Kim H.-W.,Korea UniversitySeoul |
Kim C.-Y.,Korea UniversitySeoul |
Blake R.,Vanderbilt University
Current Biology | Year: 2017
Early visual experience sculpts neural mechanisms that regulate the balance of influence exerted by the two eyes on cortical mechanisms underlying binocular vision [1, 2], and experience's impact on this neural balancing act continues into adulthood [3–5]. One recently described, compelling example of adult neural plasticity is the effect of patching one eye for a relatively short period of time: contrary to intuition, monocular visual deprivation actually improves the deprived eye's competitive advantage during a subsequent period of binocular rivalry [6–8], the robust form of visual competition prompted by dissimilar stimulation of the two eyes [9, 10]. Neural concomitants of this improvement in monocular dominance are reflected in measurements of brain responsiveness following eye patching [11, 12]. Here we report that patching an eye is unnecessary for producing this paradoxical deprivation effect: interocular suppression of an ordinarily visible stimulus being viewed by one eye is sufficient to produce shifts in subsequent predominance of that eye to an extent comparable to that produced by patching the eye. Moreover, this imbalance in eye dominance can also be induced by prior, extended viewing of two monocular images differing only in contrast. Regardless of how shifts in eye dominance are induced, the effect decays once the two eyes view stimuli equal in strength. These novel findings implicate the operation of interocular neural gain control that dynamically adjusts the relative balance of activity between the two eyes [13, 14]. © 2017 Elsevier Ltd
Song M.,Korea UniversitySeoul |
Jo Y.S.,Korea UniversitySeoul |
Lee Y.-K.,Korea UniversitySeoul |
Choi J.-S.,Korea UniversitySeoul
Behavioural Brain Research | Year: 2017
The lateral habenula (LHb) is an epithalamic brain structure that provides strong projections to midbrain monoaminergic systems that are involved in motivation, emotion, and reinforcement learning. LHb neurons are known to convey information about aversive outcomes and negative prediction errors, suggesting a role in learning from aversive events. To test this idea, we examined the effects of electrolytic lesions of the LHb on signaled two-way active avoidance learning in which rats were trained to avoid an unconditioned stimulus (US) by taking a proactive shuttling response to an auditory conditioned stimulus (CS). The lesioned animals learned the avoidance response significantly faster than the control groups. In a separate experiment, we also investigated whether the LHb contributes to Pavlovian threat (fear) conditioning and extinction. Following paired presentations of the CS and the US, LHb-lesioned animals showed normal acquisition of conditioned response (CR) measured with freezing. However, extinction of the CR in the subsequent CS-only session was significantly faster. The enhanced performance in avoidance learning and in threat extinction jointly suggests that the LHb normally plays an inhibitory role in learning driven by absence of aversive outcomes. © 2016 Elsevier B.V.
Bok H.H.,Pohang University of Science and Technology |
Bok H.H.,POSCO |
Choi J.W.,POSCO |
Suh D.W.,Pohang University of Science and Technology |
And 2 more authors.
International Journal of Plasticity | Year: 2015
Abstract Elastically driven shape change, or springback, in a press-hardened U-channel part made from a tailor-welded blank (TWB) was simulated using a fully coupled thermo-mechanical-metallurgical finite element (FE) method. The TWB consists of boron steel and high-strength low-alloy steel, which have significantly different hardenabilities. A combined implicit-explicit three-step simulation consisting of air cooling, forming and die quenching, and springback was used for computational efficiency. All the required material models such as the modified phase-transformation kinetics and phase-transformation-related stress-update scheme were implemented in the FE software ABAQUS with the user-defined subroutines UMAT, VUMAT, and HETVAL. The developed FE procedure, including the material models, satisfactorily predicted the experimentally measured shape changes of the TWB part. Here we present an in-depth analysis of the residual stress development during forming and die quenching using different material modeling schemes. It should be noted that the stress evolution of the two materials with high and low hardenabilities were significantly different depending on the phase transformation kinetics during forming and quenching. Moreover, in order to enhance the prediction capability of the press-hardening simulations, it was essential to include the phase-transformation-related strains in the material model. © 2014 Elsevier Ltd.
Li Y.-X.,Korea UniversitySeoul |
Lim S.-T.,Korea UniversitySeoul
Carbohydrate Polymers | Year: 2016
Aqueous dispersions prepared with OSA-modified high amylose starch were investigated in comparison with native high amylose starch and beta-cyclodextrin using alpha-lipoic acid as a model substance. Alpha-lipoic acid (ALA), a lipophilic antioxidant essential for energy metabolism in human, was dispersed in gelatinized starch solutions (1.0% w/v) at different temperatures (50-90 °C) and times (3-12 h). High amylose starch modified with 3% OSA (dry starch base) was most favored in maximizing the dispersibility of ALA (84% recovery) under mild heating (70 °C for 3 h). The optimally prepared dispersion was milky white and contained particles with a narrow size distribution (200-300 nm). The precipitate isolated from the dispersion contained crystalline V-complexes of ALA and amylose while the supernatant contained free ALA accounting for 1/3 of total ALA, indicating OSA-modified high amylose starch stabilized ALA either by complexing with amylose or by retarding aggregation of ALA. © 2015 Elsevier Ltd. All rights reserved.
Lee H.-J.,Korea UniversitySeoul |
Kim S.-H.,Korea UniversitySeoul |
Lee J.-C.,Korea UniversitySeoul
Scripta Materialia | Year: 2016
Despite its excellent strengthening abilities, incorporation of C into most non-ferrous metals is limited by its solubility. In this study, C diffusion in pure Ti powders was promoted in a quantifiable amount using a combined technique of spark plasma sintering and powder metallurgy to synthesize a bulk Ti alloy. The sintered Ti was free from elements toxic to the human body and showed excellent strength, ductility, and wear resistance, which well exceeded those of the commercial Ti-6Al-4V alloy counterpart. Here, we report on the physics underlying the alloy design, its synthesis, resultant properties, and possible applications. © 2015 Elsevier B.V.
Cho Y.-H.,Korea UniversitySeoul |
Yoo S.-D.,Korea UniversitySeoul
Frontiers in Plant Science | Year: 2015
The signaling of the plant hormone ethylene has been studied genetically, resulting in the identification of signaling components from membrane receptors to nuclear effectors. Among constituents of the hormone signaling pathway, functional links involving a putative mitogen-activated protein kinase kinase CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) and a membrane transporter-like protein ETHYLENE INSENSITIVE2 (EIN2) have been missing for a long time. We now learn that EIN2 is cleaved and its C-terminal end moves to the nucleus upon ethylene perception at the membrane receptors, and then the C-terminal end of EIN2 in the nucleus supports EIN3-dependent ethylene-response gene expression. CTR1 kinase activity negatively controls the EIN2 cleavage process through direct phosphorylation. Despite the novel connection of CTR1 with EIN2 that explains a large portion of the missing links in ethylene signaling, our understanding still remains far from its completion. This focused review will summarize recent advances in the EIN3-dependent ethylene signaling mechanisms including CTR1-EIN2 functions with respect to EIN3 regulation and ethylene responses. This will also present several emerging issues that need to be addressed for the comprehensive understanding of signaling pathways of the invaluable plant hormone ethylene. © 2015 Cho and Yoo.
Lee M.H.,University of Texas at Austin |
Kim J.S.,Korea UniversitySeoul |
Sessler J.L.,University of Texas at Austin
Chemical Society Reviews | Year: 2015
Quantitative determination of specific analytes is essential for a variety of applications ranging from life sciences to environmental monitoring. Optical sensing allows non-invasive measurements within biological milieus, parallel monitoring of multiple samples, and less invasive imaging. Among the optical sensing methods currently being explored, ratiometric fluorescence sensing has received particular attention as a technique with the potential to provide precise and quantitative analyses. Among its advantages are high sensitivity and inherent reliability, which reflect the self-calibration provided by monitoring two (or more) emissions. A wide variety of ratiometric sensing probes using small fluorescent molecules have been developed for sensing, imaging, and biomedical applications. In this research highlight, we provide an overview of the design principles underlying small fluorescent probes that have been applied to the ratiometric detection of various analytes, including cations, anions, and biomolecules in solution and in biological samples. This highlight is designed to be illustrative, not comprehensive. This journal is © The Royal Society of Chemistry.
Lee M.H.,Sookmyung Womens University |
Sessler J.L.,University of Texas at Austin |
Kim J.S.,Korea UniversitySeoul
Accounts of Chemical Research | Year: 2015
Conspectus Theranostics, chemical entities designed to combine therapeutic effects and imaging capability within one molecular system, have received considerable attention in recent years. Much of this interest reflects the promise inherent in personalized medicine, including disease-targeted treatments for cancer patients. One important approach to realizing this latter promise involves the development of so-called theranostic conjugates, multicomponent constructs that selectively target cancer cells and deliver cytotoxic agents while producing a readily detectable signal that can be monitored both in vitro and in vivo. This requires the synthesis of relatively complex systems comprising imaging reporters, masked chemotherapeutic drugs, cleavable linkers, and cancer targeting ligands. Ideally, the cleavage process should take place within or near cancer cells and be activated by cellular components that are associated with cancer states or specifically expressed at a higher level in cancer cells. Among the cleavable linkers currently being explored for the construction of such localizing conjugates, disulfide bonds are particularly attractive. This is because disulfide bonds are stable in most blood pools but are efficiently cleaved by cellular thiols, including glutathione (GSH) and thioredoxin (Trx), which are generally found at elevated levels in tumors. When disulfide bonds are linked to fluorophores, changes in emission intensity or shifts in the emission maxima are typically seen upon cleavage as the result of perturbations to internal charge transfer (ICT) processes. In well-designed systems, this allows for facile imaging. In this Account, we summarize our recent studies involving disulfide-based fluorescent drug delivery conjugates, including preliminary tests of their biological utility in vitro and in vivo.To date, a variety of chemotherapeutic agents, such as doxorubicin, gemcitabine, and camptothecin, have been used to create disulfide-based conjugates, as have a number of fluorophores, including naphthalimide, coumarin, BODIPY, rhodol, and Cy7. The resulting theranostic core (drug-disulfide-fluorophore) can be further linked to any of several site-localizing entities, including galactose, folate, biotin, and the RGD (Arg-Gly-Asp) peptide sequence, to create systems with an intrinsic selectivity for cancer cells over normal cells. Site-specific cleavage by endogenous thiols serves to release the cytotoxic drug and produce an easy-to-monitor change in the fluorescence signature of the cell. On the basis of the results summarized in this Account, we propose that disulfide-based cancer-targeting theranostics may have a role to play in advancing drug discovery efforts, as well as improving our understanding of cellular uptake and drug release mechanisms. © 2015 American Chemical Society.
Li Y.,Xi'an Jiaotong University |
Kim J.,Korea UniversitySeoul
Pattern Recognition | Year: 2015
We describe a fast and efficient numerical algorithm for the process of three-dimensional narrow volume reconstruction from scattered data in three dimensions. The present study is an extension of previous research [Li et al., Surface embedding narrow volume reconstruction from unorganized points, Comput. Vis. Image Underst. 121 (2014) 100-107]. In the previous work, we modified the original Allen-Cahn equation by multiplying a control function to restrict the evolution within a narrow band around the given surface data set. The key idea of the present work is to perform the computations only on a narrow band around the given surface data set. In this way, we can significantly reduce the storage memory and CPU time. The proposed numerical method, based on operator splitting techniques, can employ a large time step size and exhibits unconditional stability. We perform a number of numerical experiments in order to demonstrate the efficiency of this method. © 2015 Elsevier Ltd.
Woo K.Y.,Korea UniversitySeoul |
Kim K.H.,Korea UniversitySeoul |
Kim T.G.,Korea UniversitySeoul
IEEE Photonics Technology Letters | Year: 2016
We fabricated Ga2O3-based transparent conductive electrodes (TCEs) for use in near-ultraviolet (NUV) lightemitting diodes (LEDs) by embedding metal layers into Ga2O3. We employ Ni and Ag layers to improve current injection and spreading properties. Our fabricated Ni/Ga2O3/Ag/Ga2O3 multilayer (annealed at 600 °C, 1 min) deposited on an NUV LED wafer exhibits 83% transmittance at 385 nm with a specific contact resistance of 8 × 10-3 Ω • cm2. An NUV LED fabricated with a Ni/Ga2O3/Ag/Ga2O3 TCE exhibits a 17% increase in light output power at 150 mA and a 3.2% decrease in forward voltage at 20 mA over those of an indium tin oxide NUV LED. © 2015 IEEE.