Yangyang, South Korea
Yangyang, South Korea

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Han S.-H.,Jeju Fisheries Research Institute | Kim J.S.,Korea Fisheries Resources Agency | Song C.B.,Jeju National University
Fisheries and Aquatic Science | Year: 2017

This is the first report of Fowlerichthys scriptissimus (Lophiiformes, Antennariidae) from Korea. A single specimen (291.0 mm SL) was collected off the coast of Jejudo Island by gill net on 28 March 2012 and identified with morphological and molecular approaches. The specimen is characterized by having all five pelvic fin rays bifurcate and possessing 20 vertebrae, 13 pectoral-fin rays, and a basidorsal ocellus on the side of the body. This species is distinguishable from other Korean taxa by the number of pectoral fin rays, the bifurcate form of the pelvic rays, and the vertebral count. We add this species to the Korean fish fauna and suggest new Korean names, "Byeol-ssin-beng-i-sok" and "Byeol-ssin-beng-i" for the genus and species, respectively. © The Author(s).


Kim H.S.,Research Institute of Fisheries Technology | Yih W.,Kunsan National University | Kim J.H.,Kunsan National University | Myung G.,Korea Fisheries Resources Agency | Jeong H.J.,Seoul National University
Ocean Science Journal | Year: 2011

The occurrence of harmful epiphytic dinoflagellates is of concern to scientists, the aquaculture industry, and government due to their toxicity not only to marine organisms but also to humans. There have been no studies on the abundance of the epiphytic dinoflagellates in Korean waters. We explored the presence of epiphytic dinoflagellates in the coastal waters off Jeju Island, southwestern Korea. Furthermore, we measured the abundance of epiphytic dinoflagellates on the thalli of 24 different macroalgae, collected from five different locations in October 2009. Five epiphytic dinoflagellate genera Amphidinium, Coolia, Gambierdiscus, Ostreopsis, and Prorocentrum were found. These five genera were observed on the thalli of the macroalgae Chordaria flagelliformis, Martensia sp., Padina arborescens, and Sargassum sp., while none were observed exceptionally on Codium fragile. The abundance of Ostreopsis spp. was highest on Derbesia sp. (8,660 cells/g wet weight), while that of Gambierdiscus spp. was highest on Martensia sp. (4,870 cells/g-ww). The maximum abundances of Amphidinium spp., Coolia spp., and Prorocentrum spp. were 410, 710, and 300 cells/g-ww, respectively. The maximum abundance of Coolia spp., Gambierdiscus spp., and Ostreopsis spp. obtained in the present study was lower than for other locations reported in literature. The results of the present study suggest that the presence and abundance of epiphytic dinoflagellates may be related to the macroalgal species of the coastal waters of Jeju Island. © 2011 Korea Ocean Research & Development Institute (KORDI) and the Korean Society of Oceanography (KSO) and Springer Netherlands.


Hong S.-E.,Gangneung - Wonju National University | Kim J.-K.,Pukyong National University | Yu J.-N.,Environmental Research Complex | Kim K.-Y.,Soonchunhyang University | And 4 more authors.
Fisheries and Aquatic Science | Year: 2012

Genetic variation in the Asian shore crab Hemigrapsus sanguineus was determined from partial mitochondrial DNA (mtDNA) sequences of the cytochrome b (Cytb) gene. Samples included 143 crabs from six localities along three coastlines in South Korea. A nucleotide sequence analysis revealed 38 variable sites in a 470-bp sequence, which defined 37 haplotypes. The haplotypes were not associated geographically and had a shallow genealogy. Pairwise FST tests and a two-dimensional scaling analysis revealed no significant genetic differentiation among most of the populations. The low pairwise comparison values, but significant genetic differentiation of a northeastern population from all other populations, might have been influenced by a restriction in gene flow caused by hydrographic conditions such as ocean boundaries. The high haplotype diversity, low nucleotide diversity, and time since H. sanguineus expansion in Korean coastal waters indicate rapid population growth and a recent, sudden expansion in the Late Pleistocene. © The Korean Society of Fisheries and Aquatic Science.


Yoon J.T.,Korea Fisheries Resources Agency | Sun S.M.,Chonnam National University | Chung G.,Chonnam National University
Journal of Applied Phycology | Year: 2014

Studies on the construction of artificial seaweed beds were carried out for the restoration of barren grounds that extend along the coast of Jeju Island in Korea. Fertilized eggs of Sargassum fulvellum and Sargassum horneri were induced from receptacles of mature plants and adhered to concrete block substrata. When the germlings that settled on the artificial substrata had grown to 3-5 mm in indoor cultures, they were moved to the ocean for intermediate cultivation, where they were cultivated under seed-cultivating nets to prevent damage from grazing animals. After the germlings had grown to 25-50 cm, the artificial substrata with settled germlings were taken out from the protective cage and transplanted on the barren grounds along the coast of Jeju Island. In the early stage of transplanting, the Sargassum thalli were partially eaten by grazing animals. Two months post-transplantation, the plants grew quickly, and their stems were harder and free from grazing damage. Most of the Sargassum had grown to over 300 cm by 6 months after transplantation on the barren grounds, and they were forming a marine forest community at the transplant sites. © 2013 Springer Science+Business Media Dordrecht.


Lee H.J.,Kongju National University | Lee D.-H.,Korea Fisheries Resources Agency | Yoon S.-J.,Korea Fisheries Resources Agency | Kim D.H.,Korea Fisheries Resources Agency | And 4 more authors.
Genes and Genomics | Year: 2013

The swimming crab, Portunus trituberculatus (Crustacea: Decapoda: Brachyura), is one of the most important fishery resources in East Asia. The aim of this work is to develop microsatellite multiplex PCR systems for 20 microsatellites which were isolated from a microsatellite-enriched genomic library and to explore the genetic diversity of P. trituberculatus captured in the coastal regions of the Yellow Sea in Korea. We prepared four multiplex PCR systems (two hexplex and tetraplex systems each) which could be used for the genotyping of 20 markers. Most markers in this system are highly informative with mean polymorphism information contents and observed heterozygosity of 0.913 and 0.930. The total number of alleles observed in this study was 724, and the mean allelic number per locus was 36.2. Significant deviations from the Hardy-Weinberg equilibrium (HWE) were observed at six loci in the Wonsando Island population, but all the loci deviated from the HWE in the Jeonjangpo population. The genetic differentiation between the two populations and the phylogenetic tree constructed by the unweighted pair group method with the arithmetic average suggested that there is no significant genetic difference between Wonsando Island and Jeonjangpo populations. The prepared multiplex PCR systems and population genetic data will be helpful to study phylogeographic analysis as well as to prepare strategies of stock management and aquaculture of P. trituberculatus. © 2013 The Genetics Society of Korea.


Choi H.J.,South Korean National Fisheries Research and Development Institute | Hwang J.Y.,South Korean National Fisheries Research and Development Institute | Choi D.L.,Korea Fisheries Resources Agency | Huh M.D.,Pukyong National University | Park M.A.,South Korean National Fisheries Research and Development Institute
Journal of Invertebrate Pathology | Year: 2012

The eggs of the Pacific oyster, Crassostraea gigas, become infertile when infected by the parasite Marteilioides chungmuensis. Histologically, M. chungmuensis infects the oyster oocyte cytoplasm, and the ovaries take on a "lumpy" appearance once infected, which lowers commercial value of the oyster. This has a negative economic impact on oyster farms in South Korea and Japan. In this study, we compared traditional diagnostic methods (histology) with two molecular-based methods (polymerase chain reaction [PCR] amplification and in situ hybridization [ISH]) to identify M. chungmuensis-infected oysters. The efficacy of PCR and ISH to identify M. chungmuensis-infected oysters was compared to that of routine histology in 100 oysters. Thirty infections were identified using PCR and 16 using histology, whereas 31 infections were identified using ISH. The ISH and PCR assays were more sensitive compared to using histology with standard epidemiological methods. We strongly recommend that early parasitic invasion should be monitored with PCR/ISH methodologies as a basis for developing effective diagnostic techniques to identify M. chungmuensis-infected oysters. © 2012 Elsevier Inc.


Hong S.,Gangneung - Wonju National University | Jin J.W.,Korea Fisheries Resources Agency | Park J.-H.,Gangneung - Wonju National University | Kim J.-K.,Pukyong National University | Jeong H.D.,Pukyong National University
Fish and Shellfish Immunology | Year: 2016

Early induction of proinflammatory cytokines is known to regulate the later immune responses to inhibit the progress of infectious diseases. In this study, proinflammatory cytokine gene expression has been studied in immune tissues to understand the early immune response induced by megalocytivirus in rock bream (Oplegnathus faciatus). For this, we have cloned interleukin (IL)-1β and IL-8 gene and performed the phylogenetic and structural analysis. Also the constitutive gene expressions of IL-1β and IL-8 were assessed in 12 organs and found to be the highest expression in tail fin and liver, respectively. The expressions of proinflammatory cytokine genes including IL-1β, IL-8, TNFα and Cox-2, and antiviral genes like Mx and IFN1 were analysed by stimulation with PAMPs and RBIV infection. In vitro study showed the highly up-regulated proinflammatory gene expressions in head kidney and the moderate up-regulation in spleen by LPS. Same concentration of polyI:C moderately upregulated IL-1β gene expression in head kidney but down-regulated IL-8 and TNFα gene expression in head kidney and spleen at 8 h. Mx and IFN1 gene expressions were highly upregulated by polyI:C in head kidney and spleen cells in vitro. By RBIV infection, proinflammatory gene expressions were initially up-regulated and later down-regulated in head kidney. In spleen, although mostly not significant, proinflammatory cytokine gene expressions were down-regulated by RBIV infection except up-regulation of Cox-2 gene expression by low concentration of RBIV at 24 h. Mx and IFN1 gene expressions were down-regulated by high dose of RBIV infection in vitro. In vivo study revealed that IL-8, TNFα, and IFN1 gene expressions were down-regulated in brain, head kidney, spleen, and gill while up-regulated in heart and liver, indicating differential proinflammatory and antiviral responses in the organs. It is supposed that down-regulation of proinflammatory gene expression in the immune organs may result in the failure of antiviral immune responses, causing high mortalities by megalocytivirus infection in rock bream. © 2015 Elsevier Ltd.


Yoon M.,Korea Fisheries Resources Agency | Jung J.-Y.,Pukyong National University | Kim D.S.,Pukyong National University
Fisheries and Aquatic Science | Year: 2013

Genetic diversity and gene flow patterns in Pollicipes mitella were investigated with a nucleotide sequence analysis of 514 base pairs from the mitochondrial cytochrome c oxidase subunit I gene (COI) in 124 samples collected from six Korean populations. In total, 59 haplotypes were defined by 40 variable nucleotide sites in the COI region. The haplotypes had shallow haplotype genealogy and no geographic associations. All populations had high haplotype diversity (0.909 to 0.979) and low nucleotide diversity (0.0055 to 0.0098). The haplotypes with recently diverged nucleotides were distributed by long-range larvae dispersal among regional populations. The pairwise fixation indices (FST) estimated with the exact test and migration rates indicate that substantial gene flow has occurred among populations as a result of sea currents, except between the Uljin (East Sea coast) and other Korean populations. This suggests that significant genetic differentiation and low migration rates have affected the Uljin population. © 2013 The Korean Society of Fisheries and Aquatic Science.


Han H.-S.,South Korean National Fisheries Research and Development Institute | Nam B.-H.,Biotechnology Research Center | Kang J.-H.,Biotechnology Research Center | Kim Y.-K.,Biotechnology Research Center | And 3 more authors.
Fisheries and Aquatic Science | Year: 2012

We used nine microsatellite DNA markers to estimate genetic variation among wild and cultured populations of the sea squirt Halocynthia roretzi. The loci were polymorphic, with 6-32 alleles, and allelic richness ranged from 6.0 to 26.1 in each population. The wild and the cultured populations had similar mean heterozygosities (H O and H E), allele numbers, and allelic richness. One cultured population with softness syndrome had a lower mean in the observed heterozygosity (H O = 0.57) and higher mean inbreeding coefficient (F IS = 0.261) than any other populations. This suggests that the loss of genetic variation in the diseased population might be due to increased inbreeding. A neighbor-joining tree and pairwise population estimates of F ST showed moderate genetic differentiation between the wild and the cultured populations. Additionally, the softness syndrome population was genetically divergent from wild populations, but it was genetically close to the cultured populations. © The Korean Society of Fisheries and Aquatic Science.


PubMed | Pukyong National University, Korea Fisheries Resources Agency and Gangneung - Wonju National University
Type: | Journal: Fish & shellfish immunology | Year: 2016

Early induction of proinflammatory cytokines is known to regulate the later immune responses to inhibit the progress of infectious diseases. In this study, proinflammatory cytokine gene expression has been studied in immune tissues to understand the early immune response induced by megalocytivirus in rock bream (Oplegnathus faciatus). For this, we have cloned interleukin (IL)-1 and IL-8 gene and performed the phylogenetic and structural analysis. Also the constitutive gene expressions of IL-1 and IL-8 were assessed in 12 organs and found to be the highest expression in tail fin and liver, respectively. The expressions of proinflammatory cytokine genes including IL-1, IL-8, TNF and Cox-2, and antiviral genes like Mx and IFN1 were analysed by stimulation with PAMPs and RBIV infection. In vitro study showed the highly up-regulated proinflammatory gene expressions in head kidney and the moderate up-regulation in spleen by LPS. Same concentration of polyI:C moderately upregulated IL-1 gene expression in head kidney but down-regulated IL-8 and TNF gene expression in head kidney and spleen at 8 h. Mx and IFN1 gene expressions were highly upregulated by polyI:C in head kidney and spleen cells in vitro. By RBIV infection, proinflammatory gene expressions were initially up-regulated and later down-regulated in head kidney. In spleen, although mostly not significant, proinflammatory cytokine gene expressions were down-regulated by RBIV infection except up-regulation of Cox-2 gene expression by low concentration of RBIV at 24 h. Mx and IFN1 gene expressions were down-regulated by high dose of RBIV infection in vitro. In vivo study revealed that IL-8, TNF, and IFN1 gene expressions were down-regulated in brain, head kidney, spleen, and gill while up-regulated in heart and liver, indicating differential proinflammatory and antiviral responses in the organs. It is supposed that down-regulation of proinflammatory gene expression in the immune organs may result in the failure of antiviral immune responses, causing high mortalities by megalocytivirus infection in rock bream.

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