Jung U.-H.,Koregon Co. |
Oh J.-H.,Koregon Co. |
Kim Y.-G.,Koregon Co. |
Ahn C.-H.,Koregon Co. |
And 6 more authors.
Journal of Plant Biotechnology | Year: 2014
Using commercial radish varieties for processing, about 30% of radish was discarded due to the root shape and low purity. To raise the processing ability, we tried to develop a new variety producing H-shaped root. As another characteristic required in variety for processing is high purity, we tried to raise purity using simple sequence repeats (SSR) markers for testing seed purity in every segregating generation. To develop Male-sterile (MS) seeding parent, we crossed commercial variety of 'Gwan dong spring' and 'Gyeo ryong spring'. One elite inbred was selected as recurrent parent for the MS plant. The major horticultural traits of selected inbred line were disease resistance, late bolting, heat resistance and bright green root top color. To develop pollen parent, we crossed commercial variety of 'Tae sang king' and 'Seoul spring'. We used individual selection method to develop H-shaped hard root and disease resistant inbred. In each segregating generation, we selected one plant based on phenotype and the uniformity of selected plant was tested by SSR markers using self-pollinated seeds. In the first segregating generation, 64.6% of sib plants shared the same band in PCR amplification using ACMP-490 primer and 66.7% using cnu-316 primer. The uniformity of segregating generations using ACMP-490 and cnu-316 raised in second generation to 68.8%, 70.8%, respectively; in third generation to 93.8%, 100%; in fourth generation to 93.8%, 100%; in fifth generation to 95.8%, 100%; in sixth generation to 100%, 100%. A novel cross was made using selected MS parent and pollen parent. When we checker the horticultural traits using autumn cultivation, the novel cross variety produced H-shaped root comparing other commercial varieties and produced highly uniform radish. Thus we registered this novel cross variety as 'YR ORE' at 2013 (Registration No. 4550). © 2014 Korean Society for Plant Biotechnology.
Lee J.,Korea Research Institute of Bioscience and Biotechnology |
Yang K.,Korea Research Institute of Bioscience and Biotechnology |
Lee M.,Korea Research Institute of Bioscience and Biotechnology |
Kim S.,Korea Research Institute of Bioscience and Biotechnology |
And 9 more authors.
Process Biochemistry | Year: 2015
The aerial surfaces of plants are covered with a wax layer that serves the essential functions of limiting non-stomatal water loss and acting as protective barrier against environmental stresses. We selected two broccoli lines, bloomed (MC91) and bloomless (MC117), and analyzed their phenotypes related to cuticular wax accumulation. The total wax amount was 1.93-fold higher in MC91 leaves compared to MC117 leaves. All of the studied cuticular wax compounds were 1.07-3.79-fold higher in MC91 plants compared to MC117 plants except for the C31 alkane. The wax compositions did not essentially different between the two broccoli lines, but some compounds were found at significantly higher levels in MC91 plants compared to MC117 plants, mainly reflecting differences in C29 alkanes, C29 secondary alcohols and C29 ketones. To investigate gene regulation by bloom phenotype, we analyzed the mRNA expression patterns of various cuticular wax biosynthetic genes. Our results revealed that LACS1, KCS1, KCR1, ECR, CER3 and MAH1 were expressed more in MC91 plants compared to MC117 plants at both 3 and 10 weeks. The expression levels of the studied cuticular wax biosynthetic genes were significantly induced by drought stress, which is known to induce cuticular wax deposition. Together, these results show that the cuticular wax accumulation of broccoli is regulated by cuticular wax biosynthetic gene expression and can be affected by environmental signals. © 2015 Elsevier Ltd. All rights reserved.
Jo J.S.,Chonbuk National University |
Bhandari S.R.,Chonbuk National University |
Kang G.H.,Koregon Co. |
Lee J.G.,Chonbuk National University
Horticulture Environment and Biotechnology | Year: 2016
The aim of this research was to evaluate the profile and concentration of individual glucosinolates (GSL), and the total phenol content (TPC), total flavonoid content (TFC), ascorbic acid content, and antioxidant activity of broccoli florets and flower stalks (10 commercial cultivars, 19 F1 hybrids, and 20 inbred lines). All broccoli heads were harvested at their marketable stage, and their flower stalks and florets were subjected to phytochemical analysis. GSL, TPC, TFC, and ascorbic acid content varied significantly depending on broccoli genotype. Altogether, nine GSLs were identified, four of which (glucoraphanin, progoitrin, glucoerucin, and glucobrassicin) were the most common in both broccoli flower stalks and florets. In florets, glucobrassicin was the most abundant GSL (4.46 μmol·g-1 DW), followed by glucoraphanin (1.93 μmol·g-1 DW), whereas glucoraphanin was the most abundant in flower stalks (1.47 μmol·g-1 DW). The concentrations of total GSLs, TPC, and TFC in florets were relatively higher than those in the flower stalks, whereas the concentration of ascorbic acid was higher in the flower stalks than the florets. Almost all F1 hybrids and inbred lines exhibited higher TPC, TFC, ascorbic acid concentration, and antioxidant activities than those in the commercial cultivars. Three F1 hybrids; 5075, 5078, and 5079, and one inbred line (5308) had the highest glucoraphanin and total GSL content. Three inbred lines, 5307, 5311, and 5409 had the higher concentration of glucobrassicin and total GSLs, superior antioxidant activity with low PRO+EPI content. These results suggest that these genotype selections had desirable compositions of individual GSLs and higher nutritional value for commercialization as functional vegetables. © 2016, Korean Society for Horticultural Science and Springer-Verlag GmbH.