Time filter

Source Type

Yan-Lin S.,Kangwon National University | Wan-Geun P.,Kangwon National University | Oh-Woung K.,Korea Forest Seed and Variety Center | Soon-Kwan H.,Kangwon National University
African Journal of Biotechnology | Year: 2010

Molecular approaches are now being developed to provide a more rapid and objective identification compared to traditional phenotypic methods. Nuclear ribosomal DNA (nrDNA) targets, especially internal transcribed spacer 1 and 2 (ITS1 and ITS2), have been widely used for molecular identification of some plants and fungi. We therefore conducted an investigation in the identification of the fifth medically important Zanthoxylum schinifolium ecotypes using the common primers of the ITS region. About 620 bp fragments were obtained and the sequences of the polymerase chain reaction (PCR) products were tested. The sequence length, G+C content (%), DNA alignment and pariwise nucleotide comparisons demonstrated 98.8 to 100% sequence identities in the total ITS region, 98.3 to 100% identities in the ITS1 region and 99.5 to 100% in the ITS2 region. Comparative analysis using GenBank reference data showed that the exclusive reported data showed 100% identities with BEMR, CWDO, HCDC, JDGG and GJGD in the ITS1 region and 100% identities with thirteen ecotypes except BEMR and GRDG in the ITS2 region. The fifth different ecotypes were classified into five groups and the identification of medically important Z. schinifolium was highly improved due to the augmentation of our current ITS sequences. © 2010 Academic Journals. Source

Kwon O.-W.,Korea Forest Seed and Variety Center | Kim W.-J.,Korea forest Research Institute | Lee H.-J.,Korea forest Research Institute
Journal of the Korean Wood Science and Technology | Year: 2014

A compound has been isolated from the methanol extract of Styrax japonica bark using conventional chromatographic methods including silica gel chromatography, TLC and HPLC. The molecular formula of Styraxlignolide F analyzed by spectrometric analyses using FAB-MS, NMR was found to be C 27H34O11Na. The cytotoxicity of the styralignolide F was showed 15.2% in 1.0 ./. on human kidney cell (HEK 293). As anticancer activity of CH2Cl2 fraction, over 60% of AGS and MCF-7 cells were inhibited in concentration of 1.0 ./.. In the results of anticancer test using quantification of Bcl-2, CH2Cl2 fraction showed lower Bcl-2 and p53 expression than those of styraxlignolide F and other fractions. In apoptosis of human lung carcunoma cancer cell (A549), CH2Cl2 fraction showed the highest inhibition rate (46.9%) and styralignolide F was the next (43.5%). The CH2Cl2 fraction showed higher anti-cancer activ ities than isolated substance (styraxlignolide F), probably due to the crude extract showing synergic effects by other components. Source

Sun Y.L.,Kangwon National University | Wang D.,Kangwon National University | Lee H.B.,Kangwon National University | Park W.G.,Kangwon National University | And 3 more authors.
African Journal of Biotechnology | Year: 2011

The genus Carpinus belonging to Coryloideae, Betulaceae, has significant economic and ornamental importance. This study was undertaken with the aim to understand the genetic diversity among eighteen isolates of Carpinus turczaninovii collected from different geographical regions of Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences, to compare the infraspecificphylogenetic relationships among C. turczaninovii in Korea, and some known Carpinus plants. The size variation of sequenced rRNA ITS regions was not seen, with 215, 162, 222 bp of ITS1 region, 5.8S rRNA gene, ITS2 region, respectively. However, some certain nucleotide variations resulted in genetic diversity. In the genus Carpinus, C. turczaninovii closely genetic with Castanopsis kawakamii, Carpinus orientalis, Carpinus monbeigiana, and Calyptranthes polynenra formed one monophyletic cla de, whileCarpinus betulus and Carpinus laxiflora, respectively formed one monophyletic clade. In the present ITS sequence analysis, Carpinus fangiana, Carpinus coreana, Carpinus japonica, and Carpinus caroliniana were considered as Out Group, compared to C. turczaninovii group. The highest intraspecific variation was found between C. caroliniana and C. japonica. The least intraspecific variation was obtained between C. turczaninovii and C. coreana. The results will help further understanding Carpinus infraspecies population and their phylogenetic analysis. © 2011 Academic Journals. Source

Yan-Lin S.,Kangwon National University | Wan-Geun P.,Kangwon National University | Oh-Woung K.,Korea Forest Seed and Variety Center | Soon-Kwan H.,Kangwon National University
African Journal of Biotechnology | Year: 2010

Genus Zanthoxylum which has significant medical importance belongs to the family Rutaceae. This investigation was aimed to identify total internal transcribed spacer (ITS) regions among the nuclear ribosomal DNA (nrDNA) to distinguish Zanthoxylum piperitum from Zanthoxylum sichinifolium. The nrDNA ITS sequence markers were developed by using universal ITS5/ITS4 primer pairs. Speciesspecific primers amplified the total ITS region sequence named ITS1-YL1 and ITS1-YL2. These were amplified efficiently when paired with universal primer ITS4 in Z. piperitum, but not in Z. schinifolium. ITS1-YL1/ITS4 or ITS1-YL2/ITS4 preferential amplification was shown to be particularly useful for detection and distribution of Z. piperitum from other plant species, especially Z. schinifolium. These primers are useful to study the structure of Rutaceae family. Such identifications will be helpful for phylogenetic analysis in intraspecies population of the genus Zanthoxylum. © 2010 Academic Journals. Source

Yeom H.-J.,Korea forest Research Institute | Jung C.-S.,Korea forest Research Institute | Kang J.,Korea Institute of Toxicology | Kim J.,Gyeongsang National University | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

The fumigant and contact toxicities of 16 Asteraceae plant essential oils and their constituents against adult male and female Blattella germanica were examined. In a fumigant toxicity test, tarragon oil exhibited 100% and 90% fumigant toxicity against adult male German cockroaches at 5 and 2.5 mg/filter paper, respectively. Fumigant toxicities of Artemisia arborescens and santolina oils against adult male German cockroaches were 100% at 20 mg/filter paper, but were reduced to 60% and 22.5% at 10 mg/filter paper, respectively. In contact toxicity tests, tarragon and santolina oils showed potent insecticidal activity against adult male German cockroaches. Components of active oils were analyzed using gas chromatography, gas chromatography-mass spectrometry, or nuclear magnetic resonance spectrometer. Among the identified compounds from active essential oils, estragole demonstrated potent fumigant and contact toxicity against adult German cockroaches. β-Phellandrene exhibited inhibition of male and female German cockroach acetylcholinesterase activity with IC50 values of 0.30 and 0.28 mg/mL, respectively. © 2015 American Chemical Society. Source

Discover hidden collaborations