Korea Institute for Animal Products Quality Evaluation

Sejong, South Korea

Korea Institute for Animal Products Quality Evaluation

Sejong, South Korea
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PubMed | National Institute of Animal Science, Konkuk University, Institute of Hanwoo, Experiment Research Institute and Korea Institute for Animal Products Quality Evaluation
Type: Journal Article | Journal: Reproduction in domestic animals = Zuchthygiene | Year: 2016

Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous-specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non-oestrous and controlled internal drug release (CIDR)-induced oestrous-stage mucus proteins were purified and subjected to surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI-TOF/TOF. Among differentially expressed proteins, lactoferrin (LF) and glutamate receptor-interacting protein 1 (GRIP1) showed a twofold increase during the CIDR-induced oestrous stage compared to the levels in non-oestrous stage in bovine cervical mucus. The RT-PCR, Western blotting and immunohistochemistry results showed that LF and GRIP1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine LF and GRIP1 exist during the oestrous stage, but not during the non-oestrous stage, suggesting that cervical mucus LF and GRIP1 are useful oestrous detection markers in cattle.


Lee S.-K.,Korea Institute for Animal Products Quality Evaluation | Lee S.-K.,Konkuk University | Chon J.-W.,Konkuk University | Song K.-Y.,Konkuk University | And 3 more authors.
Poultry Science | Year: 2013

To determine the prevalence of Salmonella serotype Enteritidis in eggs in South Korea, we conducted a microbiological survey of commercially available eggs produced in conventional or organic farms during the period from 2010 to 2012. The contents of 7,000 raw shell eggs (6,000 of conventional and 1,000 of organic origin) were examined to evaluate the extent and type of Salmonella Enteritidis contamination. A total of 26 salmonellae (7.4% of all pooled samples) were isolated from 350 homogenized pools, each containing the contents from 20 eggs. An unexpected and particularly surprising finding was that all the Salmonella isolates were serotyped as Salmonella Gallinarum. Salmonella Gallinarum was more common in eggs from organic farms: 10 of 50 egg pools (20.0%) from organic and 16 of 300 egg pools (5.3%) from conventional farms tested positive for Salmonella Gallinarum. However, organic and conventional isolates showed similar antimicrobial susceptibilities. All the isolates and a vaccine strain, SG 9R, which has been widely used in South Korea, were further characterized using the automated repetitive sequence-based PCR (rep-PCR) system, DiversiLab, to ascertain the molecular subtypes and to identify differences from the vaccine strain. The rep- PCR identified 2 distinct clusters among the 26 Salmonella Gallinarum isolates with a greater than 96% similarity index. These were clearly differentiated from the vaccine strain, SG 9R, with which there was a less than 86% similarity index. We found there was low genetic heterogeneity among isolates within each cluster and were able to distinguish wild type strains from the live vaccine strain (SG 9R) using the DiversiLab system. © 2013 Poultry Science Association Inc.


PubMed | Jeju National University, Changjobio Corporation, National Research Foundation NRF, Gyeongnam National University of Science and Technology and Korea Institute for Animal Products Quality Evaluation
Type: Journal Article | Journal: Biotechnology, biotechnological equipment | Year: 2015

Wood creosote, an herbal anti-diarrheal and a mixture of major volatile compounds, was tested for its non-toxicological effects, using a rat model, with the objective to use the creosote as an antibiotic substitute. A total of 30 Sprague-Dawley rats were studied to form five groups with 6 rats each. Korea beechwood creosote was supplemented into three test groups with 0.03g/kg, 0.07g/kg and 0.1g/kg body weight/day without antibiotic support, along with a positive control of Apramycin sulphate (at 0.5% of the daily feed) and a negative control. Korean beechwood creosote supplementation showed no negative effect on the body weight gain in comparison to the negative and the positive control groups and the feed conversion ratio was also comparable with that of the control groups. The clinical pathology parameters studied were also under the umbrella of normal range, including liver specific enzymes, blood glucose, total protein, blood urea nitrogen (BUN), which indicated no toxic effect of creosote at the given doses. The non-hepatotoxic effect was also confirmed using hepatic damage specific molecular markers like Tim-p1, Tim-p2 and Tgf-1. The results suggested that Korean beechwood may be used as antibiotic substitute in weanling pigs feed without any toxic effect on the body. Although the antimicrobial properties of creosote were not absolutely similar to those of apramycin sulphate, they were comparable.


Kim G.-D.,Jeju National University | Lee H.-S.,Korea Institute for Animal Products Quality Evaluation | Jung E.-Y.,Gyeongsang National University | Lim H.-J.,Gyeongsang National University | And 6 more authors.
Livestock Science | Year: 2013

The aim of this study is to assess the effect of CO2 gas stunning which has not been conducted until now in comparison with captive bolt stunning on the meat quality of cattle. A total of 40 steers were slaughtered following two stunning processes: CO2 gas stunning (n=20), exposure at 70% CO2 gas atmosphere for 140sec; and captive bolt stunning (CBS, n=20). The slaughtered steers were classified into Group A (620-710kg) and Group B (720-790kg) by their live weight. CO2 gas stunning decreases pH value (p<0.05) but increases lightness (p<0.001) and sarcomere length (p<0.001 and p<0.01 for Group A and Group B, respectively) in all live weight groups. Drip loss was increased with CO2 gas stunning in Group A (620-710kg of live weight) (p<0.05), whereas WBSF was decreased with CO2 gas stunning in Group B (720-790kg of live weight) (p<0.001). Therefore, CO2 gas stunning negatively affects muscle pH and water-holding capacity in steers but CO2 gas stunning can improve the tenderness and lightness compared with captive bolt stunning in cattle. © 2013 Elsevier B.V.


Bae Y.S.,Chungnam National University | Lee J.C.,Korea Institute for Animal Products Quality Evaluation | Jung S.,Chungnam National University | Kim H.-J.,Seoul National University | And 4 more authors.
Korean Journal for Food Science of Animal Resources | Year: 2014

This study was performed to evaluate the quality characteristics of three deboned categories of chicken thigh meat: one which was slaughtered and deboned in the same plant (fresh); one which was slaughtered, deboned, frozen, and thawed in the same plant (frozen-thawed); and the last which was slaughtered in a plant, deboned in a different plant, but then transferred to the original plant (fresh-outside). Surface color, drip loss, 2-thiobarbituric acid reactive substances (TBARS) value, sensory evaluation, and total aerobic bacterial counts of the chicken samples were determined. Moreover, the torrymeter was used to measure the differences in freshness of the chicken meat. The surface color and the TBARS values did not show significant differences among the three categories. However, the total aerobic bacterial counts of fresh-outside and frozenthawed chicken meat were significantly higher than the fresh chicken meat on the first storage day, and the drip loss of frozen-thawed chicken meat was significantly higher than the fresh-outside and fresh chicken meat. In addition, the sensory evaluation of frozen-thawed chicken meat was significantly lower than the fresh-outside and fresh chicken meat. Torrymeter values were higher in fresh chicken meat than fresh-outside and frozen-thawed chicken meat during the storage period. These results indicate that the quality of frozen-thawed chicken meat is comparatively lower than the fresh chicken meat, and the torrymeter values can accurately differentiate the fresh-outside and frozen-thawed chicken meat from the fresh ones.


Gil J.,Kangwon National University | Kim D.,Kangwon National University | Yoon S.-K.,Korea Institute for Animal Products Quality Evaluation | Ham J.-S.,South Korean National Institute of Animal Science | Jang A.,Kangwon National University
Korean Journal for Food Science of Animal Resources | Year: 2016

This study was conducted to evaluate the antioxidative and anti-inflammatory effects of boiled pork powder (BPP) and hot water extract powder (HWEP) from 4 cuts of meat from Landrace x Yorkshire x Duroc (LYD). The highest DPPH radical scavenging activities determined were from BPP of Boston butt (13.65 M TE) and HWEP of loin (19.40 M TE) and ham (21.45 M TE). The 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of BPP from shoulder ham (39.28 M TE) and ham (39.43 M TE) were higher than those of other meat cuts, while HWEP of ham exhibited the highest ABTS radical scavenging activity. A higher oxygen radical absorbance capacity was determined for BPP from ham (198.35 M TE) and in HWEP from loin (204.07 M TE), Boston butt (192.85 M TE), and ham (201.36 M TE). Carnosine content of BPP and HWEP from loin and were determined to be 106.68 and 117.77 mg/g on a dry basis, respectively. The anserine content of BPP (5.26 mg/g, dry basis) and HWEP (6.79 mg/g, dry basis) of shoulder ham exhibited the highest value as compared to the extracts from the other meat cuts. The viability of RAW 264.7 cells was increased with increasing HWEP from loin and ham treatment. In addition, the expression of IL-6 and TNF-α was significantly reduced by HWEP from loin and ham, in a dose dependent manner. These results suggested that boiled pork and hot water extract of pork have antioxidative and cytokine inhibitory effects. © 2016 Korean Society for Food Science of Animal Resources.


Choy Y.H.,South Korean National Institute of Animal Science | Park B.H.,South Korean National Institute of Animal Science | Choi T.J.,South Korean National Institute of Animal Science | Choi J.G.,South Korean National Institute of Animal Science | And 5 more authors.
Asian-Australasian Journal of Animal Sciences | Year: 2012

The objective of this study was to estimate economic weights of Hanwoo carcass traits that can be used to build economic selection indexes for selection of seedstocks. Data from carcass measures for determining beef yield and quality grades were collected and provided by the Korean Institute for Animal Products Quality Evaluation (KAPE). Out of 1,556,971 records, 476,430 records collected from 13 abattoirs from 2008 to 2010 after deletion of outlying observations were used to estimate relative economic weights of bid price per kg carcass weight on cold carcass weight (CW), eye muscle area (EMA), backfat thickness (BF) and marbling score (MS) and the phenotypic relationships among component traits. Price of carcass tended to increase linearly as yield grades or quality grades, in marginal or in combination, increased. Partial regression coefficients for MS, EMA, BF, and for CW in original scales were +948.5 won/score, +27.3 won/cm2, -95.2 won/mm and +7.3 won/kg when all three sex categories were taken into account. Among four grade determining traits, relative economic weight of MS was the greatest. Variations in partial regression coefficients by sex categories were great but the trends in relative weights for each carcass measures were similar. Relative economic weights of four traits in integer values when standardized measures were fit into covariance model were +4:+1:-1:+1 for MS:EMA:BF:CW. Further research is required to account for the cost of production per unit carcass weight or per unit production under different economic situations. Copyright © 2012 by Asian-Australasian Journal of Animal Sciences.


PubMed | Hanwoo Research Center, National Institute of Animal Science, Chonbuk National University and Korea Institute for Animal Products Quality Evaluation
Type: | Journal: Animal science journal = Nihon chikusan Gakkaiho | Year: 2016

To investigate the physico-chemical and sensory properties of striploin muscles, 90 Hanwoo carcasses (QG 1


Kim C.-H.,Korea Institute for Animal Products Quality Evaluation | Song J.-H.,Korea Institute for Animal Products Quality Evaluation | Lee J.-C.,Korea Institute for Animal Products Quality Evaluation | Lee K.-W.,Konkuk University
International Journal of Poultry Science | Year: 2014

The present study was conducted to monitor age-related changes in egg quality during the entire production cycle of laying hens. Thirty eggs were sampled from the commercial farm on a weekly basis for egg analysis. In order to increase in confidence in sampling, farm personnel were educated to collect the eggs from the pre-determined cages during the whole period and all layers were subjected to an identical farm management and fed a commercial layer diet. In total, 1,470 eggs were sampled and transported to the laboratory of Korea Institute for Animal Products Quality Evaluation. The parameters for egg quality included egg weight, eggshell color, eggshell breaking strength, eggshell thickness, Haugh unit and yolk color. It was shown that egg weight increased as the hen aged. Eggshell breaking strength gradually decreased, but eggshell thickness was randomly scattered without showing any age-related trends during the laying period. The intensity of eggshell color kept relatively constant while yolk color increased with age. Finally, Haugh unit as an indicator of freshness of eggs was consistently decreased as the hens aged. In this study, we noticed that eggshell breaking strength and egg weight were negatively correlated (r= - 0.500, p<0.001). Eggshell color did not significantly correlate with eggshell thickness (r = -0.074, p>0.50), but marginally and positively correlated with egg weight (r = 0.248, p = 0.082). It is concluded that among the various egg quality parameters analyzed, egg weight, eggshell breaking strength or Haugh unit reduced as the hens aged. Further study is needed to include various parameters of egg quality such as chemical or nutritional composition which will provide more insight into the age-mediated changes in egg quality. © Asian Network for Scientific Information, 2014.


PubMed | Korea Institute for Animal Products Quality Evaluation
Type: Journal Article | Journal: Poultry science | Year: 2013

To determine the prevalence of Salmonella serotype Enteritidis in eggs in South Korea, we conducted a microbiological survey of commercially available eggs produced in conventional or organic farms during the period from 2010 to 2012. The contents of 7,000 raw shell eggs (6,000 of conventional and 1,000 of organic origin) were examined to evaluate the extent and type of Salmonella Enteritidis contamination. A total of 26 salmonellae (7.4% of all pooled samples) were isolated from 350 homogenized pools, each containing the contents from 20 eggs. An unexpected and particularly surprising finding was that all the Salmonella isolates were serotyped as Salmonella Gallinarum. Salmonella Gallinarum was more common in eggs from organic farms: 10 of 50 egg pools (20.0%) from organic and 16 of 300 egg pools (5.3%) from conventional farms tested positive for Salmonella Gallinarum. However, organic and conventional isolates showed similar antimicrobial susceptibilities. All the isolates and a vaccine strain, SG 9R, which has been widely used in South Korea, were further characterized using the automated repetitive sequence-based PCR (rep-PCR) system, DiversiLab, to ascertain the molecular subtypes and to identify differences from the vaccine strain. The rep-PCR identified 2 distinct clusters among the 26 Salmonella Gallinarum isolates with a greater than 96% similarity index. These were clearly differentiated from the vaccine strain, SG 9R, with which there was a less than 86% similarity index. We found there was low genetic heterogeneity among isolates within each cluster and were able to distinguish wild type strains from the live vaccine strain (SG 9R) using the DiversiLab system.

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