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Vetrov S.Ya.,RAS Kirensky Institute of Physics | Pyatnov M.V.,Krasnoyarsk State Medical University | Timofeev I.V.,RAS Kirensky Institute of Physics
Optics Letters | Year: 2014

The light transmission spectrum has been calculated for a "cholesteric liquid crystal-phase plate-metal" structure. It is shown that the system can have an isolated waveguide surface mode with characteristics efficiently controllable by external fields acting on the cholesteric. The degree of localization of surface modes and the transmission coefficients have been found to differ considerably for the light of different polarizations. © 2014 Optical Society of America.


Labib M.,University of Ottawa | Zamay A.S.,University of Ottawa | Zamay A.S.,Krasnoyarsk State Medical University | Berezovski M.V.,University of Ottawa
Analyst | Year: 2013

A novel attempt was made to develop a disposable multifunctional sensor for analysis of vaccinia virus (VACV), a promising oncolytic agent that can replicate in and kill tumor cells. Briefly, we developed aptamers specific to VACV that were negatively selected against human serum as well as human and mouse blood to be further utilized for viral analysis directly in serum and blood. In addition, the aptamers were negatively selected against heat-inactivated VACV to enable them to distinguish between viable and nonviable virus particles. The selected aptamers were integrated onto an electrochemical aptasensor to perform multiple functions, including quantification of VACV, viability assessment of the virus, and estimation of the binding affinity between the virus and the developed aptamers. The aptasensor was fabricated by self-assembling a hybrid of a thiolated ssDNA primer and a VACV-specific aptamer onto a gold nanoparticles modified screen-printed carbon electrode (GNPs-SPCE). Square wave voltammetry was employed to quantify VACV in serum and blood within the range of 150-900 PFU, with a detection limit of 60 PFU in 30 μL. According to the electrochemical affinity measurements, three virus specific aptamer clones, V-2, V-5, and V-9 exhibited the highest affinity to VACV. Furthermore, flow cytometry was employed to estimate the dissociation constants of the clones which were found to be 26.3, 40.9, and 24.7 nM, respectively. Finally, the developed aptasensor was able to distinguish between the intact virus and the heat-inactivated virus thanks to the tailored selectivity of the aptamers that was achieved via negative selection. This journal is © The Royal Society of Chemistry.


Popelysheva A.E.,Krasnoyarsk State Medical University
Diabetes Mellitus | Year: 2015

Aim. The haemodynamics of patients with diabetes mellitus 2 during acute ischaemic stroke and their connection with the recovery of neurological deficit has been inadequately studied. Materials and Methods. We studied the blood pressure dynamics of 183 patients with mild-to-moderate acute ischaemic stroke receiving antihypertensive therapy, depending on whether or not they had type 2 diabetes mellitus. Results. In patients with diabetes, high blood pressure persisted during the first week, mainly because of the diastolic index, and they had a more rapid decline in blood pressure in the second week than those without diabetes. However, reaching the target blood pressure of <160/100 mmHg was faster in patients with diabetes, regardless of the intensity of antihypertensive therapy. Although diabetes significantly correlated with the efficiency of recovery of neurological deficit, it also showed a tendency to frequent functional impairment in patients with diabetes than those without diabetes. Conclusions. The results suggest that patients with diabetes and hypertension require less aggressive treatment, particularly in the second week of hospitalization. This may affect the efficiency of recovery of functional deficits. Further study of blood pressure dynamics and their relation to functional recovery of neurological deficit is required during the acute period after an ischaemic stroke in patients with diabetes.


Tohidpour A.,Krasnoyarsk State Medical University
Microbial Pathogenesis | Year: 2016

Helicobacter pylori has been described as the main etiologic agent of gastric cancer, causing a considerable rate of mortality and morbidity in human population across the world. Although the infection mainly begins asymptomatically, but simply develops to peptic ulcer, chronic gastritis, lymphoma of the gastric mucosa and eventually adenocarcinoma. The major pathological feature of H. pylori infection is due to the activity of the cytotoxin-associated gene A (CagA), a 125-140 kDa protein encoded by the cag pathogenicity island (cagPAI). CagA is also known as the first bacterial onco-protein, ranking the H. pylori-mediated adenocarcinoma as the second most deadly cancer type worldwide. Upon cytoplasmic translocation CagA undergoes interacting with numerous proteins in phosphorylation dependant and independent manners within the gastric epithelial cells. The profound effect of CagA on multiple intracellular pathways causes major consequences such as perturbation of intracellular actin trafficking, stimulation of inflammatory responses and disruption of cellular tight junctions. Such activities of CagA further participate in development of the hummingbird phenotype and gastric cancer. This review is sought to provide a structural and functional analysis of the CagA protein with focus on demonstrating the molecular basis of the mechanism of CagA intracellular translocation and its interaction with intracellular targets. © 2016 Elsevier Ltd.


Labib M.,University of Ottawa | Zamay A.S.,University of Ottawa | Zamay A.S.,Krasnoyarsk State Medical University | Muharemagic D.,University of Ottawa | And 4 more authors.
Analytical Chemistry | Year: 2012

The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores. © 2012 American Chemical Society.


Aksenenko M.B.,Krasnoyarsk State Medical University | Kirichenko A.K.,Krasnoyarsk State Medical University | Ruksha T.G.,Krasnoyarsk State Medical University
Pathology Research and Practice | Year: 2015

Melanoma is one of the aggressive cancer types causing the majority of deaths in skin cancer patients. Mutational screening of the tumor revealed a number of driver mutations in oncogenes which enabled melanoma classification into a few molecular subtypes. BRAF is a key component of mitogen-activated kinase pathway; its activating mutation leads to accelerated melanoma cells proliferation, invasion and survival. Somatic mutations in BRAF were reported in various malignancies, including thyroid cancer, colorectal cancer and melanoma. Specific features of BRAF-positive tumors could have clinical implications as mutational alterations may have an impact on the biological behavior of the tumor and prognosis of the disease. In the present study, the frequency of BRAF V600E mutation was evaluated in Russian patients with melanocytic lesions, of which 41.25% were primary melanoma and 60% were melanocytic nevi. Melanoma patients with trunk localization were of younger age in the BRAF-positive group as compared with BRAF-negative patients. Immunohistochemical evaluations of Ki-67 expression, as well as matrix metalloproteinase-2, -9, were found to be equal in BRAF-positive and BRAF-negative tumors. MMP-2/MMP-9 immunoreactivity was observed in stromal and/or melanocytic cells both in melanoma and nevi patients. Besides tumor cells, MMP-9 expression was observed in lymphocytes in 27.2% of BRAF-positive and in 19.1% of BRAF-negative patients. Histopathological prognostic markers (Breslow thickness, mitotic index, ulceration, tumor infiltrating lymphocytes pattern) did not show any differences depending on BRAF V600E mutational status. The frequency of BRAF-positive melanomas in Russian cohort is similar to other Caucasian population rates. BRAF V600E mutation harboring tumors are more often observed in younger patients without specific features of morphological prognostic factors. © 2015 Elsevier GmbH.


Motorina A.V.,Krasnoyarsk State Medical University
European Journal of Cancer Prevention | Year: 2016

The melanocortin 1 receptor is a Gs protein-coupled receptor implicated in melanogenesis regulation. The receptor gene is highly polymorphic, which accounts for the association of several of its single-nucleotide polymorphisms (SNPs) with an increased risk of melanoma. The present study aimed to evaluate the distribution of melanocortin 1 receptor gene variants R151C, R160W, and D294H within the Russian population of Eastern Siberia and its association with melanoma development. Melanoma patients (n=95) admitted to Krasnoyarsk Territorial Oncological Center and healthy controls (n=334) were enrolled in the study. A clinical examination of patients was performed to evaluate the phenotypic features of melanoma patients. SNPs were analyzed by real-time PCR. Clinical examination indicated a more frequent occurrence of fair skin type, blue eyes, blonde and red hair, and more frequent localization of freckles on the neck, trunk, and extremities in the melanoma group of patients. The R151C melanocortin 1 receptor gene variant was found in 18% of melanoma patients and associated with an increased likelihood of melanoma development (odds ratio=6.4; 95% confidence interval: 2.8–14.3; P=0.0001). The two remaining variant alleles of the melanocortin 1 receptor gene occurred with low frequency both in controls and in the melanoma group. The R160W SNP was identified neither in controls nor in melanoma patients. The D294H heterozygous variant was observed in 0.3% of individuals in the control group and in 1.1% of the patients in the melanoma group. Such an asymmetric distribution of the melanocortin 1 receptor within red hair color genotypes in the population under study compared with other populations may be because of Russian genetic homogeneity. Carriers of the mutant R151C allele should exercise caution in terms of exposure to the sun to avoid the risk of melanoma development.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/ Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.


Salmina A.B.,Krasnoyarsk State Medical University | Inzhutova A.I.,Krasnoyarsk State Medical University | Malinovskaya N.A.,Krasnoyarsk State Medical University | Petrova M.M.,Krasnoyarsk State Medical University
Journal of Alzheimer's Disease | Year: 2010

Current theories state that Alzheimer's disease (AD) is a vascular disorder that initiates its pathology through cerebral microvascular abnormalities. Endothelial dysfunction caused by the injury or death of endothelial cells contributes to progression of AD. Also, functional relationships between neurons, glial cells, and vascular cells within so-called neurovascular unit are dramatically compromised in AD. Several recent studies have highlighted that endothelial cells might be the target for the toxic action of heavily aggregated proteins, glia-derived cytokines, and stimuli inducing oxidative and metabolic stress in AD brains. Here, we describe the properties of the brain endothelium that contribute to its specific functions in the central nervous system, and how endothelial-neuronal-glial cell interactions are compromised in the pathogenesis of AD. We also discuss the ways in which functioning of endothelial cells can be modulated in cerebral microvessels. Understanding of molecular mechanisms of endothelial injury and repair in AD would give us novel diagnostic biomarkers and pharmacological targets. © 2010 IOS Press and the authors. All rights reserved.


Muharemagic D.,University of Ottawa | Labib M.,University of Ottawa | Ghobadloo S.M.,University of Ottawa | Zamay A.S.,University of Ottawa | And 4 more authors.
Journal of the American Chemical Society | Year: 2012

Oncolytic viruses are promising therapeutics that can selectively replicate in and kill tumor cells. However, repetitive administration of viruses provokes the generation of neutralizing antibodies (nAbs) that can diminish their anticancer effect. In this work, we selected DNA aptamers against the antigen binding fragment (Fab) of antivesicular stomatitis virus polyclonal antibodies to shield the virus from nAbs and enhance its in vivo survival. For the first time, we used flow cytometry and electrochemical immunosensing to identify aptamers targeting the Fab region of antibodies. We evaluated the aptamers in a cell-based infection assay and found that several aptamer clones provide more than 50% shielding of VSV from nAbs and thus have the potential to enhance the delivery of VSV without compromising the patient's immune system. In addition, we developed a bifunctional label-free electrochemical immunosensor for the quantitation of aptamer-mediated degree of shielding and the amount of vesicular stomatitis virus (VSV) particles. Electrochemical impedance spectroscopy was employed to interrogate the level of VSV in a linear range from 5 × 104 to 5 × 106 PFU mL-1 with a detection limit of 104 PFU mL-1. © 2012 American Chemical Society.


Davydov E.L.,Krasnoyarsk State Medical University
Advances in Gerontology | Year: 2013

The article presents data on the features of nervous and depressive states in elderly and advanced-age patients with arterial hypertension. Age and gender differences of these problems are discussed. Patients of senior age groups have a normal level of nervousness on a scale of situational nervousness, and women of advanced age and patients of the control group have an increased level of nervousness on a scale of personal nervousness. Gender differences on a scale of personal nervousness are revealed. Frustrations of the depressive range are noted in assessing depressive status in all age and gender groups. © 2013 Pleiades Publishing, Ltd.

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