Kang H.-K.,Chonnam National University |
Ko E.-A.,Korea Kolmar Corporation |
Kim J.-H.,Chonnam National University |
Kim D.,Chonnam National University
Bioprocess and Biosystems Engineering | Year: 2013
The open reading frame of dsrE563, a dextransucrase gene obtained from a constitutive mutant (CB4-BF563) of Leuconostoc mesenteroides B-1299, consists of 8,511 bp encoding 2,836 amino acid residues. DsrE563 contains two catalytic domains (CD1 and CD2). Two truncated derivative mutants DsrE563ΔCD2Δ GBD (DsrE563-1) and DsrE563ΔCD2ΔVR (DsrE563-2) of DsrE563 were constructed and expressed using the pRSETC vector in Escherichia coli. The derivatives DsrE563-1 (deletion of 1,620 amino acids from the C-terminus) and DsrE563-2 (deletion of 1,258 amino acids from the C-terminus and 349 amino acids from the N-terminus) were expressed as active enzymes. Both enzymes synthesized less-soluble dextran, mainly containing α-1,6 glucosidic linkage. The synthesized less-soluble dextran also had a branched α-1,3 linkage. DsrE563-2 showed 4.5-fold higher dextransucrase activity than that of DsrE563-1 and showed higher acceptor reaction efficiency than that of dextransucrase from L. mesenteroides 512 FMCM when various mono or disaccharides were used as acceptors. Thus, the glucan-binding domain was important for both enzyme expression and dextransucrase activity. © 2013 Springer-Verlag Berlin Heidelberg.
Park J.-M.,GFC |
Han S.-K.,Korea Kolmar Corporation |
Lee D.-G.,GFC |
Kang H.,RandD center |
And 2 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014
A Gram-stain negative, non-flagellated, non-gliding and rod-shaped bacterium, strain JHH-2T, was isolated from seawater collected in Jeju Island, Korea. The novel isolate was found to grow at 25–30 °C, at pH 6.5–7.0 and in the presence of 1–2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JHH-2T is closely related to Siansivirga zeaxanthinifaciens JCM 17682T, with 16S rRNA gene sequence similarity of 96.6%. The predominant respiratory quinone was identified as menaquinone-6 and the major cellular fatty acids were detected as iso-C15:0, C16:0 and iso-C17:0 3-OH. Polar lipid profiling revealed the presence of phosphatidylethanolamine, an unidentified glycolipid, four unidentified aminolipids and two unidentified lipids. The DNA G+C content of the chromosomal DNA of the type strain is 35.6 mol%. On the basis of several distinct phenotypic characteristics and phylogenetic analysis, a new species of the genus Siansivirga, Siansivirga jejunensis JHH-2T sp. nov. is proposed. The type strain is JHH-2T (= KCCM 92030T = JCM 19228T). © 2014, Springer International Publishing Switzerland.
Kim J.-A.,Pukyong National University |
Ahn B.-N.,Pukyong National University |
Kong C.-S.,Silla University |
Park S.-H.,Korea Kolmar Corporation |
And 2 more authors.
Photodermatology Photoimmunology and Photomedicine | Year: 2012
Background/Purpose: In the present study, the effect of 3-5kDa chitooligosaccharide (COS) on homeostasis between the expression of collagen-degrading matrix metalloproteinases (MMPs) and collagen synthesis was investigated using ultraviolet (UV)-A irradiated dermal fibroblasts. Methods: UV protection imparted by 3-5kDa COS was measured by examining the UV absorption spectrum. Collagenase MMP secretion was examined using an enzyme-linked immunosorbent assay. The levels of collagenases and collagen synthetic markers were determined by employing the reverse transcriptase-polymerase chain reaction and Western blot analysis. Results: The 3-5kDa COS not only absorbed UV-A and UV-B light but also inhibited collagenase (MMP-1, MMP-8, and MMP-13) and gelatinase (MMP-2 and MMP-9) MMP expression. The suppression of MMP expression was found to be due to an increase in expression of the tissue inhibitors of MMP (TIMP)-1 and TIMP-2. Treatment with 3-5kDa COS enhanced collagen synthetic markers such as procollagen, type I, III, and IV collagens in UV-A-irradiated dermal fibroblasts. Furthermore, the effects of 3-5kDa COS on collagen degradation and collagen synthesis in UV-A irradiated dermal fibroblasts were regulated via the inhibition of activating protein-1 (AP-1) signaling. Conclusion: Our results suggest that 3-5kDa COS can be used to develop as topical applications for antiphotoaging cosmeceuticals as it enhances collagen synthesis. © 2012 John Wiley & Sons A/S.
Lee D.K.,Korea University |
Jeong K.Y.,Korea Kolmar Corporation
Bulletin of the Korean Chemical Society | Year: 2010
The lyotropic mesomorphism of lamellar liquid crystalline phase was examined by observing the swelling behavior of Distearoylphosphatidylcholine(DSPC) in glycerin and panthenol without water. The lyotropic mesomorphism was examined by using DSC, XRDs and Cryo-SEM. Increase of two polar solvents under non-hydrous condition showed distinctive differences in the lyotropic mesomorphism from forming different anisotropic structures with DSPC. Glycerin did not affect to the crystalline region of lamellar phase, whereas typical swelling mesomorphism was shown in the noncrystalline region. In contrast, panthenol showed some effect on the crystalline region, but common swelling mesomorphism was found in the non-crystalline region. In this case, the isopropyl and propyl groups in panthenol were the main factor to affect to the lipophilic domain in the crystalline region of lamellar phase. Also, it was found that the formation of well-arranged lamellar structure only by introducing glycerin and panthenol as a solvent without water, was possible. These results were confirmed by examination of the swelling mesomorphism of liquid crystal membrane triggered by introducing the two polar solvents.
Han S.K.,Korea Kolmar Corporation |
Kim Y.G.,GFC. Co. |
Kang H.C.,GFC. Co. |
Huh J.R.,GFC. Co. |
And 4 more authors.
Journal of the Korean Society for Applied Biological Chemistry | Year: 2014
Natural products with non-toxic and environmentally friendly properties are good sources for skin-whitening and brightening cosmetic agents. Strawberries (Fragaria ananassa), and their parts are used as cosmetic agents, because they contain high levels of bioactive substances. We isolated and identified compounds from F. ananassa calyx. Oleanolic acid has multiple biological activities, including anti-tumor, anti-angiogenic, antiinflammatory, anti-oxidant, and pro-apoptotic effects. However, no study has investigated the influence of oleanolic acid on melanin synthesis in B16-F10 melanoma cells. In the present study, we investigated the effect of oleanolic acid on melanin biosynthesis in B16-F10 melanoma cells stimulated with α-melanocyte stimulating hormone (α-MSH). Oleanolic acid-mediated melanogenesis inhibition was studied by measuring intracellular and secreted melanin levels and by using Western blot and semiquantitative reverse transcriptase-polymerase chain reaction analyses. Oleanolic acid suppressed melanin release and expression, resulting in a significant dose-dependent decrease in secreted and intracellular melanin levels and cellular tyrosinase activity. Furthermore, it inhibited the expression of melanogenesis-associated factors, including tyrosinase, tyrosinase-related proteins-1 and -2, and microphthalmia-associated transcription factor, in α-MSH-stimulated B16-F10 melanoma cells. The results of the present study can contribute to the development of cosmetic agents utilizing the skin whitening and brightening effect of oleanolic acid, which will likely have a wide range of applications in the cosmetic industry and/or clinical practice in the future. © 2014, The Korean Society for Applied Biological Chemistry.
Jeong K.-Y.,Korea Kolmar Corporation |
Lee D.-K.,Chungbuk National University
Applied Chemistry for Engineering | Year: 2010
This paper aims to investigate the lyotropic behaviors of DSPC and CER3 when they are swollen by GLY as a solvent. The analyses were carried out on DSC, XRDs, PM, and Cryo-SEM. CER3 which has its high crystallinity and structural similarity with DSPC was well arranged up to 7.0 wt% in comparison to 20 wt% DSPC without any separation, but it was separated from the liquid crystalline (LC) phase to form another crystalline phase with the expression of its characteristic peak in XRDs and eutectic thermal behavior in DSC. Introducing CER3, two types of patterns were shown in XRD spectra; one is SPP expressed in a normal LC and another is LPP expressed in human skin SC. Therefore, it was confirmed that the incorporation of CER3 makes LC structure more similar to human skin. In Cryo-SEM study, it was shown that CER3 makes LC structure thicker and denser.