Kim H.-H.,Kyungpook National University |
Kim H.-H.,Korea Promotion Institute for Traditional Medicine Industry |
Bae Y.,Kyungpook National University |
Kim S.-H.,Kyungpook National University
Food and Chemical Toxicology | Year: 2013
A great number of people are suffering from allergic inflammatory disease such as asthma, atopic dermatitis, and sinusitis. Therefore discovery of drugs for the treatment of these diseases is an important subject in human health. In this study, we investigated anti-allergic inflammatory effect of galangin and underlying mechanisms of action using in vitro and in vivo models. Galangin inhibited histamine release by the reduction of intracellular calcium in phorbol 12-mystate 13-acetate plus calcium ionophore A23187-stimulated human mast cells (HMC-1). Galangin decreased expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and IL-8. The inhibitory effect of galangin on theses pro-inflammatory cytokines was related with c-Jun N-terminal kinases, and p38 mitogen-activated protein kinase, nuclear factor-κB, and caspase-1. Furthermore, galangin attenuated IgE-mediated passive cutaneous anaphylaxis and the expression of histamine receptor 1 at the inflamed tissue. The inhibitory effects of galangin were more potent than cromolyn, a known anti-allergic drug. Our results showed that galangin down-regulates mast cell-derived allergic inflammatory reactions by blocking histamine release and expression of pro-inflammatory cytokines. In light of in vitro and in vivo anti-allergic inflammatory effects, galangin could be a beneficial anti-allergic inflammatory agent. © 2013 Elsevier Ltd.
Anti-inflammatory Potential of Quercetin-3-O-β-D-("2"-galloyl)-glucopyranoside and Quercetin Isolated from Diospyros kaki calyx via Suppression of MAP Signaling Molecules in LPS-induced RAW 2647 Macrophages
PubMed | Korea University, Advanced Medical Fusion Textile Center, Herbnoori Co., Wonkwang University and 3 more.
Type: Journal Article | Journal: Journal of food science | Year: 2016
Diospyros kaki (DK) contains an abundance of flavonoids and has been used in folk medicine in Korea for centuries. Here, we report for the first time the anti-inflammatory activities of Quercetin (QCT) and Quercetin 3-O--(2-galloyl)-glucopyranoside (Q32G) isolated from DK. We have determine the no cytotoxicity of Q32G and QCT against RAW 264.7 cells up to concentration of 50 M. QCT and Q32G demonstrated potent anti-inflammatory activities by reducing expression of nitric oxide (NO), tumor necrosis factor (TNF)-, interleukin (IL)-1, IL-6 inducible NO synthase (iNOS), cyclooxygenase (COX)-2, and mitogen-activated protein kinase (MAPKs) in mouse RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Both QCT or Q32G could decrease cellular protein levels of COX-2 and iNOS as well as secreted protein levels of NO, PGE
Kim Y.A.,Korea Promotion Institute for Traditional Medicine Industry |
Kong C.-S.,Silla University |
Lee J.I.,Korea University |
Kim H.,Korea University |
And 4 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2012
As a part of an ongoing search for novel antioxidants from the salt marsh plants, bioactivity-isolation and structure determination of constituents from Salicornia herbacea were performed. One new triterpenoid saponin (4), along with three known saponins (1-3), has been isolated from n-BuOH fraction of S. herbacea. On the basis of the spectroscopic methods, the structure of the new saponin 4 was elucidated as 3β-hydroxy-23-oxo-30-noroleana-12, 20(29)-diene-28-oic acid 3-O-β-d-glucuronopyranosyl-28-O-β-d- glucopyranoside. Scavenging effects of saponins 1-4 were examined on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical and peroxynitrite. Particularly, saponin 3 exerted significant antioxidant activity on both authentic peroxynitrite and peroxynitrite generated from morpholinosydnonimine (SIN-1). © 2012 Elsevier Ltd. All rights reserved.
Cho S.J.,Gyeongnam National University of Science and Technology |
Kwon H.S.,Korea Promotion Institute for Traditional Medicine Industry
Journal of Applied Biological Chemistry | Year: 2015
Five phenylpropanoids (1-5), a benzofuran neolignan (6), two 8-O-4′-neolignans (7-8), and five tetrahydrofuran lignans (9-13) were isolated from a methanol extract of Myristica fragrans seeds. The structures of 1-13 were determined by1Hand13C-NMR spectroscopic data analyses and a comparison with the literature data. Compound 3 was isolated for the first time from this plant. All the isolated compounds were evaluated for their inhibitory activity against tyrosinase. Among them, safrole (1) showed significant inhibitions against both the monophenolase (IC50=32.11 μM) and diphenolase (IC50=27.32 μM) activities of tyrosinase. The kinetic analysis shows that safrole (1) is competitive inhibitors for both monophenolase and diphenolase. The apparent inhibition constant (Ki) for safrole (1) binding with free enzyme was determined to be 16.05 and 13.66 μM for monophenolase and diphenolase, respectively. © The Korean Society for Applied Biological Chemistry 2015.
Nagajyothi P.C.,Dongguk University |
Cha S.J.,Daegu Haany University |
Yang I.J.,Dongguk University |
Sreekanth T.V.M.,Catholic University of Daegu |
And 3 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2015
The exploitation of various plant materials for the green synthesis of nanoparticles is considered an eco-friendly technology because it does not involve toxic chemicals. In this study, zinc oxide nanoparticles (ZnO NPs) were synthesized using the root extract of Polygala tenuifolia. Synthesized ZnO NPs were characterized by UV-Vis spectroscopy, FTIR, TGA, TEM, SEM and EDX. Anti-inflammatory activity was investigated in LPS-stimulated RAW 264.7 macrophages, whereas antioxidant activity was examined using a DPPH free radical assay. ZnO NPs demonstrated moderate antioxidant activity by scavenging 45.47% DPPH at 1 mg/mL and revealed excellent anti-inflammatory activity by dose-dependently suppressing both mRNA and protein expressions of iNOS, COX-2, IL-1β, IL-6 and TNF-α. © 2015 Elsevier B.V. All rights reserved.
Park H.-H.,Korea Promotion Institute for Traditional Medicine Industry |
Park N.-Y.,Korea Promotion Institute for Traditional Medicine Industry |
Kim S.-G.,Korea Promotion Institute for Traditional Medicine Industry |
Jeong K.-T.,Korea Promotion Institute for Traditional Medicine Industry |
And 2 more authors.
American Journal of Chinese Medicine | Year: 2015
Wound healing is a complex process orchestrated by the regeneration of the epithelium and the remodeling of the extracellular matrix through processes like collagen deposition. Galla Rhois has been widely used in traditional Korean medicine for its various pharmacological effects, including an anticoccidial effect, however, little is known about its healing activity. The purpose of this study was to determine the effects of Galla Rhois ethanol extract (GRE) on wound healing activities, including H2O2-induced oxidative stress, cell migration, and lactate dehydrogenase (LDH) release assays using human keratinocyte (HaCaT) and dermal fibroblasts (CCD-986SK). In addition, total soluble collagen deposition and collagen gene expression for Type I and III collagen were evaluated in CCD-986SK. Total tannin and flavonoid contents for GRE were measured. GRE induced a significant increase in the number and migration of cells, along with a decrease in cell death and LDH release. In addition, it also induced the over-expression of collagen Type I and III mRNA and caused increased synthesis of total soluble collagen. The contents of total tannin and flavonoid for GRE were 55.7% (556.99±2.60mg/g) and 62.9% (62.89±1.35mg/g), respectively. The results suggest that GRE can cause accelerated wound healing by increasing cell survival, proliferation, migration, and collagen synthesis along with a potential anti-oxidant property. This evidence provides novel insight into natural therapy for tissue injury. © 2015 World Scientific Publishing Company.
Kim H.H.,Chung - Ang University |
Kim D.H.,Korea Promotion Institute for Traditional Medicine Industry |
Kim M.H.,Chung - Ang University |
Oh M.H.,Chung - Ang University |
And 3 more authors.
Archives of Pharmacal Research | Year: 2013
The leaves of Myrica rubra sieb. et zucc. have been used in oriental traditional medicine for the treatment of burns, skin diseases, and as an antidiarrheal in China, Japan, and Korea. Activity guided isolation of the leaves of M. rubra has led to the isolation of five flavonoid: myricetin (1), myricitrin (2), myricetin 3-O-(2″-O-galloyl)-α-L-rhamnopyranoside (3), myricetin 3-O-(2″-O-galloyl)-β-D-galactopyranoside (4), and quercetin 3-O-(2″-O-galloyl)-β-D-galactopyranoside (5). All isolates were evaluated for their antioxidant potency against the superoxide anion (O2 -), and compounds 3-5 showed potent scavenging activities with 50 % inhibition concentration (IC50) values compared to the positive control, allopurinol. Compounds 1-5 were evaluated as inhibitors of various macrophage functions involved in the inflammatory process. These five compounds significantly and dose dependently inhibited lipopolysaccharide (LPS)-stimulated nitric oxide (NO), pro-inflammatory cytokines, and the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophages. Our results suggest that galloyl flavonol glycosides (3-5) isolated from M. rubra might be beneficial for the treatment of inflammationa-related diseases. © 2013 The Pharmaceutical Society of Korea.
Kim J.-O.,Korea Promotion Institute for Traditional Medicine Industry |
Lee G.-D.,Joongbu University
Food Science and Biotechnology | Year: 2013
Extraction conditions of the fruit of cheonnyuncho (Opuntia ficus-indica) were optimized by using response surface methodology. Accordingly, the extract yield of the cheonnyuncho fruit was maximum when extracted at 85. 69°C for 92. 89 min using amount of water to sample of 23. 46 mL/g. Moreover, the optimum conditions for the maximum yield of effective substances were as follows: flavonoid 89. 80°C, 170. 81 min, and 17. 07 mL/g; phenolics 98. 30°C, 96. 46 min, and 15. 93 mL/g; electron donating ability 71. 00°C, 173. 58min, and 15. 22mL/g; reducing sugars 69. 87°C, 68. 84 min, and 16. 29 mL/g; pectins 96. 54°C, 135. 28 min, and 20. 02 mL/g; and vitamin C 61. 80°C, 101. 98 min, and 12. 14 mL/g, respectively. The optimum extraction condition ranges for obtaining desirable physiochemical properties of cheonnyuncho extracts were 85-100°C for 60-120 min using 18-25 mL/g of amount of water to sample. © 2013 The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.
PubMed | Korea Promotion Institute for Traditional Medicine Industry
Type: Journal Article | Journal: The American journal of Chinese medicine | Year: 2016
Wound healing is a complex process orchestrated by the regeneration of the epithelium and the remodeling of the extracellular matrix through processes like collagen deposition. Galla Rhois has been widely used in traditional Korean medicine for its various pharmacological effects, including an anticoccidial effect, however, little is known about its healing activity. The purpose of this study was to determine the effects of Galla Rhois ethanol extract (GRE) on wound healing activities, including H2O2-induced oxidative stress, cell migration, and lactate dehydrogenase (LDH) release assays using human keratinocyte (HaCaT) and dermal fibroblasts (CCD-986SK). In addition, total soluble collagen deposition and collagen gene expression for TypeI and III collagen were evaluated in CCD-986SK. Total tannin and flavonoid contents for GRE were measured. GRE induced a significant increase in the number and migration of cells, along with a decrease in cell death and LDH release. In addition, it also induced the over-expression of collagen TypeI and III mRNA and caused increased synthesis of total soluble collagen. The contents of total tannin and flavonoid for GRE were 55.7% ([Formula: see text][Formula: see text]mg/g) and 62.9% ([Formula: see text][Formula: see text]mg/g), respectively. The results suggest that GRE can cause accelerated wound healing by increasing cell survival, proliferation, migration, and collagen synthesis along with a potential anti-oxidant property. This evidence provides novel insight into natural therapy for tissue injury.
PubMed | Jaseng Spine and Joint Research Institute and Korea Promotion Institute for Traditional Medicine Industry
Type: Journal Article | Journal: BMC complementary and alternative medicine | Year: 2016
Pharmacopuncture is a relatively new acupuncture therapy combining acupuncture with herbal medicine. While pharmacopuncture is applied extensively in Korean medicine treatment, there are no clinical reports regarding what types of pharmacopuncture are used for which diseases.Data was extracted retrospectively from the electronic medical records of all inpatients and outpatients at 12 Korean medicine hospitals and clinics during the period of December 17, 2010 to October 2, 2014. Treatment patterns for acupuncture, electroacupuncture and pharmacopuncture were analyzed. Principle diagnosis codes, frequency of treatment, pharmacopuncture type and costs were investigated to assess pharmacopuncture use in clinical settings.During the study period, a total 33,415 inpatients and 373,755 outpatients visited the study sites, and most were musculoskeletal. Among inpatients and outpatients, 98.6% and 77.6% received pharmacopuncture, respectively. Administration rate of pharmacopuncture for the 10 most frequent principle diagnosis codes was 97.2-99.3% in inpatients, and that for outpatients was 73.0-91.5%. The average number of pharmacopuncture sessions in pharmacopuncture recipients was 8.212.3 for outpatients and 25.818.7 for inpatients. The mean total cost for pharmacopuncture per patient was $556.24174.62 among inpatients, and $149.16243.85 among outpatients. Estimated average cost per pharmacopuncture session was $23-24 for inpatients, and $17-18 for outpatients. Shinbaro1, bee venom, Hwangryunhaedok, and Shinbaro2 were the most frequently used pharmacopuncture types.This is the first analysis of treatment patterns of pharmacopuncture in a large-scale Korean medicine hospital/clinic patient population. We verified patterns of pharmacopuncture use for musculoskeletal disease treatment in Korea, and use of pharmacopuncture varied depending on disease or symptom severity. These results are expected to contribute to future clinical study design and standardization of pharmacopuncture.