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Osijek, Croatia

Klir Z.,Kralja petra Svacica 1d | Antunovic Z.,Kralja petra Svacica 1d
Agriculturae Conspectus Scientificus | Year: 2013

The aim of the present study was to determine differences of amount and composition of milk between conventional and organic systems in Istrian pramenka breed of sheep. Sum of the data included 838 conventional and 9238 organic samples of ewes' milk. Each farm is classified as organic from the date of entering in organic production. Before that date farms didn't show characteristics of organic production, therefore they were classified as conventional. The results of the present study included higher amount of morning (353.10 g) and evening milk (361.39 g) in conventional compared to morning (342.76 g) and evening milk (345.69) in organic system, significantly influenced by number of lactation (P<0.001), breeding system and breeder interaction (P<0.001). Higher content of fat in conventional system was significantly influenced by breeding system (P<0.05), breeding system and breeder interaction (P<0.001), number of lactation (P<0.01) and lactation length (P<0.001). Lower protein content in conventional system was influenced significantly by breeding system and breeder interaction (P<0.001), litter size (P<0.05) and lactation length (P<0.001). Content of lactose was higher in organic system (3.93%) compared to conventional system (3.84%) significantly influenced by breeding system (P<0.01) and number of lactation (P<0.001). According to results, differences between conventional and organic systems are the mostly influenced by breeder in Slovenia.

Raspudic E.,Kralja petra Svacica 1d | Sarajlic A.,Kralja petra Svacica 1d | Ivezic M.,Kralja petra Svacica 1d | Majic I.,Kralja petra Svacica 1d | And 2 more authors.
Poljoprivreda | Year: 2013

The aim of this study was to determine the effectiveness of a chemical treatment against larvae of the European corn borer (Ostrinia nubilalis Hubner). The experiment was set up in 2010 and 2011 in Čepin (eastern Croatia in two treatments: control treatment and insecticide treatment). The trial involved two hybrids of FAO group 400: PR37N01 and PR37F73. Biology of pests was monitored in order to determine population size and larvae development stage as well as the optimal time of insecticide application. After determination of thresholds, maize was treated with chemical formulations of active substance dimethoate. Towards the end of vegetation, length of stem damage, number of larvae in maize stalk and ear as well as grain yield were recorded by dissection of maize stalks. Statistical analysis shows that year, hybrid and chemical treatment significantly influenced the incidence of this pest and justified the use of chemical preparations with mandatory monitoring biology of this pest.

Comparison of productive parameters of two analyzed groups (the first generation of imported gilts and the gilts born on the domestic farm) was conducted on a nucleus farm that imported the gilt lines from the TOPIGS breeding program. For the purposes of the nucleus pig farm, TOPIGS gilts were imported from Netherlands. The nucleus farm where the analysis of the two groups' productive parameters was conducted imported the ancestral and grandfather lines to produce the parenting generation TOPIGS 40 sows which are bred with a Pietrain line of boars. The TOPIGS 40 sow is an animal based on A-line and B-line characterized by high fertility rate, excellent maternal characteristics, good capacity for growth, and quality meaty body. The analysis of productive parameters of the two observed groups of gilts (the first generation of 105 imported gilts and 88 gilts born on the domestic farm) resulted in significantly higher number of total born, born alive and weaned piglets after the first farrowing (P<0.001) as well as significantly higher number of total born and born alive piglets at third farrowing (P<0.05). The results of the observed qualities confirm the fact that, apart from the unquestionable genetic potential, other factors such as adaptation, accommodation conditions, microclimate, nutrition, and management have a significant effect on the increase of production results.

Vrandecic K.,Kralja petra Svacica 1d | Cosic J.,Kralja petra Svacica 1d | Jurkovic D.,Kralja petra Svacica 1d | Stankovic I.,University of Belgrade | And 3 more authors.
Plant Disease | Year: 2014

Lavandula × intermedia Emeric ex Loiseleur, commonly known as lavandin, is an aromatic and medicinal perennial shrub widely and traditionally grown in Croatia. The lavandin essential oil is primarily used in perfumery and cosmetic industries, but also possesses anti-inflammatory, sedative, and antibacterial properties. In June 2012, severe foliar and stem symptoms were observed on approximately 40% of plants growing in a commercial lavandin crop in the locality of Banovo Brdo, Republic of Croatia. Initial symptoms on lower leaves included numerous, small, oval to irregular, grayish brown lesions with a slightly darker brown margin of necrotic tissue. Further development of the disease resulted in yellowing and necrosis of the infected leaves followed by premature defoliation. Similar necrotic oval-shaped lesions were observed on stems as well. The lesions contained numerous, dark, sub-globose pycnidia that were immersed in the necrotic tissue or partly erumpent. Small pieces of infected internal tissues were superficially disinfected with 50% commercial bleach (4% NaOCl) and placed on potato dextrose agar (PDA). A total of 10 isolates from leaves and five from stems of lavandin formed a slow-growing, dark, circular colonies with raised center that produced pycnidia at 23°C, under 12 h of fluorescent light per day. All 15 recovered isolates formed uniform hyaline, elongate, straight or slightly curved conidia with 3 to 4 septa, with average dimensions of 17.5 to 35 × 1.5 to 2.5 μm. Based on the morphological characteristics, the pathogen was identified as Septoria lavandulae Desm., the causal agent of lavender leaf spot (1,2). Pathogenicity of one selected isolate (428-12) was tested by spraying 10 lavandin seedlings (8 weeks old) with a conidial suspension (106 conidia/ml) harvested from a 4-week-old monoconidial culture on PDA. Five lavandin seedlings, sprayed with sterile distilled water, were used as negative control. After 5 to 7 days, leaf spot symptoms identical to those observed on the source plants developed on all inoculated seedlings and the pathogen was successfully re-isolated. No symptoms were observed on any of the control plants. Morphological identification was confirmed by amplification and sequencing of the internal transcribed spacer (ITS) region of rDNA (3). Total DNA was extracted directly from fungal mycelium with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and PCR amplification performed with primers ITS1F/ITS4. Sequence analysis of ITS region revealed at least 99% identity between the isolate 428-12 (GenBank Accession No. KF373078) and isolates of many Septoria species; however, no information was available for S. lavandulae. To our knowledge, this is the first report of Septoria leaf spot of lavandin caused by S. lavandulae in Croatia. Since the cultivation area of lavandin plants has been increasing in many continental parts of Croatia, especially in Slavonia and Baranja counties, the presence of a new and potentially harmful disease may represent a serious constraint for lavandin production and further monitoring is needed.

Knezevic D.,Croatian Food Agency | Durkin I.,Kralja petra Svacica 1d | Kusec G.,Kralja petra Svacica 1d | Kralik G.,Kralja petra Svacica 1d | Jerkovic I.,Belje Inc.
Agriculturae Conspectus Scientificus | Year: 2013

Study was carried out on 82 hybrid pigs with the aim to investigate influence of g.489C>T SNP of MYOD1 on carcass traits, meat quality and on chemical composition of longissimus dorsi muscle. Slaughter was carried out at approximately 110, 125 and 150 kg live weight. Carcass lengths from os pubis to atlas and from os pubis to 1st rib, ham length (from os calcaneus to os ichium) and its circumference, pH45 and pH24 at semimembranosus and longissimus dorsi muscles and electrical conductivity were determined at slaughter line. Loin eye and fat area according to Comberg (1978), CIE L*a*b* colour coordinates, drip loss by "bag method", cooking loss and shear force were measured in laboratory at longissimus dorsi muscle. Contents of intramuscular fat, protein, moisture and collagen were determined by FoodScan™ Meat Analyser. Pig DNA was isolated from animal tissue and genotyped for C489T substitution at MYOD1 gene. Genotype frequencies were 20.73%, 36.58% and 42.68% for CC, TT and CT, respectively. C489T mutation influenced all carcass traits, where CT genotype showed preferable values in carcass lengths, ham length, its circumference and loin eye area indicating its potential use as marker for improved pig carcass traits. Influence of C489T SNP was observed for pH45 in semimembranosus muscle and both pH45 and pH24 in longissimus dorsi muscle. The same genotype showed favourable values for all three traits.

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