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Suigen, South Korea

Kwon Y.S.,Korea Seed and Variety Service
Seed Science and Technology | Year: 2013

EST-SSR markers were used to identify 36 candidate and registration watermelon varieties and 20 major commercial watermelon varieties, and to assess hybrid seed purity of a commercial hybrid watermelon variety known as 'Speedggul'. A set of 300 EST-SSR primer pairs were applied for fingerprinting the watermelon varieties. Nine markers showed hyper-variability and were able to differentiate 56 genotypes, representing the genetic variation within varietal groups. Cluster analysis and Jaccard's distance coefficients using UPGMA (Unweighted Pair Group Method with Arithmetic Mean) categorised varieties into two major groups corresponding to morphological traits. To evaluate the use of EST-SSR marker analysis for seed purity testing of F1 hybrid watermelon Speedggul, the results of marker analysis were compared with the results of a grow-out test. The F1 purity through grow-out test was in accordance with molecular marker analysis. These markers will be useful for fingerprinting watermelon varieties and help private seed companies to improve watermelon seed purity. Source


Hong J.-H.,Korea Seed and Variety Service | Choi K.-J.,National Institute of Horticultural and Herbal Science | Kwon Y.-S.,Dong - A University
Korean Journal of Horticultural Science and Technology | Year: 2014

This study was carried out to construct a DNA profile database of 100 strawberry cultivars using microsatellite markers. Two hundred seventy four microsatellite primer pairs were screened with a set of 21 strawberry cultivars with different morphological traits. Twenty five primer pairs were selected because they produced reliable and reproducible fingerprints. These primer pairs were used to develop DNA profiles of 100 strawberry cultivars. Three to thirteen alleles were detected by each marker with an average of 7.50. The average polymorphism information content varied from 0.331 to 841 (average 0.706). Cluster analysis showed that the 100 cultivars were divided into 7 major groups reflecting geographic origin and pedigree information. Moreover, most of the cultivars could be discriminated by marker genotypes. These markers will be useful as a tool for the protection of plant breeders’ intellectual property rights in addition to providing the means to intervene seed disputes relating to variety authentication. © 2014 Korean Society for Horticultural Science. Source


Kwon Y.-S.,Dong - A University | Hong J.-H.,Korea Seed and Variety Service
Korean Journal of Horticultural Science and Technology | Year: 2014

Microsatellite markers were used to identify 58 major commercial melon cultivars, and to assess hybrid seed purity of a melon breeding line known as ‘10H08’. A set of 412 microsatellite primer pairs were utilized for fingerprinting of the melon cultivars. Twenty-nine markers showed hyper-variability and could discriminate all cultivars on the basis of marker genotypes, representing the genetic variation within varietal groups. Cluster analysis based on Jaccard’s distance coefficients using the UPGMA algorithm categorized 2 major groups, which were in accordance to morphological traits. The DNA bulks of female and male parents of breeding line ‘10H08’ were tested with 29 primer pairs based on microsatellites to investigate purity testing of F1 hybrid seeds, and 5 primer pairs exhibited polymorphism. One microsatellite primer pair (CMGAN12) produced unambiguous polymorphic bands among the parents. Among 192 seeds tested with CMGAN12, progeny possibly generated by self-pollination of the female parent were clearly distinguished from the hybrid progeny. These markers will be useful for fingerprinting melon cultivars and can help private seed companies to improve melon seed purity. Ⓒ 2014 Korean Society for Horticultural Science. Source


Kwon Y.-S.,Korea Seed and Variety Service | Choi K.-J.,Korea Seed and Variety Service
Korean Journal of Horticultural Science and Technology | Year: 2013

Microsatellite is one of the most suitable marker for cultivar identification as it has great discrimination power for cultivars with narrow genetic variation. The polymorphism level between 358 microsatellite primer pairs and 11 commercial cucumber cultivars was investigated. Thirty-one primer pairs showed high polymorphism within cucumber cultivars with different fruit types. These markers were applied for the constructing DNA profile data base of 110 commercial cucumber cultivars through multiplex PCR and fluorescence based automatic detection system. A total of 139 polymorphic amplified fragments were obtained by using 31 microsatellite markers. The average of PIC value was 0.610 ranging from 0.253 to 0.873. One hundred and thirty nine microsatellite loci were used to calculate Jaccard's distance coefficients for UPGMA cluster analysis. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into plant shape and fruit type. Almost the cultivars were discriminated by marker genotypes. This information may be useful to compare through genetic relationship analysis between existing variety and candidate varieties in distinctive tests and protection of plant breeders' intellectual property rights through variety identification. Source


Kwon Y.-S.,Korea Seed and Variety Service | Hong J.-H.,Korea Seed and Variety Service | Choi K.-J.,Korea Seed and Variety Service
Korean Journal of Horticultural Science and Technology | Year: 2013

This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test. © 2013 Korean Society for Horticultural Science. Source

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