Korean Collection of Herbal Extract Inc.

Kongju, South Korea

Korean Collection of Herbal Extract Inc.

Kongju, South Korea
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Kim S.-J.,Kongju National University | Sancheti S.A.,Kongju National University | Sancheti S.S.,Kongju National University | Um B.-H.,Korea Institute of Science and Technology | And 3 more authors.
Acta Poloniae Pharmaceutica - Drug Research | Year: 2010

In the current era, natural products are gaining prime attention in the fields of cosmeceuticals and pharmaceuticals due to higher safety margins and biological functions, as they have a considerable amount of potential in treating different ailments. Thus, to find effective elastase and hyaluronidase inhibitors from natural resources, fifty Korean plants were screened, and the fruit of Terminalia chebula RETZIUS (Combretaceae) was selected for further structural isolation due to its potent efficacy. The methanol crude extract of the fruits showed 80% elastase and 87% hyaluronidase enzyme inhibition activities at 1 mg/mL. The crude extract, upon bioassay-directed fractionation, led to the isolation of compound 1, whose structure was found by spectral analysis to be 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG). PGG displayed significant elastase and hyaluronidase inhibitory activities with IC 50 values of 57 μg/mL and 0.86 mg/mL, respectively; also, treatment of PGG on rabbit articular chondrocytes significantly induced the type II collagen expression. Based on elastase and hyaluronidase inhibitions, and type II collagen expression, it could be suggested that PGG might have an influence on skin conditions when used cosmetically as an active anti-aging ingredient with no cytotoxicity; also, it might be beneficial in relieving painful joint conditions, and thus have relevance for treating arthritis. Therefore, it can be concluded that PGG may prove to be an active ingredient in cosmeceutical and pharmaceutical formulations, and that it definitely merits further in vivo investigations.

Sancheti S.,Kongju National University | Seo S.-Y.,Kongju National University | Seo S.-Y.,Korean Collection of Herbal Extract Inc.
Pakistan Journal of Pharmaceutical Sciences | Year: 2010

In this era, major community worldwide is suffering from diabetes type II, cancer and neurodegenerative disorders. To overcome these diseases, in the screening of Korean medicinal plants, we studied the whole plant of Boehmeria nivea (B. nivea). The methanolic leaf, stem and root extracts of B. nivea and their respective n-hexane, methylene chloride (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH) and aqueous fractions were investigated for their total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, α-glucosidase, β-glucosidase, α-galactosidase, β-galactosidase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme inhibition activities. Profound TPC and DPPH free radical scavenging activities were observed in the EtOAc and BuOH fractions of root, where the BuOH fraction showed high-pitched α-glucosidase inhibition and the EtOAc layer showed the maximum β-glucosidase inhibition. Furthermore, the leaf extract demonstrated the highest β-galactosidase inhibitory activity, but no α-galactosidase inhibition was seen in any of the plant parts. Notable BChE and moderate AChE inhibitory activity was found in whole plant. It can be suggested that whole plant of B. nivea provides a strong biochemical rationale as one of the good choices for the treatment of diabetes type II, cancer and neurodegenerative diseases (AD, etc).

Sancheti S.,Kongju National University | Bafna M.,Kongju National University | Kim H.-R.,Kongju National University | You Y.-H.,Kongju National University | And 2 more authors.
Brazilian Journal of Pharmacognosy | Year: 2011

The potential role of 80% methanolic extract of Boehmeria nivea (L.) Gaudich., Urticaceae, root in the treatment of diabetes, along with its antihyperlipidemic and antioxidant effects, was studied in streptozotocin-induced diabetic male Wistar rats. Preliminary screening of the extract revealed the presence of polyphenolics and flavonoids. The animal study was conducted with variable doses of 125, 250 and 500 mg/kg of extract for 21 days in diabetic rats. A significant effect was observed at a dose of 500 mg/kg, which was comparable to the standard drug, glibenclamide. Administration of the extract at a 500 mg/kg dose resulted in a significant reduction of fasting blood glucose, total cholesterol, triglycerides, blood urea, alanine aminotransferase, aspartate aminotransferase, urine sugar and urine ketone levels in diabetic rats in comparison with the diabetic control group. Additionally, this dose significantly increased body weight, hemoglobin, plasma total protein, high density lipoprotein cholesterol, liver glycogen content, superoxide dismutase, reduced glutathione and catalase levels in diabetic rats at the end of 21 days of treatment. Therefore, dietary supplementation with Boehmeria nivea root extract could be beneficial for correcting hyperglycemia, hyperlipidemia and enhancing the antioxidant defense system.

Sancheti S.,Kongju National University | Um B.-H.,Korea Institute of Science and Technology | Seo S.-Y.,Kongju National University | Seo S.-Y.,Korean Collection of Herbal Extract Inc.
South African Journal of Botany | Year: 2010

The search for a novel pharmacotherapy from medicinal plants for neurodegenerative disorders has significantly advanced. Therefore, the present study was performed to evaluate the anticholinesterase activities of one hundred medicinal plants in Korea, where Terminalia chebula (T. chebula) fruits showed significant acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitions. Further bioassay monitored phytochemical exploration led to the isolation of 1,2,3,4,6-penta-O-galloyl-β-d-glucose (compound 1), which showed significant AChE and BChE inhibitory effects with IC50 values of 29.9 ± 0.3 μM and 27.6 ± 0.2 μM, respectively. The inhibitory effect of compound 1 towards acetylcholinesterase was also evaluated using TLC and compared with tacrine as the positive control; the positive effect was confirmed. Furthermore, compound 1 also displayed strong antioxidant activity by the FRAP assay (IC50 = 4.6 ± 0.2 μM). In conclusion, compound 1 may prove to be a potential natural anti-Alzheimer source based on noteworthy AChE and BChE inhibitions, and strong antioxidant activity. © 2009 Elsevier B.V. All rights reserved.

Sancheti S.,Kongju National University | Seo S.-Y.,Kongju National University | Seo S.-Y.,Korean Collection of Herbal Extract Inc.
Experimental and Toxicologic Pathology | Year: 2013

The present study was intended to examine the effects of the supplementation of active α-glucosidase, α-amylase and lipase inhibitory ethyl acetate (CSE) fraction from the fruits of Chaenomeles sinensis (Thouin) Koehne on blood glucose (BG), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), aspartate aminotransaminase (AST), acetylcholinesterase (AChE) and antioxidant levels. The diabetic rats were treated orally with CSE at the doses of 50 and 100. mg/kg bw for 14 days. BG, TC, TG, HDL-C, ALT, AST and AChE levels were significantly reduced; on the other hand antioxidant levels were significantly increased in the treated groups. These observations suggest protective effects of CSE against STZ-induced diabetic dementia model. © 2011 Elsevier GmbH.

Sancheti S.,Kongju National University | Seo S.-Y.,Kongju National University | Seo S.-Y.,Korean Collection of Herbal Extract Inc.
Drug and Chemical Toxicology | Year: 2013

The available conventional remedies for the treatment of drug-induced liver diseases are highly inadequate and possess serious adverse effects; therefore, the development of new, effective drugs is considered necessary. This article explores the hepatoprotective and antioxidant potential of 7-methylcoumarin (MC) and 7-methoxycoumarin (MOC) in CCl4-induced hepatotoxicity in rats. MC and MOC individually, at doses of 50 and 100 mg/kg body weight, were administered orally once-daily for 7 days. The hepatoprotective activity was assessed using various biochemical parameters, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum bilirubin (TB), total protein (TP), and albumin (TA). Serum antioxidant enzyme [e.g., superoxide dismutase (SOD) and catalase (CAT)] levels were determined. Also, thiobarbituric-acid-related substances (TBARS) levels, along with histopathological studies of liver tissue, were scrutinized. Pretreatment with MC and MOC significantly decreased ALT, AST, and TB in the serum of CCl4-induced liver damaged rats in a dose-dependent manner. TA and TP levels in the serum were also restored significantly in all presupplemented MC and MOC groups. In addition, oxidative stress induced by CCl4 was prevented significantly; thereby, increasing SOD and CAT levels and decreasing TBARS levels in liver homogenates. Histopathological studies revealed the ameliorative natures of both the compounds. This study demonstrates the strong hepatoprotective activity of MC and MOC, which could be attributed to their potent antioxidant effects. © 2013 Informa Healthcare USA, Inc.

Sancheti S.,Kongju National University | Bafna M.,Kongju National University | Seo S.-Y.,Kongju National University | Seo S.-Y.,Korean Collection of Herbal Extract Inc.
Medicinal Chemistry Research | Year: 2011

Five distinct organic compounds with protected and unprotected phenolic hydroxyl groups were screened for their a-glucosidase inhibitory potential. Of these compounds, 2,4,6-trihydroxybenzaldehyde (THB) showed the strongest noncompetitive a-glucosidase inhibition (IC50 = 4.60 lM) and the most powerful antioxidant activity (DPPH assay) (IC50 = 71.4 lM) in vitro. In in vivo studies, THB significantly reduced blood glucose levels in normal and diabetic rats after glucose load compared to maltose load. © Springer Science+Business Media, LLC 2010.

Lee S.-H.,Kongju National University | Sancheti S.A.,Kongju National University | Bafna M.R.,Kongju National University | Sancheti S.S.,Kongju National University | And 2 more authors.
Journal of Medicinal Plants Research | Year: 2011

The aim of the present study was to evaluate the antiacetylcholinesterase and antioxidant potential of 80% methanolic extract of Rhododendron yedoense var. poukhanense bark (RYBE). The extract was rich in total phenolic content (160 mg gallic acid equivalent/g of dry extract) and possessed flavonoids (2.2 mg quercetin equivalent/g of dry extract). The acetylcholinesterase (AChE) inhibitory capacity of RYBE was determined against electric eel AChE enzyme. The extract exhibited substantial inhibitory activity with IC 50 value of 169.01 μg/ml. In addition, the extract revealed its strong antioxidant activity by various in vitro assay systems including 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid), ferrous ion chelating and nitric oxide scavenging assays. The reducing power of RYBE was also determined by ferric reducing antioxidant power assay. Furthermore, this extract exhibited dose dependent inhibition of lipid peroxidation in rat liver homogenate. In conclusion, due to considerable AChE inhibitory and potent antioxidant nature of RYBE, further in vivo studies along with structural elucidation to find the active ingredient(s) will prove useful in searching an effective treatment for Alzheimer's disease. © 2011 Academic Journals.

Sancheti S.,Kongju National University | Lee S.-H.,Kongju National University | Lee J.-E.,Kongju National University | Seo S.-Y.,Kongju National University | Seo S.-Y.,Korean Collection of Herbal Extract Inc.
Iranian Journal of Pharmaceutical Research | Year: 2011

Glycosidases are the enzymes involved in various biochemical processes related to metabolic disorders and diseases. Therefore, much effort has been focused on searching novel pharmacotherapy for the treatment of these ailments from medicinal plants due to higher safety margins. To pursue these efforts, the present study was performed to evaluate the et-glucosidase inhibitory activities of thirty Korean medicinal plant extracts. Among the plants studied, Euonymus sachalinensis, Rhododendron schlippenbachii, Astilbe chinensis and Juglans regia showed the strongest et-glucosidase inhibitory activity with IC50 values of 10, 20, 30 and 80 ug/ mL, respectively. In addition, Meliosma oldhamii and Symplocos chinensis showed moderate et-glucosidase inhibition with IC50 values of 150 and 220 ug/mL, respectively. Therefore, they might prove to be a potential natural source for the treatment of metabolic ailments such as, diabetes, and need further investigations. © 2011 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services.

Sancheti S.,Kongju National University | Bafna M.,Kongju National University | Seo S.-Y.,Kongju National University | Seo S.-Y.,Korean Collection of Herbal Extract Inc.
European Food Research and Technology | Year: 2010

The rising incidence of diabetes mellitus (DM) is alarming and becoming a major health problem worldwide, which is mainly associated with hyperglycemia, abnormal lipid, and antioxidant profiles. Herbal medicines are being used by about 80% of the world population primarily in the developing countries for primary health care, including DM. Based on these facts, in this study, the antihyperglycemic, antihyperlipidemic, and antioxidant properties of Chaenomeles sinensis fruits extract (CSFE) were investigated in streptozotocin (STZ)-induced (55 mg/kg body weight) diabetic rats. CSFE was found to be rich in total phenolics and flavonoid contents. The following assays were performed: fasting blood glucose, hemoglobin (Hb), blood urea nitrogen (BUN), serum total cholesterol (TC), serum triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), serum glutamic pyruvic transaminase/alanine aminotransferase (SGPT/ALT), serum glutamic oxaloacetic transaminase/aspartate aminotransaminase (SGOT/AST), liver glycogen content and superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT) contents in liver and kidney. Oral administration of CSFE (500 mg/kg body weight) significantly decreased fasting blood glucose, BUN, and serum TC, TG, LDL-C, SGPT/ALT, and SGOT/AST, while it increased blood Hb content and HDL-C in diabetic rats. Furthermore, CSFE treatment significantly increased liver glycogen content, SOD, GSH, and CAT levels in diabetic rats. The results showed that CSFE significantly inhibited the progression of diabetes induced by STZ, and the inhibitory effect of CSFE on diabetes might be associated with its hypoglycemic effect, modulation of lipid metabolism, and its ability to scavenge free radicals. © 2010 Springer-Verlag.

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