This study examined the changes in hydration status, body composition and body mass alterations during the camping period a pre world championship, World (N.=14) and National (N.=38) senior wrestlers (N.=52) preparing for the World Championships.Urine specific gravity, conductivity and color measurements with, body composition analyses were conducted at the beginning of the camp and the end of the camp.In general, no significant differences were obtained between the world and national wrestlers in body mass and fat percentage (83.320-84.918 kg; 126%-11.24%, respectively). However, a significant difference was detected in the changes of body mass between groups (world decreased [8001 g] - national increased [6001 g] [P<0.05]). Body fat percentage was also observed to have a similar bias with a decrease and increase, but the changes were not significantly different (P>0.05). No significant differences (P>0.05) were observed between pre- and post-test values of urine specific gravity, conductivity or color for both national team wrestlers.Wrestlers achieved to adjust to weight category in a close of period through competition weigh in. Dehydration training programs should be sustained during the whole camp seasons including the last days before the weighing process for matches.