Gyeonggi do, South Korea
Gyeonggi do, South Korea

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Park S.-H.,Korea Yakult Co. | Ahn Y.-T.,Korea Yakult Co. | Huh C.-S.,Seoul National University
BMC Microbiology | Year: 2015

Background: To understand differences in the gut microbiota between elderly people of urbanized town communities (UTC) and longevity village communities (LVC), we analyzed fecal microbiota collected from individuals living in 2 UTC (Seoul and Chuncheon) and 3 LVC (Gurye, Damyang, and Soonchang) selected on the basis of indices for superlongevity (the ratio of centenarians to the total population) and longevity (the ratio of those aged 85 years or greater to those aged 65 years or greater) in South Korea by 454 pyrosequencing. Results: Taxonomy-based analysis showed that The relative abundance of Firmicutes, Tenericutes, and Actinobacteria was significantly lower in LVC than in UTC. Due to an increase of Firmicutes and a reduction of Bacteroidetes, the ratio of Firmicutes to Bacteroidetes in the gut microbiota was greater in UTC adults than in UTC children or LVC adults. The population levels of Bacteroides, Prevotella, and Lachnospira were significantly higher in LVC than in UTC, but the levels of Dialister, Subdoligranulum, Megamonas, EF401882-g, and AM275436-g were lower in LVC than in UTC. Although most of the species detected in LVC were detected in UTC, some Bacteroides spp. and Faecalibacterium spp. were detected only in LVC. Among Bacteroides spp., ACWH-s, EF403317-s, and EF403722-s were detected in children and LVC samples only but FJ363527-s, 4P000677-s, and 4P000015-s were detected in UTC samples. EF402172-s and EF404388-s, members of Faecalibacterium spp., which are known to have anti-inflammatory properties, were detected in LVC and children only (>3.9% of total sequence). In addition, the fecal lipopolysaccharides (LPS) content was significantly higher in UTC than in LVC. Conclusions: These findings suggest that maintaining gut microbiota, including Faecalibacterium spp. EF402172-s and EF404388-s, as well as low LPS levels may play an important role in preserving residents' health in LVC. © 2015 Park et al.; licensee BioMed Central.

Park D.-Y.,Korea Yakult Co. | Ahn Y.-T.,Korea Yakult Co. | Huh C.-S.,Korea Yakult Co. | Mcgregor R.A.,Kyungpook National University | Choi M.-S.,Kyungpook National University
World Journal of Gastroenterology | Year: 2013

AIM: To investigate the effect of novel probiotics on the clinical characteristics of high-fructose induced metabolic syndrome. METHODS: Male Wistar rats aged 4 wk were fed a 70% w/w high-fructose diet (n = 27) or chow diet (n = 9) for 3 wk to induce metabolic syndrome, the rats were then randomized into groups and administered probiotic [Lactobacillus curvatus (L. curvatus) HY7601 and Lactobacillus plantarum (L. plantarum) KY1032] at 109 cfu/d or 1010 cfu/d or placebo by oral gavage for 3 wk. Food intake and body weight were measured once a week. After 6 wk, the rats were fasted for 12 h, then anesthetized with diethyl ether and sacrificed. Blood samples were taken from the inferior vena cava for plasma analysis of glucose, insulin, C-peptide, totalcholesterol, triglycerides and thiobarbituric acid-reacting substances. Real-time polymerase chain reaction was performed using mouse-specific Taqman probe sets to assess genes related to fatty acid β-oxidation, lipogenesis and cholesterol metabolism in the liver. Target gene expression was normalized to the housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase. RESULTS: Rodents fed a high-fructose diet developed clinical characteristics of the metabolic syndrome including increased plasma glucose, insulin, triglycerides, total cholesterol and oxidative stress levels, as well as increased liver mass and liver lipids compared to chow fed controls. Probiotic treatment (L. curvatus HY7601 and L. plantarum KY1032) at high (1010 cfu/d) or low dosage (109 cfu/d) lowered plasma glucose, insulin, triglycerides and oxidative stress levels. Only high-dose probiotic treatment reduced liver mass and liver cholesterol. Probiotic treatment reduced lipogenesis via downregulation of SREBP1, FAS and SCD1 mRNA levels and increased β-oxidation via up-regulation of PPARα and CPT2 mRNA levels. CONCLUSION: Probiotic L. curvatus HY7601 and L. plantarum KY1032 combined suppressed the clinical characteristics of high-fructose-induced metabolic syndrome, therefore, may provide a natural alternative for the treatment of diet-induced metabolic syndrome. © 2013 Baishideng. All rights reserved.

Yoo S.-R.,Kyungpook National University | Kim Y.-J.,Kyungpook National University | Park D.-Y.,Seoul National University | Jung U.-J.,Kyungpook National University | And 5 more authors.
Obesity | Year: 2013

Objective: To determine the effects of naturally derived probiotic strains individually or combination on a short-term diet-induced obesity model. Design and Methods: C57BL/6J mice (n = 50) were randomly divided into five groups, then fed a high-fat high-cholesterol diet (HFCD), HFCD and Lactobacillus plantarum KY1032 (PL, 1010cfu/day), HFCD and Lactobacillus curvatus HY7601 (CU, 1010cfu/day), HFCD and in combination with PL+CU (10 10cfu/day), or a normal diet (ND) for 9 weeks. Results: PL and CU showed distinct and shared metabolic activity against a panel of 50 carbohydrates. Fat accumulation in adipose tissue and liver was significantly reduced by probiotic strains CU or PL+CU. Probiotic strains CU or PL+CU reduced cholesterol in plasma and liver, while PL+CL had a synergistic effect on hepatic triglycerides. Probiotic strains PL+CU combination was more effective for inhibiting gene expressions of various fatty acid synthesis enzymes in the liver, concomitant with decreases in fatty acid oxidation-related enzyme activities and their gene expressions. Conclusions: Multi-strain probiotics may prove more beneficial than single-strain probiotics to combat fat accumulation and metabolic alterations in diet-induced obesity. Copyright © 2013 The Obesity Society.

Park D.-Y.,Korea Yakult Co | Ahn Y.-T.,Korea Yakult Co | Huh C.-S.,Korea Yakult Co | Jeon S.-M.,Kyungpook National University | Choi M.-S.,Kyungpook National University
Journal of Medicinal Food | Year: 2011

Some probiotics and their cell components are known to modulate lipid metabolism in vitro and/or in vivo. This study was carried out to investigate possible anti-adipogenic action of a probiotic cell extract, Lactobacillus plantarum KY1032 cell extract (KY1032-CE), in vitro using 3T3-L1 cells. Lipid regulation in the cell culture system was assessed by AdipoRed assay and Oil red O staining of intracellular lipids and real-time polymerase chain reaction and western blot analysis of adipogenesis-related factors. AdipoRed assay revealed that KY1032-CE treatment significantly decreased lipid accumulation in maturing 3T3-L1 preadipocytes in a dose-dependent manner. Oil red O staining demonstrated that KY1032-CE reduced the number of lipid-containing rounded cells. KY1032-CE down-regulated the mRNA and protein expression of four adipocyte-specific genes: peroxisome proliferator-activated receptor-γ2, CCAAT/enhancer binding protein-α, fatty acid synthase, and adipocyte-fatty acid binding protein. Accordingly, these results indicate that KY1032-CE can reduce fat mass by modulating adipogenesis in maturing preadipocytes. Further studies are needed to elucidate its mode of actions in efficacy tests of KY1032-CE in vivo. © Copyright 2011, Mary Ann Liebert, Inc.

Ahn H.Y.,Yonsei University | Kim M.,Yonsei University | Chae J.S.,Yonsei University | Ahn Y.-T.,Korea Yakult Co. | And 4 more authors.
Atherosclerosis | Year: 2015

Objective: Previous studies have indicated that supplementation with probiotics might improve lipid metabolism. The objective of the study was to evaluate the effect of supplementation with probiotic strains Lactobacillus curvatus (L.curvatus) HY7601 and Lactobacillus plantarum (L.plantarum) KY1032 on triglyceride (TG) and apolipoprotein A-V (apo A-V) levels. Methods: A randomized, double-blinded, placebo-controlled study was conducted with 128 non-diabetic subjects with hypertriglyceridemia. Over a 12-week test period, the probiotic group consumed 2g/day of a powdered supplement containing L.curvatus HY7601 and L.plantarum KY1032, whereas the placebo group consumed a powder lacking probiotics. Results: After the treatment, the probiotic group showed an 18.3% (P<0.001) reduction in TGs and increases of 21.1% (P=0.001) and 15.6% (P<0.001) in the apo A-V and LDL particle size, respectively. The probiotic group had a significant reduction in TGs (P=0.040) and increases in the plasma apo A-V (P=0.003) and LDL particle size (P<0.001) compared with the placebo group. In the probiotic group, the reduction in the TG levels was negatively correlated with changes in the apo A-V and baseline TGs, regardless of the APOA5 -1131T>C genotype. Conclusion: The consumption of two probiotic strains for 12 weeks reduced TGs and increased the apo A-V and LDL particle size in hypertriglyceridemic subjects. This effect was more pronounced in subjects with higher levels of fasting TGs regardless of their APOA5 -1131T>C genotype. © 2015 Elsevier Ireland Ltd.

Paldo Co. and Korea Yakult Co. | Date: 2011-11-16

uncooked noodles; instant noodles; noodles; spaghetti; uncooked Chinese noodles; udon noodles; instant soba noodles; instant udon noodles; instant Chinese noodles; vermicelli.

Paldo Co. and Korea Yakult Co. | Date: 2011-11-22

uncooked noodles; instant noodles; noodles; spaghetti; uncooked Chinese noodles; udon noodles; instant soba noodles; instant udon noodles; instant Chinese noodles; vermicelli.

Paldo Co. and Korea Yakult Co. | Date: 2010-02-09

Extracts of hops for making beer; fruit juices, namely, grape juices, pineapple juices, apple juices, and orange juices; energy drinks; beverages containing ginseng extracts; sherbets beverages; syrups for making fruit drinks; orange juices beverages; soda water; soda pop; apple juice beverages; non-alcoholic beverages, namely, carbonated beverages and malt beverages; non-alcoholic fruit extracts used in the preparation of beverages; non-alcoholic fruit juice beverages, lemon squash, lemonades, fruit juices, tomato juices, vegetable juice beverages, mineral water beverages, beer.

Paldo Co. and Korea Yakult Co. | Date: 2010-04-13

Ramyun, namely, instant noodles.

Kim K.-A.,Kyung Hee University | Jung I.-H.,Kyung Hee University | Park S.-H.,Korea Yakult Co. | Ahn Y.-T.,Korea Yakult Co. | And 2 more authors.
PLoS ONE | Year: 2013

Panax ginseng (family Araliaceae) which contains ginsenoside Rb1 as a main constituent is traditionally used as a remedy for cancer, inflammation, stress, and ageing. The ginsenoside Rb1 in orally administered ginseng is metabolized to bioactive compounds by gut microbiota before their absorptions to the blood. However, its metabolizing activities in individuals are significantly different as we previously demonstrated. Here, we selected 5 samples with fecal activity potently metabolizing ginsenoside Rb1 to compound K (FPG; metabolic activity, 0.058±0.029 pmol/min/mg) and 5 samples with fecal activity non-metabolizing ginsenoside Rb1 to compound K (FNG) from a pool of 100 subjects investigated in a previous study and analyzed fecal microbiota by 16S rRNA gene pyrosequencing. Taxonomy-based analysis showed that the population levels of Firmicutes and Proteobacteria in FPG were lower than in FNG, but those of Bacteroidetes and Tenericutes in FPG were higher than in FNG. At the genus level, the population levels of Clostridiales_uc_g, Oscillibacter, Ruminococcus, Holdemania, and Sutterella in FPG were significantly higher than in FNG, but that of Leuconostoc in FPG was lower than in FNG. The population levels of Bacteroides and Bifidobacterium, which potently metabolizes ginsenoside Rb1 to compound K were dramatically increased in FPG. The gut microbiota compositions of FPG and FNG were segregated on PCO2 by Principal Coordinate Analysis. Intestinal bacterial metabolism of ginseng, particularly ginsenoside Rb1, may be dependent on the composition of gut microbiota, such as Ruminococcus spp., Bacteroides spp. and Bifidobacterium spp. © 2013 Kim et al.

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