Korea Ginseng Corporation Central Research Institute

Daejeon, South Korea

Korea Ginseng Corporation Central Research Institute

Daejeon, South Korea
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Jung Y.H.,Kyungpook National University | Park K.Y.,Kyungpook National University | Jeon J.H.,Kyungpook National University | Kwak Y.-S.,Korea Ginseng Corporation Central Research Institute | And 4 more authors.
Journal of Ginseng Research | Year: 2011

Red ginseng saponin fraction-A (RGSF-A) contains a high percentage of panaxadiol saponins that were isolated from Korean red ginseng by ultrafltration. The aim of this study was to elucidate the effects of RGSF-A on the porcine distal left anterior descending (LAD) coronary artery. The relaxant responses to RGSF-A were examined during contractions induced by 100 nM U46619 (9,11-dideoxy-9a,11a-methanoepoxy-prostaglandin F2a), a stable analogue of thromboxane A2. RGSF-A dose-dependently induced biphasic (fast- and slow-) relaxation in the distal LAD coronary artery in the presence of an intact endothelium. The fast-relaxation was quickly achieved in a minute, and then the slow-relaxation was slowly developed and sustained for more than thirty minutes after the administration of RGSF-A. The slow-relaxation had a tendency to be bigger than the fast-relaxation. Fast relaxation induced by RGSF-A was almost blocked by N ω-Nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase synthase inhibitor and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a guanylate cyclase inhibitor. However slow relaxation induced by RGSF-A was only partially inhibited by L-NAME and ODQ. In the endothelium-removed ring, RGSF-A evoked only slow-relaxation to a certain extent. These data suggest that RGSF-A induced both endothelium dependent fast- and slow-relaxation and endothelium independent slow-relaxation in the porcine distal LAD coronary artery. The endothelium dependent fast-relaxation is mediated by the nitric oxide (NO)-cGMP pathway, and the endothelium dependent slow-relaxation is at least partially mediated by the NO-cGMP pathway. However, the endothelium-independent slow-relaxation remains to be elucidated. © The Korean Society of Ginseng.

Oh W.-J.,Kyungpook National University | Endale M.,Kyungpook National University | Park J.-Y.,Kyungpook National University | Kwak Y.-S.,Korea Ginseng Corporation Central Research Institute | And 3 more authors.
Journal of Medicinal Plants Research | Year: 2011

This study was designed to investigate the activity of ethyl acetate extract from Opuntia humifusa Raf. (OH-EAE) in ligand-activated platelet aggregation. Platelet aggregation was induced either by ADP, a potent agonist to platelet G protein-coupled P2Y receptor, by collagen, a potent ligand that activates platelet integrin α2β1 and glycoprotein VI, or thrombin, a platelet protease-activated receptors subtype I and IV. The OH-EAE inhibited platelet aggregation induced by ADP (10 μM) in a dose dependent manner. In addition, OH-EAE significantly and dose-dependently inhibited collagen (2.5 μg/ml)- and thrombin (0.05 U/ml)-induced platelet aggregation. Moreover, the downstream signaling analysis revealed that the extract potently inhibited ADP-induced intracellular calcium mobilization ([Ca 2+l i). Since degranulation is a marker of platelet activation, the extract effect on the dense granule secretary activity was evaluated. As such, OH-EAE strongly suppressed ADP-induced ATP release. This preliminary result suggests that O. humifusa may be taken as a candidate lead natural compound to be considered in the search for natural products with beneficial effects on aberrant platelet activation mediated cardiovascular disorders. © 2011 Academic Journals.

Shen T.,Sungkyunkwan University | Lee J.,Chung - Ang University | Park M.H.,Ambo Institute | Lee Y.G.,Kangwon National University | And 5 more authors.
Journal of Ginseng Research | Year: 2011

Ginsenoside (G) Rp1 is a ginseng saponin derivative with anti-cancer and anti-inflammatory activities. In this study, we examined the mechanism by which G-Rp1 inhibits inflammatory responses of cells. We did this using a strategy in which DNA constructs containing cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) promoters were transfected into HEK293 cells. G-Rp1 strongly inhibited the promoter activities of COX-2 and iNOS; it also inhibited lipopolysaccharide induced upregulation of COX-2 and iNOS mRNA levels in RAW264.7 cells. In HEK293 cells G-Rp1 did not suppress TANK binding kinase 1-, Toll-interleukin-1 receptor-domain-containing adapter-inducing interferon-β (TRIF)-, TRIFrelated adaptor molecule (TRAM)-, or activation of interferon regulatory factor (IRF)-3 and nuclear factor (NF)-κB by the myeloid differentiation primary response gene (MyD88)-induced. However, G-Rp1 strongly suppressed NF-κB activation induced by IκB kinase (IKK)β in HEK293 cells. Consistent with these results, G-Rp1 substantially inhibited IKKβ-induced phosphorylation of IκBα and p65. These results suggest that G-Rp1 is a novel anti-inflammatory ginsenoside analog that can be used to treat IKKβ/NF-κB-mediated inflammatory diseases. © The Korean Society of Ginseng.

Lee S.M.,Korea Ginseng Corporation Central Research Institute | Lee H.B.,Chonnam National University | Lee C.G.,Korea University
Food Chemistry | Year: 2010

Panaxfurayne A and B, biologically novel tetrahydrofuranic polyacetylene glycosides, were founded from roots of Panax ginseng C. A. Meyer (Araliaceae). To study the contents of panaxfurynes A and B, a quantitative analysis method was developed using ultra performance liquid chromatography (UPLC) coupled with photo diode array detector. The dried (65°C, 72. h) and powdered sample (5. g) was extracted with ethanol (15%, 100. ml) for 30. min at 50°C and concentrate. The constituents were separated using an isocratic mobile phase consisting acetonitrile (16%) in water for 10. min on a ODS column. The contents of panaxfuraynes A and B in the roots of Panax quinquefolium, Panax japonicum, Panax notoginseng and P. ginseng were found to less than 3 and 2. ng/g approximately, respectively. The constituents were not detected from P. japonicum. © 2010 Elsevier Ltd.

Jeong S.C.,Daegu Haany University | Jeong Y.T.,Daegu Haany University | Lee S.M.,Korea Ginseng Corporation Central Research Institute | Kim J.H.,Daegu Haany University
Bioscience, Biotechnology and Biochemistry | Year: 2015

The immuno-modulating activities of seaweed (Hizikia fusiforme) extracts on murine macrophage and splenocyte were studied in vitro. Polysaccharide (HFP) exhibited the potential macrophage stimulating effects than water extract (HFW) such as NO production and enhanced pro-inflammatory cytokines on the Raw 264.7 cells and splenocytes. From the mono-sugar composition, HFP-associated fucose based on HFP of H. fusiforme acts as immune modulator. © 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

Lee S.M.,Korea Ginseng Corporation Central Research Institute | Kim S.C.,Korea Ginseng Corporation Central Research Institute | Oh J.,University of Mississippi | Kim J.H.,Daegu Haany University | Na M.,Chungnam National University
Phytochemistry Letters | Year: 2013

In spite of the general concept that herbal supplements are safe, there is a lack of appropriate quality control measures and regulations that often culminates in serious undesirable effects such as allergic reactions and renal and liver damage. Thus, there is a growing need to establish a suitable methodology that enables authentication and quality assurance of herbal products. The root of Panax ginseng C. A. Meyer (Araliaceae), commonly called ginseng, is traditionally recognized as a prominent herbal medicine in Far East Asia. There are two types of processed ginseng, white and red ginseng, based on processing methods, and these play a significant role in modifying ginsenosides, which are the major bioactive metabolites in these products. Herein we purify and characterize a new ginsenoside, 20(R)-ginsenoside Rf, utilizing NMR, UPLC-ESI-Q-TOF-MS and validate the metabolite is generated from its epimer, 20(S)-ginsenoside Rf during the steaming process to manufacture red ginseng. We further propose a relevant mechanism for the chemical conversion. This finding updates chemical profiling of ginseng products that can be employed in quality assurance and authentication. © 2013 Phytochemical Society of Europe.

Jung I.-C.,Hanseo University | Jeong I.S.,Chonbuk National University | Kim C.-S.,Korea Ginseng Corporation Central Research Institute
Journal of Ginseng Research | Year: 2012

Raw ginseng root of Panax ginseng is graded according to its shape and the quality of its internal tissue. A variety of grades are sold with prices according to grade. If an inferior raw ginseng is purchased, the consumer experience an economic loss. This research was conducted in order to explore the possibility of developing a noninvasive method for investigating raw ginseng's internal tissue. It has been determined that computed tomography (CT) scanner images agreed with actual cross-sections of raw ginseng. CT images were obtained to assess the internal portions of raw ginseng, and CT scans of raw ginseng were thoroughly measured using the Hounsfield unit (HU) system, since it allows for a more detailed analysis compared to nuclear magnetic resonance imaging. HU is a measure of attenuation used for CT images, with each pixel being assigned a value using a scale on which air is defined as -1000, water as 0 and compact bone as +1000. It takes about one second to process are slice and produce an image of the raw ginseng by a one channel CT scanner. An image good enough to discriminate the internal tissues can be obtained in 1/24 seconds with a one-channel CT scanner. Using this method, images of raw ginseng can be obtained and the characteristics of the internal tissues can be observed in a short time. © The Korean Society of Ginseng.

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