News Article | April 25, 2017
Membrane proteins make up approximately a quarter of all gene products and are the targets of over 50% of all modern pharmaceutical drugs. The inner mitochondrial membrane (IMM) proteome plays a central role in maintaining mitochondrial physiology and cellular metabolism. Despite their importance, there has been no method to reveal the topology of mitochondrial membrane proteins in live cells, until now. A recent study, affiliated with UNIST has developed a new technique to understand the correct architectures of IMM proteins, using special chemical tools. By solving the most difficult stage of new drug development, their work will help speed the development of new therapeutics and cures. This research has been led by the team of Professor Hyun-Woo Rhee of Chemistry at UNIST in collaboration with Professor Jong-Seo Kim at Center from the Center for RNA Research, within the Institute for Basic Science (IBS) at Seoul National University and Professor Jeong-Kon Seo of UNIST Central Research Facilities (UCRF). The results of the study have been appeared in the March 15th edition of the Journal of the American Chemical Society (JACS). IMM is one of the most active sites for cellular metabolism and it is deeply related for various human metabolic diseases including cancer and neurodegenerative diseases. Therefore, it is crucial to understand the correct architecture of the IMM proteome in live cells for successful and efficient development of mitochondria-targeted therapeutics. In the study, Professor Lee and his research team revealed the in vivo topological direction of 135 IMM proteins, using an in situ-generated desthiobiotin-phenoxyl radical probe with genetically targeted peroxidase (APEX). "The determination of membrane protein structure is one of the most challenging tasks in protein structure analysis," says Professor Lee. "Our identification of structural information on the mitochondrial inner-membrane proteome can provide valuable insights for the architecture and connectome of the IMM proteome in live cells." The research team designed a new chemical probe, desthiobiotin-phenol and applied it to the IMM proteins in live cells. Then, they identified the structure of membrane proteins via mass spectrometry (MS). Peroxidase can make phenoxyl radical when react with hydrogen peroxide. Then, phenoxyl radical can react with tyrosine residue on the proximal protein forming covalent bond. In the study, the research team obtained the topology information by analyzing labeled tyrosine site of membrane protein. The majority of protein sequence analysis today uses mass spectrometry (MS), which digests the protein sample into peptides using an appropriate enzyme. Previous analyses, which used genetically-targeted ascorbate peroxidase (APEX) could not resolve structural identification because these analyses performed based on the unlabeled peptide detection. However, only labeled peptide can provide useful structural information, according to the research team. Unlike biomolecules that are labeled with biotin-phenol, proteins and other targets that are labeled with desthiobiotin-phenol can be eluted without harsh, denaturing conditions. Moreover, as the number of available membrane protein structure samples, obtained via MS increases, the efficiency of structural identification of membrane proteins also increases. Owing to the short lifetime of phenoxyl radicals generated in situ by submitochondrial targeted APEX and the impermeability of the IMM to small molecules, the solvent-exposed tyrosine residues of both the matrix and intermembrane space (IMS) sides of IMM proteins were exclusively labeled with the radical probe in live cells by Matrix-APEX and IMS-APEX, respectively and identified by mass spectrometry. Through this analysis, the research team confirmed 58 IMM protein topologies and determine the topological direction of 77 IMM proteins whose topology at the IMM has not been fully characterized. This work has been supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare of Korea. And also supported by the Institute for Basic Science from the Ministry of Science, ICT, and Future Planning of Korea. Song-Yi Lee, et al., "Architecture Mapping of the Inner Mitochondrial Membrane Proteome by Chemical Tools in Live Cells", JACS, (2017).
News Article | December 1, 2016
TORONTO, ON--(Marketwired - December 01, 2016) - Today MaRS Innovation (MI) and the Korea Health Industry Development Institute (KHIDI) signed a five year global partnership agreement to commercialize the latest advances in biomedical and healthcare innovations which is expected to generate new startups worth over $100M. This agreement will promote the co-development and commercialization of medical technologies and therapeutics between Ontario and South Korea in concert with industry and government. It will provide the resources to develop and implement novel technological solutions for today's most urgent healthcare problems and create economic benefits for both jurisdictions. Under the agreement, a joint commercialization platform with an infrastructure of collaboration will help Ontario and South Korean biomedical companies and health institutions commercialize and launch their products in each other's markets. KHIDI and the Top 10 South Korean research-driven hospitals have already expressed interest to collaborate with MaRS Innovation's 15 institutions including the world renowned University of Toronto and its nine affiliated teaching hospitals. By securing global-level research capabilities and creating positive outcomes through commercialization initiatives, MaRS Innovation anticipates that this partnership will create 10 startup companies and over 100 highly skilled jobs in both countries. "KHIDI has a proven track record of identifying promising early stage biomedical and healthcare projects and successfully developing them through clinical stages," stated Dr. Raphael Hofstein, President and CEO, MaRS Innovation. "We believe that our joint pools of novel technologies and projects together with KHIDI's medical development expertise and strategic collaborations with global players is a perfect match." The highlight of the agreement centers on a new global high-tech KHIDI Biomedical Business Hub in Seoul which MaRS Innovation will help to transform into a growth engine for South Korea's economy. Similar to Toronto's world class biomedical and healthcare cluster, the KHIDI Biomedical Business Hub will become one of a handful of world leading biomedical and healthcare clusters. KHIDI President Young Chan Lee said, "We are pleased to collaborate with MaRS Innovation, one of the world's leading commercialization centres, to transform South Korea's biomedical and healthcare research strengths into commercial opportunities through the newly created KHIDI Biomedical Business Hub and other initiatives. We view them as a valuable source of potential innovative technologies and look forward to working together towards the development of future therapies." This agreement represents a new era of collaboration to expand the knowledge-based economies of South Korea and Ontario, Canada. Dr. Lincoln Kim, CEO of ICE Ventures said, "We are pleased to have arranged this global partnership with the support of the Ontario, Canadian, and South Korean governments and are excited to work with MaRS Innovation and KHIDI to implement this innovative collaboration and ensure its long term success." MaRS Innovation is the commercialization agent for 15 of Ontario's leading academic institutions including the University of Toronto and its affiliated research hospitals, York University, and Ryerson University. Supported by the Government of Canada through the Networks of Centres of Excellence, by the Government of Ontario through the Ontario Centres of Excellence, and by its members, MaRS Innovation is a transformational partnership that turns research strengths into commercial opportunities through industry partnerships, licensing and company creation. MaRS Innovation represents over $1.47 billion of R&D funding annually, a portfolio of 60 companies, $160M in outside risk capital, and is credited with creating over 400 jobs. The Korea Health Industry Development Institute (KHIDI) is Korea's only public organization set up in 1999 with sights on promoting and developing the health industry in South Korea, while promoting national healthcare services. Since its establishment, KHIDI has played a major role in the development of the nation's health industry. Apart from its primary function in Health Technology R&D, KHIDI has also taken the initiative in increasing competitiveness in different areas of the health industry, including pharmaceuticals, medical devices, food and beauty & cosmetics. ICE Ventures is a global commercialization company specializing in bringing next generation technologies to market by leveraging its networks in North America, Asia and Europe. It provides integrated solutions from deal brokering to business development and is recognized for creating global-scale public-private partnerships at the highest levels of government and industry. ICE Ventures currently manages a diversified commercial portfolio of biomedical/healthcare, industrial, and biometric technologies.
Song H.J.,Hallym University |
Song H.J.,Harvard University |
Cho Y.G.,Inje University |
Lee H.-J.,Korea Health Industry Development Institute
Metabolism: Clinical and Experimental | Year: 2013
Objective There has been no convincing evidence for a direct relation between sodium intake and being overweight. Therefore, we investigated the independent relationship between overweight and sodium intake in human subjects. Methods Of those aged 19 to 64 years who participated in the 4th Korea National Health and Nutrition Examination Survey, a total of 5955 participants (54% female) were included. Subjects were excluded if they reported unrealistic daily total energy intakes or intentional dietary changes, were pregnant, or were diagnosed with hypertension, diabetes, renal failure, liver cirrhosis, or thyroid disease. Overweight was defined as having a body mass index of 25 kg/m2 or higher. Sodium intake was calculated from 24-h recall and categorized into quintiles. Multivariate logistic regression was used to test the relationship between sodium intake and being overweight. Results Compared to men in the lowest quintile, men in the 4th and 5th quintiles had an increased risk of being overweight with odds ratios (ORs) of 1.37 (95% confidence interval [CI], 1.02-1.82) and 1.67 (95% CI, 1.23-2.27) respectively, after adjusting for confounding factors including soft drink and energy intake. The P value for trend of ORs in each quintile for men was 0.0033. In women, compared to the lowest quintile, the highest quintile had an OR of 1.31(95% CI, 0.96-1.79) and showed a marginally significant trend towards increasing risk of being overweight (P value =.058). Conclusions Our findings suggest an independent relationship between high sodium intake and an increased risk of being overweight in adults. © 2013 Elsevier Inc.
Chin Y.R.,Korea Health Industry Development Institute |
Lee H.Y.,Dongseo University |
So E.S.,Seoul National University
International Journal of Public Health | Year: 2011
Objective: This study investigates suicidal ideation and its associated factors by sex in Korean adults, focusing on health behaviors and health status. Methods: Cross-sectional data of 6,969 adults (25-64 years) who completed the Fourth Korean National Health and Nutrition Examination Survey were analyzed. Results: In both sexes, those who were married, had higher educational attainment or were non-smokers experienced low stress and had good self-rated health showed lower suicidal ideation. Significant factors were age for men and subjective body image and sleep time for women. In men, those aged 45-54 years showed the highest suicidal ideation rate. The effects of stress and depression on suicidal ideation were higher in men than in women. Conclusions: The development of a suicide prevention program for Korean adults requires different approaches for each sex. For working men aged 45-54 years, it should focus on the management of work-related stress and depression. For women, it should be a community support program for those who are less educated, have no job or experience a great deal of stress and depression. © Swiss School of Public Health 2011.
Suh H.-J.,Sun Moon University |
Choi S.,Korea Health Industry Development Institute
Journal of Food and Nutrition Research | Year: 2012
The aim of this study was to determine the artificial colours in foods and evaluate the dietary intake of artificial colour additives in foods commonly consumed by different age groups in Korea. The content level of artificial food colour was experimentally determined by high performance liquid chromatography with a photodiode array detector. Of the 643 food items analysed, 503 (approximately 78%) contained artificial colorants. Consumers aged 13-19 years displayed the greatest consumption of colour additive-containing foods, but the amount was much lower than the acceptable daily intakes (ADI) established by FAO/WHO Joint Expert Committee on Food Additives (JEFCA). Both mean and high (95th percentile) intakes of permitted artificial colour additives for all population groups were markedly lower than the ADI, because the Korean food industry has widely substituted natural colour additives for artificial colorants. However, the issue of consumption of colour additives by targeted groups such as children is of concern, so further studies to provide data on dietary intake of artificial colour additives on such vulnerable groups are needed. © 2012 VúP Food Research Institute.
Lee H.-S.,Korea Health Industry Development Institute |
Duffey K.J.,University of North Carolina at Chapel Hill |
Popkin B.M.,University of North Carolina at Chapel Hill
Journal of Human Hypertension | Year: 2013
We examined major trends and patterns regarding sodium and potassium intake and the ratio of sodium and potassium in the diets of South Koreans. We used data from 24-h dietary recall data from 10 267, 8819 and 9264 subjects ages ≥2 years in the 1998, 2005 and 2009 Korean National Health and Nutrition Examination Surveys, respectively. Mean sodium intake did not change significantly between 1998 and 2009 (4.6 vs 4.7 g per day), while potassium intake increased significantly (2.6 vs 2.9 g per day (P<0.001)). The major dietary sodium sources were kimchi, salt, soy sauce and soybean paste, and most potassium came from unprocessed foods (white rice, vegetables, kimchi and fruits). About 50% of the participants consumed ≥4 g of sodium per capita per day. The proportion of respondents consuming four to six grams of potassium per capita per day increased from 10.3% in 1998 to 14.3% in 2009 (P<0.001), and the sodium-potassium ratio decreased from 1.88 to 1.71 (P<0.001). One major implication is that efforts to reduce sodium in processed foods will be ineffective and future efforts must focus on both education to reduce use of sodium in food preparation and sodium replacement in salt, possibly with potassium. © 2013 Macmillan Publishers Limited All rights reserved.
Lee H.-S.,Korea Health Industry Development Institute |
Duffey K.J.,University of North Carolina at Chapel Hill |
Popkin B.M.,University of North Carolina at Chapel Hill
Asia Pacific Journal of Clinical Nutrition | Year: 2012
Korea has undergone a major opening of its food markets and economy in the past decade. Little is understood about the impact of these shifts on the diet of Koreans. This analysis studies the shifts in consumption of foods between 1998 and 2009 to provide a thorough understanding of the transition and insights into directions in the next decades in Korea. Data are from the Korea National Health and Nutrition Examination Survey (KNHANES). The sample used was a nationally representative sample of individuals age ≥2 in 1998 and 2009 (n=10,267 and 9,264, respectively). The data was corrected for seasonality, and the original raw food data was regrouped into 53 food groups. SAS was used to adjust for design effects and weight. Despite a decade of efforts to increase whole grains intake, and fruit and vegetable intake, the mean intake of whole grains increased only a small amount (16 kcal/person/day); however, the proportion consuming any whole grains doubled from 24% to 46.3%. Rice declined significantly, and several important less healthful food trends emerged: total alcohol intake increased from 39 kcal/person/day to 82 kcal/person/day. Also, energy from sugar-sweetened beverages increased among teens and energy from tea and coffee increased among adults. Remarkably, compared to other Asian countries and a general worldwide trend, vegetable intake remained relative high in South Korea during this last decade while fat energy increased modestly from relative low levels. Dynamic causes of these trends and the government's response are discussed.
Kim H.,Korea Health Industry Development Institute
Journal of the Korean Medical Association | Year: 2014
Health Technology Research and Development (R&D) national grant system has been progressed by the unique governance of each countries. From this reason, it has historical context and path-dependency. South Korea, also have started their investment from Bio Technology Master Plan of Ministry of Science and Technology (1994) and Korea Health Technology R&D Project of Ministry of Health and Welfare (1995), currently investing around 1.1 trillion won (in the year 2012) into the Ministry of Education, Science and Technology (now, Ministry of Science, ICT and Future Planning), Ministry of Health and Welfare and Ministry of Knowledge Economy (now, Ministry of Trade, Industry and Energy). This paper focuses on the Health Technology R&D national grant system in the aspect of governance, mid-long term strategic plan, main program, planning-evaluation-management system. And last but not least, implications. First, there would be important to build the effective governance, performance based policy and administration mechanisms of national grant system for the Health Technology R&D. Second, main objective of Health Technology R&D, which is improving the quality of life for the public, could not meet their target practically. Third, R&D investment has been enlarged from treatment based to prevention and care focused and from technology-centered into demand-centered, which based on the unmet health needs. Last, most of Health Technology R&D project could not consider sustainable health system, which would be the most important value in healthcare.
Lim D.,Korea Health Industry Development Institute |
Ha M.,Health Management Technology |
Ha M.,Dankook University |
Song I.,Korea Health Industry Development Institute
Journal of Korean Medical Science | Year: 2014
This study aimed to analyze trends in the 10 leading causes of death in Korea from 1983 to 2012. Death rates were derived from the Korean Statistics Information Service database and age-adjusted to the 2010 population. Joinpoint regression analysis was used to identify the points when statistically significant changes occurred in the trends. Between 1983 and 2012, the age-standardized death rate (ASR) from all causes decreased by 61.6% for men and 51.2% for women. ASRs from malignant neoplasms, diabetes mellitus, and transport accidents increased initially before decreasing. ASRs from hypertensive diseases, heart diseases, cerebrovascular diseases and diseases of the liver showed favorable trends (ASR % change: -94.4%, -53.8%, -76.0%, and -78.9% for men, and -77.1%, -36.5%, -67.8%, and -79.9% for women, respectively). ASRs from pneumonia decreased until the mid- 1990s and thereafter increased. ASRs from intentional self-harm increased persistently since around 1990 (ASR % change: 122.0% for men and 217.4% for women). In conclusion, death rates from all causes in Korea decreased significantly in the last three decades except in the late 1990s. Despite the great strides made in the overall mortality, temporal trends varied widely by cause. Mortality trends for malignant neoplasms, diabetes mellitus, pneumonia and intentional self-harm were unfavorable. © 2014 The Korean Academy of Medical Sciences.
Lee Y.,Korea Health Industry Development Institute |
Chang H.,Kyung Hee University
Healthcare Informatics Research | Year: 2012
Objectives: Korea has one of the most advanced information technology (IT) infrastructures in the world, and the application of IT in health systems is rapidly progressing from computerization to information systems, ubiquitous systems, and smart systems. This study aims to analyze Korean environments in regards to the development of their u-Health industry and propose directions for u-Healthcare services based on this analysis. Methods: This paper reviews the background, progress history, and current status of u-Health in Korea, and suggests strategies for the u-Health industry based on an analysis of its barriers and obstacles. Results: When u-Health was introduced to Koreans, their policies and approaches focused mainly on environmental factors, yet these efforts have not progressed further to impact the u-Healthcare service industry itself. To develop the u-Healthcare industry, four points need to be considered: the development and support of the practical service model, institutional support, support of core technology and industry, and the institutionalization of health management service. Conclusions: Korea is at a strategic point to start building u-Healthcare service delivery models. u-Healthcare is a healthcare service that provides added value through u-Health environments. By identifying critical success factors in u-Healthcare, we can strengthen the u-Health industry and implement policies to coordinate our efforts in the process of value chains to which we belong. © 2012 The Korean Society of Medical Informatics.