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Jyothi B.,Krishna Teja Pharmacy College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Objectives: The present study was undertaken for investigating the protective effect of ethanolic extract of Mirabilis Jalapa Linn leaves on anti-tubercular drugs induced hepatotoxicity. Methods: Anti-tubercular drugs were used to induce hepatotoxicity in rats. Silymarin was used as standard drug (100 mg/kg p.o.). Ethanolic extract of leaves of Mirabilis Jalapa Linn (250 & 500 mg/kg p.o.) was administered along with one hour prior administration of anti-tubercular drugs once daily for 35 days. Results: Liver biomarkers such as SGOT, SGPT, ALP, TB, total cholesterol were elevated and total HDL were reduced on anti-tubercular drugs administration. The treatment of ethanolic extract of Mirabilis Jalapa Linn leaves 250 mg/kg and 500 mg/kg with anti-tubercular drugs were significantly reduced liver biomarker enzymes. Antioxidant parameters such as SOD, CAT, GSH, GPx and GRx were suppressed and increased TBARs levels in anti-tubercular drugs administration but restored these antioxidant levels in the treatment of ethanolic extract of Mirabilis Jalapa Linn leaves at a dose of 250 mg/kg and 500 mg/kg. Conclusion: The result of the present study was indicated that Mirabilis Jalapa Linn leaves showed protective effect on hepatotoxicity induced by anti-tubercular drugs.


Sekhar M.C.,Gurram Balanarasaiah Institute of Pharmacy | Sekhar M.C.,Jawaharlal Nehru Technological University | Jaya Chandra Reddy P.,Krishna Teja Pharmacy College
Molecular and Cellular Biochemistry | Year: 2012

Dyslipidemia is common in patients with type 2 diabetes. Statins are used as the first choice in treatment of diabetic dyslipidemia. Atorvastatin represents a first-line treatment option, alongside other hydroxyl methylglutaryl coenzyme A reductase inhibitors. Repaglinide is a short-acting, oral, insulin secretagogue that is used in the treatment of type 2 diabetes mellitus. Both the category of drugs undergo extensive metabolism with cytochrome enzyme system. This may lead to drug-drug interaction problems with altered repaglinide activity which is cautious. Repaglinide/atorvastatin/ atorvastatin + repaglinide were administered orally to normal, diabetic rats, and to normal rabbits. Blood samples were collected at different time intervals and were analyzed for blood glucose by GOD-POD method using commercial glucose kits and repaglinide estimation in plasma by HPLC method. Diabetes was induced by alloxan 100 mg/kg body weight administered by I.P route. In the presence of atorvastatin, repaglinide activity was increased and maintained for longer period in diabetic rats compared with repaglinide matching control. The present study concludes co-administration of atorvastatin was found to improve repaglinide responses significantly in diabetic rats and improved glucose metabolism of atorvastatin played an important role and increased repaglinide levels by competitive CYP 3A4 enzyme inhibition by atorvastatin could be added advantage for anti hyperglycemic activity. © Springer Science+Business Media, LLC. 2011.


Mohan Krishna L.,Krishna Teja Pharmacy College | Jayachandra Reddy P.,Krishna Teja Pharmacy College | Jaya Sankar Reddy V.,Krishna Teja Pharmacy College | Prasada Rao K.V.S.,Rahul Institute of Pharmaceutical science
Rasayan Journal of Chemistry | Year: 2011

Three simple and sensitive visible spectrophotometric methods (M 1, M2, and M3) have been described for the estimation of Ceftazidime (CTZ). The methods that are based on the formation of radical anion with the involvement of basic nitrogen in CTZ (donor) and quinones [2,3-dichloro-5,6-dicyano-p-benzoquinone(DDQ), chloranilic acid (DHQ), 2,3,5,6-tetrachloro-p-benzoquinone (TQ)] (acceptor). The variable parameters in all these methods have been optimized and the chemical reactions involved are presented. The results obtained in the three methods are statistically validated and recoveries range from 99.7 to 101.3%. Common excipients used in additives in pharmaceutical preparations do not interfere in die proposed methods. © 2011 RASAYAN. All rights reserved.


Krishna L.M.,Krishna Teja Pharmacy College | Reddy P.J.,Krishna Teja Pharmacy College | Rao K.V.S.P.,Rahul Institute of Pharmaceutical science
Asian Journal of Chemistry | Year: 2011

Simple spectrophotometric methods (A-C) for the assay of aripiprazole (APZ) based on the formation of its complex with alkaloidal precipitants are described. Aripiprazole under quantitative precipitation in the form of molecular complexes with iodine (method A), ammonium molybdate (method B) or phosphomolybdic acid (method C) when used in excess. In addition to precipitation reactions, colour reactions have also been combined to estimate aripiprazole. They are based on the colour formation with either unreacted precipitant of the filtrate (I2) or released precipitant from the molecular complex (method B or C) with chromogenic reagent such as P-N-methyl amino phenol sulphate-sulphanilic acid (method A), potassium thiocyanate (method B), cobalt nitrate Co(II)-disodium salt of ethylene diamine tetra acetic acid complex (method C).


Sangeetha G.,Krishna Teja Pharmacy College | Jubaitha Begum M.,Krishna Teja Pharmacy College | Reddemma S.,Krishna Teja Pharmacy College | Rajendra Y.,Krishna Teja Pharmacy College
International Journal of Pharmacy and Technology | Year: 2011

Targeted drug delivery into the colon is highly desirable for local treatment of a variety of bowel diseases such as ulcerative colitis, Crohn's disease, amoebiosis, colonic cancer, local treatment of colonic pathologies, and systemic delivery of protein and peptide drugs. To achieve successful colon targeted drug delivery, a drug need to be protected from degradation, release and absorption in upper portion of the GI tract and then to be ensured abrupt or controlled release in the proximal colon. This review is focused on the merits and demerits, novel approaches in the colon targeted drug delivery, clinical evaluation techniques and some information on the marketed dosage forms.


Shaik S.,Krishna Teja Pharmacy College | Rajendra Y.,Krishna Teja Pharmacy College | Jaya Chandra Reddy P.,Krishna Teja Pharmacy College
International Journal of Pharmacy and Technology | Year: 2012

Mirabilis Jalapa L. is a perennial herbal medicinal plant, has a long traditional uses. Various constituents are found in different parts of the plants. The active chemical constituents reported from this plant are alkaloids, flavonoids, phenols, glycosides, tannins, saponins, lignins, carbohydrates. In conventional medicine, the plant is used in treatment of skin diseases, carminative, cathartic, purgative, stomachic, tonic and vermifuge properties, anti-dysenteric, anti-parasitic, wound healer, digestive stimulant. Its various extracts reported number of pharmacological activities such as anti-diabetic, anti-inflammatory, anti-oxidative, antibacterial, anti-microbial, anti-fungal, anti-spasmodic, antinociceptive, anti-viral, diuretic, anthelmentic and urinary tract disorder. The present review is therefore an effort to give a detailed survey of the literature on its pharmacognosy, phytochemical and its traditional uses along with special emphasis given on pharmacological activities.


Reddy M.V.,Krishna Teja Pharmacy College | Aruna G.,Krishna Teja Pharmacy College | Parameswari S.A.,Krishna Teja Pharmacy College | Banu B.H.,Krishna Teja Pharmacy College | Reddy P.J.,Krishna Teja Pharmacy College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2015

Objective: According to Food Safety and Standards Authority of India (FSSAI) the preservatives, sodium benzoate and potassium sorbate belong to permitted class II preservatives. The aim of this study is to determine the concentration levels of these preservatives in food products that are consumed by school children and to assess the chronic dietary exposure by conducting the Total Diet Study (TDS). Methods: The quantitative determination was carried out by UV spectrophotometer. The absorbance for sodium benzoate and potassium sorbate were measured at 228 and 250 nm respectively. The 24-hour diet recall method was used to estimate the amount of food ate in last 24 hours. For estimation of preservative exposure dietary modelling techniques were utilized which combine the amount of preservative concentration present in that food with the amount of food consumed. Then the dietary exposure was assessed by considering the Acceptable Daily Intake (ADI). Results: The results include chemical concentration levels of the foods analyzed as well as estimated dietary exposures and contributions to the exposure from different foods. The obtained mean concentration of sodium benzoate was found to be425 ppm for sauces, 161 ppm for pickles and 80 ppm for soft drinks. Potassium sorbate was found to be 130 ppm for fruit juices,302 ppm for jellies and 380 ppm for jams. The highest mean dietary exposure for both the preservatives was observed in children of 2-7 years age group, the percentage exposure of sodium benzoate was 33% of the ADI and potassium sorbate was 17 % of the ADI. Conclusion: This study can enlighten the public on the consumption of preservative containing food products within the limit and encourages to eating fresh preservative free foods. © 2015, International Journal of Pharmacy and Pharmaceutical Science.


Shaik S.,Krishna Teja Pharmacy College | Dhanalakshmi M.,Krishna Teja Pharmacy College | Jayasree T.,Krishna Teja Pharmacy College
International Journal of Pharmacy and Technology | Year: 2012

Mirabilis Jalapa. Linn (Nyctaginaceae) is an herbaceous medicinal plant used to treat wound healing, stomachic, liver problems, dysentery, diarrhea, muscular pain and abdominal colic in India and other Asian countries. Acetaminophen (APAP) is a commonly used antipyretic and analgesic agent which, at high doses, causes liver and kidney necrosis in man and animals. The aim of this study was to investigate the nephroprotective and antioxidant activities of ethanol extract of MJ at two dose levels of 250 and 500 mg/kg B/W on acetaminophen (APAP) induced toxicity in male albino rats. APAP significantly increased levels of serum urea, hemoglobin (Hb), total leukocyte count, packed cell volume, creatinine, DLC, and mean corpuscular volume, raised body weight, and reduced levels of neutrophils, mean corpuscular Hb content, mean corpuscular hematocrit, granulocytes, uric acid, and platelet Concentration. Mirabilis Jalapa. Linn (MJ) inhibited the hematological effects of APAP. MJ significantly increased activities of renal superoxide dismutase, catalase, glutathione, and glutathione peroxidase and decreased malondialdehyde content of APAP treated rats. Apart from these, histopathological changes also showed the protective nature of the MJ extract against APAP induced necrotic damage of renal tissues. In conclusion it was observed that the ethanol extract of MJ conferred nephroprotective and antioxidant activities by histopathological and biochemical observations against APAP induced renal damage in rats.


Dhanalakshmi M.,Krishna Teja Pharmacy College | Jayasree T.,Krishna Teja Pharmacy College | Shaik S.,Krishna Teja Pharmacy College
International Journal of Pharmacy and Technology | Year: 2012

In the present study was carried out to evaluate the protective effect of ethanolic extract of leaves of Michelia champaca against Freund.s adjuvant induced arthritis in rats. The ethanolic extract was administered orally at dose of 250 and 500 mg/kg body weight. Indomethacin at dose of 10 mg/kg body weight was used as standard drug. The paw volume was measured on days 4, 8, 14, and 21. At the end of day 21 the blood was collected from retro-orbital route to all the groups of animals and various hematological parameters such as hemoglobin content, total WBC, RBC and erythrocyte sedimentation rate were estimated. The results indicate that at dose of 500 mg/kg body weight of ethanolic extract protects rats against the primary and secondary arthritic lesions, body weight changes and hematological perturbations induced by CFA. Daily treatment of rats with ethanolic extract, standard drug Indomethacin effectively inhibits paw edema in rats. The extract significantly (p< 0.01) altered the parameters which were estimated when compared to control group rats.At the end of studies. The phytochemical analysis of extracts revels the presence of sterols, flavonoids, phenols, tannins and saponins. However additional clinical investigations are needed to prove the efficacy of Michelia champaca L.in the treatment of various immuno-inflammatory disorders.

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