Korea INSpharm Research Institute

Hwasun, South Korea

Korea INSpharm Research Institute

Hwasun, South Korea

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Ha Y.,Kyung Hee University | Kim O.-K.,Kyung Hee University | Nam D.-E.,Kyung Hee University | Kim Y.,Korea INSPharm Research Institute | And 3 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2015

The present study investigated the immunomodulatory effects of Curcuma longa L. ethanol extracts on natural killer (NK) cells and T cells. We treated Curcuma longa L. ethanol extracts at concentrations of 20, 50, 100, and 150 μg/mL to murine NK cells co-incubated with YAC-1 cells. Curcuma longa L. ethanol extracts resulted in increased NK cell activity compared to the control group at all concentrations. In the groups treated with Curcuma longa L. ethanol extracts, CD69 and IFN-γ expression levels significantly increased compared to the control group at 100 and 150 μg/mL. In addition, Curcuma longa L. ethanol extracts induced significant elevation of CD8+ T cell numbers in a dose-dependent manner. However, Curcuma longa L. ethanol extracts also led to reduction of CD4+ T cell and MHCⅡ numbers. The findings of this study suggest that Curcuma longa L. ethanol extracts could enhance the immune response through activation of NK and cytotoxic T cells due to a proliferative shift of antigen presentation from MHCⅡ to MHCⅠ, presumably. © 2015, Korean Society of Food Science and Nutrition. All rights reserved.


You M.-K.,Mokpo National University | Kim M.-S.,Hisol | Jeong K.-S.,Kyungpook National University | Kim E.,Korea INSpharm Research institute | And 2 more authors.
Nutrition Research and Practice | Year: 2016

BACKGROUND/OBJECFTIVES: The present study was conducted to examine the inhibitory effect of loquat leaves on MDA-MB-231 cell proliferation and invasion. MATERIALS/METHODS: Female athymic nude mice were given a subcutaneous (s.c.) inoculation of MDA-MB-231 cells and randomly grouped to receive a s.c. injection of either 500 mg/kg ethanol, water extract or vehicle five times a week. Tumor growth, mitotic rate and necrosis were examined. MDA-MB-231 cells were cultured with DMSO or with various concentrations of loquat water or ethanol extract. Proliferation, adhesion, migration, invasion and matrix metalloproteinase (MMP) activity were examined. RESULTS: Tumor growth of xenograft nude mouse was significantly reduced by loquat extracts. The results of mitotic examination revealed that loquat extracts reduced tumor cell division. Both ethanol and water extracts significantly inhibited MDA-MB-231 cell proliferation. The protein expression of ErbB3 was significantly down-regulated by loquat leaf extracts. Loquat leaf extracts increased apoptosis of MDA-MB-231 cells following 24 hour incubation and the ethanol extract was more potent in inducing apoptosis than the water extract. Furthermore, loquat extracts inhibited adhesion, migration and invasion of MDA-MB-231 cells. MMP activity was significantly inhibited by loquat extracts. CONCLUSION: Our results show that extracts of loquat inhibit the growth of tumor in MDA-MB-231 xenograft nude mice and the invasion of human breast cancer cells, indicating the inhibition of tumor cell proliferation and invasion. © 2016 The Korean Nutrition Society and the Korean Society of Community Nutrition.


Kim M.-J.,Yonsei University | Lee J.,Kyung Hee University | Seong A.-R.,Yonsei University | Lee Y.-H.,University of Suwon | And 5 more authors.
Food and Chemical Toxicology | Year: 2011

The generation of oxygen free radicals and oxidative damage is believed to be involved in the pathogenesis of neurodegenerative disorders. Eriobotrya japonica has been used to treat several diseases in East Asia. In this study, we investigated the protective effect of an E. japonica extract against Aβ peptide-induced oxidative stress. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay demonstrated that the E. japonica extract scavenged approximately 40% of DPPH radicals. Also, treatment of the E. japonica extract inhibited Aβ1-42-mediated neuronal cell death. Furthermore, treatment of E. japonica extract efficiently suppressed the increase in intracellular ROS triggered by the Aβ1-42 peptide. Importantly, mice pre-treated with the E. japonica extract showed restoration of alternation behavior and reversal of Aβ1-42-induced memory impairment.Consequently, the E. japonica extract substantially inhibited the increase in lipid peroxidation and restored superoxide dismutase activity. These results suggest that E. japonica protects from oxidative stress and cognitive deficits induced by the Aβ peptide. © 2010 Elsevier Ltd.


Kim M.S.,Mokpo National University | You M.K.,Mokpo National University | Rhyu D.Y.,Mokpo National University | Jeong K.S.,Kyungpook National University | And 3 more authors.
Food Science and Biotechnology | Year: 2011

This study was to demonstrate whether loquat leaves protect against 7,12-dimethylbenz[α]anthracene (DMBA)-induced breast cancer in female rats. Rats were fed control diet (NC, DC), ethanol (DE), or water extract of loquat leaves (DW) until sacrifice. At 50 days of age, animals in the DC, DE, and DW groups were given a single intra-gastric dose of DMBA (50 mg/kg) dissolved in corn oil. Both the water and ethanol extracts of loquat leaves decreased the incidence of tumors, with water extract having the higher inhibitory effect. Ethanol extract significantly decreased the multiplicity of tumors. Loquat extracts significantly decreased tumor cell proliferation and the tumor grade based on the degree of tubule formation. These results suggest that both water and ethanol extracts of loquat leaves inhibit the development of breast cancer. Such inhibition may be based on the suppression of initiation and tumor cell proliferation. © KoSFoST and Springer 2011.


Yoo S.A.,Kyung Hee University | Kim O.K.,Kyung Hee University | Nam D.-E.,Kyung Hee University | Kim Y.,Korea INSPharm Research Institute | And 3 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2014

Curcuma longa L. (CL) is a well known traditional medicinal plant that is also used in curries and mustards as a coloring and flavoring agent. However, CL is not usually used as a food source due to its bitter taste. We investigated the immunomodulatory effect of CL fermented by Aspergillus oryzae (FCL) on RAW 264.7 cells. FCL was extracted with cold water (CW), hot water (HW), 20% ethanol (20% EtOH) and 80% ethanol (80% EtOH), after which its effects on phagocytic activity, tumor necrosis factor-alpha (TNF-α), nitric oxide (NO) production, natural killer (NK) cell activity and mRNA expression of LP-BM5 eco were investigated. Phagocytic activity was increased in HW and 20% EtOH when compared to the control. The secretion of nitric oxide (NO) from RAW 264.7 cells did not change significantly relative to the control. However, TNF-α was significantly increased by the addition of FCL extracts. Moreover, FCL 20% ethanol extract showed a four fold increase in NK cell cytotoxity relative to the control group. Finally, we observed suppressed mRNA expression of LP-BM5 eco in FCL extracts, especially in the 20% ethanol extracts group. These results indicate that the FCL extracts can be used as a functional material due to their effective immunomodulating activities.


Kim O.K.,Kyung Hee University | Yoo S.A.,Kyung Hee University | Nam D.-E.,Kyung Hee University | Kim Y.,Korea INSPharm Research Institute | And 4 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2014

The immune system protects the body against harmful substances and infectious agents. Normally, the body can maintain a state of immune homeostasis. However, failure of immune homeostasis results in severe diseases when the immune system is defective. We investigated the immunomodulatory effect of Curcuma longa L. extract in LP-BM5 MuLV (murine leukemia viruses)-induced murine AIDS (acquired immune deficiency syndrome). Mice were divided into six groups: normal control, infected control (LP-BM5 MuLV infection), positive control (LP-BM5 MuLV infection+dietary supplement of red ginseng 200 mg/kg), CL50 (LP-BM5 MuLV infection+dietary supplement of Curcuma longa L. 20% alcohol extract 50 mg/kg), CL200 (LP-BM5 MuLV infection+dietary supplement of Curcuma longa L. 20% alcohol extract 200 mg/kg), and CL500 (LP-BM5 MuLV infection+dietary supplement of Curcuma longa L. 20% alcohol extract 500 mg/kg). We found that dietary supplementation with Curcuma longa L. 20% alcohol extract inhibited elevation of spleen, lymph node, and liver weights as well as reduction of T- and B-cell proliferation and natural killer cell activity induced by LP-BM5 MuLV infection. Moreover, Curcuma longa L. 20% alcohol extract inhibited Th1 (IL-2, IFN-γ)/Th2 (IL-4, IL-10) cytokine imbalance and pro-inflammatory cytokine production. In conclusion, these data suggest that Curcuma longa L. has immunomodulatory effects in LP-BM5 MuLV-induced murine AIDS. © 2014, Korean Society of Food Science and Nutrition. All rights reserved.


PubMed | Korea INSpharm Research institute, Kyungpook National University, Mokpo National University and Hisol
Type: Journal Article | Journal: Nutrition research and practice | Year: 2016

The present study was conducted to examine the inhibitory effect of loquat leaves on MDA-MB-231 cell proliferation and invasion.Female athymic nude mice were given a subcutaneous (s.c.) inoculation of MDA-MB-231 cells and randomly grouped to receive a s.c. injection of either 500 mg/kg ethanol, water extract or vehicle five times a week. Tumor growth, mitotic rate and necrosis were examined. MDA-MB-231 cells were cultured with DMSO or with various concentrations of loquat water or ethanol extract. Proliferation, adhesion, migration, invasion and matrix metalloproteinase (MMP) activity were examined.Tumor growth of xenograft nude mouse was significantly reduced by loquat extracts. The results of mitotic examination revealed that loquat extracts reduced tumor cell division. Both ethanol and water extracts significantly inhibited MDA-MB-231 cell proliferation. The protein expression of ErbB3 was significantly down-regulated by loquat leaf extracts. Loquat leaf extracts increased apoptosis of MDA-MB-231 cells following 24 hour incubation and the ethanol extract was more potent in inducing apoptosis than the water extract. Furthermore, loquat extracts inhibited adhesion, migration and invasion of MDA-MB-231 cells. MMP activity was significantly inhibited by loquat extracts.Our results show that extracts of loquat inhibit the growth of tumor in MDA-MB-231 xenograft nude mice and the invasion of human breast cancer cells, indicating the inhibition of tumor cell proliferation and invasion.


Oh J.,Mokpo National University | Min O.-J.,Mokpo National University | Kim H.,Mokpo National University | Kim Y.J.,Korea INSpharm Research Institute | And 2 more authors.
Journal of Applied Biological Chemistry | Year: 2011

Anti-obesity effects of Eriobotrya japonica leaf extracts were evaluated in 3T3-L1 adipocytes and ob/ob mice. Extracts of E. japonica were prepared with various solvents such as 80% methanol (MeOH), 50% ethanol (EtOH), and water using fresh or processed leaves. 80% MeOH, 50% EtOH, and processed water extracts of E. japonica leaves effectively inhibited lipid accumulation and expression of adipogenic transcription factors, peroxisome proliferator-activated receptor (PPAR)γ and CCAAP/enhancer-binding protein (C/EBP)α in 3T3-L1 adipocytes. Oral treatment with 50% EtOH extract of E. japonica at 200 or 400 mg/kg body weight for 12 weeks significantly reduced the body weight and plasma triglyceride level compared to those of control group, ob/ob mice. Food efficiency ratio (FER) was significantly suppressed after treatment with E. japonica processed water extract in ob/ob mice. The plasma protein level of adiponentin was highly increased by feeding of 400 mg/kg body weight of 50% EtOH extract of E. japonica to ob/ob mice. Thus, these results suggest that the 50% EtOH extract of E. japonica has an anti-obesity effect through regulation of adipogenic transcription factors, and plasma lipid and adiponectin levels in 3T3-L1 adipocytes and ob/ob mice.

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